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Citations for Addgene :
3401 - 3450 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and rAAV-444h-minBG-mTFP1-WPRE3-BGHpA (Addgene plasmid #191729, alias AiP12965). To investigate Cre- and Flp-dependent intersectional strategies for striosome labeling ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-TREtight-mTagBFP2-B19G (Addgene 100799) has been described 146 ...
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bioRxiv - Microbiology 2024Quote: ... The pNCS-mClover3 was a gift from Michael Lin (Addgene-plasmid #74236).
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bioRxiv - Neuroscience 2024Quote: ... EnvA-enveloped rabies virus RVΔG-4mCherry (EnvA) 147 was produced as described previously 148–150 but using helper plasmids pCAG-B19N (Addgene #59924), pCAG-B19P (Addgene #59925) ...
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bioRxiv - Neuroscience 2024Quote: ... inside the FRT-flanking region of a CAG-promoter plasmid (Addgene #99133). Electroporation into cortical neurons was performed applying 4 pulses of 42 V for 50 ms with 500 ms intervals ...
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bioRxiv - Molecular Biology 2024Quote: ... packaging (pMD2.G, Addgene #12259)115 ...
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bioRxiv - Molecular Biology 2024Quote: ... The PE5max plasmid (Addgene #174828) encodes an S ...
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bioRxiv - Neuroscience 2024Quote: rAAV2-retro-CAG-H2B-mGreenLantern (Addgene#177332) was produced at the University of Miami viral core facility at the Miami Project to Cure Paralysis ...
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bioRxiv - Molecular Biology 2024Quote: ... which was a kind gift from Eric Lander & David Sabatini (Addgene plasmid #50661 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-hSyn-dLight1.1 was a gift from Lin Tian (Addgene plasmid #111066; http://n2t.net/addgene:111066; RRID:Addgene_111066).
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bioRxiv - Neuroscience 2024Quote: ... The Cre-dependent red excitatory opsin pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 in AAV9 serotype was purchased from Addgene (#124651-AAV9) with a titer of ≥5 x1012 vg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... The plasmid pAAV-hSyn-dLight1.1 was purchased from Addgene (#111066) and packaged into AAV9 serotype in-house to a titer of 7.7 x 1012 vg/mL (protocol described below) ...
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bioRxiv - Neuroscience 2024Quote: ... Wilson (Addgene plasmid #112865; http://n2t.net/addgene:112865; RRID:Addgene_112865). pAAV-hSyn-dLight1.1 was a gift from Lin Tian (Addgene plasmid #111066 ...
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bioRxiv - Molecular Biology 2024Quote: ... pAdDeltaF6 helper (a gift from J. M. Wilson, Addgene, #112867) and an AAV transfer plasmid containing an overexpression construct or sgRNA expression cassettes ...
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bioRxiv - Neuroscience 2024Quote: HEK293FT cells were transfected using calcium phosphate transfection with lentiviral constructs and the associated packaging plasmids psPAX2 (a gift of Didier Trono, Addgene #12260) and pMD2.G (a gift of Didier Trono ...
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bioRxiv - Neuroscience 2024Quote: ... and pMD2.G (a gift of Didier Trono, Addgene 12259). 16 hours after transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were transfected with the plasmid of interest (mentioned in ‘Cloning of H3S10 variant plasmids’) and the lentiviral packaging plasmid pCMV-VSV-G a gift from Bob Weinberg (Addgene plasmid #8454 ...
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bioRxiv - Neuroscience 2024Quote: ... AAV1-syn-FLEX-jGCaMP8f-WPRE (162379-AAV1, Addgene, MA, USA), CAV-mCherry and CAV-PRS-Cre-V5 (PVM ...
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bioRxiv - Neuroscience 2024Quote: ... were transduced with in-house prepared viral vectors with a AAV8 serotype construct containing pAAV.CAG.GCaMP6s.WPRE.SV40 plasmids (Addgene, 100844). The cells were transduced at 17 DIV by replacing the cell medium with fresh neuronal medium containing a viral load of 5e2 viruses/cell ...
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bioRxiv - Molecular Biology 2024Quote: ... pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A) was a gift from Feng Zhang (Addgene plasmid # 42335 ...
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bioRxiv - Molecular Biology 2024Quote: ... pAC147-pCR8-dCas9VP64 was a gift from Rudolf Jaenisch (Addgene plasmid # 48219; http://n2t.net/addgene:48219; RRID:Addgene_48219) (Cheng et al ...
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bioRxiv - Neuroscience 2024Quote: ... The same mice also went through a prior surgery three weeks earlier with AAV9.hSyn.DIO.mCherry.WPRE (1.00 X 1013 GC/ml; Addgene #50459) to label OTRCs in mPFC ...
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bioRxiv - Neuroscience 2024Quote: ... 200 nl of lenti pSICO.DIO.shRheb (1.00 X 1013 GC/ml, packaged by Vigene, Addgene #81087) was bilaterally injected into the medial prefrontal cortex [-2.00 mm anterior posterior (AP) ...
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bioRxiv - Neuroscience 2024Quote: ... pCAG-B19G (Addgene #59921), pCAG-B19L (Addgene #59922) ...
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bioRxiv - Neuroscience 2024Quote: ... The resultant AAV plasmids were rAAV-452h-minBG-mTFP1-WPRE3-BGHpA (Addgene plasmid #191708, alias AiP12700) and rAAV-444h-minBG-mTFP1-WPRE3-BGHpA (Addgene plasmid #191729 ...
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bioRxiv - Neuroscience 2024Quote: All shRNA plasmids were made by digesting pLKO.1 hPGK-BFP TRC cloning vector (Addgene #191566) with AgeI-HF and EcoRI-HF and annealed shRNA sequences were ligated with Quick Ligase Buffer (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: Mission plasmids encoding a control scrambled shRNA (Scr) and individual shRNA oligos against Pkm1 and Pkm2 (Supp. Table 1) were cloned in the pLKO_TRC001 vector (Addgene cat no. 10878) as previously described (54) ...
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bioRxiv - Molecular Biology 2024Quote: ... which was a kind gift from Eric Lander & David Sabatini (Addgene plasmid #50661; http://n2t.net/addgene:50661; RRID:Addgene_50661) (Wang et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293T were co-transfected with lenti-vector pMDLg/pRRE (Addgene no. 12251), pRSV-Rev (Addgene no ...
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bioRxiv - Molecular Biology 2024Quote: ... and the lentiviral packaging plasmid pCMV-VSV-G a gift from Bob Weinberg (Addgene plasmid #8454; http://n2t.net/addgene:8454; RRID:Addgene_8454) (Stewart et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentiviral vectors expressed SIX1 (plasmid #120476) and SIX2 (plasmid #120477) with control under an EF1α promoter (Addgene, Watertown, MA).
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bioRxiv - Molecular Biology 2024Quote: ... pX330-U6-Chimeric_BB-CBh-hSpCas9 was a gift from Feng Zhang (Addgene plasmid # 42230; http://n2t.net/addgene:42230; RRID:Addgene_42230). Repeat sequences from pγsat were assembled using the MXS-chaining strategy(Sladitschek and Neveu 2015 ...
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bioRxiv - Molecular Biology 2024Quote: ... pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A) was a gift from Feng Zhang (Addgene plasmid # 42335; http://n2t.net/addgene:42335; RRID:Addgene_42335) (Cong et al ...
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bioRxiv - Molecular Biology 2024Quote: ... pAC147-pCR8-dCas9VP64 was a gift from Rudolf Jaenisch (Addgene plasmid # 48219 ...
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bioRxiv - Molecular Biology 2024Quote: ... which was obtained from Addgene (#52963). Mouse bone marrow c-Kit+ cells from Rosa26-LSL-Cas9 knockin mice were transduced with the sgRNAs using the lentivirus and were selected for stable expression of the sgRNAs using puromycin (1 μg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... pX330-U6-Chimeric_BB-CBh-hSpCas9 was a gift from Feng Zhang (Addgene plasmid # 42230 ...
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bioRxiv - Molecular Biology 2024Quote: ... The pγsat was a gift from Niall Dillon (Addgene plasmid # 39238; http://n2t.net/addgene:39238; RRID:Addgene_39238). The Gapdh random hexamer probe was generated using primers described in Table S1 for qPCR ...
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bioRxiv - Neuroscience 2024Quote: ... The AAV11 capsid gene58 was synthesized and inserted into the pAAV2/1 helper vector (Addgene #112862). The following viruses were used in this study ...
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bioRxiv - Neuroscience 2024Quote: ... and AAV8-CAG-DIO-ChRger2-TS-YFP (Addgene #127239) (packaged by Vector Biolabs and Vigene).
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bioRxiv - Neuroscience 2024Quote: ... pAAV-hSyn-Flex-SypEGFP and pAAV-fDIO-DTR-mCherry were generated from the original vectors (Addgene #55650 ...
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bioRxiv - Neuroscience 2024Quote: ... human GNG2 (Gγ2; Addgene #67018, a gift from Catherine Berlot) and zebrafish voltage-sensitive phosphatase (DrVSP ...
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bioRxiv - Neuroscience 2024Quote: ... For anterograde tracing of PV or SST neurons of the audTRN and control experiments we injected 250nL or 400nL of AAV5-hSyn-DIO-mCherry (full titer, Addgene 50459-AAV5).
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bioRxiv - Neuroscience 2024Quote: ... EnvA-N2C-dG-tdTomato (2×109, Center for Neuroanatomy with Neurotropic Viruses, CNNV)m AAV9-hSyn-DIO-hM4d(Gi)-mCherry (Addgene #44362)32 ...
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bioRxiv - Neuroscience 2024Quote: For soma-targeted inactivation of PV or SST neurons of the audTRN we injected 400nL of AAV5_hSyn1-SIO-stGtACR1-FusionRed (full titer, Addgene 105678-AAV5). For anterograde tracing of PV or SST neurons of the audTRN and control experiments we injected 250nL or 400nL of AAV5-hSyn-DIO-mCherry (full titer ...
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bioRxiv - Neuroscience 2024Quote: ... mScarlet-FRT and mGreenLantern-FRT plasmids were generated by subcloning the mScarlet and mGreenLantern coding sequence (Addgene #85042 and #164468, respectively) inside the FRT-flanking region of a CAG-promoter plasmid (Addgene #99133) ...
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bioRxiv - Neuroscience 2024Quote: ... 107173).58–60 Each was separately inserted into the pZac2.1 GfaABC1D-tdTomato vector109 obtained from Addgene (44332) after removing tdTomato sequence ...
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bioRxiv - Molecular Biology 2024Quote: The optoDroplet construct pHR-mCh-Cry2WT was a gift from Clifford Brangwynne (Addgene plasmid #101221)38 ...
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bioRxiv - Molecular Biology 2024Quote: ... PX458 was a gift from Feng Zhang (Addgene plasmid # 48138) 68 ...
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bioRxiv - Molecular Biology 2024Quote: ... Because the CRISPR-Cas9 vector used in this study is pSpCas9(BB)-2A-GFP (PX458) (#48138, Addgene) with U6 promoter ...
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bioRxiv - Molecular Biology 2024Quote: ... the vector fragment was amplified using PCR from the plasmid p415-GalL-Cas9-CYC1t (Addgene #43804) to keep the GalL promotor and CYC1t terminator but delete the Cas9 sequence which would be replaced by the TAP-tagged protein of interest in the following construction ...