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Citations for Addgene :
2351 - 2400 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... They were incorporated into lentiCRISPR-v2 (Addgene, Cat# 52961) following the GeCKO protocol with minor adjustments(Sanjana et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... and packaging plasmid (psPAX2) (Addgene, 12260) using Lipofectamine 3000 per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and the FLP recombinase expression plasmid (pFLP3, Addgene plasmid # 64946) were gifts from Herbert Schweizer [38].
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bioRxiv - Microbiology 2024Quote: ... Tn7 transposase expression plasmid (pTNS, Addgene plasmid # 64967), and the FLP recombinase expression plasmid (pFLP3 ...
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bioRxiv - Molecular Biology 2024Quote: ... pcDNA3.1-FLAG-gp130:sarA chimera codon-optimized for mammalian expression were previously described in and available from Addgene. Site-directed mutagenesis was carried out using Quikchange Lightning Site-Directed Mutagenesis Kit (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of CB1-GFP and 100 ng of Rab5-BFP (Addgene #49147) were mixed with 0.2 µL of lipofectamine 2000 following transfection method previously described then added to the wells ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of the EPAC sensor (Addgene #61622) were mixed with 0.2 µL of lipofectamine 2000 following the transfection method previously described ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 ng of hMbPylRS-4xU6M15 (Addgene, #105830) and 200 ng of the respective amber construct were premixed in 20 µL of DMEM ...
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bioRxiv - Systems Biology 2024Quote: ... coli plasmid-propagating strains from Addgene, and are a generous gift from the Roth laboratory by way of Addgene [69] ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-Syn-FLEX-GCaMP6s-WPRE-SV40 was developed from Douglas Kim & GENIE Project (Chen et al., 213) (Penn Vector Core or 100845-AAV1, Addgene). rAAV2-Retro–Ef1α-DIO-SwiChR++-eYFP (R47730 ...
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bioRxiv - Neuroscience 2024Quote: ... pGP-AAV-Syn-FLEX-jGCaMP8s-WPRE was developed from the GENIE Project (Zhang et al., 2023) (162377-AAVrg, Addgene). pAAV-Syn-FLEX-GCaMP6s-WPRE-SV40 was developed from Douglas Kim & GENIE Project (Chen et al. ...
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bioRxiv - Systems Biology 2024Quote: ... The Activator reporter construct (Addgene, this study) contains the PuroR-IRES-GFP reporter under the control of the minimal promoter derived from the MYLPF gene (chr16:30374730−30374857 + ...
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bioRxiv - Systems Biology 2024Quote: ... The PTGR reporter construct (Addgene, this study) encodes for PuroR-IRES-GFP followed by 10 boxB sites that are flanked by two loxP sites under the control of a PGK promoter ...
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bioRxiv - Systems Biology 2024Quote: ... each promoter was transferred to the pMW#2 (Addgene #13349) and pMW#3 (Addgene #13350 ...
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bioRxiv - Systems Biology 2024Quote: ... or rabbit α-β-tubulin (Addgene ab6046, 1:10,000) as primary and HRP-α-Mouse (Cell Signaling ...
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bioRxiv - Microbiology 2024Quote: ... pcDNA3.1-Ha-Dynamin 2.K44A (gift of S. Schmid, Addgene 34685), pEGFPC1 (Clontech) ...
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bioRxiv - Microbiology 2024Quote: ... pEGFP-Dynamin 2.K44A (gift of P. De Camilli; Addgene 22301), and pEGFP-Dynamin 2ΔPRD (50 ...
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bioRxiv - Microbiology 2024Quote: Mammalian expression vectors used were pcDNA3.1-Ha-Dynamin 2.WT (gift of S. Schmid; Addgene 34684), pcDNA3.1-Ha-Dynamin 2.K44A (gift of S ...
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bioRxiv - Cell Biology 2024Quote: The pLKO.1 – TRC cloning vector was a gift from David Root (Addgene plasmid # 10878 ...
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bioRxiv - Cell Biology 2024Quote: ... Lentivirus packaging (psPAX2, #12260) and envelope (pMD2.G, #12259) plasmids were purchased from Addgene.
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of CB1-GFP and 100 ng of R-GECO (Addgene #32444) were mixed with 0.2 µL of lipofectamine 2000 transfection reagent ...
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bioRxiv - Neuroscience 2024Quote: ... annealed and cloned into the pSpCas9(BB)-2A-GFP (pX458) vector (Addgene), and constructs were then transfected into tsA201 cells with Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... The mini-Tn7 vector (pUC18T-mini-Tn7T-GM, Addgene plasmid # 63121), Tn7 transposase expression plasmid (pTNS ...
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bioRxiv - Microbiology 2024Quote: ... were produced in HEK293T cells along with the VSV-G (Addgene plasmid # 8454) and ΔR8.2 (Addgene plasmid # 8455 ...
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bioRxiv - Microbiology 2024Quote: The full-length HIV vectors NL4-3 ΔEnv EGFP (HIV Reagent Program) and HIVGKO (Addgene plasmid #112234) were produced in HEK293T cells along with the VSV-G (Addgene plasmid # 8454 ...
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bioRxiv - Cancer Biology 2024Quote: ... 466 sgRNAs were selected from the H3 knock out library (Addgene # 133914) from Brown/Liu labs.
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bioRxiv - Cancer Biology 2024Quote: The pCC_01 – hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE plasmid (Addgene #139086) was used for CRISPR screen and validation ...
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bioRxiv - Cancer Biology 2024Quote: pCC_01 and pCC_09 (Addgene #139094) were modified to incorporate Capture Sequences 1 and 2 at the 3’ end of guide sequences ...
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bioRxiv - Microbiology 2024Quote: ... envelope protein-expressing vector pCMV-VSVG (38) and the transfer vectors pEF1a-CAS9-2A-Blasticidin (#52962, Addgene) or pU6-gRNA-PGK-Puro-2A-BFP encoding CRISPR-Cas9 guide RNAs (gRNAs ...
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bioRxiv - Cancer Biology 2024Quote: ... WT and mutant constructs were subsequently cloned into the vector pLenti CMV Neo DEST (705-1) (Addgene, #17392) by the GATEWAY cloning system ...
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bioRxiv - Cancer Biology 2024Quote: ... The DNA Damage Response MKOv4 Library (Addgene, #140219) was from our previous study35.
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bioRxiv - Microbiology 2024Quote: ... and ΔR8.2 (Addgene plasmid # 8455) plasmids for lentivirus generation ...
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bioRxiv - Cancer Biology 2024Quote: ... shp53 pLKO.1 puro was purchased from Addgene (19119) (shp53 pLKO.1 puro was a gift from Bob Weinberg (Addgene plasmid # 19119 ...
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bioRxiv - Cancer Biology 2024Quote: ... shp53 pLKO.1 puro was purchased from Addgene (19119) (shp53 pLKO.1 puro was a gift from Bob Weinberg (Addgene plasmid # 19119 ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... plasmids of interest (see plasmids section) were co-transfected with psPAX.2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... and pMD2.G (Addgene #12259) plasmids with a ratio of 3:2:1 by the calcium phosphate method into HEK 293T cells to package lentiviral particles ...
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bioRxiv - Microbiology 2024Quote: ... gRNAs were cloned into the pU6-Cas9 Universal Plasmid (Addgene, 52694) using BsaI ...
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bioRxiv - Plant Biology 2024Quote: ... pRET FlipSuc-90u delta1 was a gift from Wolf Frommer (Addgene plasmid #14475; http://n2t.net/addgene:14475; RRID: Addgene_14475). To measure sucrose uptake of AtSWEET13 wildtype and variants ...
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bioRxiv - Plant Biology 2024Quote: ... and finally cloned into the Level1 pICH47732 plasmid (Addgene catalog # 48000) with a CaMV 35S short promoter (pICH51277 ...
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bioRxiv - Plant Biology 2024Quote: ... pRET FlipSuc-90u delta1 was a gift from Wolf Frommer (Addgene plasmid #14475 ...
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bioRxiv - Synthetic Biology 2024Quote: ... dCas9 was originally amplified from plasmid pJMP1159 (supplied by Addgene) removing the myc tag (Peters et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... each well was transfected with 380 ng psPAX2 (Addgene Plasmid #12260), 140 ng pMD2.G (Addgene Plasmid #12259) ...
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bioRxiv - Synthetic Biology 2024Quote: ... cloned from pCE048-SiT-Cas12a (Addgene #128124), between coding sequences and pre-crRNA motif ...
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bioRxiv - Synthetic Biology 2024Quote: ... hgRNA-E21_pLKO-Hyg (Addgene Plasmid #100563), and hgRNA-A21_pLKO-Hyg (Addgene Plasmid # 100559 ...
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bioRxiv - Synthetic Biology 2024Quote: ... hgRNA-D21_pLKO-Hyg (Addgene Plasmid #100562), hgRNA-E21_pLKO-Hyg (Addgene Plasmid #100563) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and then merged it into the 3’UTR of eGFP expression cascade in LPutopia-7 (Addgene #199212) plasmid ...
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bioRxiv - Synthetic Biology 2024Quote: ... in the previous cloned pUt-mNF-GFP RMCE plasmid28 (Addgene #199220). Then MONARCH 1.0 circuit was established by inserting pre-crRNA motif (with GFP-targeting crRNA ...
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bioRxiv - Synthetic Biology 2024Quote: ... and hgRNA-A21_pLKO-Hyg (Addgene Plasmid # 100559) were ordered from Addgene6 ...
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bioRxiv - Synthetic Biology 2024Quote: ... pcDNA-Cas9-T2A-TdT (Addgene Plasmid #126424) and pcDNA-Cas9-T2A-STOP-TdT (Addgene Plasmid #126425 ...
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bioRxiv - Synthetic Biology 2024Quote: The AAVS1-targeting eSpCas9 vector (Addgene #199213) was constructed in the previous study28 ...