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• A whole-genome CRISPR screen identifies the spindle accessory checkpoint as a locus of nab-paclitaxel resistance in pancreatic cancer cells

  Priya Mondal, et al., bioRxiv - Cancer Biology 2024

  Quote: The human CRISPR Brunello lentiviral pooled library (Addgene # 73178-LV)14 and human CRISPR Dolcetto (Set A) inhibition library (Addgene # 92386-LV)15 were used to identify genes responsible for enhanced survival of PANC-1 cells treated with nab-paclitaxel ...
• A whole-genome CRISPR screen identifies the spindle accessory checkpoint as a locus of nab-paclitaxel resistance in pancreatic cancer cells

  Priya Mondal, et al., bioRxiv - Cancer Biology 2024

  Quote: The human CRISPR Brunello lentiviral pooled library (Addgene # 73178-LV)14 and human CRISPR Dolcetto (Set A ...
• Gamma protocadherins in vascular endothelial cells inhibit Klf2/4 to promote atherosclerosis

  Divyesh Joshi, et al., bioRxiv - Cell Biology 2024

  Quote: ... and psPAX2 (Addgene, #12260) packaging plasmids using lipofectamine 2000 (ThermoFisher ...
• Constitutive Plasma Membrane Turnover in T-REx293 cells via Ordered Membrane Domain Endocytosis under Mitochondrial Control

  Christine Deisl, et al., bioRxiv - Cell Biology 2024

  Quote: ... The T-REx293 cell line was transiently transfected with LentiCRISPRv2 (Addgene, #52961). The sgRNA sequences used for cloning LentiCRISPRv2-DHHC5 were ...
• Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle

  Xiaodong Wang, et al., bioRxiv - Cell Biology 2024

  Quote: ... the full-length coding sequence (CDS) of CREB was amplified and inserted into the pcDNA3.1 plasmid (Addgene, USA). HEK293T cells were seeded in a 24-well plate and incubated for 24 hours ...
• Selective Translation Orchestrates Key Signaling Pathways in Primed Pluripotency

  Chikako Okubo, et al., bioRxiv - Cell Biology 2024

  Quote: ... along with 3.75 μg of psPAX2 (courtesy of Didier Trono: Addgene, #12260) and 1.25 μg of pMD2.G (courtesy of Didier Trono ...
• Identification of a motif in TPX2 that regulates spindle architecture in Xenopus egg extracts

  Guadalupe E. Pena, et al., bioRxiv - Cell Biology 2024

  Quote: ... tropicalis proteins were cloned into the pHAT2 vector (Addgene #112583) using NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621) ...
• Combinatorial in vivo genome editing identifies widespread epistasis during lung tumorigenesis

  Jess D. Hebert, et al., bioRxiv - Cancer Biology 2024

  Quote: ... envelope plasmid and pCMV-dR8.2 dvpr (Addgene #8455) packaging plasmid using polyethylenimine (Polysciences) ...
• The Cyclin-Like Protein Spy1 Mediates Tumourigenic Potential of Triple Negative Breast Cancer

  Bre-Anne Fifield, et al., bioRxiv - Cancer Biology 2024

  Quote: ... DHB-mVenus (CDK2 activity reporter) was a gift from Tobias Meyer & Sabrina Spencer (Addgene plasmid # 136461; http://n2t.net/addgene:136461; RRID:Addgene_136461) (27).
• GPX3 supports ovarian cancer tumor progression in vivo and promotes expression of GDF15

  Caroline Chang, et al., bioRxiv - Cancer Biology 2024

  Quote: ... or an empty backbone pLKO.1 control (Addgene plasmid #8453) were used to generate virus and infect OVCAR3 cells or ID8 cells ...
• Mechanical confinement governs phenotypic plasticity in melanoma

  Miranda V. Hunter, et al., bioRxiv - Cancer Biology 2024

  Quote: ... The FastFUCCI reporter plasmid was obtained from Addgene (#86849). The HMGB2-GFP reporter plasmid was generated as described above ...
• Timing of treatment shapes the path to androgen receptor signaling inhibitor resistance in prostate cancer

  Eugine Lee, et al., bioRxiv - Cancer Biology 2024

  Quote: Pre-Intact clone was infected with pLV-Azurite (blue fluorescent protein, Addgene, 36086) and Pre-ARSIR1 clone was infected with SGEP-Renilla-713 (GFP ...
• Targeting PIKfyve-driven lipid homeostasis as a metabolic vulnerability in pancreatic cancer

  Caleb Cheng, et al., bioRxiv - Cancer Biology 2024

  Quote: The Human CRISPR Metabolic Gene Knockout library was a gift from David Sabatini (Addgene #110066)78 ...
• Targeting PIKfyve-driven lipid homeostasis as a metabolic vulnerability in pancreatic cancer

  Caleb Cheng, et al., bioRxiv - Cancer Biology 2024

  Quote: ... pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro (Addgene plasmid # 71236 ...
• Targeting PIKfyve-driven lipid homeostasis as a metabolic vulnerability in pancreatic cancer

  Caleb Cheng, et al., bioRxiv - Cancer Biology 2024

  Quote: ... pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro (Addgene plasmid # 71236; http://n2t.net/addgene:71236; RRID: Addgene_71236)91 using the Golden Gate reaction ...
• Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing

  Svenja K. Tetzlaff, et al., bioRxiv - Cancer Biology 2024

  Quote: ... RabV CVS-N2c(deltaG)-EGFP was a gift from Thomas Jessell (Addgene plasmid #73461; http://n2t.net/addgene:73461; RRID: Addgene_73461). pSADdeltaG-F3 was a gift from Edward Callaway (Addgene plasmid #32634 ...
• Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing

  Svenja K. Tetzlaff, et al., bioRxiv - Cancer Biology 2024

  Quote: ... RabV CVS-N2c(deltaG)-EGFP was a gift from Thomas Jessell (Addgene plasmid #73461 ...
• Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing

  Svenja K. Tetzlaff, et al., bioRxiv - Cancer Biology 2024

  Quote: DIV06 rat cortical neurons were infected with AAV5 virus based on the AAV-Flex-TACasp3-TEVP plasmid (Addgene #45580)83 at a titer of >7x108 vg/ml ...
• Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing

  Svenja K. Tetzlaff, et al., bioRxiv - Cancer Biology 2024

  Quote: ... cultures were infected with AAV.Syn.NES-jRGECO1a.WPRE.SV40 (Addgene #100854).125 AAV.Syn.NES-jRGECO1a.WPRE.SV40 was a gift from Douglas Kim & GENIE Project (Addgene plasmid #100854 ...
• Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing

  Svenja K. Tetzlaff, et al., bioRxiv - Cancer Biology 2024

  Quote: ... pSADdeltaG-F3 was a gift from Edward Callaway (Addgene plasmid #32634 ...
• Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing

  Svenja K. Tetzlaff, et al., bioRxiv - Cancer Biology 2024

  Quote: ... AAV-flex-taCasp3-TEVp was a gift from Nirao Shah & Jim Wells (Addgene plasmid #45580 ...
• Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing

  Svenja K. Tetzlaff, et al., bioRxiv - Cancer Biology 2024

  Quote: ... DIV6 rat cortical neurons were treated with AAV-mDlx-NLS-mRuby (Addgene #99130) at a titer of >1x109 vg/ml.121 AAV-mDlx-NLS-mRuby2 was a gift from Viviana Gradinaru (Addgene plasmid #99130 ...
• Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing

  Svenja K. Tetzlaff, et al., bioRxiv - Cancer Biology 2024

  Quote: ... or SAD-B19ΔG-eGFP (modified from Addgene plasmid # 32634). Supernatant was collected over several days and the recovered virus was re-transfected in B7GG cells for a final collection step ...
• Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing

  Svenja K. Tetzlaff, et al., bioRxiv - Cancer Biology 2024

  Quote: ... at a titer of >1x109 vg/ml.121 AAV-mDlx-NLS-mRuby2 was a gift from Viviana Gradinaru (Addgene plasmid #99130; http://n2t.net/addgene:99130; RRID: Addgene_99130). One day later ...
• Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing

  Svenja K. Tetzlaff, et al., bioRxiv - Cancer Biology 2024

  Quote: ... pSADdeltaG-F3 was a gift from Edward Callaway (Addgene plasmid #32634; http://n2t.net/addgene:32634; RRID: Addgene_32634).
• Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing

  Svenja K. Tetzlaff, et al., bioRxiv - Cancer Biology 2024

  Quote: ... at a titer of >1x109 vg/ml.121 AAV-mDlx-NLS-mRuby2 was a gift from Viviana Gradinaru (Addgene plasmid #99130 ...
• Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing

  Svenja K. Tetzlaff, et al., bioRxiv - Cancer Biology 2024

  Quote: ... SAD-B19ΔG-Cre-GFP(EnVA) (based on Addgene plasmid #32634) was added at a titer of 104 vg/ml.112 Control wells were treated with the same concentration of SAD-B19ΔG-Cre-GFP(EnVA ...
• Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing

  Svenja K. Tetzlaff, et al., bioRxiv - Cancer Biology 2024

  Quote: ... AAV-flex-taCasp3-TEVp was a gift from Nirao Shah & Jim Wells (Addgene plasmid #45580; http://n2t.net/addgene:45580; RRID: Addgene_45580). The following day ...
• Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing

  Svenja K. Tetzlaff, et al., bioRxiv - Cancer Biology 2024

  Quote: ... B7GG cells were transfected by Lipofectamine 3000 (Thermo Fischer) with rabies virus genomic vectors RabV CVS-N2cΔG-eGFP (Addgene plasmid #73461) or SAD-B19ΔG-eGFP (modified from Addgene plasmid # 32634) ...
• Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing

  Svenja K. Tetzlaff, et al., bioRxiv - Cancer Biology 2024

  Quote: ... TVA-oG-mCherry expressing glioblastoma spheroids were transduced with rabies virus constructs SAD-B19ΔG- eGFP(EnVA) or CVS-N2cΔG-eGFP(EnVA) (Addgene #73461) prior to further experiments.33 Transduced cells were sorted regularly by FACS with either FACSAria Fusion 2 Bernhard Shoor or FACSAria Fusion 1 Richard Sweet ...
• Characterization of novel role for Rab27B in autophagy regulation in colorectal cancer

  Sahida Afroz, et al., bioRxiv - Cancer Biology 2024

  Quote: HCT116 were transfected with pBABE-puro mCherry-EGFP-LC3B plasmid (gift from Jaynta Debnath, Addgene plasmid, #22418) (reference ...
• Characterization of novel role for Rab27B in autophagy regulation in colorectal cancer

  Sahida Afroz, et al., bioRxiv - Cancer Biology 2024

  Quote: peGFP-C1 vectors containing full-length Rab27B (GFP-Rab27B, Addgene plasmid #89447) and mutant forms of Rab27B that encoded constitutively active Q78L (GFP-Rab27B Q78L ...
• Characterization of novel role for Rab27B in autophagy regulation in colorectal cancer

  Sahida Afroz, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and mutant forms of Rab27B that encoded constitutively active Q78L (GFP-Rab27B Q78L, Addgene plasmid #89448) dominant negative T23N (GFP-Rab27B T23N ...
• Characterization of novel role for Rab27B in autophagy regulation in colorectal cancer

  Sahida Afroz, et al., bioRxiv - Cancer Biology 2024

  Quote: ... dominant negative N133I (GFP-Rab27B N133I, Adddgene plasmid #89449, and geranylgeranyl-binding mutant (GER) (GFP-Rab27B GG, Addgene plasmid #89451) were gifted by Wendy Westbroek (Reference) ...
• Characterization of novel role for Rab27B in autophagy regulation in colorectal cancer

  Sahida Afroz, et al., bioRxiv - Cancer Biology 2024

  Quote: ... dominant negative T23N (GFP-Rab27B T23N, Addgene plasmid #89450), dominant negative N133I (GFP-Rab27B N133I ...
• An Innovative Mitochondrial-targeted Gene Therapy for Cancer Treatment

  Kai Chen, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and pcDNA3.1-PsChR2-eYFP (Addgene #69057) (67) ...
• Patient-derived response estimates from zero-passage organoids of luminal breast cancer

  Róża K Przanowska, et al., bioRxiv - Cancer Biology 2024

  Quote: ... using EDCPV vector (Addgene, #90085) linearized with BsmBI (NEB ...
• Patient-derived response estimates from zero-passage organoids of luminal breast cancer

  Róża K Przanowska, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Puromycin resistance (from pCW-Puro; Addgene, #50661 ...
• Patient-derived response estimates from zero-passage organoids of luminal breast cancer

  Róża K Przanowska, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and pDual_dsCas9_Venus/BFP/Puro/Blast with different sgRNA combinations or pLX302 EGFP-V5 puro (Addgene, #141348) and pLX302 TdTomato-V5 puro (Addgene ...
• Identifying and targeting key driver genes for collagen production within the 11q13/14 breast cancer amplicon

  Daniela Araiza-Olivera, et al., bioRxiv - Cancer Biology 2024

  Quote: HEK-293T cell line was transduced with a Cas9 lentiviral vector with blasticidin selection marker (Addgene# 52962) and selected with blasticidin (8 ug/ml) ...
• Identifying and targeting key driver genes for collagen production within the 11q13/14 breast cancer amplicon

  Daniela Araiza-Olivera, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and ligated into pLenti-sgRNA vector (Addgene 71409) with puromycin selection marker ...
• Opposing actions of JIP4 and RILPL1 provide antagonistic motor force to dynamically regulate membrane reformation during lysosomal tubulation/sorting driven by LRRK2

  Luis Bonet-Ponce, et al., bioRxiv - Cell Biology 2024

  Quote: ... plasmids were purchased from Addgene (Addgene#1817, Addgene#98882, Addgene#102930, Addgene#98877, Addgene#158754, Addgene#46942, Addgene#6469, Addgene#137802) (Sherer et al. ...
• Opposing actions of JIP4 and RILPL1 provide antagonistic motor force to dynamically regulate membrane reformation during lysosomal tubulation/sorting driven by LRRK2

  Luis Bonet-Ponce, et al., bioRxiv - Cell Biology 2024

  Quote: ... plasmids were purchased from Addgene (Addgene#1817, Addgene#98882, Addgene#102930, Addgene#98877, Addgene#158754, Addgene#46942, Addgene#6469, Addgene#137802) (Sherer et al. ...
• Opposing actions of JIP4 and RILPL1 provide antagonistic motor force to dynamically regulate membrane reformation during lysosomal tubulation/sorting driven by LRRK2

  Luis Bonet-Ponce, et al., bioRxiv - Cell Biology 2024

  Quote: ... plasmids were purchased from Addgene (Addgene#1817, Addgene#98882, Addgene#102930, Addgene#98877, Addgene#158754 ...
• Opposing actions of JIP4 and RILPL1 provide antagonistic motor force to dynamically regulate membrane reformation during lysosomal tubulation/sorting driven by LRRK2

  Luis Bonet-Ponce, et al., bioRxiv - Cell Biology 2024

  Quote: ... plasmids were purchased from Addgene (Addgene#1817, Addgene#98882, Addgene#102930, Addgene#98877, Addgene#158754, Addgene#46942, Addgene#6469, Addgene#137802) (Sherer et al. ...
• ERG activates a stem-like proliferation-differentiation program in prostate epithelial cells with mixed basal-luminal identity

  Weiran Feng, et al., bioRxiv - Cancer Biology 2024

  Quote: ... mNeonGreen coding sequence was synthesized and cloned into pCRISPaint-TagRFP (Addgene plasmid # 67167)128 between BamHI/NotI sites to replace the TagRFP cassette ...
• ERG activates a stem-like proliferation-differentiation program in prostate epithelial cells with mixed basal-luminal identity

  Weiran Feng, et al., bioRxiv - Cancer Biology 2024

  Quote: ... An EP organoid line with a stable transduction of lentiCas9-blast (Addgene plasmid #52962)130 was used as the starting material ...
• ERG activates a stem-like proliferation-differentiation program in prostate epithelial cells with mixed basal-luminal identity

  Weiran Feng, et al., bioRxiv - Cancer Biology 2024

  Quote: ... a targeting vector pCRISPaint-TagRFP (Addgene plasmid # 67167) was electroporated into the organoids together with CRIPSR-RNPs directed to the targeting vector (sgFrame ...
• Oncogenic Phase Transitions: How Mutant p53 Drives Amyloid Formation in p63 and p73 Liquid Droplets

  Elaine C. Petronilho, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and p73C proteins were subcloned into pET15-b (Addgene, #24866), pET28-a ...
• Oncogenic Phase Transitions: How Mutant p53 Drives Amyloid Formation in p63 and p73 Liquid Droplets

  Elaine C. Petronilho, et al., bioRxiv - Cancer Biology 2024

  Quote: ... containing a C-terminus EGFP-tagged sequence of the full-length M237I p53 protein (Addgene, #11770), and 4 µL of Lipofectamine 2000 reagent ...