Labshake search
Citations for Addgene :
2601 - 2650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2024Quote: ... (71) and obtained from Addgene. The subcellular markers were expressed under control of ubiquitin promoter and were tagged with mCherry ...
-
bioRxiv - Plant Biology 2024Quote: ... The qRNA cassette was custom-synthesised by GenScript (www.genscript.com) and inserted into into SunTag dCas9 plasmid (Addgene Plasmid #117168) using the KpnI and MauBI restriction enzymes (Thermo Scientific™ ...
-
bioRxiv - Neuroscience 2024Quote: ... the FLEX [IRES-GFP] sequence was introduced into the EcoRV-KpnI sites of pAAV-EF1a-FLEX-GTB (Addgene #26197) using infusion cloning (Takara Bio) ...
-
bioRxiv - Immunology 2024Quote: ... The SEMA7A and MTRAP plasmids were a kind gift from Gavin Wright48 (Addgene plasmid #73115 and #47746). Plasmid #73115 was digested with NotI and EcoRI to excise the SEMA7A sequence ...
-
bioRxiv - Developmental Biology 2024Quote: ... cloned into pUC18 vector (Addgene plasmid #50004) with FRT sites added at both ends of the whole construct.
-
bioRxiv - Developmental Biology 2024Quote: ... cloned into pUC18 vector (Addgene plasmid #50004) with FRT sites added at both ends of the whole construct.
-
bioRxiv - Developmental Biology 2024Quote: ... The CDK1 FRET sensor was a gift from Jonathon Pines (Addgene plasmid # 26064). They were subcloned in the pCDNA 3.1 vector containing a T7 promoter and the constancy was confirmed via DNA sequencing ...
-
bioRxiv - Developmental Biology 2024Quote: ... bovine growth hormon poly(A) signal (Addgene #132775) cloned into pUC18 vector (Addgene plasmid #50004 ...
-
bioRxiv - Developmental Biology 2024Quote: ... cloned into pT2 HB (Addgene plasmid #26557).
-
bioRxiv - Developmental Biology 2024Quote: ... bovine growth hormon poly(A) signal (Addgene #132775) cloned into pUC18 vector (Addgene plasmid #50004 ...
-
bioRxiv - Developmental Biology 2024Quote: ... bovine growth hormon poly(A) signal (Addgene #132775) cloned into pUC18 vector (Addgene plasmid #50004 ...
-
bioRxiv - Developmental Biology 2024Quote: ... cloned into pUC18 vector (Addgene plasmid #50004) with FRT sites added at both ends of the whole construct.
-
bioRxiv - Developmental Biology 2024Quote: ... Fragments were assembled into plasmid pKHR4 (Hoshijima et al, 2016) (Addgene, 74592, Grunwald lab) by Gibson cloning to obtain the final repair template ...
-
bioRxiv - Developmental Biology 2024Quote: ... the Tol2Kit plasmids #327 (p5E-UAS) and #302 (p3E-polyA) (Kwan et al, 2007) and the destination vector pDestTol2pA2CrymCherry (Addgene, 64023 ...
-
bioRxiv - Developmental Biology 2024Quote: ... cloned into pUC18 vector (Addgene plasmid #50004) with FRT sites added at both ends of the whole construct.
-
bioRxiv - Developmental Biology 2024Quote: ... bovine growth hormon poly(A) signal (Addgene #132775) cloned into pUC18 vector (Addgene plasmid #50004 ...
-
bioRxiv - Developmental Biology 2024Quote: ... cloned into pUC18 vector (Addgene plasmid #50004) with FRT sites added at both ends of the whole construct.
-
bioRxiv - Developmental Biology 2024Quote: ... bovine growth hormone poly(A) signal (Addgene #132775) cloned into pUC18 vector (Addgene plasmid #50004 ...
-
bioRxiv - Immunology 2024Quote: ... the pAC-Neo-OVA plasmid from Addgene (#22533) was utilized ...
-
bioRxiv - Genetics 2024Quote: Human pcDNA3-FLAG-RBBP5 plasmid used in the protein expression experiments was obtained from Addgene (Cat# 15550, MA, USA). Candidate variants were introduced using QuikChange II Site-directed mutagenesis kit according to manufacturer’s protocol (Agilent ...
-
bioRxiv - Bioengineering 2024Quote: ... Firefly luciferase was PCR amplified from pCDNA-Luciferase (Addgene, cat # 18964) using primers introducing a 5’ KpnI site and a 3’ NheI site ...
-
bioRxiv - Genomics 2024Quote: ... and transfected with 500 ng pMD-VSVg (Addgene #12259), 1,750 ng psPax2 (Addgene #12260) ...
-
bioRxiv - Genomics 2024Quote: ... the 1 μg of total DNA was split in a 1:15 ratio of pCAG-NLS-Bxb1 (Addgene #51271) and recombination vector ...
-
bioRxiv - Genomics 2024Quote: ... 1,750 ng psPax2 (Addgene #12260), and 1,750 ng landing pad G384A vector template using 6 μL Fugene 6 (Promega ...
-
bioRxiv - Genomics 2024Quote: ... and the top two scoring sgRNAs for each target gene based on depletion or enrichment phenotypes were cloned into dual sgRNA lentivirus expression vectors with direct capture tags (Addgene 187241)40 using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs #E2621L ...
-
bioRxiv - Genomics 2024Quote: ... and the top 3 sgRNAs were individually cloned into single sgRNA expression vectors with direct capture cs1 sequences (Addgene # 122238)10 using restriction digest (BstXI and BlpI ...
-
bioRxiv - Developmental Biology 2024Quote: ... and FSW-HRP-V5-LRRTM2 (Alice Ting, Addgene, #82537) plasmids ...
-
bioRxiv - Developmental Biology 2024Quote: ... gifted by Peter Scheiffele (Addgene, #59409), and inserted into pcDNA3.1(+ ...
-
bioRxiv - Immunology 2024Quote: ... 1.5μg each) and a dCas9-VPR plasmid (pLenti-dCas9-VPR-blast, Addgene, #96917; 1μg) as described above ...
-
bioRxiv - Genetics 2024Quote: ... whereas the pGL4.20-HIF1A prom plasmid was a gift from Alex Minella (Addgene plasmid # 40173 ; http://n2t.net/addgene:40173 ; RRID:Addgene_40173) and isolated by Zyppy Plasmid Miniprep Kit (Zymo Research Irvine ...
-
bioRxiv - Genetics 2024Quote: ... whereas the pGL4.20-HIF1A prom plasmid was a gift from Alex Minella (Addgene plasmid # 40173 ...
-
bioRxiv - Bioengineering 2024Quote: ... including existing parts (Addgene Kit # 1000000080) and custom-made parts (Table 2) ...
-
bioRxiv - Cell Biology 2024Quote: pAAV2/9n (Addgene plasmid #112865; http://n2t.net/addgene:112865; RRID: Addgene_112865) and pAdDeltaF6 (Addgene plasmid #112867 ...
-
bioRxiv - Cell Biology 2024Quote: pAAV2/9n (Addgene plasmid #112865 ...
-
bioRxiv - Microbiology 2024Quote: ... pXDC61 was a gift from Howard Shuman (Addgene plasmid #21841; http://n2t.net/addgene:21841; RRID:Addgene_21841). The in-frame deletion of dotG into the Legionella strain LP01 was generated using allelic exchange as previously described [39] ...
-
bioRxiv - Microbiology 2024Quote: ... pXDC61 was a gift from Howard Shuman (Addgene plasmid #21841 ...
-
bioRxiv - Microbiology 2024Quote: ... Plasmids expressing GST-tagged WT Dynamin 2 or Dynamin 2 ΔPRD were constructed using the Takara Biosciences In-Fusion system and a plasmid encoding rat WT Dynamin 2 (Addgene 34684) as a template ...
-
bioRxiv - Microbiology 2024Quote: ... and psPAX2 (plasmid no. 12260; Addgene) into HEK293T cells ...
-
bioRxiv - Microbiology 2024Quote: ... pMD2G (plasmid no. 12259; Addgene), and psPAX2 (plasmid no ...
-
bioRxiv - Molecular Biology 2024Quote: ... They were incorporated into lentiCRISPR-v2 (Addgene, Cat# 52961) following the GeCKO protocol with minor adjustments(Sanjana et al. ...
-
bioRxiv - Molecular Biology 2024Quote: ... and packaging plasmid (psPAX2) (Addgene, 12260) using Lipofectamine 3000 per the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2024Quote: ... and mTOR DNA constructs were obtained from Addgene (plasmid #12133, #14129, #1861)24 ...
-
bioRxiv - Microbiology 2024Quote: ... and the FLP recombinase expression plasmid (pFLP3, Addgene plasmid # 64946) were gifts from Herbert Schweizer [38].
-
bioRxiv - Microbiology 2024Quote: ... Tn7 transposase expression plasmid (pTNS, Addgene plasmid # 64967), and the FLP recombinase expression plasmid (pFLP3 ...
-
bioRxiv - Molecular Biology 2024Quote: ... pcDNA3.1-FLAG-gp130:sarA chimera codon-optimized for mammalian expression were previously described in and available from Addgene. Site-directed mutagenesis was carried out using Quikchange Lightning Site-Directed Mutagenesis Kit (Agilent ...
-
bioRxiv - Molecular Biology 2024Quote: ... 100 ng of CB1-GFP and 100 ng of Rab5-BFP (Addgene #49147) were mixed with 0.2 µL of lipofectamine 2000 following transfection method previously described then added to the wells ...
-
bioRxiv - Molecular Biology 2024Quote: ... 100 ng of the EPAC sensor (Addgene #61622) were mixed with 0.2 µL of lipofectamine 2000 following the transfection method previously described ...
-
bioRxiv - Molecular Biology 2024Quote: ... 200 ng of hMbPylRS-4xU6M15 (Addgene, #105830) and 200 ng of the respective amber construct were premixed in 20 µL of DMEM ...
-
bioRxiv - Neuroscience 2024Quote: AAV serotype 5 EF1a-DIO-YFP (1.3 × 1013 gp/mL) (Addgene #27056)
-
bioRxiv - Neuroscience 2024Quote: AAV serotype 1 EF1a-FLEx-taCasp3-TEVp (5.8 × 1012 gp/mL) (Addgene #45580)49