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Citations for Addgene :
3151 - 3200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... coli expression assembled into pCDB179 (Addgene, plasmid number #91960) by Gibson assembly ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... lentiGuide-Puro vector (a gift from Feng Zhang; Addgene_52963). Cell lines stably expressing dCas9-KRAB or dCas9-P300 were transfected with plasmids encoding gRNAs or lentiGuide empty vector using TransIT-X2 (Mirus Bio) ...
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bioRxiv - Molecular Biology 2024Quote: ... Laccase2-MCS-Exon-Vector was a gift from Jeremy Wilusz (Addgene plasmid # 69893; http://n2t.net/addgene:69893 ; RRID:Addgene_69893). For overexpression of circHIPK3 followed by IGF2BP2 immunofluorescence ...
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bioRxiv - Neuroscience 2024Quote: ... we made a unilateral injection of 600nl CAMKII-GCaMP6f (Addgene, 100834-AAV1) in the left hemisphere using the same coordinates ...
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bioRxiv - Neuroscience 2024Quote: ... we made bilateral injections of 200-400 nl of hSyn-hM4Di-tdTomato (Addgene, 50475-AAV2) into the BLA (AP:+/-3.30 ...
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bioRxiv - Neuroscience 2024Quote: ... animals were anesthetized with isoflurane and injected with jGCaMP8m (rAAV1-syn-GCaMP8m-WPRE, Addgene: 162375-AAV1) in dorsal CA1 (AP ...
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bioRxiv - Molecular Biology 2024Quote: ... Baculoviruses were generated using pLIB-based constructs (Addgene, 80610). 500 ml Sf9 cells (1×106/ml ...
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bioRxiv - Microbiology 2024Quote: ... The HindIII-linearized p1NIL plasmid (Addgene plasmid number 20187) was ligated with the HindIII-digested PCR product ...
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bioRxiv - Cell Biology 2024Quote: ... Qing Zhong’s plasmid (Addgene#280227(68)) and TurboID from Dr ...
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bioRxiv - Cell Biology 2024Quote: ... with the HA::SQSTM1/p62 plasmid as template (Addgene#280227). We verified the sequences of all lentiviral constructs using Sanger sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... and VSV-G (Addgene#8454) at a ratio of 1:1:3 ...
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bioRxiv - Cell Biology 2024Quote: ... psPAX2 (Addgene#12260), and VSV-G (Addgene#8454 ...
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bioRxiv - Cell Biology 2024Quote: ... hrGFP-KEAP1 (Addgene #28025) and HA-SQSTM1 (Addgene #280227 ...
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bioRxiv - Cell Biology 2024Quote: ... For HA-LC3B-Myc (Addgene #137757), hrGFP-KEAP1 (Addgene #28025 ...
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bioRxiv - Developmental Biology 2024Quote: ... HA- human HA-Ubiquitin was a gift from Edward Yeh (Addgene plasmid # 18712; Kamitani et al., 1997)
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bioRxiv - Developmental Biology 2024Quote: ... of Fbxo24 and Skp1 were cloned and amplified using cDNA obtained from mouse testis and inserted into the multiple cloning site of pCAG1.1 vector (Addgene; Plasmid #173685). To generate the expression vector coding Fbxo24(ΔF-box) ...
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bioRxiv - Immunology 2024Quote: ... cells were co-transfected with pcDNA-mGATA3 (#1332, Addgene) and MSCV-mGfi1-IRES-GFP expression plasmids ...
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bioRxiv - Immunology 2024Quote: ... was co-transfected with the lentiviral packaging plasmids pRSV-Rev (Addgene plasmd #12253), pMDLg/pRRE (Addgene plasmd #12251 ...
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bioRxiv - Biophysics 2024Quote: ... pHAGE-RSV-GCaMP6s (Addgene 80146, a gift from Darrell Kotton) plasmids were used for lentiviral cell transduction ...
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bioRxiv - Biophysics 2024Quote: pEGFP-C1 F-tractin-EGFP (Addgene 58473, a gift from Dyche Mullins) plasmids were used for transient transfections ...
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bioRxiv - Biophysics 2024Quote: ... the pLKO.1 puro (Addgene; 8453; a gift from Bob Weinberg) backbone was used ...
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bioRxiv - Cancer Biology 2024Quote: ... The following guide sequences were cloned in the lentiCRISPRV2 vector that was a gift from Feng Zhang (Addgene plasmid #52961): sg BCL-xL ...
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bioRxiv - Cell Biology 2024Quote: ... the pLVX-KRT6A-mRb-IRES-Neo construct includes an mRuby2 (Addgene #40260) 99 sequence in-frame with the KRT6A sequence separated by the same linker.
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bioRxiv - Cell Biology 2024Quote: Keratin sequences were cloned by polymerase chain reaction (PCR) from pBabe-RFP1-KRT5-hygro (Addgene #58493) and pDONR22-KRT6A (DNASU clone HsCD00039474 ...
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bioRxiv - Cell Biology 2024Quote: ... the pAdDeltaF6 plasmid (Addgene #112867), and the pAAV2/8 plasmid (Addgene # 112864 ...
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bioRxiv - Cell Biology 2024Quote: ... and the pAAV2/8 plasmid (Addgene # 112864) using polyethylenimine (1 µg/µl ...
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bioRxiv - Genetics 2024Quote: ... we inserted the resulting ddCas12a-[Repr] open reading frame in-frame with P2A-BFP in a piggyBac vector (Addgene #133568) to enable direct comparison with other fusion protein constructs cloned in the same vector backbone (crRNA’s are encoded on separate plasmids as described below).
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bioRxiv - Genetics 2024Quote: ... We generated SiT-ddCas12a-[Repr] by introducing the DNase-inactivating E993A by PCR-based mutagenesis using SiT-Cas12a-[Repr] (Addgene #133568) as template ...
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bioRxiv - Developmental Biology 2024Quote: ... Microtubules in krt4+ keratinocytes and cytonemes were labelled using subclones from Tuba1-mCherry [(Addgene #49149] (Friedman et al., 2010).
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bioRxiv - Developmental Biology 2024Quote: ... a clone of EUC313f02 NipblFLEX/+ ES cells (European Conditional Mouse Mutagenesis Program) was transfected with pCAG-Cre:GFP (Addgene #13776) using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.85 µg pMD2G (Addgene; 122259) using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... full length Rab3A sequence was amplified from EGFP-Rab3A vector (Addgene plasmid # 49542) using PCR ...
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bioRxiv - Neuroscience 2024Quote: ... The EB3-Tdtomato construct was obtained from Addgene (Addgene plasmid #50708; http://n2t.net/addgene:50708; RRID:Addgene_50708). For rAAV9-syn-EGFP-Rab3A construct ...
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bioRxiv - Neuroscience 2024Quote: ... The EB3-Tdtomato construct was obtained from Addgene (Addgene plasmid #50708 ...
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bioRxiv - Neuroscience 2024Quote: ... were cloned by PCR amplification of human LGals3 and LGals8 cDNAs from the pHAGE-mKeima-LGALS3 and pHAGE-FLAG-APEX2-LGALS8 plasmids (Addgene plasmids #175780 and #175758 ...
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bioRxiv - Neuroscience 2024Quote: ... 6-week-old Slc17a7Cre mice were injected with pAAV-EF1a-DIO-tdTomato-WPRE virus (RRID:Addgene_133786 ...
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bioRxiv - Neuroscience 2024Quote: ... an oligonucleotide pair (Supplemental Table 3) was annealed and cloned into BbsI-digested pCFD3-dU6-3gRNA (Addgene #49410) as described (Port et al. ...
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bioRxiv - Neuroscience 2024Quote: ... oligonucleotide pairs (Supplemental Table 4) were used for PCR and inserted into pCFD5 (Addgene #73914) via Gibson Assembly ...
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bioRxiv - Neuroscience 2024Quote: ... sechellia UAS-SPARC2-D-CsChrimson: we digested a SPARC2-backbone vector (Addgene #133562) with SalI and inserted a CsChrimson-Venus cassette after PCR amplification from pBac(UAS-ChR2 CsChrimson,3xP3::dsRed ...
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bioRxiv - Neuroscience 2024Quote: ... sechellia nos-Cas9 (Auer et al., 2020) or co-injected with pHsp70-Cas9 (400 ng μl−1) (Addgene #45945; for D. simulans transgenesis) (Gratz et al. ...
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bioRxiv - Neuroscience 2024Quote: ... The Dsec-nSyb-ΦC31 line was generated by integration of nSyb-ΦC31 (Addgene #133868) into Dsec-attP26 (see next section).
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bioRxiv - Neuroscience 2024Quote: ... and a 2nd generation viral packaging system comprising gag/pol/tat/rev (psPAX2, Addgene, #12260) and VSV-G (pMD2.G ...
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bioRxiv - Neuroscience 2024Quote: ... and VSV-G (pMD2.G, Addgene, #12259). Cells were seeded in a 15 cm dish in 22.5 ml medium (DMEM with 4.5g/l glucose and pyruvate ...
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bioRxiv - Neuroscience 2024Quote: ... conferring resistance to puromycin (Addgene 52961). After cell transduction with the lentiviruses expressing the Cas9/gRNAs ...
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bioRxiv - Neuroscience 2024Quote: ... gRNAs were designed using the prediction software https://portals.broadinstitute.org/gpp/public/analysis-tools/sgrna-design and inserted in pX330-U6-Chimeric_BB-Cbh-hSpCas9 (gift from Feng Zhang, Addgene plasmid #42230 ...
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bioRxiv - Neuroscience 2024Quote: ... These mice were unilaterally injected with AAV2-EF1a-DIO-ChR2-eYFP (obtained from Addgene) in the left prefrontal cortex based on stereotaxic coordinates measured from bregma (AP 1.8mm ...
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bioRxiv - Neuroscience 2024Quote: ... and AAVrg-EF1a-Cre-mCherry (obtained from Addgene) was injected in the downstream target region based on stereotaxic coordinates measured from bregma (NAc ...
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bioRxiv - Neuroscience 2024Quote: ... thirteen F1 offspring were bilaterally injected with AAV2-hSyn-DIO-GqDREADD (obtained from Addgene) based on stereotaxic coordinates measured from bregma (AP -1.5mm ...
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bioRxiv - Neuroscience 2024Quote: ... AAV.9.FLEX.rev.ChR2(H134R).mCherry (optogenetics, 140 nl, Addgene, no. 18916), AAV.9.Ef1A.fDIO.EYFP (control for optogenetics ...
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bioRxiv - Neuroscience 2024Quote: ... AAV.9.Ef1A.fDIO.EYFP (control for optogenetics, 140 nl, Addgene, no55641).