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Citations for Addgene :
3051 - 3100 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and 1μg of pDEST-CMV mCherry-GFP-LC3B WT (Addgene plasmid # 123230; http://n2t.net/addgene:123230; RRID:Addgene_123230, Agrotis & Ketteler)30 per well using X-tremeGene HP (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... cells were co-transfected with 900 ng psPAX2 (psPAX2 was a gift from Didier Trono (Addgene plasmid #12260 ...
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bioRxiv - Cell Biology 2024Quote: ... Lentiviral construct UT4GEPIR (Addgene #186712) was used for cloning Dox-inducible Ascl1 and mScarlet constructs ...
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bioRxiv - Cell Biology 2024Quote: ... and 1μg of pDEST-CMV mCherry-GFP-LC3B WT (Addgene plasmid # 123230 ...
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bioRxiv - Cell Biology 2024Quote: ... 100 ng pMD2.G (pMD2.G was a gift from Didier Trono (Addgene plasmid #12259; http://n2t.net/addgene:12259; RRID:Addgene_12259)) and 1μg of pDEST-CMV mCherry-GFP-LC3B WT (Addgene plasmid # 123230 ...
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bioRxiv - Developmental Biology 2024Quote: ... 2013) (pDD162 was a gift from Bob Goldstein (Addgene plasmid # 47549; http://n2t.net/addgene:47549; RRID:Addgene_47549)) ...
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bioRxiv - Cell Biology 2024Quote: ... The sequences were subsequently cloned in the destination vector MAC-tag-C, a gift from Markku Varjosalo (Liu et al., 2018) (Addgene plasmid # 108077 ...
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bioRxiv - Cancer Biology 2024Quote: sgRNA sequences were designed using CRISPick38,39 and cloned into pSpCas9(BB)-2A-GFP (PX458) (Addgene, #48138). A549 cells were transfected with these vectors using Lipofectamine 2000 transfection reagent (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... The sequences were subsequently cloned in the destination vector MAC-tag-C, a gift from Markku Varjosalo (Liu et al., 2018) (Addgene plasmid # 108077; http://n2t.net/addgene:108077; RRID:Addgene_108077), using the Gateway™ LR Clonase™ II Enzyme mix (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial), a gift from Adam Cohen (Werley et al., 2020) (Addgene plasmid # 163076 ...
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bioRxiv - Cell Biology 2024Quote: ... Alice Ting (Han et al., 2017) (Addgene plasmid # 129705 ...
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bioRxiv - Neuroscience 2024Quote: ... GCaMP6s (Addgene plasmid 40753 ...
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bioRxiv - Genomics 2024Quote: ... and pCas9_GFP (Addgene 44719) (Ding et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... Constitutive plant promoters and terminators were purchased from Addgene as part of the MoClo Toolbox for Plants58 ...
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bioRxiv - Microbiology 2024Quote: ... and subcloned into pLenti-CMV-GFP-Puro (Addgene Plasmid #17448). pHAGE2-EF1aInt-TMPRSS2-IRES-mCherry plasmid was kindly provided by Dr ...
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bioRxiv - Plant Biology 2024Quote: ... Binomica Labs) with primers for Golden Gate assembly into the Level 1 pICH47742 plasmid (Addgene catalog # 48001) with a double 35S promoter (pICH51277 ...
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bioRxiv - Cancer Biology 2024Quote: ... Clones expressing EGFP (FUGW virus; Addgene #14883) and TdTomato (FUtdTW virus ...
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bioRxiv - Cell Biology 2024Quote: ... The third generation HIV-1 derived lentiviral vector LeGO-iG2-Puro+-Luc2 expresses the Luc2 variant of firefly luciferase (cloned from AddGene Plasmid 24337) under the control of an SFFV-promoter ...
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bioRxiv - Cell Biology 2024Quote: ... pTH726-CEN-RLuc/minCFLuc and pTH727-CEN-RLuc/staCFLuc were a gift from Tobias von der Haar (Addgene plasmid # 38210 and 38211). Egd1 and Egd2 overexpression plasmids are under the control of their endogenous promoters (p413-Egd1 ...
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bioRxiv - Cancer Biology 2024Quote: ... and pMD2.G (Addgene plasmid # 12259) were a gift from Didier Trono.
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bioRxiv - Cancer Biology 2024Quote: ... was cloned into MSCV-IRES-KSR1-RFP and PEI transfected into HEK293T cells along with pUMVC retroviral packaging construct (Addgene #8449) and CMV5-VSVG envelope construct (Addgene #8454) ...
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bioRxiv - Cancer Biology 2024Quote: Murine KSR1 (resistant to degradation by sgRNA sequences targeting human KSR1) was cloned into MSCV-IRES-KSR1-RFP (Addgene #33337) and PEI transfected into HEK293T cells along with pUMVC retroviral packaging construct (Addgene #8449 ...
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bioRxiv - Cell Biology 2024Quote: HSP90B1 constructs: pDONR223_HSP90B1_WT was purchased from Addgene (Plasmid #82130) and Gibson assembly was used to insert a 3xFLAG tag just after the ER signal sequence ...
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bioRxiv - Cancer Biology 2024Quote: ... and PEI transfected into HEK293T cells along with pUMVC retroviral packaging construct (Addgene #8449) and CMV5-VSVG envelope construct (Addgene #8454) ...
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bioRxiv - Cancer Biology 2024Quote: Guide RNA (gRNA) sequences targeting neutral sphingomyelinases 1 & 2 and Rab27s a and b were cloned into a lentiCRISPR vector (Addgene (52961) 45 ...
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bioRxiv - Cancer Biology 2024Quote: ... and 3μg pMD2.G (Addgene, 12259) using Xfect Transfection Reagent (Takara ...
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bioRxiv - Cancer Biology 2024Quote: ... and pMD2.G envelope construct (Addgene #12259). Lentivirus containing media was harvested at 72-h ...
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bioRxiv - Cancer Biology 2024Quote: ... and pX330-gRNA (Addgene # 158973) plasmids with Lipofectamine 2000 ...
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bioRxiv - Cell Biology 2024Quote: The MPV17-HA plasmid was obtained by cloning the MPV17 CDS into the pKH3, a gift from Ian Macara (Mattingly & Macara, 1996) (Addgene plasmid # 12555 ...
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bioRxiv - Cell Biology 2024Quote: ... 200-800 bases apart were designed using the Synthego Knockout Guide Design tool (http://design.synthego.com) and cloned into pSpCas9(BB)-2A-GFP (PX458; Addgene #48138) and pSpCas9(BB)-2A-mCherry ...
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bioRxiv - Cell Biology 2024Quote: ... Alice Ting (Han et al., 2017) (Addgene plasmid # 129705; http://n2t.net/addgene:129705; RRID:Addgene_129705). All sequences were cloned in a pDONR201 donor plasmid ...
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bioRxiv - Cell Biology 2024Quote: The MPV17-HA plasmid was obtained by cloning the MPV17 CDS into the pKH3, a gift from Ian Macara (Mattingly & Macara, 1996) (Addgene plasmid # 12555; http://n2t.net/addgene:12555; RRID:Addgene_12555). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial), a gift from Adam Cohen (Werley et al., 2020) (Addgene plasmid # 163076; http://n2t.net/addgene:163076; RRID:Addgene_163076). The sequence of the construct was cloned into pKH3 plasmid for its use in transfection experiment for mammalian expression ...
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bioRxiv - Cancer Biology 2024Quote: ... pLenti-trGluc (Addgene # 158959), and pX330-gRNA (Addgene # 158973 ...
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bioRxiv - Cancer Biology 2024Quote: Lentivirus particles were generated in HEK293T cells following transfection with the CAR plasmid along with pMD2.G envelope (Addgene, Cat.12259) and psPAX2 packaging plasmids (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... a gift from Cindy Miranti (Addgene plasmid # 85966; https://www.addgene.org/85966; RRID: Addgene_85966). shRNA targeting sequences (with RNAi consortium ID ...
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bioRxiv - Cancer Biology 2024Quote: ... a gift from Cindy Miranti (Addgene plasmid # 85966 ...
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bioRxiv - Cancer Biology 2024Quote: ... a gift from Bob Weinberg (Addgene plasmid # 8453; http://https://www.addgene.org/8453; RRID: Addgene_8453). For doxycycline-induced knockdown of UTX/KDM6A ...
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bioRxiv - Cancer Biology 2024Quote: ... Atg7 knockout cell lines were generated by transducing cells with lentiviral CRISPR/Cas9 (TLCV2 Addgene plasmid #87360). Lentiviruses were generated using 293T cells via transfection with PEI ...
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bioRxiv - Cancer Biology 2024Quote: ... 9μg psPAX2 (Addgene, 12260) and 3μg pMD2.G (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: U2OS cells infected with lentivirus expressing shRNA targeting “scramble” control sequence (AddGene Plasmid #1864 ...
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bioRxiv - Cancer Biology 2024Quote: ... The empty vector construct used for 293T experiments was pCMV-Neo-Bam (Addgene 16440), which has the same major functional elements as pcDNA3.1 with the exception of an HSV TK promoter for the Neo resistance gene in place of an SV40 promoter ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentivirus was packaged in 293T cells cotransfected with pHEF-VSVG (Addgene#22501) and pPax2 (Addgene#12259 ...
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bioRxiv - Cancer Biology 2024Quote: ... The corresponding empty vector pMY-IRES-EGFP was purchased from Addgene (163361). Plasmid sequences were verified via Whole Plasmid Sequencing by Plasmidsaurus/Primordium Labs using Oxford Nanopore Technology with custom analysis and annotation ...
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bioRxiv - Cancer Biology 2024Quote: ... and pPax2 (Addgene#12259) in PEI transfection reagent ...
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bioRxiv - Cancer Biology 2024Quote: ... and psPAX2 packaging plasmids (Addgene, Cat.12260), using extreme Xp transfection reagent ...
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bioRxiv - Cell Biology 2024Quote: ... mRFP-FKBP from Tamas Balla (Addgene plasmid #67514); Reticulon 4-GFP and GFP-Climp63 from J ...
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bioRxiv - Cell Biology 2024Quote: ... Schirmer (Addgene plasmid #62008); mRFP-FKBP from Tamas Balla (Addgene plasmid #67514) ...
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bioRxiv - Cell Biology 2024Quote: ... Hell (Addgene plasmid #52669); EGFP-MyosinIIA from M ...
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bioRxiv - Cell Biology 2024Quote: ... 1 × 106 cells were plated in 60 mm dishes for 24 hours followed by transfection with m6A-Tracer GFP (AddGene, 139403) and DAM-lamin B1 (AddGene ...