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Citations for Addgene :
1 - 50 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... N-terminal FLAG-tagged CAPS was created by recombining the human CAPS gene (CAPS/pENTR; DNAsu HscD00514950) into pEZFLAG (Addgene#18700; Guo et al., 2008) using Gateway cloning ...
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bioRxiv - Pathology 2024Quote: ... pR26 CAG/GFP Asc was a gift from Ralf Kuehn (Addgene plasmid # 74285). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: sgRNAs were ordered as oligonucleotides from Sigma-Aldrich and inserted into the PX330 CRISPR vector (Addgene 42230) by digestion with BbsI ...
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bioRxiv - Cancer Biology 2024Quote: ... pLenti-Lifeact-tdTomato (RRID: Addgene_64048)
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bioRxiv - Cancer Biology 2024Quote: ... pCDH-EF1a-mCherry-EGFP-LC3B (RRID: Addgene_170446); pLenti-Lifeact-tdTomato (RRID ...
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bioRxiv - Cell Biology 2024Quote: ... The ER-Halo and the ER-GCaMP6-150 (Addgene #86918) under a CamKII promoter were both restriction digested with AgeI and the HALO protein was ligated with Quick Ligase (NEB ...
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bioRxiv - Plant Biology 2024Quote: ... digested vector pKS diaCas9_sgRNA (Addgene #74923). The homology donor plasmid was constructed via Gibson assembly (ThermoFisher GeneArt ...
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bioRxiv - Neuroscience 2024Quote: ... V2.0 was a gift from Feng Zhang (Broad Institute, Boston, USA) (Addgene plasmid # 62988; http://n2t.net/addgene:62988; RRID:Addgene_62988). Transient transfection of PX459 into OPs was conducted using Lipofectamine 3000 (Thermo Fisher) ...
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bioRxiv - Plant Biology 2024Quote: ... TurboID was amplified from Addgene plasmid #127350 (Mair et al. ...
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bioRxiv - Neuroscience 2024Quote: ... to express the genetically encoded calcium indicator GCaMP under the pan-neuronal synapsin promoter (AAV1-Syn-jGCaMP7f- WPRE, AddGene, viral prep 104488-AAV1, titer 2x1012). The needle was left in place for 10 min to allow for virus diffusion.
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-mDlx-GFP-Fishell-1 (a gift from Gordon Fishell, Addgene plasmid # 83900; http://n2t.net/addgene:83900; RRID:Addgene_83900) by restriction subcloning ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-mDlx-GFP-Fishell-1 (a gift from Gordon Fishell, Addgene plasmid # 83900 ...
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bioRxiv - Neuroscience 2024Quote: pAAV-mDlx-eGFP-ATG5 was generated from pEGFP-C1-hApg5 (a gift from Noboru Mizushima, Addgene plasmid # 22952) and pAAV-mDlx-GFP-Fishell-1 (a gift from Gordon Fishell ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV2/rh10 (Addgene plasmid # 112866 ...
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bioRxiv - Physiology 2024Quote: ... (1.3 × 1011 plaque-forming units per mouse, ((107787-AAV8, Addgene, Watertown, MA)) ...
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bioRxiv - Physiology 2024Quote: ... 200 ng pDisplay-gGRAB5HT2m-IRES-mCherryCAAX (Addgene, #208710), 200 ng pcDNA3-5-HT2A-P2A-GCaMP8m ...
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bioRxiv - Physiology 2024Quote: ... or 200 ng pDisplay-ATP1.0-IRES-mCherryCAAX (Addgene, #167582) was transfected to HEK293T cells in 24-well plates ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV2/rh10 (Addgene plasmid # 112866; http://n2t.net/addgene:112866; RRID:Addgene_112866) were a gift from James M ...
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bioRxiv - Neuroscience 2024Quote: Calcium indicator GCaMP6f (AAV1-Synapsin-GCaMP6f-WPRE-SV40, Addgene 100837-AAV1; 6.52 ×1012GC/ml) was injected using the same procedure at 4.5-5 months after injection of AAV-GFAP-htTau or AAV-GFAP-Control ...
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bioRxiv - Neuroscience 2024Quote: ... The plasmids pAdDeltaF6 (Addgene plasmid #112867; http://n2t.net/addgene:112867; RRID:Addgene_112867) and pAAV2/rh10 (Addgene plasmid # 112866 ...
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bioRxiv - Neuroscience 2024Quote: The pCAGGS-IRES-TdTomato construct was obtained from Addgene (#83029). pCAGGS-IRES- Cre-TdTomato was generated in the lab ...
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bioRxiv - Neuroscience 2024Quote: ... The mCherry plasmid was obtained from Addgene (#84329) (van Unen et al. ...
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bioRxiv - Neuroscience 2024Quote: ... To target astrocytes with chemogenetics we injected 0.60 µl bilaterally of the AAV5-GFAP-hM3D(Gq)-mCherry (Addgene 50478) (viral titer 2×1013 vg per ml ...
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bioRxiv - Neuroscience 2024Quote: ... was used to inject 300 nl of either a non-specific GtACR2 (pAAV1-CamKIIa-stGtACR2-FusionRed, Addgene #105669), or a Cre-dependent GtACR2 (pAAV1-hSyn1-SIO-stGtACR2-FusionRed ...
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bioRxiv - Physiology 2024Quote: ... or pcDNA3.1-PPARgamma2 (#78768, Addgene, Watertown, MA, USA) plasmids using Lipofectamine 3000 (ThermoFisher Scientific) ...
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bioRxiv - Physiology 2024Quote: ... were obtained from Addgene. pAAV7-DJ-CMV-TeNT-P2A-GFP was prepared and obtained through the Stanford Viral Core ...
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bioRxiv - Physiology 2024Quote: pAAV-hSyn-mCherry (Addgene #114472-AAV8) and pAAV-hSyn-hM3D(Gq)-mCherry (Addgene #50474-AAV8 ...
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bioRxiv - Physiology 2024Quote: ... and pAAV-hSyn-hM3D(Gq)-mCherry (Addgene #50474-AAV8) were obtained from Addgene ...
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bioRxiv - Physiology 2024Quote: ... Repair templates in pDD282 (Addgene 66823) (forward primer for upstream arm ...
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bioRxiv - Physiology 2024Quote: AAV9 virus expressing jGCaMP8m under a CAG promoter was obtained from Addgene (162381-AAV9). For injections ...
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bioRxiv - Plant Biology 2024Quote: ... together with a fragment containing the nucleotides encoding for the Pip1 pro-domain but lacking the signal peptide (pJK110; Supplemental Table S4) were combined in all 64 possible combinations with pICH41264 (Addgene #4799; Weber et al., 2011) in a BpiI Golden Gate reaction (Supplemental Table S4) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The plasmid backbone pAM552G (35, 47) (Addgene accession #154131) was used for testing ribosome variants from RISE in vivo (see below) ...
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bioRxiv - Synthetic Biology 2024Quote: ... pJL1sfGFP (encoding the reporter sfGFP under a T7 promoter, Addgene accession #102634) and pRDV3xFLAG-TolA-HH (encoding the 3xFLAG tag for ribosome selection under a T7 promoter ...
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bioRxiv - Synthetic Biology 2024Quote: A plasmid containing GCaMP6m was obtained from Addgene (36) and gblocks were obtained from IDT ...
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bioRxiv - Neuroscience 2024Quote: ... Virus pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (Addgene viral prep #1120050-AAV5) was injected into layer 2/3 of the primary somatosensory barrel (S1BF ...
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bioRxiv - Neuroscience 2024Quote: ... To monitor astrocytic Ca2+ signaling we used the genetically encoded Ca2+ indicator (GECI) AAV5-gfaABC1D-cyto-GCaMP6f (Addgene 52925) (viral injections of 0.6 µl ...
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bioRxiv - Neuroscience 2024Quote: ... Lenti-Cas9 encoding Cas9 was produced from Addgene plasmid #87904 ...
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bioRxiv - Neuroscience 2024Quote: ... GABAergic interneuron targeting was achieved with 0.45 µl of AAV1-1/2mDlx-HBB-hChR2-mcherry (Addgene 83898) (viral titer 7×1012 vg per ml ...
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bioRxiv - Neuroscience 2024Quote: ... and Cre dependent eYFP plasmid (pSin wPGK-Cre; Addgene plasmid # 101242 and pFUdioeYFPW; Addgene plasmid # 73858, respectively) has been previously described(Chen et al. ...
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bioRxiv - Neuroscience 2024Quote: ... The dLight virus (700 nL of AAV5-hSyn-dLight 1.2; Addgene, Watertown, MA; Catalog No.111068-AAV5) was injected unilaterally into the NAc core (in mm ...
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bioRxiv - Neuroscience 2024Quote: ... pSPAX2 and pMD2.G (Addgene plasmids 12260 and 12259, respectively) were transfected using Lipofectamine 3000 (ThermoFisher ...
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bioRxiv - Pathology 2024Quote: ... (WT and variants) with a mTurquoise (mTq2) tag on the C-terminus were cloned into a pSBtet-pur vector (Addgene) using SfiI sites ...
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bioRxiv - Neuroscience 2024Quote: ... and Rab10T23N were purchased from Addgene (ER-GCaMP6-150 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... a hNav1.5 plasmid (Addgene plasmid #145374 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The CLYBL-targeting pC13N-iCAG.copGFP vector (Addgene #66578) [66] was used as a backbone to generate three constructs encoding different N-terminal-HiBiT-GBA1 variants (WT ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... along with left and right TALENs (pZT-C13-L1: Addgene #62196; pZT-C13-R1: Addgene #62197; 375 ng/well each) targeting the human Citrate Lyase Beta-Like (CLYBL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... along with left and right TALENs (pZT-C13-L1: Addgene #62196 ...
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bioRxiv - Neuroscience 2024Quote: ... or a Cre-dependent GtACR2 (pAAV1-hSyn1-SIO-stGtACR2-FusionRed, Addgene #105677) into each burr hole approximately 0.5 mm below the pial surface with an injection rate of 10-20 nl/min ...
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bioRxiv - Synthetic Biology 2024Quote: ... Mutations were introduced using the same primers on BcLOV4-ITSN1 (Provided by Brian Chow) (Addgene #174509) to generate meltITSN1-37 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Melt mutations were introduced to WT BcLOV4 (Provided by Brian Chow) (Addgene Plasmid #114595) via whole backbone PCR using primers containing the target mutation ...