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Citations for Addgene :
3251 - 3300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... plasmids were obtained from Addgene.20 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... packaging (psPAX2, Addgene #12260), and transfer (pHR-CMV-GFP ...
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bioRxiv - Molecular Biology 2024Quote: ... to envelope (VSV-G, Addgene) ratio was 10:1:0.5 ...
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bioRxiv - Genomics 2024Quote: ... which was a gift from Alexander Stark (Addgene plasmid # 99296 ; http://n2t.net/addgene:99296 ; RRID:Addgene_99296). The oligo pool was amplified using Q5 polymerase and SL1947 (5’-TCCCTACACGACGCTCTTC ...
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bioRxiv - Cancer Biology 2024Quote: ... The top 5 crRNA spacer sequences were selected and cloned into the pX459v2 Cas9 Puro Plasmid (Addgene Plasmid #62988) using single-step golden-gate cloning ...
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bioRxiv - Biochemistry 2024Quote: ... cells were transiently co-transfected for 24 h with plasmids for expression of full-length, N-terminal tagged (Flag, HA or tandem Strep tag) BRSK1/2 (or Cys-Ala mutants) and EGFP-TAU (Addgene), using 3:1 polyethylenimine (average Mw ...
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bioRxiv - Neuroscience 2024Quote: ... a second population of neurons were transfected with pAAV-CAG-GCaMP6f (Addgene). To evaluate vascular response to neuronal activity ...
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bioRxiv - Bioengineering 2024Quote: ... with packaging (psPAX2, Addgene plasmid, #12260) and envelope (pMD2.G (Addgene plasmid ...
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bioRxiv - Molecular Biology 2024Quote: dCas9: Lv EF1a dCas9-HA 2A Blast was a gift from Jerry Crabtree (Addgene plasmid # 102812 ...
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bioRxiv - Molecular Biology 2024Quote: lenti dCAS-VP64_Blast was a gift from Feng Zhang (Addgene plasmid # 61425 ; http://n2t.net/addgene:61425; RRID:Addgene_61425)
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bioRxiv - Molecular Biology 2024Quote: ... Lv EF1a dCas9-HA 2A Blast was a gift from Jerry Crabtree (Addgene plasmid # 102812 ; http://n2t.net/addgene:102812 ; RRID:Addgene_102812)
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bioRxiv - Molecular Biology 2024Quote: lenti dCAS-VP64_Blast was a gift from Feng Zhang (Addgene plasmid # 61425 ...
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bioRxiv - Molecular Biology 2024Quote: pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259 ; http://n2t.net/addgene:12259 ; RRID:Addgene_12259)
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bioRxiv - Molecular Biology 2024Quote: pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259 ...
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bioRxiv - Neuroscience 2024Quote: ... was injected due to the difficulty finding and recording VTA axons in dCA1 (pAAV-Ef1a-Flex-Axon-GCaMP7b (pAAV-Ef1a-Flex-Axon-GCaMP7b was a gift from Rylan Larsen - Addgene plasmid # 135419; http://n2t.net/addgene:135419; RRID: Addgene 135419). Following injections ...
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bioRxiv - Neuroscience 2024Quote: ... Complementary oligos were annealed and cloned into the pCFD3-dU6:3gRNA vector (Addgene #49410). The vector was introduced into vermillion (v ...
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bioRxiv - Neuroscience 2024Quote: ... Wolf Frommer (Addgene plasmid #17867).
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bioRxiv - Neuroscience 2024Quote: ... was injected due to the difficulty finding and recording VTA axons in dCA1 (pAAV-Ef1a-Flex-Axon-GCaMP7b (pAAV-Ef1a-Flex-Axon-GCaMP7b was a gift from Rylan Larsen - Addgene plasmid # 135419 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-hsyn-Flex-Axon-GCaMP6s (pAAV-hSynapsin1-FLEx-axon-GCaMP6s was a gift from Lin Tian (Addgene viral prep # 112010-AAV5 ; http://n2t.net/addgene:112010 ; RRID:Addgene 112010) was injected into the VTA of DAT-Cre+/- mice (200 nL at a depth of 4.4 mm below the surface of the dura ...
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bioRxiv - Biophysics 2024Quote: ... The synthesized gene was then cloned into the pEG BacMam expression vector (Addgene plasmid # 160683) between EcoRI and NotI restriction sites ...
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bioRxiv - Cell Biology 2024Quote: Lipofectamine-based transfections of 25 μg pEGFP-PD-L1 (Addgene) and GOLM1 (Origene ...
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bioRxiv - Cancer Biology 2024Quote: ... The mCherry-3X-NLS sequence was PCR amplified from CSII-prEF1a- mCherry-3X-NLS (Addgene #125262). PCR fragments were assembled into pLenti6.3/V5- DEST-GFP (Addgene #40125 ...
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bioRxiv - Cancer Biology 2024Quote: ... A sequence containing the 2.4-kbp p21 promoter region was PCR amplified from the pGL2-p21 promoter- Luc (Addgene #33021). The mCherry-3X-NLS sequence was PCR amplified from CSII-prEF1a- mCherry-3X-NLS (Addgene #125262) ...
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bioRxiv - Biochemistry 2024Quote: ... coli strain NiCo21(DE3) (Dr Ali Ryan, Northumbria University) containing pMSP2N2 (Addgene), and cells grown aerobically at 37 °C with 225 rpm shaking to mid-exponential phase (OD600 ∼0.6) ...
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bioRxiv - Biochemistry 2024Quote: ... A recombinant expression construct encoding the DOT1L catalytic domain (Uniprot Q8TEK3; residues 1-420) was purchased from AddGene (AddGene #36196). A recombinant expression construct encoding the Haspin kinase domain (GSG2 ...
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bioRxiv - Biochemistry 2024Quote: ... using either DH5α or JM109 (recA-, endA-, purchased from Addgene) specifically to stabilize the SPASM sensor plasmids ...
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bioRxiv - Biochemistry 2024Quote: ... and pCAG-Eco (Addgene plasmid #35617) and 3x (w/w ...
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bioRxiv - Biochemistry 2024Quote: ... pCW57.1 (a gift from David Root; Addgene plasmid # 41393), or pLenti CMV Blast DEST (706-1) ...
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bioRxiv - Biochemistry 2024Quote: ... mCherry was subcloned from H2B-mCherry (a gift from Robert Benezra; Addgene plasmid # 20972)88 into pENTR1a by flanking XhoI/XbaI sites prior to transferring to pLenti CMV Blast ...
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bioRxiv - Biochemistry 2024Quote: ... while FLAG-HA-USP18 was a gift from Dr Wade Harper (Addgene plasmid #22572). The ISG15 E1 (UBA7 ...
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bioRxiv - Biochemistry 2024Quote: ... His6-mISG15 and HA-mUbp43 were gifts from Dr Dong-Er Zhang (Addgene plasmid #12440 ...
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bioRxiv - Bioengineering 2024Quote: ... psPAX2 (Addgene #12260), and pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry (Addgene #128327 ...
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bioRxiv - Biochemistry 2024Quote: ... pNH-TrxT was a gift from Opher Gileadi (Addgene plasmid # 26106) (Savitsky et al. ...
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bioRxiv - Biochemistry 2024Quote: ... 10 μg of the plasmid encoding the Strep-tagged nucleocapsid protein was packaged with 9.183 μg of psPAX2 (Addgene #12260) and 2.768 μg of pVSV-G (Addgene #138479 ...
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bioRxiv - Biochemistry 2024Quote: ... The tag-free wildtype (Wuhan-hu-1) nucleocapsid protein plasmid was purchased from Addgene (#177937), and the plasmid encoding the Strep-tagged nucleocapsid protein was a kind gift from Dr ...
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bioRxiv - Biochemistry 2024Quote: ... signal amplified by PCR and inserted downstream of the tetracycline-responsive promoter between cut sites NheI and SgfI in the HP138 puro plasmid (Addgene plasmid: 134246). MORC2 constructs were amplified by PCR and inserted downstream of TetO ...
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bioRxiv - Genomics 2024Quote: ... and pMD2.G (psPAX2 and pMD2.G were a gift from Didier Trono, Addgene plasmid # 12260 ; http://n2t.net/addgene:12260 ; RRID:Addgene_12260) were obtained from Addgene ...
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ZNF143 binds DNA and stimulates transcription initiation to activate and repress direct target genesbioRxiv - Genomics 2024Quote: ... We generated a linear homology-directed repair donor by amplifying the pCRIS-PITChv2-dTAG-Puro plasmid (Addgene #91796) with the primers in Table 1 (Nabet et al ...
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bioRxiv - Genomics 2024Quote: We modified pLentiRNACRISPR_007 (Addgene 138149 ...
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bioRxiv - Genomics 2024Quote: ... VSV G pseudotyped lentivirus was prepared at the OHSU virology core using pHR-hSyn-eGFP plasmid (Addgene plasmid #114215) with a titer of 1.0x109 TU/mL ...
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bioRxiv - Biochemistry 2024Quote: ... Cells received either 500 ng 6x-FkhP (6x-DBE) obtained from Addgene 45 or 500 ng 6x-FkhP with 500 ng pCS2 Flag-FOXC2 WT from Addgene 45 or mutant FOXC2 generated as described above ...
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bioRxiv - Neuroscience 2024Quote: ... GCaMP6s (Addgene plasmid 40753 ...
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bioRxiv - Genomics 2024Quote: ... and pCas9_GFP (Addgene 44719) (Ding et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... Constitutive plant promoters and terminators were purchased from Addgene as part of the MoClo Toolbox for Plants58 ...
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bioRxiv - Microbiology 2024Quote: ... and subcloned into pLenti-CMV-GFP-Puro (Addgene Plasmid #17448). pHAGE2-EF1aInt-TMPRSS2-IRES-mCherry plasmid was kindly provided by Dr ...
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bioRxiv - Plant Biology 2024Quote: ... Binomica Labs) with primers for Golden Gate assembly into the Level 1 pICH47742 plasmid (Addgene catalog # 48001) with a double 35S promoter (pICH51277 ...
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bioRxiv - Cell Biology 2024Quote: ... lentiCas-Blast plasmid (Addgene, 52962) was co-transfected with third-generation lentiviral packaging plasmids (pMDLg/pRRE ...
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bioRxiv - Cancer Biology 2024Quote: ... The mAID-mCherry cassette was amplified from Addgene plasmid 72830 (pMK292)45 and mAID-HaloTag was amplified from Addgene plasmid 112852102 ...
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bioRxiv - Cancer Biology 2024Quote: ... PsPAX2 (Addgene 12260), and VSV-G (Addgene 12259) ...
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bioRxiv - Cancer Biology 2024Quote: ... and VSV-G (Addgene 12259). ViralBoost (Alstembio VB100 ...