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Citations for Addgene :
2801 - 2850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and pGEX-4T2-14-3-3 tau (θ) (Addgene #13281) were gifts from Michael Yaffe (Yaffe et al ...
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bioRxiv - Immunology 2024Quote: ... pcDNA-HA-14-3-3σ (Addgene #11946) and pGEX-4T2-14-3-3 tau (θ ...
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bioRxiv - Immunology 2024Quote: ... pGP-CMV-GCaMP6s (GCaMP6) construct was a gift from Douglas Kim (Addgene plasmid # 40753). TCRζ-Turquoise was generated by cloning turquoise sequence in plasmid expressing TCRζ with AgeI-NotI sites ...
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bioRxiv - Immunology 2024Quote: ... pCS2-HA-14-3-3ζ (Addgene #116888) and pCS2-HA-14-3-3η (Addgene #116887 ...
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bioRxiv - Immunology 2024Quote: The plasmid pRK5-myc-CDC42 obtained from Addgene encodes for the ubiquitous isoform 1 of CDC42 and contains a myc tag in N-terminal ...
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bioRxiv - Immunology 2024Quote: ... The sequence was subcloned into an MSCV GFP vector backbone (Addgene 91975). HEK293T cells were transfected with hCD19t MSCV GFP and ampho retroviral packaging vector ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 1.4 µg of DRD1-TANGO or DRD5-TANGO plasmid DNA (Plasmids #66268 or #66272, Addgene, Watertown, MA) and 120 µM CaCl2 (diluted with H2O from 2 mM stock ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... DRD1-TANGO or DRD5-TANGO plasmid DNA were created by Bryan Roth and obtained from Addgene (Plasmids #66268 or #66272 ...
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bioRxiv - Physiology 2024Quote: ... Cells were transfected with 2.5 mg of α-actinin-RFP (Addgene Cat. No: 58050). Pictures were obtained using a spinning-disk Zeiss 3i microscope at 25 frames per second for 10 seconds ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The β-arrestin2 green fluorescent protein (GFP) plasmid DNA was originally created by Robert Lefkowitz and obtained from Addgene (plasmid #35411 ...
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bioRxiv - Physiology 2024Quote: ... AAV-CRE (AAV8.TBG.PI.Cre.rBG) and AAV-GFP (AAV8.TBP.PI.eGFP.WPRE.bGH) were acquired from Addgene (#107787-AAV8 and #105535-AAV8). AAV-hNRF1 was produced by the University of Pennsylvania Vector Biocore and is described in our previous work48.
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bioRxiv - Genomics 2024Quote: ... and psPAX2 (Addgene, Cat #12260) using Lipofectamine 3000 (Lifetech ...
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bioRxiv - Developmental Biology 2024Quote: ... The U6x:sgRNAs were assembled into a contiguous sequence in the pGGDestTol2LC-4sgRNA vector (Addgene plasmid 64242) by Golden Gate ligation ...
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bioRxiv - Immunology 2024Quote: ... each RBD variant was tagged with a unique 26-nucleotide (N26) barcode via PCR and assembled into Yeast surface display vector (Addgene, 166782). The XBB.1.5 and JN.1 DMS libraries were further transfected into electrocompetent DH10B cells for plasmid amplification and proceed to PacBio sequencing library preparation to decipher the association between RBD variant and corresponding N26 barcode ...
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bioRxiv - Immunology 2024Quote: ... Lentivirus was produced using standard virus production methods by co-transfecting target and packaging plasmids (psPAX2 – Addgene12260 and pMD2.G-Addgene 12259) into HEK293T cells ...
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bioRxiv - Genetics 2024Quote: ... All the sgRNAs were expressed using the U6- promoter and subcloned into the PX458 vector (Addgene ID: 112220) to construct the PX458-sgRNAs targeting vector ...
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bioRxiv - Immunology 2024Quote: ... and pMD2.g (Addgene cat#12259) using Lipofectamine 3000 (ThermoFisher cat# L3000001 ...
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bioRxiv - Immunology 2024Quote: All constructs were cotransfected into HEK293T cells with lentiviral packaging vectors psPAX (Addgene cat#12260) and pMD2.g (Addgene cat#12259 ...
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bioRxiv - Genetics 2024Quote: ... were mixed with 500ng pCAG-loxp-stop-loxp-ZsGreen (Addgene, 51269) plasmid ...
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bioRxiv - Genetics 2024Quote: Guide RNA sequences were cloned into pCFD4-U6:1_U6:3tandemgRNAs was a gift from Simon Bullock (Addgene plasmid # 49411; http://n2t.net/addgene:49411 ; RRID:Addgene_49411) (Port et al ...
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bioRxiv - Genetics 2024Quote: ... Self-purified Protein AG-MNase (pDNA was from Addgene; 123461) was used ...
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bioRxiv - Genetics 2024Quote: ... The promoter region from the pCAGGS-mCherry vector (Addgene #41583) was combined with the human ACE2 CDS (Addgene #1786 ...
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bioRxiv - Genetics 2024Quote: ... breast cell lines were transduced with Lenti-Cas9-2A-Blast (Addgene #73310) or Lenti-dCas9-KRAB-Blast (Addgene #89567) ...
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bioRxiv - Genetics 2024Quote: ... lentiviral pooled library was obtained from Addgene (Addgene, # 1000000048) and was prepared as previously described 41 ...
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bioRxiv - Immunology 2024Quote: Plasmids pMD2.G and psPAX2 were gifts from Didier Trono (Addgene plasmid # 12259; # 12260). Full length FcγRIIIa variant sequences were cloned under the CMV promoter in pCDH-CMV-MCS- EF1α-copGFP (System Biosciences) ...
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bioRxiv - Genomics 2024Quote: ... and a transfer vector (Fuw-dCas9-DNMT3A-P2A-tagBFP -Addgene #84569-; Fuw-dCas9-TET1-P2A-tagBFP -Addgene #108245- and pLV GG hUBC-dsRED -Addgene #85034- ...
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bioRxiv - Genetics 2024Quote: The human GeCKO-v2 (Genome-Scale CRISPR Knock-Out) lentiviral pooled library was obtained from Addgene (Addgene, # 1000000048) and was prepared as previously described 41 ...
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bioRxiv - Genetics 2024Quote: ... 5’- and 3’-homologous arms were added to both ends. Two gRNA sequences were cloned into plasmid pCFD4 ((Port et al. 2014) (Addgene). The donor and gRNA plasmids were injected into a Cas9 line (y[1] M{vas-Cas9.S}ZH-2A w[1118] ...
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bioRxiv - Genetics 2024Quote: The CAG-ACE2 genetic construct was prepared on the basis of the pT2/BH backbone (BglII, EcoRI sites) (Addgene #26556). The promoter region from the pCAGGS-mCherry vector (Addgene #41583 ...
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bioRxiv - Genetics 2024Quote: ... early passage primary MEFs from all genotypes were infected in parallel with the SV40 large T antigen (Addgene:13970) (Zhao et al. ...
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bioRxiv - Genetics 2024Quote: ... RRID:Addgene174038).23 BPK1520 was a gift from Keith Joung (Addgene plasmid #65777 ...
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bioRxiv - Genetics 2024Quote: pCMV-PEmax-P2A-GFP was a gift from David Liu (Addgene plasmid #180020 ...
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bioRxiv - Genetics 2024Quote: ... RRID:Addgene174824).22 The pU6-tevopreq1-GG-acceptor was a gift from David Liu (Addgene plasmid #174038 ...
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bioRxiv - Genetics 2024Quote: ... and blue fluorescent protein (tagBFP) with a KRAB domain at the C-terminus (Addgene 167981; Figure 1A)14 ...
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bioRxiv - Genetics 2024Quote: We use two constructs for epigenome editing: 1) dCAS9-DNMT3A/3L co-expressed with enhanced green fluorescent protein (eGFP) for selection 12 (Addgene, Catalogue number 128424), and 2 ...
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bioRxiv - Genetics 2024Quote: ... the top two candidate sgRNA sequences for each gene were cloned into lentiCRISPRv2 (Addgene # 52961) (Sanjana ...
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bioRxiv - Genetics 2024Quote: ... RRID:Addgene180020).22 pEF1a-hMLH1dn was a gift from David Liu (Addgene plasmid #174824 ...
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bioRxiv - Genetics 2024Quote: ... Guide RNAs were ordered as dsDNA from Integrated DNA Technologies (Supplemental Table S1) and cloned into the expression vector (Addgene 44248) by double digest with BstXI and NotI (Thermo Fischer Science) ...
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bioRxiv - Genetics 2024Quote: ... or Lenti-dCas9-KRAB-Blast (Addgene #89567). MCF7 cells stably expressing Cas9 or KRAB-dCas9 were established by transduction of the virus at MOI<0.7 29 ...
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bioRxiv - Genetics 2024Quote: ... Pooled oligonucleotides were cloned into the BsmBI sites of pLeniGuide-Puro (Addgene #52963). We included as positive controls core cell essential genes and genes known to confer resistance to T cell-mediated killing ...
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bioRxiv - Genetics 2024Quote: ... The sequence-verified expression constructs for wild-type and mutated AARS1 were then cloned into either the pAG425 expression vector (pAG425GAL-ccdB; Addgene #14153 ...
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bioRxiv - Developmental Biology 2024Quote: ... David Sabatini and Kris Wood (Addgene plasmid #64613 ...
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bioRxiv - Genetics 2024Quote: ... and NGN2 (Addgene 99378) lentiviruses in StemFlex media containing 10 μM Thiazovivin (Millipore ...
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bioRxiv - Genetics 2024Quote: ... Barcoded fragments were inserted in the SbfI/AgeI site of the pLS-SceI vector (AddGene #13772) and then transformed into 10-beta competent cells (NEB ...
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bioRxiv - Genetics 2024Quote: ... To determine MOI and approximation of appropriate viral volume we infected day 14 iGLUTs (0, 1, 2, 4, 8, 10, 16, 32, 64 µL) with control lentivirus (pLS-SV40-mP-EGFP; AddGene #137724) and harvested for 48hrs later ...
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bioRxiv - Genetics 2024Quote: Clonal hiPSCs from two neurotypical donors of European ancestry with average schizophrenia PRS and no history of psychiatric diagnoses (#3182 (XX) and #2607 (XY)) were generated by lentiviral transduction with pLV-TetO-hNGN2-eGFP-Neo and lentiviral FUW-M2rtTA (Addgene #20342), followed by antibiotic selection and clonal expansion ...
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bioRxiv - Genetics 2024Quote: ... acquired from Addgene (plasmid # ...
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bioRxiv - Genetics 2024Quote: We used p-dCas9-SSAP-MS2-BB (Addgene Cat#183826) [62] as a convenient source of bacterial backbone and dCas9 for Gibson assembly ...
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bioRxiv - Genetics 2024Quote: ... You can use our plasmid (deposited at Addgene as dCas9-ABE8e-Blasticidin-MC-Plasmid #XXXXXXX ...
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bioRxiv - Developmental Biology 2024Quote: ... we first made a targeting construct using CRE-treated and BstBI/MluI digested LSL-Cas9-ROSA26TV plasmid donor (gift from Feng Zhang, #61408, Addgene) to subclone the DHB-mClover3_2A_H2B-mRuby3 fragment ...