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Citations for Addgene :
2351 - 2400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... the BMP2 CDS was removed and replaced with EGFP excised from the pCAG_GFP plasmid previously purchased from Addgene (https://www.addgene.org/11150/). Novel SP fragment synthesis ...
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bioRxiv - Synthetic Biology 2024Quote: ... genome using a previously reported phage-recombinase method.23 Genetic sensor constructs were first constructed on the shuttle plasmid pTRKH2 (Addgene Plasmid #71312) and sequence verified ...
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bioRxiv - Synthetic Biology 2024Quote: ... pJFRC81-10XUAS:IVS-Syn21-GFP-p10 (hereafter pJFRC81) was a gift from Gerald Rubin (Addgene plasmid #36432 ; http://n2t.net/addgene:36432 ; RRID:Addgene_36432). The region 67 bp upstream of the start codon of the GFP CDS of pJFRC81 was used as an extended upstream homology arm to re-introduce the Syn21 5’UTR during assembly ...
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bioRxiv - Systems Biology 2024Quote: ... CYC1t and pTEF1 were both PCR amplified from BB3cK_pGAP_23*_pTEF_Cas9 (Addgene #104909) (Gassler et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the sgRNA for the TREE reporter system (164413) were all obtained from Addgene. To generate Ai9 reporter 293T cells ...
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bioRxiv - Synthetic Biology 2024Quote: ... pJFRC81-10XUAS:IVS-Syn21-GFP-p10 (hereafter pJFRC81) was a gift from Gerald Rubin (Addgene plasmid #36432 ...
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bioRxiv - Systems Biology 2024Quote: ... pEN_TT CVB3 A67G (Addgene, 216788) was prepared in a two-step procedure ...
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bioRxiv - Synthetic Biology 2024Quote: ... Select regions of EphA1 (excluding the extracellular regions of IM-EphA1Cry2 construct) were cloned into Cry2olig-mCherry (Addgene: #60032) using NheI and BmtI restriction sites ...
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bioRxiv - Synthetic Biology 2024Quote: ERK-KTR-mNeonGreen was subcloned from Addgene plasmid #129631 (Chavez-Abiega ...
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bioRxiv - Systems Biology 2024Quote: ... sgRNAs were cloned into the CROPseq-puro-v2 backbone (Addgene #127458). pXPR_BRD023 (lentiCRISPR v2 ...
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bioRxiv - Systems Biology 2024Quote: ... BJ1 cells were transduced with Cas9 (Addgene #96924) and selected using 10 µg/mL blasticidin for 7 days ...
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bioRxiv - Systems Biology 2024Quote: Libraries were cloned as previously described into a CROP-seq-puro-v2 (Addgene #127458) backbone ...
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bioRxiv - Synthetic Biology 2024Quote: ... the two fragments of FlipGFP B1-9 and B10-E5-B11-TEVcs-K5 were amplified from Addgene Plasmid #124429 via PCR ...
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bioRxiv - Synthetic Biology 2024Quote: ... pCMV-dR8.91 (Addgene, catalog number 12263), and pMD2.G (Addgene ...
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bioRxiv - Synthetic Biology 2024Quote: ... and pMD2.G (Addgene, catalog number 12259) into Lenti-X HEK293T ...
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bioRxiv - Synthetic Biology 2024Quote: ... CPxx synthetic promoters and RBS were either amplified from plasmids acquired from Addgene, from our stocks ...
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bioRxiv - Synthetic Biology 2024Quote: ... The construction pUCmini-iCAP-PHP.S for AAV-PHP.S serotype assembly was kindly gifted by Viviana Gradinaru (Addgene plasmid #103006). For targeted expression of constructs in cardiomyocytes ...
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bioRxiv - Synthetic Biology 2024Quote: ... TNF (pLI_TNF, which was a gift from Veit Hornung, Addgene plasmid #171179)29 ...
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bioRxiv - Synthetic Biology 2024Quote: Ligands were cloned into a pcDNA backbone to confer high expression in HEK293FT cells (Addgene #138749)26 ...
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bioRxiv - Synthetic Biology 2024Quote: ... IL-10 (pHR_Gal4UAS_humanIL-10_T2A_PDL1_PGK_mCherry, which was a gift from Wendell Lim, Addgene plasmid #85430)9 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Genes encoding natural transcription factors were sourced from: cJun (pCLXSN-c-JUN, which was a gift from Jin Chen, Addgene plasmid #102758)36 and BATF (pFUW-TetO-BATF ...
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bioRxiv - Synthetic Biology 2024Quote: ... which was a gift from Ralf Kuehn (Addgene plasmid #74285)33 ...
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bioRxiv - Synthetic Biology 2024Quote: ... the sequences encoding for LgBiT and SpyCatcher003 were amplified from the original LgBiT plasmid and sCatch-GFP plasmid (Addgene #18690241), respectively ...
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bioRxiv - Synthetic Biology 2024Quote: ... and TGF-β1 (TGFB1_pLX307, which was a gift from William Hahn and Sefi Rosenbluh, Addgene plasmid #98377)30 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Addgene plasmid #102758)36 and BATF (pFUW-TetO-BATF, which was a gift from Filipe Pereira, Addgene plasmid #178451)37 ...
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bioRxiv - Synthetic Biology 2024Quote: ... which was a gift from Michael Elowitz (Addgene plasmid #78099)32 ...
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bioRxiv - Synthetic Biology 2024Quote: ... which was a gift from Feng Zhang (Addgene plasmid #61425)35 ...
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bioRxiv - Synthetic Biology 2024Quote: ... which was modified by our laboratory in previous work (pPD005, Addgene #138749)26 and further modified here to change the antibiotic resistance marker from ampicillin to kanamycin and to add the additional expression cassette containing a CMV promoter ...
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bioRxiv - Systems Biology 2024Quote: ... 1.5 μg of pMD2.G (Addgene #12259) and 18 μl of 2 mg/ml PEI 25000 was mixed in Opti-MEM (Gibco #31985062 ...
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bioRxiv - Systems Biology 2024Quote: ... was created by PCR cloning of 3xHA-MKK6-EE (Addgene, 47579) with BglII–SalI ends and inserting into pBABE puro (Addgene ...
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bioRxiv - Systems Biology 2024Quote: ... pBABE 3xHA-MKK6-EE puro (Addgene, 216791) was created by PCR cloning of 3xHA-MKK6-EE (Addgene ...
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bioRxiv - Systems Biology 2024Quote: ... and cloned into lentiGuide-Puro (Addgene 52963) by the Golden Gate method as described in (https://media.addgene.org/cms/filer_public/3e/e1/3ee1ce9c-99f9-4074-9a28-109d34971471/zhang-lab-sam-cloning-protocol.pdf) ...
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bioRxiv - Systems Biology 2024Quote: ... and pSLIK CVB3 A67G hygro are BSL2 plasmids that cannot be distributed by Addgene and therefore are available upon request.
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bioRxiv - Systems Biology 2024Quote: We established a stable Cas9-expressing line by infecting EpiSC-5 cells with lentiCas9- Blast (Addgene 52962), followed by selection with 5 µg/ml blasticidin (Sigma 15205 ...
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bioRxiv - Systems Biology 2024Quote: pBABE FLAG-IKK2-EE puro (Addgene, 216790) was created by PCR cloning of IKK2-EE (Addgene ...
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bioRxiv - Systems Biology 2024Quote: ... with BclI–MfeI ends and inserting into pBABE puro (Addgene, 1764) digested with BamHI–EcoRI ...
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bioRxiv - Systems Biology 2024Quote: ... with BglII–SalI ends and inserting into pBABE puro (Addgene, 1764) digested with BamHI–SalI ...
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bioRxiv - Neuroscience 2024Quote: Following plasmids were used for transfection pN1-CMV-sDarken (Addgene plasmid #184799 ...
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bioRxiv - Neuroscience 2024Quote: Following plasmids were used for transfection pN1-CMV-sDarken (Addgene plasmid #184799, pN1-CMV-L-sDarken (Addgene plasmid #184800), null-mutant of sDarken (created in house) ...
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bioRxiv - Neuroscience 2024Quote: ... MARK3-V5 (Addgene, 107235), shRNA control (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The oligo pool containing the sgRNA sequences was synthesized by Genewiz (Suzhou, Jiangsu, China) and cloned into lenti-guide-puro (#52963; Addgene, Watertown, MA, USA) as previously described51 ...
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bioRxiv - Synthetic Biology 2024Quote: ... these three fragments and the pTU1 backbone (Addgene #72934) were ligated to form G363.
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bioRxiv - Neuroscience 2024Quote: ... all mice were unilaterally injected with 800nL of AAV9-syn-GCaMP8m-WPRE at a titer of 1.2e12 (Addgene virus #162375) in the RSC ...
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bioRxiv - Synthetic Biology 2024Quote: ... which was a kind gift of Sander Tans (Addgene #195090). The genomic deletion was achieved by following the λRed recombination protocol [75] ...
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bioRxiv - Synthetic Biology 2024Quote: ... which were kind gifts from Johan Paulsson (Addgene plasmid #85525 and #85524). To construct pJP Rep00 ...
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bioRxiv - Cancer Biology 2024Quote: ... pREV (Addgene #115989), and pMD2.g (Addgene #12259 ...
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bioRxiv - Cancer Biology 2024Quote: ... Guide RNAs (gRNAs) were cloned into the pSpCas9n(BB)-2A-GFP (PX461) plasmid (Addgene #48140) and delivered to parental K562 cells by Amaxa nucleofection using the Amaxa Cell line nucleofector kit V (Lonza VCA-1003) ...
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bioRxiv - Cancer Biology 2024Quote: High-titre lentiviral supernatants were produced by transient transfection of 293T/17 cells with second-generation packaging/envelope vectors pRRE (Addgene #12251), pREV (Addgene #115989) ...
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bioRxiv - Neuroscience 2024Quote: pGP-AAV-syn-jGCaMP7c variant 1513-WPRE was a gift from Douglas Kim & GENIE Project (Addgene viral prep # 105321-AAV1 ...
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bioRxiv - Neuroscience 2024Quote: ... A 603 bp long nucleotide encoding for cystatin-7 was cloned into the BamHI/EcoRI sites of pAAV-hSyn-EGFP (# 50465, Addgene) to receive the plasmid pAAV-hSyn-Cst7 ...