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Citations for Addgene :
2401 - 2450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-hSyn1-GcaMP6s-P2A-nls-dTomato (which was a gift from Jonathan Ting [Addgene plasmid # 51084 ...
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bioRxiv - Neuroscience 2024Quote: pGP-AAV-syn-jGCaMP7c variant 1513-WPRE was a gift from Douglas Kim & GENIE Project (Addgene viral prep # 105321-AAV1; http://n2t.net/addgene:105321; RRID: Addgene_105321)54 ...
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bioRxiv - Molecular Biology 2024Quote: ... pKI1.1R plasmid (Addgene #85808) was linearized by incubating 1.5 µg of the plasmid with AarI restriction enzyme for 16 h ...
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bioRxiv - Synthetic Biology 2024Quote: ... RfxCas13d were amplified from pY108 (Addgene #84739), pXR001 (#84739) ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH10B M.XmnI (Addgene, 114269)34 and plasmid DNA was purified using the QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Synthetic Biology 2024Quote: ... This plasmid is available from Addgene (#138589). A version of this plasmid encoding the Sav S112F K121Q mutant was used as the starting point for library generation.
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-hSyn1-GcaMP6s-P2A-nls-dTomato (which was a gift from Jonathan Ting [Addgene plasmid # 51084; http://n2t.net/addgene:51084; RRID: Addgene_51084]), respectively8,34 ...
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bioRxiv - Neuroscience 2024Quote: ... gene fragments of GcaMP6s and P2A were obtained from pGP-CMV-GcaMP6s-WPRE (which was a gift from Douglas Kim & GENIE Project [Addgene plasmid # 40753 ...
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bioRxiv - Neuroscience 2024Quote: ... gene fragments of GcaMP6s and P2A were obtained from pGP-CMV-GcaMP6s-WPRE (which was a gift from Douglas Kim & GENIE Project [Addgene plasmid # 40753; http://n2t.net/addgene:40753; RRID: Addgene_40753]), and pAAV-hSyn1-GcaMP6s-P2A-nls-dTomato (which was a gift from Jonathan Ting [Addgene plasmid # 51084 ...
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bioRxiv - Synthetic Biology 2024Quote: ... pORTMAGE-Ec1 was a gift from the lab of George Church (Addgene plasmid #138474; http://n2t.net/addgene:138474; RRID:Addgene_138474). pUC19 was acquired from New England Biolabs ...
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bioRxiv - Synthetic Biology 2024Quote: ... pORTMAGE-Ec1 was a gift from the lab of George Church (Addgene plasmid #138474 ...
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bioRxiv - Synthetic Biology 2024Quote: ... carrying a previously constructed helper plasmid (24) (pDY118A, Figure S1, Addgene ID 182958). pDY118A encodes SsrA-tagged SpCas9 (10 ...
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bioRxiv - Synthetic Biology 2024Quote: ... sgRNAs containing 20-nucleotide (nt) spacer sequences to direct Cas9 cleavage were encoded on donor or guide plasmids constructed from pAM041 (Figure S2, Addgene ID 217969) as described below ...
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bioRxiv - Neuroscience 2024Quote: ... were cloned by ligating annealed oligonucleotide pairs (Supplemental table 5) into the BsmBI-digested pBPK1520 plasmid (BPK1520 was a gift from Keith Joung. Addgene#65777 ...
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bioRxiv - Neuroscience 2024Quote: ... Lentiviral particles were produced in the HEK293T cell line using psPAX2 (12260, Addgene) and pMD2.G (12259 ...
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bioRxiv - Neuroscience 2024Quote: ... cells were transduced with an inducible system Tet-pLKO-puro (21915, Addgene) encoding an shRNA targeting TARDBP sequence (TRCN0000016038 ...
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bioRxiv - Neuroscience 2024Quote: ... and pMD2.G (12259, Addgene) plasmids (both a gift from Dr ...
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bioRxiv - Neuroscience 2024Quote: ... peGFP-N1-FLAG (Addgene plasmid # 60360).
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bioRxiv - Systems Biology 2024Quote: ... and pMW#3 (Addgene #13350) destination vectors using LR Clonase (ThermoFisher #11791100) ...
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bioRxiv - Neuroscience 2024Quote: AAV serotype 5 OTp-hM3Dq-Myc (2.9 × 1012 gp/mL) (Corresponding plasmid: Addgene #184753)84
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bioRxiv - Neuroscience 2024Quote: ... The AAV serotype DJ hSyn-DIO-hM4Di-mCherry (6.9 × 1012 gp/mL) was generated by the Fukushima Medical University School of Medicine Viral Vector Core facility with the corresponding plasmid (Addgene #44362).
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bioRxiv - Neuroscience 2024Quote: AAV serotype 1 CaMK2a-EYFP (1.0 × 1013 gp/mL) (Addgene #105622)
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bioRxiv - Systems Biology 2024Quote: ... coli origin of replication and ampicillin resistance gene were PCR amplified from pCRISPRyl (Addgene #70007) (Schwartz et al. ...
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bioRxiv - Systems Biology 2024Quote: ... PpHIS4 gene was PCR amplified from pMJA089 (Addgene #128518) (Yang et al. ...
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bioRxiv - Systems Biology 2024Quote: ... PCR amplified constructs were inserted into mCherry-CRY2clust (Addgene #105624), using BsrGI/HpaI sites to obtain mCherry tagged JNK2α2 and SgrAI/HpaI sites to obtain mCherry tagged ASK1 constructs ...
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bioRxiv - Systems Biology 2024Quote: ... pPBbsr-JNKKTR-mCherry plasmid containing JNK activity reporter (JNK-KTR) was obtained from Addgene (#115493). mCherry in this plasmid was exchanged with iRFP713 (from Addgene #111510 ...
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bioRxiv - Systems Biology 2024Quote: ... ClopHensor plasmid was obtained from Addgene (#25938). JNK2α2 and ASK1 were amplified from pCDNA3 Flag Jnk2a2 (Addgene #13755 ...
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bioRxiv - Systems Biology 2024Quote: ... mCherry in this plasmid was exchanged with iRFP713 (from Addgene #111510) fluorophore using NotI and SalI enzymes ...
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bioRxiv - Systems Biology 2024Quote: ... JNK2α2 and ASK1 were amplified from pCDNA3 Flag Jnk2a2 (Addgene #13755) and pCMV6-Myc-MAP3K5 (ASK1 ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... 2 μg pMD2.G (Addgene #12259), and 36 μL Turbofect (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... into the BsmBI-digested pBPK1520 plasmid (BPK1520 was a gift from Keith Joung. Addgene#65777; http://n2t.net/addgene: 65777; RRID:Addgene_65777)138 ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli chromosome at the λ integration site by electroporating this plasmid into DH10B cells containing the helper plasmid pInt-ts (Addgene #66076) and selecting for kanamycin resistant colonies ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH10GFP (Addgene #109392), a strain constructed in the same way in the prior study of burden by Ceroni et al.21
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bioRxiv - Synthetic Biology 2024Quote: ... we inserted the constitutive GFP expression cassette cloned into pAH63 (Addgene #66073) into the E ...
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bioRxiv - Neuroscience 2024Quote: ... into the BsaI-digested pU6-peg-GG-acceptor (pU6-pegRNA-GG-acceptor was a gift from David Liu. Addgene #132777; http://n2t.net/addgene: 132777; RRID:Addgene_132777). Prime editing nicking guide plasmids (ngRNAs ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli BW25113 carrying pDY118A using a donor plasmid (pKAW086, Figure S3, Table S2, Addgene ID 217967) containing an editing cassette comprised of kanR flanked by regions of homology to the genome (Table S6) ...
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bioRxiv - Neuroscience 2024Quote: ... Plasmid based prime editing: 500 ng pCMV-PE2-GFP (a gift from David Liu, Addgene#132776)115 ...
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bioRxiv - Neuroscience 2024Quote: ... pegRNA plasmids for prime editing were cloned by ligating annealed oligonucleotide pairs (Supplemental table 5) into the BsaI-digested pU6-peg-GG-acceptor (pU6-pegRNA-GG-acceptor was a gift from David Liu. Addgene #132777 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The editing cassette (Table S7) was amplified from pKAW066 (Figure S4, Table S2, Addgene ID 217968) using the manufacturer’s protocol for NEB Q5 Hot Start DNA Polymerase (M0494L ...
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bioRxiv - Synthetic Biology 2024Quote: ... and eleven were procured from Addgene (Watertown, MA, USA). One plasmid solution was taken from a transfection kit where the mixture contains two plasmids with the same vector backbone ...
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bioRxiv - Neuroscience 2024Quote: ... or AAVretro-Cre (pAAV-Ef1a-mCherry-IRES-Cre, 55632-AAVrg, 1.1-3.5 x 1012 viral genomes/ml, Addgene) into the ipsilateral posterior medial nucleus of the thalamus (POM) ...
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bioRxiv - Neuroscience 2024Quote: ... AAV2/5.CaMKII.tdTomato (Neurophotonics, 661-aav5) was used to label excitatory neurons and AAV9.CaMKII.GCaMP6s.WPRE.SV40 (Addgene, 107790) was employed to image excitatory neuronal calcium activity ...
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bioRxiv - Neuroscience 2024Quote: ... 2.3 nL of AAV1-CAG-tdTomato (59462-AAV1; Addgene) were injected twice ...
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bioRxiv - Synthetic Biology 2024Quote: ... pUS116 (6.6 kb) (AddGene #84578) is a Mycobacterium-E ...
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bioRxiv - Synthetic Biology 2024Quote: ... all plasmids deposited to Addgene were independently subjected to whole-plasmid sequence confirmation by Addgene.
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bioRxiv - Synthetic Biology 2024Quote: ... These parts were constructed by obtaining a template plasmid from Addgene, amplifying the desired fragment by PCR with primers that introduce the required SapI cut sites and overhangs ...
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bioRxiv - Synthetic Biology 2024Quote: ... pIB184-Km (Addgene plasmid # 90195) and pIB185 (Addgene plasmid # 90196 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmids pIB166 (Addgene plasmid # 90189), pIB184-Km (Addgene plasmid # 90195 ...
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bioRxiv - Systems Biology 2024Quote: ... pcDNA3 VP4:VP2 (Addgene, 216787) was prepared by PCR cloning of amino acids 1–332 from pcDNA3 CVB3 into the BamHI–EcoRI sites of pcDNA3 ...
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bioRxiv - Systems Biology 2024Quote: ... 4.5 μg of psPAX2 (Addgene #12260), 1.5 μg of pMD2.G (Addgene #12259 ...