Labshake search
Citations for Addgene :
2501 - 2550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2024Quote: ... was a gift from Howard Hang (Addgene plasmid #122650 ...
-
bioRxiv - Biophysics 2024Quote: The expression plasmid for HIS-tagged AavLEA1 was obtained from the Addgene plasmid repository [pET15b-AavLEA1 was a gift from Claude Férec (Addgene plasmid # 53093)]52 The expression plasmid for HIS-tagged RvLEAMshort was made by inserting a truncated cDNA from pEThT-RvLEAM49 [pEThT-RvLEAM was a gift from Takekazu Kunieda (Addgene plasmid # 90033)] ...
-
bioRxiv - Biophysics 2024Quote: The coding sequence of human fascin1 (GeneBank, NM_003088.4) was obtained from Addgene (#31207) and subsequently inserted into a pGEX-6p-1 vector (Cytiva ...
-
bioRxiv - Biophysics 2024Quote: ... using pCSII-EF/mt-(n1)StayGold (Addgene, no. 185823) as the template ...
-
bioRxiv - Biophysics 2024Quote: ... emiRFP670 were PCR amplified from Addgene Plasmid #136571 (Matlashov et al. ...
-
bioRxiv - Biophysics 2024Quote: ... into a pET His6 TEV LIC cloning vector (a gift from Scott Gradia, Addgene plasmid # 29653; http://n2t.net/addgene:29653; RRID:Addgene_29653). We designed oligomerisation-deficient constructs (D123P ...
-
bioRxiv - Biophysics 2024Quote: ... University of Cambridge) into a pET His6 TEV LIC cloning vector (a gift from Scott Gradia, Addgene plasmid # 29653 ...
-
bioRxiv - Biophysics 2024Quote: ... the plasmid pMSP1E3D1 (Addgene) encoding for MSP1E3D1 was expressed in E.coli and purified using the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... Ovalbumin-expressing B16F10 (B16F10-OVA) was established with pCI-neo-mOVA plasmid (Addgene plasmid #25099) and selected with 1 mg/ml of G418 for 2 weeks as previously described.42,43 All cell lines were maintained at < 70% in culture and tested for mycoplasma contamination every 2 weeks according to MCTP standard protocols.
-
bioRxiv - Cell Biology 2024Quote: ... pBOB-EF1-FastFUCCI-Puro was a gift from Kevin Brindle & Duncan Jodrell (Addgene plasmid # 86849 ...
-
bioRxiv - Cell Biology 2024Quote: ... plasmids were purchased from Addgene (Addgene#1817 ...
-
bioRxiv - Cell Biology 2024Quote: ... pBOB-EF1-FastFUCCI-Puro was a gift from Kevin Brindle & Duncan Jodrell (Addgene plasmid # 86849; http://n2t.net/addgene:86849; RRID: Addgene_86849) (Koh et al ...
-
bioRxiv - Cell Biology 2024Quote: The AAVS1 destination vector was assembled by PCR amplification and Gibson assembly from the pDTA-TK from Robert Benezra’s group (RRID:Addgene_22677) (Nam and Benezra 2009 ...
-
bioRxiv - Cell Biology 2024Quote: ... Cell lines for LysoIP were generated using constructs obtained from Addgene, details below ...
-
bioRxiv - Cell Biology 2024Quote: ... The lentiviral vector lentiCRISPRv2 carrying both Cas9 enzyme and a gRNA transfected into HEK293T cells together with the packaging plasmids psPAX2 and pCMV-VSV-G (Addgene) at the ratio of 5:3:2 ...
-
bioRxiv - Cell Biology 2024Quote: ... which were gifts from Julie Ahringer (Addgene plasmids #21506 and #22513) (Zeiser et al. ...
-
bioRxiv - Cell Biology 2024Quote: ... Clover and mScarletI sequences were obtained from Addgene plasmids 40259 and 85044 ...
-
bioRxiv - Cell Biology 2024Quote: ... for endogenous tagging and ATG14 knockout were designed using Benchling online platform [https://benchling.com] and inserted via BsaI sites into the pU6-(BsaI)_CBh-UN-Cas9 plasmid (Addgene 135011) [34] for endogenous tagging and BbsI sites forpSpCas9(BB)-2A-Puro (PX459 ...
-
bioRxiv - Cell Biology 2024Quote: Plasmids for mammalian expression of emIRFP670 and mIRFP713 under the control of CMV promoter were obtained from Addgene vectors #136556 and #136559 ...
-
bioRxiv - Cell Biology 2024Quote: ... were generated by RT-PCR from mouse cDNA cloned (details available on request) into a modified doxycycline-inducible lentiviral vector pCW57.1 (a gift from David Root, Addgene plasmid # 41393). 293 [HEK-293] (ATCC® CRL-1573TM ...
-
bioRxiv - Cell Biology 2024Quote: ... was designed with the CRISPR design tool at Benchling (www.benchling.com) and cloned into vector pSpCas9(BB)-GFP (a gift from Feng Zhang, Addgene plasmid #48138) as described [85,91] ...
-
bioRxiv - Cell Biology 2024Quote: ... pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259; http://n2t.net/addgene:12259; RRID:Addgene_12259). psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260 ...
-
bioRxiv - Cell Biology 2024Quote: ... pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259 ...
-
bioRxiv - Biophysics 2024Quote: The expression plasmid for HIS-tagged AavLEA1 was obtained from the Addgene plasmid repository [pET15b-AavLEA1 was a gift from Claude Férec (Addgene plasmid # 53093)]52 The expression plasmid for HIS-tagged RvLEAMshort was made by inserting a truncated cDNA from pEThT-RvLEAM49 [pEThT-RvLEAM was a gift from Takekazu Kunieda (Addgene plasmid # 90033)] ...
-
bioRxiv - Biophysics 2024Quote: ... was a gift from Howard Hang (Addgene plasmid #122650; http://n2t.net/addgene:122650; RRID:Addgene_122650). We introduced the following modifications ...
-
bioRxiv - Cell Biology 2024Quote: ... templates were constructed by cloning the 0.9-1.2 kb upstream and downstream homology arms into pPD95.77 vector (Addgene #37465) using In-Fusion® HD Cloning Kit (Takara Bio ...
-
bioRxiv - Cell Biology 2024Quote: ... 20 bp CRISPR-Cas9 targets (Table S2) were inserted into the pDD162 vector (Addgene #47549) by linearizing this vector with 15 bp overlapped primers ...
-
bioRxiv - Cell Biology 2024Quote: ... HeLa cells transiently co-expressing individual GFP-tagged RUSH reporter and GalT-mCherry were cultured in complete DMEM supplemented with16 nM His-tagged streptavidin (in-house purified using Addgene #20860, a gift plasmid from A. Ting)(Howarth et al. ...
-
bioRxiv - Cell Biology 2024Quote: ... psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260; http://n2t.net/addgene:12260; RRID:Addgene_12260). Single cell clones were obtained by FACS ...
-
bioRxiv - Cell Biology 2024Quote: ... The cells were co-transfected with a single gRNA and pCAS9-mCherry empty (Addgene, 80975 (Schmid-Burgk et al., 2016)) using TransIT-2020 (Mirus ...
-
bioRxiv - Cell Biology 2024Quote: ... psPAX2 (Addgene,12260) and pLL3.7-GFP-FLAG- APEX2-ePTS1 or pLL3.7-GFP-FLAG-APEX2 using TransIT-2020 (MirusBio ...
-
bioRxiv - Cell Biology 2024Quote: ... psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260 ...
-
bioRxiv - Cell Biology 2024Quote: ... The LAMP1-RFP plasmid (Addgene plasmid 1817), kindly provided by Ori Avinoam ...
-
bioRxiv - Cell Biology 2024Quote: Knockout of ATG14 in single-tagged GABARAPs reporter cells was achieved by transfecting the desired cell line with pSpCas9(BB)-2A-Puro (PX459) V2.0 plasmid (Addgene 62988) expressing ATG14-targeting sgRNA ...
-
bioRxiv - Cell Biology 2024Quote: ... [34] for endogenous tagging and BbsI sites forpSpCas9(BB)-2A-Puro (PX459) V2.0 plasmid (Addgene 62988), for ATG14 knockouts ...
-
bioRxiv - Cell Biology 2024Quote: ... and pLKO.1 ShCtrl and psPAX2 (#12260) and pMD2G (#12259) (Addgene, Cambridge, MA, USA) were used ...
-
bioRxiv - Cell Biology 2024Quote: ... was cloned into the pEF-GFP plasmid (Addgene #11154) by replacing the GFP ...
-
bioRxiv - Cell Biology 2024Quote: ... and a pair of TALENs (Addgene # 59025, # 59026) through co-electroporation into hPSCs ...
-
bioRxiv - Cell Biology 2024Quote: ... hP53DD fragment was cloned into PEmax-P2A-hMLH1dn (Addgene #174828) at the C-terminus (hP53DD-PEmax-P2A-hMLH1dn ...
-
bioRxiv - Cell Biology 2024Quote: ... and pU6-tmpknot-GG-acceptor (Addgene #174039). These fragments were then assembled with the linearized lentivirus vector to generate the lenti-pegRNA-GG-acceptor and lenti-tmpknot-GG-aceptor backbone plasmids under hU6 promoter ...
-
bioRxiv - Cell Biology 2024Quote: ... the BSD fragment in pCMV-PEmax-P2A-BSD (Addgene # 174821) was first replaced with the hP53DD fragment to generate pCMV-PEmax-hP53DD ...
-
bioRxiv - Cell Biology 2024Quote: ... the lenti sgRNA(MS2)_puro plasmid (Addgene #73797) was linearized with BamHI and NdeI ...
-
bioRxiv - Cell Biology 2024Quote: ... PEmax or PE-Plus were constructed by replacing Cas9 in the Hygro-Cas9 donor plasmid (Addgene #86883) with the respective PE2 ...
-
bioRxiv - Cell Biology 2024Quote: ... through BbsI site or into lenti-sgRNA blast (Addgene #104993) through BsmbI site ...
-
bioRxiv - Cell Biology 2024Quote: ... pCMV-PEmax-P2A-BSD (Addgene # 174821) or the cloned PE-Plus.
-
bioRxiv - Cell Biology 2024Quote: ... Fragment containing the RFP dropout cassette and the fragment containing the RFP cassette along with the mpknot motif were separately amplified from pU6-pegRNA-GG-aceptor (Addgene #132777) and pU6-tmpknot-GG-acceptor (Addgene #174039) ...
-
bioRxiv - Cell Biology 2024Quote: ... Nicking sgRNA for PE3 editing was cloned into LsgRNA backbone (Addgene #47108) through BbsI site or into lenti-sgRNA blast (Addgene #104993 ...
-
bioRxiv - Cell Biology 2024Quote: ... which were amplified from pCMV-PE2 (Addgene #132775), pCMV-PEmax-P2A-BSD (Addgene # 174821 ...
-
bioRxiv - Cell Biology 2024Quote: ... we used the lentiCRISPRv2 vector (that includes a puromycin-selection cassette; a gift from Dr. Feng Zhang, Addgene, Ref. 52961), and its blasticidin or hygromycin derivatives (a gift from Dr ...
-
bioRxiv - Cell Biology 2024Quote: ... Lentiviral vectors were packaged using packaging plasmid (pCMV delta R8.2 dvpr, Addgene #8455) and the envelope plasmid (pCMV-VSV-G ...