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3251 - 3300 of 10000+ citations since 2020

• Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide release

  Xuehan Zhou, et al., bioRxiv - Neuroscience 2024

  Quote: ... flanked by HindIII and NotI restriction sites to be subsequently cloned into pCMV vector (Addgene #111053). For sensor optimization ...
• A novel interaction between the 5’ untranslated region of the Chikungunya virus genome and Musashi RNA binding protein is essential for efficient virus genome replication

  Kaiwen Sun, et al., bioRxiv - Microbiology 2024

  Quote: ... Ryder (Addgene plasmid # 60356 ...
• A novel interaction between the 5’ untranslated region of the Chikungunya virus genome and Musashi RNA binding protein is essential for efficient virus genome replication

  Kaiwen Sun, et al., bioRxiv - Microbiology 2024

  Quote: ... Ryder (Addgene plasmid # 60356; http://n2t.net/addgene:60356; RRID: Addgene_60356) (20) ...
• Effective in vivo binding energy landscape illustrates kinetic stability of RBPJ-DNA binding

  Duyen Huynh, et al., bioRxiv - Biophysics 2024

  Quote: ... and the virus envelope plasmid pMD2.G (Addgene #12259) using JetPRIME (Polyplus-transfection ...
• Effective in vivo binding energy landscape illustrates kinetic stability of RBPJ-DNA binding

  Duyen Huynh, et al., bioRxiv - Biophysics 2024

  Quote: ... the plasmid pLV-tetO-Oct4 (Addgene #19766)69 including the sequence of interest ...
• Effective in vivo binding energy landscape illustrates kinetic stability of RBPJ-DNA binding

  Duyen Huynh, et al., bioRxiv - Biophysics 2024

  Quote: ... annealed and ligated the oligos into the px459 v2.0 (Addgene #62988)61 predigested with BbsI ...
• Effective in vivo binding energy landscape illustrates kinetic stability of RBPJ-DNA binding

  Duyen Huynh, et al., bioRxiv - Biophysics 2024

  Quote: ... the packaging plasmid psPAX2 (Addgene #12260) and the virus envelope plasmid pMD2.G (Addgene #12259 ...
• Antibody-lectin chimeras for glyco-immune checkpoint blockade

  Jessica C. Stark, et al., bioRxiv - Bioengineering 2024

  Quote: ... Lentiviral vector encoding HER2/neu was a gift from Mien-Chie Hung (Addgene plasmid #16257). Stable HER2+ cell lines were generated as previously described80 and HER2 expression was verified by flow cytometry ...
• Inhibition of O-GlcNAc transferase activates type I interferon-dependent antitumor immunity by bridging cGAS-STING pathway

  Jianwen Chen, et al., bioRxiv - Cancer Biology 2024

  Quote: ... The pWPXLd-OGT-GFP fusion vector was described in our previous articles,(2) pET24a-ncOGT-FL (190821, Addgene), other plasmids were cloned into pcDNA3.1 backbone with c-myc or HA tag ...
• Netrin signaling mediates survival of dormant epithelial ovarian cancer cells

  Pirunthan Perampalam, et al., bioRxiv - Cancer Biology 2024

  Quote: Plasmids encoding Netrin1 and Netrin3 were obtained from Addgene (Ntn1-Fc-His ...
• HaloTag display enables quantitative single-particle characterization and functionalization of engineered extracellular vesicles

  Roxana E. Mitrut, et al., bioRxiv - Bioengineering 2024

  Quote: ... a gift from Veit Hornung (Addgene, plasmid #80960),35 was genetically fused at the C-terminus to a validated PDGFR transmembrane domain36 and was cloned into a pGIPZ lentiviral expression vector (Open Biosystems ...
• "Cold" Orthogonal Translation by Psychrophilic Pyrrolysyl-tRNA Synthetase Boosts Genetic Code Expansion

  Nikolaj G. Koch, et al., bioRxiv - Synthetic Biology 2024

  Quote: The pTECH vector was a gift from Dieter Söll (Addgene plasmid #104073).16 Genes were ordered as codon optimized fragments from Twist Bioscience ...
• A highly conserved neuronal microexon in DAAM1 controls actin dynamics, RHOA/ROCK signaling, and memory formation

  Patryk Poliński, et al., bioRxiv - Neuroscience 2024

  Quote: ... HIV-1 Rev (pRSV-Rev Addgene: 12253), and VSV g-glycoprotein Env (pMD2.G Addgene ...
• A highly conserved neuronal microexon in DAAM1 controls actin dynamics, RHOA/ROCK signaling, and memory formation

  Patryk Poliński, et al., bioRxiv - Neuroscience 2024

  Quote: ... together with pLentiRhoA2G (Addgene: 40179). Media was changed after 6 h to neuronal N2 media ...
• A highly conserved neuronal microexon in DAAM1 controls actin dynamics, RHOA/ROCK signaling, and memory formation

  Patryk Poliński, et al., bioRxiv - Neuroscience 2024

  Quote: ... and VSV g-glycoprotein Env (pMD2.G Addgene: 12259), together with pLentiRhoA2G (Addgene ...
• The complexity and dynamics of in organello translation assessed by high-resolution mitochondrial ribosome profiling

  Taisei Wakigawa, et al., bioRxiv - Molecular Biology 2024

  Quote: ... 59 and cloned into the pL-CRISPR.EFS.GFP (a gift from Benjamin Ebert, Addgene plasmid # 57818; http://n2t.net/addgene:57818; RRID: Addgene_57818) and pL-CRISPR.EFS.tRFP (a gift from Benjamin Ebert ...
• The complexity and dynamics of in organello translation assessed by high-resolution mitochondrial ribosome profiling

  Taisei Wakigawa, et al., bioRxiv - Molecular Biology 2024

  Quote: ... and pL-CRISPR.EFS.tRFP (a gift from Benjamin Ebert, Addgene plasmid # 57819 ...
• The complexity and dynamics of in organello translation assessed by high-resolution mitochondrial ribosome profiling

  Taisei Wakigawa, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Two guide RNA sequences were selected to target exon 1 of mtIF3 as described previously (5′-GCAAUAGGGGACAACUGUGC-3′ and 5′-GCAGAGUAUCAGCUCAUGAC-3′) 59 and cloned into the pL-CRISPR.EFS.GFP (a gift from Benjamin Ebert, Addgene plasmid # 57818 ...
• The complexity and dynamics of in organello translation assessed by high-resolution mitochondrial ribosome profiling

  Taisei Wakigawa, et al., bioRxiv - Molecular Biology 2024

  Quote: ... A guide RNA sequence was selected to target exon 1 of MTU1 and cloned into LentiCRISPR v2 (a gift from Feng Zhang, Addgene plasmid # 52961; http://n2t.net/addgene:52961; RRID: Addgene_52961) 131 ...
• Mutant mice lacking alternatively spliced p53 isoforms unveil Ackr4 as a male-specific prognostic factor in Myc-driven B-cell lymphomas

  Anne Fajac, et al., bioRxiv - Cancer Biology 2024

  Quote: ... annealed and cloned in the Cas9 expression vector pSpCas9(BB)-2A-Puro (PX459) (Addgene). Prelimary tests for efficiency to induce cleavage in the targeted DNA regions were performed in HEK293T cells ...
• Prominin 1 and Tweety Homology 1 both induce extracellular vesicle formation

  Tristan A. Bell, et al., bioRxiv - Biochemistry 2024

  Quote: ... mTagBFP2 was subcloned from pBAD (Addgene #54572, a gift from Michael Davidson) into a pET28a vector with a C-terminal Strep tag for bacterial overexpression ...
• Prominin 1 and Tweety Homology 1 both induce extracellular vesicle formation

  Tristan A. Bell, et al., bioRxiv - Biochemistry 2024

  Quote: ... Ttyh1 was expressed from a pLX304 vector after addition of C-terminal Strep and His tags to an existing construct (Addgene #161676, a gift from Mike McManus). To stably express fluorescently tagged Prom1 WT and W795R variants in HeLa cells for live cell imaging ...
• Inhibition of host N-myristoylation compromises the infectivity of SARS-CoV-2 due to Golgi-bypassing egress from lysosomes and endoplasmic reticulum

  Saber H. Saber, et al., bioRxiv - Microbiology 2024

  Quote: ... A549 cells were transfected with 2 µg DNA of ARF1-GFP48 (ARF1-GFP was a gift from Paul Melancon, Addgene plasmid #39554; http://n2t.net/addgene:39554; RRID:Addgene_39554), Hsp47-GFP-KDEL51 (a gift from Eija Jokitalo ...
• Inhibition of host N-myristoylation compromises the infectivity of SARS-CoV-2 due to Golgi-bypassing egress from lysosomes and endoplasmic reticulum

  Saber H. Saber, et al., bioRxiv - Microbiology 2024

  Quote: ... A549 cells were transfected with 2 µg DNA of ARF1-GFP48 (ARF1-GFP was a gift from Paul Melancon, Addgene plasmid #39554 ...
• Ion channel inhibition by targeted recruitment of NEDD4-2 with divalent nanobodies

  Travis J. Morgenstern, et al., bioRxiv - Biophysics 2024

  Quote: ... the HECT domain consisting of residues 615-994 of full-length NEDD4-2 (a gift from Joan Massague, Addgene plasmid # 27000) (66 ...
• Lineage-dependence of the neuroblastoma surfaceome defines tumor cell state-dependent and independent immunotherapeutic targets

  Nathan M. Kendsersky, et al., bioRxiv - Cancer Biology 2024

  Quote: ... we utilized the pLenti-CMV-TetR-blastR construct (Addgene cat. 17492). Both pLenti-CMV/TO-AXL-puroR and pLenti-CMV-TetR-blastR constructs were independently packaged into lentiviral particles ...
• Compositionally unique mitochondria in filopodia support cellular migration

  Madeleine Marlar-Pavey, et al., bioRxiv - Cell Biology 2024

  Quote: ... Myo19-GFP (Addgene 134988) was a gift from Martin Bähler (64) ...
• Traveling wave chemotaxis of neutrophil-like HL-60 cells

  Motohiko Ishida, et al., bioRxiv - Cell Biology 2024

  Quote: ... and pLL7.0:hITSN1(1159-1509)- tgRFPt-SspB (Addgene #60419 a gift from Prof. Brian Kuhlman), respectively(Guntas et al. ...
• Deletion of FNDC5/Irisin modifies murine osteocyte function in a sex-specific manner

  Anika Shimonty, et al., bioRxiv - Physiology 2024

  Quote: ... was obtained from Addgene (105542), and packaged at the UPenn Vector Core to a titer of 2.10 × 1013 GC per ml39 ...
• Engineered 3D Immuno-Glial-Neurovascular Human miBrain Model

  Alice E. Stanton, et al., bioRxiv - Neuroscience 2024

  Quote: NGN2-neurons were differentiated from NGN2-iPSCs through the 7-day protocol and then transfected with either pAAV-CAG-hChR2(H134R)-mCherry (Addgene) or pAAV-CAG-tdTomato (Addgene) ...
• Specific interactions with phospho-Ubls and allosteric conformational changes regulate the E3 ligase activity of Parkin

  Dipti Ranjan Lenka, et al., bioRxiv - Biochemistry 2024

  Quote: ... Pediculus humanus corporis PINK1 (115-575) was a gift from David Komander 34(Addgene plasmid # 110750). Ube1 was a gift from Cynthia Wolberger 35(Addgene plasmid # 34965).
• Deletion of FNDC5/Irisin modifies murine osteocyte function in a sex-specific manner

  Anika Shimonty, et al., bioRxiv - Physiology 2024

  Quote: ... plus a five-amino-acid linker plus a C-terminal flag tag was cloned into the pENN.AAV.CB7.CI.pm20d1flag.WPRE.rBG vector (Addgene plasmid no. 132682). AAV8-GFP (pENN.AAV.CB7.CI.eGFP.WPRE.rBG) ...
• Collapse of late endosomal pH elicits a rapid Rab7 response via V-ATPase and RILP

  R.J. Mulligan, et al., bioRxiv - Cell Biology 2024

  Quote: ... pCAG-Cre-GFP (Addgene #13776, Cepko Lab), pCDNA3-2xHA-V1G1 (gift from Dr ...
• Collapse of late endosomal pH elicits a rapid Rab7 response via V-ATPase and RILP

  R.J. Mulligan, et al., bioRxiv - Cell Biology 2024

  Quote: ... pET-His-GST-tev-LIC (Addgene #29655, Gradia Lab), pGEX-4T-3-mR7BD (Addgene #79149 ...
• Collapse of late endosomal pH elicits a rapid Rab7 response via V-ATPase and RILP

  R.J. Mulligan, et al., bioRxiv - Cell Biology 2024

  Quote: Emerald-Rab7WT (Addgene #54244, Davidson Lab), GFP-Rab7WT (Addgene #12605 ...
• Collapse of late endosomal pH elicits a rapid Rab7 response via V-ATPase and RILP

  R.J. Mulligan, et al., bioRxiv - Cell Biology 2024

  Quote: ... pmRFP-LC3 (Addgene #21075, Yoshimori Lab). To generate Emerald-Rab7L8A ...
• Collapse of late endosomal pH elicits a rapid Rab7 response via V-ATPase and RILP

  R.J. Mulligan, et al., bioRxiv - Cell Biology 2024

  Quote: ... GFP-Rab7WT (Addgene #12605, Pagano Lab), GFP-Rab7Q67L (gift from Dr ...
• Collapse of late endosomal pH elicits a rapid Rab7 response via V-ATPase and RILP

  R.J. Mulligan, et al., bioRxiv - Cell Biology 2024

  Quote: ... pGEX-4T-3-mR7BD (Addgene #79149, Edinger Lab), mCherry (Clontech) ...
• Tumor-infiltrating nerves functionally alter brain circuits and modulate behavior in a mouse model of head-and-neck cancer

  Jeffrey Barr, et al., bioRxiv - Cancer Biology 2024

  Quote: ... A 10-μl Hamilton syringe was used to infuse 1 μl of AAV1/Syn-GCaMP6f-WPRESV40 (titer 4.65 × 1013 GC per ml, via Addgene) into the parabrachial nucleus (−5.3 mm anteroposterior ...
• Bam complex associated proteins in Escherichia coli are functionally linked to peptidoglycan biosynthesis, membrane fluidity and DNA replication

  Jack A. Bryant, et al., bioRxiv - Microbiology 2024

  Quote: ... coli LC-E18 and the pSGRNA plasmid was a gift from David Bikard (Addgene plasmid # 115924) and has been described previously [110] ...
• p53 secures the normal behavior of H3.1 histone in the nucleus by regulating nuclear phosphatidic acid and EZH2 during the G1/S phase

  Tsukasa Oikawa, et al., bioRxiv - Cell Biology 2024

  Quote: ... and Cas9 (modified based on pX330A-1x2, Addgene #58766, and pX330S-2-PITCh, Addgene #63670) (Sakuma et al. ...
• Increased S6K1 phosphorylation protects against early steps of Tau aggregation under long-term mitochondrial stress

  Lukasz Samluk, et al., bioRxiv - Cell Biology 2024

  Quote: ... VN-Tau (wt) was a gift from Tiago Outeiro (Addgene plasmid no. 87368; http://n2t.net/addgene:87368; RRID:Addgene_87368; (Blum et al., 2015). Tau (wt)-VC was a gift from Tiago Outeiro (Addgene plasmid no ...
• p53 secures the normal behavior of H3.1 histone in the nucleus by regulating nuclear phosphatidic acid and EZH2 during the G1/S phase

  Tsukasa Oikawa, et al., bioRxiv - Cell Biology 2024

  Quote: ... and Cas9 (modified based on pX330A-1x2, Addgene #58766 ...
• Two-way Dispatched function in Sonic hedgehog shedding and transfer to high-density lipoproteins

  K. Ehring, et al., bioRxiv - Cell Biology 2024

  Quote: ... NanoLuc (pNH-NanoLuc, Plasmid #173075, Addgene), flanked by one glycine residue on both sides ...
• Proteomic landscape of tunneling nanotubes reveals CD9 and CD81 tetraspanins as key regulators

  Roberto Notario Manzano, et al., bioRxiv - Cell Biology 2024

  Quote: ... The corresponding guide DNA sequences were cloned into the lentiCRISPRv2 plasmid (#52961; Addgene) according to the instructions of the Zhang laboratory (https://www.addgene.org/52961/) ...
• Proteomic landscape of tunneling nanotubes reveals CD9 and CD81 tetraspanins as key regulators

  Roberto Notario Manzano, et al., bioRxiv - Cell Biology 2024

  Quote: ... U2OS stably expressing H2B-GFP (Addgene 11680) and actin chromobody GFP (pAC-TagGFP from Chromotek ...
• A MSTN^Del273C mutation with FGF5 knockout sheep by CRISPR/Cas9 promotes skeletal muscle myofiber hyperplasia

  Ming-Ming Chen, et al., bioRxiv - Cell Biology 2024

  Quote: The Cas9 and U6-sgRNA co-expression vector backbones pX330 were purchased from Addgene (plasmid ID: 42230 and 48138). Sheep MSTN and FGF5 sgRNAs were designed using CRISPR Design Tool (http://tools.genome-engineering.org) ...
• Keratin isoform shifts modulate motility signals during wound healing

  Benjamin A Nanes, et al., bioRxiv - Cell Biology 2024

  Quote: ... pLKO.1-Scrambled (Addgene #136035) 100 was modified to express H2B-mRuby3 for visual identification of transduced cells based on a nuclear fluorescence signal101 ...
• DYRK1A Interacts with the Tuberous Sclerosis Complex and Promotes mTORC1 Activity

  Pinhua Wang, et al., bioRxiv - Molecular Biology 2024

  Quote: ... plasmids were procured from Addgene. For expressing cDNA using lentivirus ...
• DYRK1A Interacts with the Tuberous Sclerosis Complex and Promotes mTORC1 Activity

  Pinhua Wang, et al., bioRxiv - Molecular Biology 2024

  Quote: pcDNA3 -HA3-TSC1 (Yue Xiong; Addgene #19911), pcDNA3 Flag TSC2 (Brendan Manning ...