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Citations for Addgene :
3401 - 3450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... eGFP was virally expressed in STN neurons through unilateral injection of AAV9-hSyn-eGFP (RRID:Addgene_50465 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-Syn-flex-PSAM4-GlyR-IRES-EGFP was a gift from Scott Sternson16 (Addgene plasmid # 119741 ...
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bioRxiv - Neuroscience 2024Quote: ... Feng Zhang (Addgene plasmid # 61591). Sanger sequencing confirmed the insertion of the Gabbr1 sgRNA using the primer TAACCACGTGAGGGCCTATTTC.
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bioRxiv - Neuroscience 2024Quote: ... or retrograde serotype AAV-CAG-GFP (gift from Edward Boyden; Addgene viral prep # 37825-AAVrg) were injected via a glass capillary nanoinjector (Neurostar ...
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bioRxiv - Neuroscience 2024Quote: ... we amplified the FLP ORF from pCAG-Flpo (a gift from Dr. Massimo Scanziani; Addgene plasmid # 60662) with a AgeI restriction site on the 5’ end ...
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bioRxiv - Neuroscience 2024Quote: A bicistronic virus expressing both opsin and GCaMP indicator, AAV9-hSyn-DIO-jGCaMP8s-P2A-stChrimsonR(LaFosse et al., 2023) (Addgene, 174007) was diluted in phosphate-buffered saline (final titer ...
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bioRxiv - Neuroscience 2024Quote: ... The CBh promoter was isolated from pX330-U6-Chimeric_BB-CBh-hSpCas9 (a gift from Dr. Feng Zhang; Addgene plasmid # 42230) by digestion of the KpnI and AgeI restriction sites and inserted into the KpnI/AgeI restriction sites of the pAAV-EF1a-F-FLEX-mNaChBac-T2A-tdTomato backbone ...
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bioRxiv - Neuroscience 2024Quote: CBh-eGTACR1-mScarlet was generated by inserting KpnI and AgeI restriction sites flanking EF1a in the pAAV-EF1a-F-FLEX-mNaChBac-T2A-tdTomato (a gift from Dr. Massimo Scanziani; Addgene plasmid # 60658) through pcr cloning ...
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bioRxiv - Neuroscience 2024Quote: ... USA (pAAV5-CaMKIIa-hM4D(Gi)-mCherry and pAAV5-CaMKIIa-hM3D(Gq)-mCherry were gifted from Bryan Roth (Addgene viral prep # 50477and # 50476 ...
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bioRxiv - Neuroscience 2024Quote: ... we isolated mScarlet from pmScarlet_C1 (a gift from Dr. Dorus Gadella; Addgene plasmid # 85042) through pcr amplification ...
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bioRxiv - Neuroscience 2024Quote: ... but now obtained from Addgene, USA (pAAV5-CaMKIIa-hM4D(Gi)-mCherry and pAAV5-CaMKIIa-hM3D(Gq)-mCherry were gifted from Bryan Roth (Addgene viral prep # 50477and # 50476 ...
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bioRxiv - Neuroscience 2024Quote: ... Plasmid containing dLight viral DNA (pAAV-CAG-dLight1.3b) was purchased from Addgene (#125560; Patriarchi et al., 2018) and packaged by the University of Minnesota’s Viral Vector and Cloning Core with a titer of ~1x1013 GC/ml (AAV5-CAG-dLight1.3b) ...
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bioRxiv - Neuroscience 2024Quote: ... constructed by the VVF) and camk2a_hM4D(Gi)_mCherry (4.4*109 pc/ul; Addgene plasmid #50477) were injected into RE ...
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bioRxiv - Neuroscience 2024Quote: ... Channelrhodopsin (ChR2) expressing virus (pAAV9-hSyn-hChR2(H134R)-mCherry) (AddGene, 26976-AAV9, Watertown, MA) and Retrobeads™ (Red RetroBeads ...
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bioRxiv - Neuroscience 2024Quote: ... with PCR of the hDlxI56i enhancer and minimal beta globin promoter from hDlxI56i-minBglobin-iCre-4X2C (Addgene #164450). hDlxI56i eGFP plasmid was made by cutting CMV-eGFP-synapsin (Chi et al. ...
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bioRxiv - Neuroscience 2024Quote: ... All shRNA plasmids were made by digesting pLKO.1 hPGK-BFP TRC cloning vector (Addgene #191566) with AgeI-HF and EcoRI-HF and ligating annealed shRNA sequences with Quick Ligase Buffer (NEB ...
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bioRxiv - Neuroscience 2024Quote: HEK293FT cells were transfected by calcium phosphate with lentiviral constructs along with the associated packaging plasmids psPAX2 (a gift of Didier Trono, Addgene #12260) and pMD2.G (a gift of Didier Trono ...
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bioRxiv - Neuroscience 2024Quote: ... CaMKII Mito4x-jRCaMP1b and hDlxI56i Mito4x-jRCaMP1b were made by cutting CMV-Mito4x-jRCaMP1b (Addgene #127873) with MluI and BamHI and performing PCR of CaMKII and hDlxI56i from CaMKII and hDlxI56i synaptophysin-pHluorin ...
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bioRxiv - Neuroscience 2024Quote: ... hSyn_hM4D(Gi)_mCherry (4.59 × 109 pc/ul; Addgene plasmid #50475); were injected into RE (250 nl ...
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bioRxiv - Neuroscience 2024Quote: ... Experimental mice were injected with AAV5-hsyn-DIO-hM3D(Gq)-mcherry (Addgene #44361).
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bioRxiv - Neuroscience 2024Quote: ... For calcium imaging an AAV was injected (AAV1.Syn.GCaMP6m.WPRE.SV40; RRID: Addgene_100841, titre: 2*1012) into the PPC (from Bregma ...
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bioRxiv - Neuroscience 2024Quote: ... the control group (MC3R-Cre positive mice) was unilaterally injected with AAV5-hsyn-DIO-mcherry (Addgene #50459). Experimental mice were injected with AAV5-hsyn-DIO-hM3D(Gq)-mcherry (Addgene #44361).
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bioRxiv - Neuroscience 2024Quote: ... pAAV-hSyn-mCherry was a gift from Karl Deisseroth (Addgene viral prep # 114472-AAVrg; http://n2t.net/addgene:114472; RRID:Addgene_114472). The needle was allowed to sit in place for 3.0-minutes to restrict backflow through the needle track ...
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bioRxiv - Neuroscience 2024Quote: ... Cas9 expression vector pT3TS-nCas9n (Addgene), linearized with XbaI (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... eNpHR3.0-eYFP was also virally expressed in the STN of vGluT2-Cre mice through unilateral injection of Cre-dependent AAV9-Ef1a-DIO-eNpHR3.0-eYFP (RRID:Addgene_26966 ...
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bioRxiv - Neuroscience 2024Quote: ... eNpHR3.0-eYFP was virally expressed in the STN of WT mice through unilateral injection of AAV9-CaMKIIa-eNpHR3.0-eYFP (RRID:Addgene_26971 ...
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bioRxiv - Neuroscience 2024Quote: pAAV-hSyn Con/Foff hChR2(H134R)-EYFP (Addgene plasmid # 55646 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-hSyn-DIO-hM3D(Gq)-mCherry was a gift from Bryan Roth (Addgene plasmid # 44361 ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-hSyn Coff/Fon hChR2(H134R)-EYFP (Addgene plasmid # 55648 ...
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bioRxiv - Neuroscience 2024Quote: We measured dopamine release using fiber photometry and GRABDA sensors (AddGene #140554). We injected AAV9-hsyn-GRAB DA2h to drive expression of the GRAB sensor ...
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bioRxiv - Neuroscience 2024Quote: Glutamate was monitored in primary astrocytes using the fluorescent reporter for glutamate pAAV.GFAP.iGluSnFR3.v857.GPI (Addgene plasmid # 178338) which was a gift from Kaspar Podgorski (Aggarwal et al. ...
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bioRxiv - Neuroscience 2024Quote: ... The following plasmids were used for transfections: EGFP-Rubicon (RRID: Addgene 221657), LAMP1-Halo (RRID ...
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bioRxiv - Neuroscience 2024Quote: ... with 1µl of AAV9-CaMKII-GCaMP6f (Addgene, #100834-AAV9, dilution 1/15) at 0.75µl.min−1 (microinjection pump ...
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bioRxiv - Neuroscience 2024Quote: ... HeLa cells were transfected with 1.5 µg of untagged human Parkin (RRID: Addgene 187897) using Lipofectamine 2000 for 18-24 hrs prior to treatment.
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bioRxiv - Neuroscience 2024Quote: ... mCherry-RubiconCGHL (RRID: Addgene 221659) and EGFP-Rab7A (RRID ...
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bioRxiv - Neuroscience 2024Quote: ... LAMP1-Halo (RRID: Addgene 221655), mCherry-LC3 (RRID ...
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bioRxiv - Neuroscience 2024Quote: ... Targeting of the transgene cassettes into an endogenous gene locus was accomplished via CRISPR/Cas9-mediated genome editing using circularized targeting vector in combination with a gene-specific guide vector (Addgene plasmid #42230). The129S6B6F1 ES cell line ...
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bioRxiv - Neuroscience 2024Quote: The following AAV vectors were purchased from Addgene (Watertown, MA, USA): AAV9-hSyn-DIO-hM4D(Gi)-mCherry (#44362) ...
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bioRxiv - Neuroscience 2024Quote: ... packaging plasmid and the pMD2.G (Addgene plasmid #12259) envelope plasmid ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293T cells were triple transfected with lentivirus packaging plasmid psPAX2 and the envelope plasmid pCMV-VSV-G (Addgene plasmids #12260 and #8454) in addition to the transgene of interest using FuGene HD (Promega) ...
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bioRxiv - Neuroscience 2024Quote: ... or AAV9-synP::DIO-EGFP (Addgene 100043-AAV9) were injected at 3×109 genome copies (0.25 µL ...
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bioRxiv - Neuroscience 2024Quote: ... Both plasmid expression vectors (ADDGENE) were modified with 3’ and 5’ UTR sequences (plasmids constructed by GeneScript ...
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bioRxiv - Neuroscience 2024Quote: ... as well as AAV1-CB7-CI-mCherry-WPRE-RBG (AddGene #105544) to drive the expression of mCherry to correct for motion artificats ...
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bioRxiv - Neuroscience 2024Quote: ... and then bilaterally injected with 150 nl of rgAAV-hSyn-DIO-hM3Gq-mCherry (∼8 x 1012 GC/mL, cat. #: 44361-AAVrg, Addgene) in the suprachiasmatic nucleus (SCN ...
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bioRxiv - Neuroscience 2024Quote: ... Ryan (Weill Cornell Medical College, New York, Addgene #187896). ATG9-pH was a gift from Dr ...
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bioRxiv - Neuroscience 2024Quote: ... Each eye was intravitreally injected with 1 μL of AAV2-hSyn-DIO-mCherry (∼8 x 1012 GC/mL, cat. #: 50459-AAV2, Addgene) using a custom Hamilton syringe (Borghuis Instruments ...
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bioRxiv - Neuroscience 2024Quote: ... Plasmid #116869)27 using HindIII and pAAV-Ef1a-DIO-Sun1GFP-WPRE-pA (Addgene, Plasmid #160141)106 using AscI ...
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bioRxiv - Neuroscience 2024Quote: ... we used CamKII-LGI1-pH (Addgene Plasmid #185537) to replace LGI1 by rat NPTX1 (a gift from Dr ...
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bioRxiv - Neuroscience 2024Quote: ... and FSW-TM-HRP (Addgene #82540). The DNA was amplified using the PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-hSyn-mCherry was a gift from Karl Deisseroth (Addgene viral prep # 114472-AAVrg ...