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Citations for Addgene :
3201 - 3250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... stGtACR2-expressing adeno-associated virus (AAV) vector (pAAV_hSyn1-SIO-stGtACR2-FusionRed, Addgene, #105677) stock solution was diluted to 5×1011 gc/ml titers ...
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bioRxiv - Molecular Biology 2024Quote: ... CMV promotor and tdTomato sequences were obtained from pMuLE ENTR CMV tdTomato L5-L2(43) (Addgene #62152), and CMV promotor for EGFP was obtained from pMuLE ENTR CMV eGFP L3-L2(43 ...
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bioRxiv - Molecular Biology 2024Quote: ... pUCmini-iCAP-PHP.eB(44) (Addgene #103005), pAdDeltaF6 (Addgene #112867) ...
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bioRxiv - Molecular Biology 2024Quote: ... pAdDeltaF6 (Addgene #112867), into HEK293T cell ...
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bioRxiv - Molecular Biology 2024Quote: ... and CMV promotor for EGFP was obtained from pMuLE ENTR CMV eGFP L3-L2(43) (Addgene #62143) by PCR amplification ...
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bioRxiv - Molecular Biology 2024Quote: ... All fragments were inserted into AAV backbone vector (pAAV-CAG-GFP, Addgene #37825) using NEB HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... and cloned into pU6-tevopreq1-GG-acceptor41 (a gift from David Liu; Addgene 174038). K562 cells stably expressing the mSc-TOSI reporter were transfected with PE3max-epegRNA (tevopreQ1 ...
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bioRxiv - Cancer Biology 2024Quote: ... and pU6-tevopreq1-GG-acceptor41 (a gift from David Liu; Addgene 174038). To repurpose the mTORC1 signaling reporter mVenus-TOSI previously developed for mouse40 ...
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bioRxiv - Cancer Biology 2024Quote: ... packaging plasmids psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Neuroscience 2024Quote: ... BMECs were transfected with pAAV-CAG-GCaMP6f (Addgene). 48 hours later ...
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bioRxiv - Neuroscience 2024Quote: ... or pAAV-CAG-tdTomato (Addgene). To evaluate the connectivity of miBrain-neurons ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs containing tetON Ngn2 were transfected with a plasmid driving GCaMP3 expression under the CAG promoter (Addgene #62810), then differentiated into neurons and incorporated into miBrain cultures as described above ...
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bioRxiv - Neuroscience 2024Quote: Neurons were isolated by first virally labeling neurons from the same batch with pAAV-CAG-tdTomato for 24 hours (1:50 dilution, Addgene 59462-AAV1) prior to incorporation into miBrain or monoculture ...
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bioRxiv - Neuroscience 2024Quote: ... A stably expressing mCherry cell line was utilized for live imaging endothelial cells that was previously developed using pAAVS1-P-CAG-mCh (Addgene). FluoroMyelin dye was used for live labeling myelin in miBrains (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... Astrocytes were labeled for live assays prior to incorporation into miBrains using an AAV (pAAV-CAG-tdTomato, Addgene or AAV9-CAG-GFP ...
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bioRxiv - Biophysics 2024Quote: ... which was a gift from Emanuel Strehler (Addgene plasmid # 47598) (Rhyner et al ...
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bioRxiv - Plant Biology 2024Quote: All libraries used in this study were constructed using pPSup (Addgene no ...
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bioRxiv - Plant Biology 2024Quote: ... and rbcS-E9 enhancers and their 169-bp 3′ and 5′ segments as well as the 35S enhancer were PCR amplified from pZS*11_4enh (Addgene no ...
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bioRxiv - Plant Biology 2024Quote: ... The luciferase coding sequence was amplified from pGreen_dualluc_3’UTR_sensor (Addgene no ...
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bioRxiv - Physiology 2024Quote: pAAV-mCherry-FLEX-dtA (Addgene, 58536) was subcloned and packaged in an AAV serotype retrograde (Applied Biological Materials ...
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bioRxiv - Physiology 2024Quote: Circadian clock gene activity was recorded using the vectors pLV6-Bmal-luc (Addgene plasmid #68833) and pLV6-Per2-luc ...
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bioRxiv - Physiology 2024Quote: ... psPAX2 (#12260, Addgene), and the two transfer vectors ...
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bioRxiv - Cell Biology 2024Quote: ... gRNAs were sub-cloned into vector pSpCas9(BB)-2A-Puro (PX459) (Addgene, 48139, deposited by the F. Zhang lab), and gRNA plasmids for targeting PRKAA1 and PRKAA2 were co-transfected into cells with TransIT-LT1 (Mirus ...
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bioRxiv - Cell Biology 2024Quote: ... a CRISPRi construct (pLX_311-KRAB-dCas9, gift from John Doench & William Hahn & David Root, Addgene plasmid # 96918 ...
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bioRxiv - Cell Biology 2024Quote: ... a CRISPRi construct (pLX_311-KRAB-dCas9, gift from John Doench & William Hahn & David Root, Addgene plasmid # 96918; http://n2t.net/addgene:96918; RRID:Addgene_96918) was lentivirally introduced into the A549s expressing inducible-XBP1s ...
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bioRxiv - Microbiology 2024Quote: ... and pMD2.G (Addgene, #12259) and psPAX2 (Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... had successful transfection of PiCo neurons by using a pAAV-hSyn Con/Fon hChR2(H134R)- EYFP adenovirus vector (cat# 55645-AAV8; AddGene, USA ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-hSyn-hChR2(H134R)-EYFP was a gift from Karl Deisseroth (Addgene plasmid # 26973 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-hSyn-hChR2(H134R)-EYFP was a gift from Karl Deisseroth (Addgene plasmid # 26973; http://n2t.net/addgene:26973; RRID:Addgene 26973).
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bioRxiv - Cancer Biology 2024Quote: ... HA-ubiquitin plasmid (#18712) was purchased from Addgene. P3-Flag-CMV-10-ZEB1 (PVTB00266-2b ...
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bioRxiv - Developmental Biology 2024Quote: ... the Tol2-bActin:secA-YFP plasmid (Addgene #105664, a gift from Dr. Qing Deng) was co injected with Tol2 transposase mRNA as previously described (Suster et al. ...
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bioRxiv - Biochemistry 2024Quote: ... The construct for LbCas12a expression (pMAL-His-LbCpf1-EC) - a gift from Jin-Soo Kim (RRID: Addgene 79008) (Kim et al. ...
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bioRxiv - Biochemistry 2024Quote: ... pCMV5 mouse Src was a gift from Joan Brugge & Peter Howley (Addgene plasmid # 13663; http://n2t.net/addgene:13663 ; RRID:Addgene_13663). The mouse Src gene was subcloned into the pEGN Bacmam vector(45) ...
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bioRxiv - Cell Biology 2024Quote: ... Rab7a with a point mutation at residue 8 from leucine to alanine (L8A) was cloned into pEmerald-C1 (Addgene#54734, Davidson Lab) at XhoI-BamHI sites by Genscript ...
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bioRxiv - Biochemistry 2024Quote: ... pCMV5 mouse Src was a gift from Joan Brugge & Peter Howley (Addgene plasmid # 13663 ...
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bioRxiv - Biochemistry 2024Quote: ... Ube1 was a gift from Cynthia Wolberger 35(Addgene plasmid # 34965).
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bioRxiv - Cell Biology 2024Quote: ... and the other gRNA was cloned into the px330-mCherry plasmid (modified from Addgene; Cat #98750 to incorporate mCherry reporter) ...
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bioRxiv - Cell Biology 2024Quote: ... The CRY2olig cDNA was prepared through site-directed mutagenesis (E409G) of CRY2 gene that was obtained from Addgene. The PCR products were subcloned into pcDNA3.1 myc/His ver ...
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bioRxiv - Cancer Biology 2024Quote: ... Guide RNAs were cloned into pX330-U6-Chimeric_BB-CBh-hSpCas9 (a gift from Feng Zhang; Addgene 42230) and their sequences are provided in the Supplementary material section ...
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bioRxiv - Microbiology 2024Quote: ... such that gene could be inserted by Gibson Assembly into p15TV-L (Addgene #26093) linearized by BseRI digestion to produce the expression vector p15TVL-AcdA ...
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bioRxiv - Molecular Biology 2024Quote: ... guide RNAs (gRNAs) against each gene were cloned into pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP (Addgene, #62348) modified to express human CD2 or Thy1.1 as cell surface markers ...
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bioRxiv - Plant Biology 2024Quote: ... pICH41308 (Addgene plasmid #47998) using Golden Gate Cloning (Weber et al ...
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bioRxiv - Molecular Biology 2024Quote: psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260 ; http://n2t.net/addgene:12260 ; RRID:Addgene_12260)
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bioRxiv - Molecular Biology 2024Quote: psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260 ...
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bioRxiv - Bioengineering 2024Quote: ... and envelope (pMD2.G (Addgene plasmid, #12259) plasmids ...
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bioRxiv - Cell Biology 2024Quote: ... GFP-Rab7T22N (Addgene #12660, Pagano Lab), pCAG-Cre-GFP (Addgene #13776 ...
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bioRxiv - Neuroscience 2024Quote: ... pCAG-Brn-DBD-EnR (plasmid #19714, Addgene) and pCAG-Brn2 (plasmid #19711 ...
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bioRxiv - Neuroscience 2024Quote: Adeno-associated virus AAV5.CamKII.GCaMP6f.WPRE.SV40 (Addgene # 100834 ...
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bioRxiv - Neuroscience 2024Quote: ... and the pMD2.G envelope plasmid (Addgene #12259) using Lipofectamine 2000 (ThermoFisher #11668019) ...
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bioRxiv - Neuroscience 2024Quote: ... 6 × 107 RPE1 cells were infected with the human sgRNA library (Human GeCKO v2 Library Cat#1000000048, Addgene) at an MOI of ∼0.3 ...