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Citations for Addgene :
2751 - 2800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... FUGW (Addgene plasmid # 14883) was digested with XbaI and EcoRI ...
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bioRxiv - Bioengineering 2024Quote: ... Lamp1-mScarlet-I (Addgene plasmid # 98827) and mScarletI-Rab7 (Addgene plasmid # 112955 ...
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bioRxiv - Bioengineering 2024Quote: ... mScarlet-EEA1 (Addgene plasmid # 169067) and GFP-Rab5B (Addgene plasmid # 61802 ...
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bioRxiv - Bioengineering 2024Quote: ... and mScarletI-Rab7 (Addgene plasmid # 112955) were gifts from Dorus Gadella ...
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bioRxiv - Bioengineering 2024Quote: ... and GFP-Rab5B (Addgene plasmid # 61802) were gifts from Gia Voeltz ...
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bioRxiv - Biochemistry 2024Quote: ... pGL3-ELAM-luc was a gift from Doug Golenbock (Addgene plasmid # 13029 ...
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bioRxiv - Genomics 2024Quote: ... pB006 (Addgene plasmid #182320), a gift from Dr ...
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bioRxiv - Neuroscience 2024Quote: ... AAV2/2-hsyn-DIO-hM4D(Gi)-mCherry was obtained from Addgene (plasmid #44362, 4.6x1012 GC/ml). For optogenetic activation ...
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bioRxiv - Molecular Biology 2024Quote: ... and pRSV-Rev (Addgene #12253) with modified pCW57.1 target vector in a ratio of 5 ...
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bioRxiv - Molecular Biology 2024Quote: ... N-terminally TurboID TTP (TID-TTP) and empty TurboID (TID-E) vectors were generated by cloning TID from V5-TurboID-NES_pCDNA3 (Addgene#107169) into a modified TurboID-containing pCW57.1 vector (pCW57.1-pCW 57.1-MYC-TurboID-MCS-PGK-mCherry-P2A-rtTA) ...
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bioRxiv - Physiology 2024Quote: ... The latter consisted of the pLV6 backbone and a mouse per2 promoter with adjacent luciferase sequence contained in the pGL3 basic E2 vector (Addgene plasmid 48747). To ligate the Per2:luciferase reporter with pLV6 backbone we designed a restriction cloning approach shown to be efficient in large plasmids using the QuickChange Lightning Site-Directed Mutagenesis (SDM ...
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bioRxiv - Cancer Biology 2024Quote: ... The GeCKO v2 library A was obtained from Addgene (1000000052) and was amplified and packaged in lentiviruses as described previously(19) ...
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bioRxiv - Cell Biology 2024Quote: ... 2.3 µg pPAX2 (Addgene; 12260) and 0.85 µg pMD2G (Addgene ...
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bioRxiv - Genetics 2024Quote: ... Based on these results we replaced the intronized Cas9 with one that had no introns but retained the NLS sequences at the termini (Cas9 without introns from pTX168, which was a gift from Daniel Voytas, Addgene #89257)119 ...
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bioRxiv - Bioengineering 2024Quote: ... and FRB-CFP-Ras (Addgene ID: 87451) are available on Addgene ...
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bioRxiv - Bioengineering 2024Quote: YFP-FKBP-DCRD (Addgene ID: 87453), YFP-FKBP-PCRD (Addgene ID ...
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bioRxiv - Bioengineering 2024Quote: ... YFP-FKBP-PCRD (Addgene ID: 87452), and FRB-CFP-Ras (Addgene ID ...
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bioRxiv - Bioengineering 2024Quote: CHMP2A_GFP_N_term was a gift from Daniel Gerlich (Addgene plasmid # 31805). Lamp1-mScarlet-I (Addgene plasmid # 98827 ...
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bioRxiv - Bioengineering 2024Quote: ... with primers BW-NH-710 and BW-NH-711 while pTol2Dest was constructed by amplification of pT2/HE (Addgene Plasmid ID: 26557) with BW-NH-792 and BW-NH-793 ...
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bioRxiv - Biophysics 2024Quote: ... GPCR sequences were obtained from PRESTO-tango kit (Addgene Kit #1000000068). For the construction of SNAP-tag fused CD86 ...
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bioRxiv - Biophysics 2024Quote: ... MDCK -wildtype or -transformed cells were transfected with E-cadherin-GFP plasmid (Addgene) using Lipofectamine 2000 transfection reagent (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... pCAG_MMP-9 – full length mouse (MMP-9) from pcDNA3.1_MMP-9-HA (Addgene #121172) cloned into pCAG vector ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Lentiviral particles for CEBPB knockdown were generated by transfecting shRNA plasmids (TRCN0000007440 and TRCN0000007442) or scramble shRNA control (a gift from David Sabatini; Addgene_1864 [50]), psPAX2 (a gift from Didier Trono ...
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bioRxiv - Immunology 2024Quote: gBlocks™ (Integrated DNA Technologies, Coralville, IA) encoding VHH were ligated into the pcDNA3.1 mammalian expression vector (Addgene, Watertown, MA), in frame with a carboxy terminal E-tag or eGFP-tag for transient or stable transfection ...
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bioRxiv - Genomics 2024Quote: ... lentiviral vector by GenScript to make pUltra BCR-ABL WT (Addgene #210432). Twist Bioscience generated a saturating mutagenesis (SM ...
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bioRxiv - Genomics 2024Quote: BCR-ABL cDNA was cloned downstream of EGFP in the pUltra (Addgene #24129) lentiviral vector by GenScript to make pUltra BCR-ABL WT (Addgene #210432) ...
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bioRxiv - Developmental Biology 2024Quote: ... and the Dendra2 coding sequence was inserted to serve as the template for DNA integration (Addgene stock TBD). One-cell stage embryos obtained by natural crosses of Casper/Casper adults were co-injected with an injection mix including 150 ng/ µL of TALEN mRNA and 12 ng/µL of donor plasmid (Fig ...
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bioRxiv - Developmental Biology 2024Quote: In-frame integration of Dendra2 into the C-terminus of apoBb.1 was achieved using TALENs as previously described and validated (56) (Addgene stock 128695 and 128696). TALENs were in vitro transcribed using the T3 Message Machine Kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Target sequences were cloned into pU6-BbsI-chiRNA (Gratz et al., 2013; Addgene Plasmid 45946). The resulting plasmids were injected with or without donor plasmid into vasa-Cas9 expressing embryos (Sebo et al. ...
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bioRxiv - Genetics 2024Quote: ... a 3XFLAG-NatMX cassette was PCR-amplified from pYC46 plasmid (Addgene) and inserted at the C-terminus of ZCF27 and ZCF4 (74 ...
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bioRxiv - Genetics 2024Quote: ... and 3’ UTR was PCR-amplified and cloned into the pGRB2.0 plasmid (Addgene) (73 ...
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bioRxiv - Genetics 2024Quote: ... p415-GalL-Cas9-CYC1t (Addgene plasmid #:107922; MSp6) and transformants were selected on CSM -Ura -Leu glucose plates ...
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bioRxiv - Genetics 2024Quote: ... a HIS3 SpCas9 gRNA plasmid acquired from Jean-Marc Daran (Addgene plasmid #:107922). For cloning in the repair templates ...
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bioRxiv - Genetics 2024Quote: ... is a functional plasmid that was used as a basis for the recombination experiments (plasmid available at Addgene). Two modifications of this plasmid—a truncation to create a 5758bp plasmid p-att-ef1a-MS2-RecT-dCas9-BSD-truncated and an extension to create a 13942bp plasmid p-att-ef1a-MS2-RecT-dCas9-BSD-extended —were used for recombination experiments but are not intended to be functional for genome editing ...
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bioRxiv - Immunology 2024Quote: 293T cells were seeded in 6-well plates and transfected with the prISG15-Renilla-hPEST-IRES-Puro construct together with psPAX2 and pMD2.G (Addgene plasmids #12259 and #12260 ...
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bioRxiv - Immunology 2024Quote: ... and the hPEST sequence was PCR amplified from plasmid pcDNA3.1(+)GUG-nLuc-3XFLAG-CL1/PEST (Addgene plasmid #127317 ...
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bioRxiv - Immunology 2024Quote: eGFP as control or SMARCA4 were cloned into the pInducer20 doxycycline inducible lentiviral vector (Addgene 44012). Lentivirus was produced using standard virus production methods by co-transfecting target and packaging plasmids (psPAX2 – Addgene12260 and pMD2.G-Addgene 12259 ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa Cas9 cells were generated with the lentiCas9-Blast plasmid 87 (Addgene plasmid #52962 ...
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bioRxiv - Cell Biology 2024Quote: ... reporter cell lines were transfected with pCBASce (Addgene plasmid #26477; http://n2t.net/addgene:26477; RRID: Addgene_26477, a gift from Maria Jasin) plasmid using Lipofectamine 3000 according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... reporter cell lines were transfected with pCBASce (Addgene plasmid #26477 ...
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bioRxiv - Cell Biology 2024Quote: The DSB repair reporter vectors pDRGFP (Addgene plasmid #26475; http://n2t.net/addgene:26475; RRID: Addgene_26475) and hprtSAGFP (Addgene plasmid #41594 ...
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bioRxiv - Cell Biology 2024Quote: ... and hprtSAGFP (Addgene plasmid #41594; http://n2t.net/addgene:41594; RRID: Addgene_41594), were a gift from Maria Jasin ...
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bioRxiv - Cell Biology 2024Quote: The DSB repair reporter vectors pDRGFP (Addgene plasmid #26475 ...
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bioRxiv - Cell Biology 2024Quote: ... Reporter cells were transfected in parallel with pCAGGS-mCherry (a gift from Phil Sharp; Addgene plasmid #41583 ...
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bioRxiv - Cell Biology 2024Quote: ... Reporter cells were transfected in parallel with pCAGGS-mCherry (a gift from Phil Sharp; Addgene plasmid #41583; http://n2t.net/addgene:41583; RRID: Addgene_41583) as an internal control to estimate transfection efficiency and calculate relative DSB repair activity ...
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bioRxiv - Cell Biology 2024Quote: ... and hprtSAGFP (Addgene plasmid #41594 ...
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bioRxiv - Cell Biology 2024Quote: The plasmid EF1a-mito-dsRED2 was a gift from Thomas Schwarz (Addgene plasmid # 174541 ...
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bioRxiv - Cell Biology 2024Quote: The plasmid EF1a-mito-dsRED2 was a gift from Thomas Schwarz (Addgene plasmid # 174541; http://n2t.net/addgene:174541; RRID:Addgene_174541). It contains a Flex-Switch-MitoDsRed construct under the transcriptional control of the EF-1α promoter ...
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bioRxiv - Cell Biology 2024Quote: ... were constructed by inserting annealed synthetic oligomers (5′-CACCgcagctcctgcctctcatcg-3′ and 5′-AAACcgatgagaggcaggagctgc-3′) into the Bbs I site of pX330-U6-Chimeric_BB-CBh-hSpCas9 vector (#42230; Addgene, Watertown ...
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bioRxiv - Cell Biology 2024Quote: ... The iRFP670 sequence was cloned into pNLS-iRFP670 (#45466; Addgene) (Shcherbakova and Verkhusha ...