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Citations for Addgene :
2551 - 2600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... PEX3 KO or PEX19 KO cells were co- transfected with pMD2.G (Addgene, 12259), psPAX2 (Addgene,12260 ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-APEX2 fragment was amplified form pcDNA3 APEX2-NES (Addgene, 49386 (Lam et al., 2015)) ...
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bioRxiv - Biochemistry 2024Quote: ... and pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-PSMB5-PSMB6-PSMB7-TEV-2xSTII (Addgene plasmid #XXXX) were assembled via restriction cloning utilizing the MultiBac multiplication module.
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bioRxiv - Biochemistry 2024Quote: ... MSPs in a pET vector (Addgene #20064 and #29520) was transformed into BL21-Gold (DE3 ...
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: pDisplay-LAP2-CFP-TM (Addgene plasmid #34842) and pYFJ16-LplA(W37V ...
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bioRxiv - Cancer Biology 2024Quote: ... was a gift from Boris Fehse (Addgene plasmid #154006) which was further modified to co-express hBCMA-tNGFR ...
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bioRxiv - Cell Biology 2024Quote: ... plasmids were purchased from Addgene (Addgene#1817, Addgene#98882, Addgene#102930 ...
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bioRxiv - Cell Biology 2024Quote: ... plasmids were purchased from Addgene (Addgene#1817 ...
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bioRxiv - Cell Biology 2024Quote: ... from the mCherry-Climp63 vector (Addgene#136293) (Shibata et al. ...
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bioRxiv - Biochemistry 2024Quote: ... MSP1E3D1(-) (a gift from Stephen Sligar, Addgene plasmid #20066 ...
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bioRxiv - Biochemistry 2024Quote: ... The cDNA of APLF full length and APLFΔFHA and APLFΔAD were amplified with respective primer pairs (Table S1) and subcloned into Flag-HA-pCDNA3.1 (Addgene #52535) (Horn et al. ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... or AAV5-hSyn-DIO-GFP virus (Addgene Cat. No. 50457-AAV5, 7x1012vg/mL). The viral injection procedures were executed as described in [32] ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... we bilaterally injected retrograde AAV expressing Cre-recombinase (AAVrg-hSyn-Cre-WPRE-hGH, Addgene) into the right PPC or bilateral STR (0.5 μl per each site ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... and injected ~0.5 μl of AAV-hSyn-ChrimsonR-tdTomato (Addgene) into the right M2 at two anterior-posterior (AP ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... we injected 0.5 μl of retrograde AAV expressing tdTomato (AAVrg-CAG-tdTomato, Addgene) and 0.5 μl of retrograde AAV expressing GFP (AAVrg-CAG-GFP ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... we injected ~0.5 μl of retrograde AAV expressing Cre-recombinase (AAVrg-hSyn-Cre, Addgene) into the right AC (bregma −2.5mm ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Mice were unilaterally injected using a Nanoject II programmable nanoliter injector with 600 nL/hemisphere with AAV5-hSyn-dLight1.2 (Addgene Cat. #111068-AAV5) [33] into the NAc core using stereotaxic bregma-based coordinates ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... and 0.5 μl of retrograde AAV expressing GFP (AAVrg-CAG-GFP, Addgene) into the target areas respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... The following plasmid was used: pCMV-Sport6-CD36-pHluorin (Plasmid#130901, Addgene). siRNA oligos were transfected into cells using Lipofectamine RNAiMax Reagent according to the manufacturer’s protocol (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: The Tet-pLKO-puro lentivirus vector (plasmid#21915) and the control shRNA lentivirus vector (pLKO-Tet-On-shRNA-Control, plasmid#398398) were purchased from Addgene (Watertown, USA). The construction of the two shRNA lentivirus vectors targeting the human β-catenin was performed following the Tet-pLKO Manual given by Addgene (plasmid#21915) ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... we injected ~0.5 μl of retrograde AAV expressing GCaMP6s (AAVrg-hSyn1-GCaMP6s-P2A-nls-dTomato, Addgene) into the right STR (bregma −1.5mm ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... heterozygous Adora2a-Cre mouse neonates were injected bilaterally with the Cre-recombinase-dependent viral vectors AAV5-hSyn- DIO-hM4D-mCherry virus (Addgene Cat ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... we injected ~0.5 μl of retrograde AAV expressing GCaMP6s (AAVrg-hSyn1-GCaMP6s-P2A-nls-dTomato, Addgene) into the right PPC (bregma −1.95mm ...
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bioRxiv - Bioengineering 2024Quote: ... with the packaging plasmid psPAX2 (Addgene) and the envelope-expressing plasmid PMD2.G (Addgene ...
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bioRxiv - Bioengineering 2024Quote: ... and the envelope-expressing plasmid PMD2.G (Addgene) using the transfection reagent CalFectin (SignaGen ...
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bioRxiv - Plant Biology 2024Quote: ... S1) targeting the OsNAC5 gene was designed and cloned into the pRGEB31 CRISPR/Cas9 vector (Addgene, Watertown, MA, USA) (Xie and Yang ...
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bioRxiv - Physiology 2024Quote: ... AAV8-TBG-Null (105536-AAV8, Addgene) was injected in floxed littermates of the control group ...
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bioRxiv - Neuroscience 2024Quote: ... AAV-mDlx-NLS-mRuby2 (Addgene plasmid #99130) and a plasmid for tagBFP in our lab stock (TRE-NLS-tagBFP ...
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bioRxiv - Physiology 2024Quote: ... and pcDNA3-Flag-FKHR-ΔDB-AAA (Addgene 9022, 9023, 10694) were kind gifts from Dr ...
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bioRxiv - Physiology 2024Quote: ... The EGFP-LC3B plasmid (Addgene 11546) was a kind gift from Dr ...
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bioRxiv - Physiology 2024Quote: ... L4440 was a gift from Andrew Fire (Addgene plasmid#1654 ...
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bioRxiv - Physiology 2024Quote: HEK 293T were transfected with a human TLR3 plasmid or an empty vector (Addgene). Briefly ...
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bioRxiv - Plant Biology 2024Quote: The long CaMV35S long promoter (Addgene #50267) and CaMV 35S terminator (Addgene #50337 ...
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bioRxiv - Plant Biology 2024Quote: ... and CaMV 35S terminator (Addgene #50337) were used in all the Cas protein expression cassettes ...
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bioRxiv - Plant Biology 2024Quote: ... A pCBC-DT1DT2 plasmid obtained from Addgene was used as the template for the assembly of two gRNA expression cassettes using a four-primer mixture in a high-fidelity PCR with Phusion High-Fidelity DNA Polymerase (Table S1) ...
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MYB68 orchestrates cork differentiation by regulating stem cell proliferation and suberin depositionbioRxiv - Plant Biology 2024Quote: ... and 3) the P336-FBP_11 vector (Available from AddGene (#139702)) ...
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bioRxiv - Plant Biology 2024Quote: ... cells harboring the plasmid p2CT-His-MBP-Lbu_C2c2_WT (Addgene No. 83482) were cultivated with 800 ml autoinduction medium (Studier ...
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bioRxiv - Plant Biology 2024Quote: 35S:GUS-pMAS:GFP was modified by replacing the 35S promoter with the MAS promoter from pGFPGUSPlus (Plasmid #64401, Addgene) (He et al. ...
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bioRxiv - Plant Biology 2024Quote: ... the low copy number pFA31 backbone (Addgene plasmid #162708; Flamholz et al., 2020) was modified into two terminal acceptors compatible with Loop Assembly (pFA-Odd and pFA-Even) ...
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bioRxiv - Plant Biology 2024Quote: ... Acceptors were pOdd1-4 (pCk1-4, Addgene plasmids # 136695-136698) for Level 1 and 3 and pEven1-4 (pCsA-E ...
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bioRxiv - Plant Biology 2024Quote: ... for Level 1 and 3 and pEven1-4 (pCsA-E, Addgene plasmids # 136067-136070) for Level 2 ...
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bioRxiv - Biophysics 2024Quote: ... FLAG-Mma PyIRS (Mma PylT/RS) and pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A (G1PylT/RS) tRNA/aminoacyl-tRNA synthetase pairs from Addgene (Watertown ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmid construction was performed by modular cloning using the MoClo Tool Kit and the MoClo Plant Parts Kit (Addgene, 28–30). A detailed MoClo protocol and the list of vectors used can be found in 18.
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bioRxiv - Plant Biology 2024Quote: ... and pICH51288 and pICH41414 (2×35S and 35S terminator; Addgene #50269 and #50337; Engler et al., 2014) in a BsaI Golden Gate reaction to generate binary vectors containing the fragment-swapped Rcr3/Pip1 hybrids driven by the double 35S CaMV promoter and targeted to the apoplast using a NtPR1a signal peptide ...
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bioRxiv - Biophysics 2024Quote: Human RING1b (Uniprot ID Q99496) and human BMI1 (Uniprot ID P35226) were cloned into a pFBOH-mhl vector (Addgene plasmid # 62304) cleaved with BseRI using Gibson Assembly® Master Mix (NEB #E2611L ...
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bioRxiv - Neuroscience 2024Quote: ... we cloned CreERT2 (Cre recombinase fused to a mutant ligand-binding domain of the estrogen receptor) and IRES into the BamHI site of pAAV-EF1a-tdTomato-WPRE-pGHpA (Addgene #67527). To prevent leakage of Cre activity34 ...
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bioRxiv - Neuroscience 2024Quote: ... we cloned an IRES-GFP sequence into the SpeI site of pJ241-FLEX (Addgene #18925). To drive expression of the FLEX cassette under the EF1a promoter ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... USA) and cloned into the T777T plasmid (Addgene plasmid # 113082; http://n2t.net/addgene:113082; RRID:Addgene_113082) which contains two IPTG-inducible T7 promoter (5’-TAATACGACTCACTATAG-3’ ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: For dsRNA vectors assembly, constructs sequences were synthesized in Integrated DNA Technologies (IDT, Coralville, IA, USA) and cloned into the T777T plasmid (Addgene plasmid # 113082 ...
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bioRxiv - Plant Biology 2024Quote: ... The CGR3 cassette was combined with a CaMV35S:BAR selection marker and cloned into the pCsB acceptor backbone (Addgene #136068) prior to electroporation into A ...