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Citations for Addgene :
51 - 100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: PancACe and cpGFP were subcloned from the pET28 plasmid into the pLJM1 plasmid (Addgene #19319), and the localization tags were added via primers.
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bioRxiv - Cell Biology 2024Quote: ... pCW-Tet-Cas9-BFP was prepared by Gibson assembly from pCW-Cas9 (Addgene #50661).
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bioRxiv - Cell Biology 2024Quote: ... DNA fragments containing MAP3K1 (MEKK1) or kinase-dead MAP3K1 were excised from pCDNA-MEKK1 plasmids (Addgene #12181 and #12180 ...
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bioRxiv - Cancer Biology 2024Quote: ... containing the viral packaging system of second generation as well as the lentiviral construct of interest pCDH-EF1a-mCherry-EGFP-LC3B (RRID:Addgene_170446), respectively ...
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bioRxiv - Cell Biology 2024Quote: To construct the ER-GCaMP fused to mRuby3, mRuby3 was PCR amplified from the pKanCMV-mRuby3-18aa-actin (Bajar et al., 2016) (Addgene #74255). CMV ER-GCaMP6-210 was restriction digested with AgeI-HF and combined with the PCR amplified mRuby3 via ligation ...
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bioRxiv - Cell Biology 2024Quote: ... The original TSMod was derived from pcDNA3.1-TSMod (Addgene Plasmid #26021)30 ...
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bioRxiv - Cell Biology 2024Quote: ... F-tractin and Linker 1 were derived from pEGFP-C1 F-tractin-EGFP (Addgene Plasmid #5847)48 ...
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bioRxiv - Cell Biology 2024Quote: ... mCherry-FokI and the catalytically dead mCherry-FokI (D540A) cDNAs were subcloned into the mCherry-LacI vector (Addgene plasmid #18985) replacing mCherry ...
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bioRxiv - Neuroscience 2024Quote: ... that was first PCR amplified using custom primers (see Table 2) from a plasmid containing lexAop2 (lexAop2-myr-4xSNAPf, RRID: Addgene 87638) and then restriction digested using HindIII and PspXI (New England BioLabs ...
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bioRxiv - Neuroscience 2024Quote: ... and pCMVR8.74 (Addgene plasmid #22036, RRID:Addgene_22036) were gifts from Didier Trono ...
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bioRxiv - Cell Biology 2024Quote: ... The hDLXI56i enhancer was originally obtained from CN1851-rAAV-hI56i-minBglobin-iCre-4×2C-WPRE3-BGHpA (Graybuck et al., 2021) (Addgene #164450).
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bioRxiv - Cell Biology 2024Quote: ... or pKHH030 (Addgene #89358, gift from Michael Bassik). For pMCB320 ...
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bioRxiv - Cell Biology 2024Quote: CRISPR sgRNAs were cloned either into pMCB320 (Addgene #89359, gift from Michael Bassik) or pKHH030 (Addgene #89358 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV.CamKII(1.3).eYFP.WPRE.hGH was a gift from Karl Deisseroth (Addgene plasmid# 105622 ...
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bioRxiv - Neuroscience 2024Quote: ... Wilson (Addgene viral prep # 105558-AAV9; http://n2t.net/addgene:105558; RRID:Addgene_105558), pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40 was a gift from Douglas Kim & GENIE Project (Addgene viral prep # 100852-AAV1 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40 was a gift from Douglas Kim & GENIE Project (Addgene viral prep # 100852-AAV1 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV.CamKII(1.3).eYFP.WPRE.hGH was a gift from Karl Deisseroth (Addgene plasmid# 105622; http://n2t.net/addgene:105622; RRID:Addgene_105622).
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bioRxiv - Neuroscience 2024Quote: ... pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40 was a gift from Douglas Kim & GENIE Project (Addgene viral prep # 100852-AAV1; http://n2t.net/addgene:100852; RRID:Addgene_100852;)77 ...
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bioRxiv - Neuroscience 2024Quote: ... Wilson (Addgene viral prep # 105558-AAV9 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV.Syn.GCaMP6f.WPRE.SV40 was a gift from Douglas Kim & GENIE Project (Addgene viral prep # 100837-AAV9 ...
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bioRxiv - Cancer Biology 2024Quote: Plasmid expressing constitutively activate RhoA was ordered from Addgene (pSLIK CA RhoA, Plasmid #112894) (MacKay and Kumar ...
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bioRxiv - Cancer Biology 2024Quote: ... psPAX2 and pMD2.G were used (Addgene # 12260 and Addgene # 12259); plasmids were a gift from D ...
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bioRxiv - Cancer Biology 2024Quote: ... psPAX2 and pMD2.G were used (Addgene # 12260 and Addgene # 12259); plasmids were a gift from D ...
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bioRxiv - Cancer Biology 2024Quote: A Cas9-expressing lentivirus stock was produced by transfecting pKLV2-EF1a-Cas9Bsd-W (Addgene #68343) into HEK293T cells together with packaging vectors (psPax2 and pMD2.G) ...
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bioRxiv - Cell Biology 2024Quote: ... pMD2.G (Addgene, #12259) and pLV-tetO-Tfap2c (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... pSpCas9(BB)-2A-PuroR (px459) V2.0 (Addgene, #62988), psPAX2 (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers 11 bearing terminal NotI restriction sites were cloned into PCR4-Shh::lacZ-H11 (Addgene, # 139098). Plasmid clones were validated by Sanger sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... and pLV-tetO-Tfap2c (Addgene, #70269) were obtained from Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... or pBabe-puro-Empty (Addgene, #1764) retroviral constructs along with pCL-10A1 retrovirus packaging vector (Novus Biologicals ...
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bioRxiv - Cancer Biology 2024Quote: ... Whole cell lysates (200 µg) were incubated with GST-RAF-RBD (Addgene) bound to glutathione agarose beads at 4°C for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... cell line was transfected with either pBabe-puro-HA-KRASG12C (Addgene, #58901) or pBabe-puro-Empty (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... or lentiCRISPRV2-Neo (Addgene #98292 where the puromycin resistance gene was replaced with the G418 resistance gene ...
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bioRxiv - Cancer Biology 2024Quote: ... and pMD2.G (Addgene #12259) in a ratio of 4:3:1 ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-PTRE-tight-hM3Dg-mCherry(35) (a gift from William Wisden [Addgene plasmid # 66795 ...
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bioRxiv - Neuroscience 2024Quote: ... This vector was generated using the plasmids pPRIME-CMV-GFP-FF3(34) (a gift from Stephen Elledge (Addgene plasmid # 11663; http://n2t.net/addgene:11663; RRID:Addgene_11663)) and pAAV-PTRE-tight-hM3Dg-mCherry(35 ...
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bioRxiv - Neuroscience 2024Quote: ... This vector was generated using the plasmids pPRIME-CMV-GFP-FF3(34) (a gift from Stephen Elledge (Addgene plasmid # 11663 ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-PTRE-tight-hM3Dg-mCherry(35) (a gift from William Wisden [Addgene plasmid # 66795; http://n2t.net/addgene:66795; RRID:Addgene_66795]). Specifically ...
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bioRxiv - Cell Biology 2024Quote: ... Switzerland (Addgene plasmids 12259 and 12260). HEK293FT culture medium was changed 24 h after transfection ...
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bioRxiv - Cell Biology 2024Quote: ... into pLenti CMV Hygro DEST (Addgene 17454). We generated stable cells lines using these constructs via lentiviral delivery and antibiotic selection ...
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bioRxiv - Cell Biology 2024Quote: ... were cloned into the plasmid TVBB C-term-mScarlet (Addgene 169219) with the purpose of knocking in mScarlet in frame with D882 ...
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bioRxiv - Cell Biology 2024Quote: ... This gRNA was cloned into a Cas9 D10A backbone (PX462) (Addgene 62987). The upstream and downstream homology arms ...
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bioRxiv - Cell Biology 2024Quote: ... Constructs to KD Slug in SGEF KD were cloned into pLKO.1-Hygro (Addgene 24150). To generate Scribble and Dlg1 CRISPR/Cas9 KO cell lines we used pLentiCRISPR v2-Blast (Addgene 83480) ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 1.2 µL of virus (pAAV5-Syn-FLEX-Chrimson-tdTomato, Addgene) was infused unilaterally into the VTA (-5.6 AP ...
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bioRxiv - Cell Biology 2024Quote: ... To generate Scribble and Dlg1 CRISPR/Cas9 KO cell lines we used pLentiCRISPR v2-Blast (Addgene 83480). DH-PH and VSV-G-DH-PH Rescue constructs were cloned using gateway recombination (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... We next used the human EZH2 primary protein sequence available in the addgene for hEZH2 plasmid (#24230, Addgene) and predicted the possible cysteine residues using the GPS-SNO prediction tools ...
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bioRxiv - Cell Biology 2024Quote: ... The two sgRNAs were cloned into either the pSpCas9(BB)-2A-GFP (PX458) plasmids (Addgene, Cambridge, USA)31 ...
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bioRxiv - Cell Biology 2024Quote: ... The gRNA oligos were annealed and inserted into the CasRx vector (Addgene: # 134842) linearized by BsmBI (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... and cloned using a BbsI restriction enzyme digested vector according to the method mentioned by the Feng Zhang lab at the MIT in Addgene (Addgene #62988)42 ...
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bioRxiv - Cell Biology 2024Quote: ... Dyche Mullins at UCSF (Addgene plasmid # 58473) and MDA-MB-231 cells were a gift from Dr ...
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bioRxiv - Molecular Biology 2024Quote: The 2C::tdTomato reporter cell line was generated by transfection of EB3 mESCs (catalog no. AES0139, RIKEN BRC Cell Bank) with a linearized 2C::tdTomato reporter plasmid (catalog no. 40281, Addgene). After 48 h ...