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Citations for Addgene :
2201 - 2250 of 2254 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Lentiviral particles carrying a luciferase reporter were produced at the EPFL Gene Therapy Facility by transfecting HEK293 cells with the pHIV-Luc-ZsGreen plasmid (Addgene, Catalog No. 39196). Lentivirus-containing supernatants were collected and concentrated by centrifugation (1,500 g for 1 hr at 4°C) ...
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bioRxiv - Immunology 2020Quote: ... A549 cells were first made to stably express Streptococcus pyogenes Cas9 following blasticidin selection of cells transduced with lentiCas9-Blast (gift from Feng Zhang, Addgene plasmid # 52962 (19)) ...
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bioRxiv - Microbiology 2020Quote: ... were obtained by transducing wild type HCT116 colorectal carcinoma cell line (ATCC® CCL-247™) with lentiCas9-Blast lentiviral vector (Addgene #52962). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: OE19-dCas9-KRAB stable cells were generated by transfecting 1×106 OE19 cells with 7.5 μg Cas9 plasmid with guides targeting the AAVS1 locus (Addgene #42230; 5’-GGGGCCACTAGGGACAGGAT-3’) and 7.5 μg donor plasmid (pAAVS1-Puro-DNR ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293T cells were transfected with psPAX2 and pMDG.2 packaging plasmids (gifts from Didier Trono, EPFL, Lausanne, Switzerland; Addgene plasmids 12559 and 12660), the lentiviral expression plasmid ...
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bioRxiv - Biophysics 2022Quote: The plasmid encoding GPCR kinase subtype 2 used for the receptor phosphorylation in insect cells (GRK2-CAAX) was a gift from Robert Lefkowitz (Addgene plasmid #166224 49). Full-length arrestin21-418 (C150L ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293T cells were transfected with targeting plasmid and 3rd generation packing plasmid (pMD2.G, pMDLg/pRRE; Addgene #12251, and pRSV-Rev; Addgene #12253) together using Lipofectamine 2000 ...
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bioRxiv - Cell Biology 2023Quote: The pInducer20-NA7-Nef-Halo vector was packaged into lentiviruses in HEK293T cells by co- transfection with 2nd generation lentiviral packaging plasmids (Addgene 182993 and 244397) using TransIT-LT1 transfection reagent according to the manufacturer’s instructions (Mirus 2304) ...
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bioRxiv - Cancer Biology 2023Quote: HT29 dCas9-VP64 clone E cells were transduced with lentivirus of Calabrese pooled human CRISPRa library set A and B (Addgene, 92379 and 92380) separately ...
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bioRxiv - Microbiology 2022Quote: ... HEK 293T cells in 10-cm dishes were transfected at 80% confluence with the lentiviral plasmid pLenti-CMV-Puro (Addgene plasmid no. 17452) containing the gene of interest (2 mg ...
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bioRxiv - Microbiology 2023Quote: ... cells were transduced with the respective lentivirus (pWPI-IRES-Puro-Ak-ACE2, kindly provided by Dr. Sonja Best, Addgene viral prep # 154985-LV) at MOI 0.3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviral particles were packaged in HEK293T cells (ATCC: CRL-3216™) by co-transfection of pLentiCRISPR v2-β2m-gRNA with pRSV-rev (Addgene Plasmid #12253), pMDLg/pRRE (Addgene Plasmid #12251 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells expressing sgSAFE-mCherry (denoted by an additional superscript ‘C’) were generated from the respected parental cells via transduction with the lentiviral sgRNA expression vector with mCherry pMCB320 (Addgene plasmid no. 89359) virus ...
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bioRxiv - Developmental Biology 2023Quote: ... 100K-200K HEK 293 cells were reverse transfected with the M50 Super 8x TOPFlash plasmid (100 ng; Addgene #12456; gift from Randall Moon) and a construct constitutively expressing Renilla luciferase (5 ng ...
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bioRxiv - Cancer Biology 2023Quote: ... Packaging 293T cells were transfected with KEAP1 single guide RNA (sgRNA) and helper vectors (pMD2.G and psPAX2; Addgene plasmid # 12259 and 12260) using Lipofectamine 3000 reagent (Cat ...
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bioRxiv - Genetics 2023Quote: Lentiviruses were produced by co-transfection of HEK293T cells with the relevant lentiviral transfer vector and packaging vectors psPAX2 (Addgene, catalog no. 12260) and pMD2.G (Addgene ...
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bioRxiv - Genetics 2024Quote: ... Lentivirus was produced from sgRNA plasmids by co-transfecting Lenti-X 293T (Takarabio, 632180) cells with the sgRNA plasmid library and packaging plasmids-psPAX2 and pMD2.G (Addgene, 12260 and 12259) using Trans-IT-293 transfection reagent (Mirus ...
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bioRxiv - Immunology 2024Quote: Compound knockouts of B16 cells were generated by sequential lentivirus transduction using pLenticrispr v2 (gift from Dr. Feng Zhang, Addgene #52961, puromycin selection), lenti-sgRNA blast (gift from Dr ...
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bioRxiv - Biochemistry 2024Quote: HEK293 knockout cell lines were generated using the pSpCas9(BB)-2A-GFP (PX458) CRISPR/Cas9 construct (a gift from F. Zhang; Addgene, plasmid 4813; 55) as described previously 56 ...
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bioRxiv - Cancer Biology 2024Quote: ... 70-80% confluent cells were transfected with pMRX-IPU-FLAG-NEK9 or pMRX-IPU-FLAG-NEK9T210A along with pCL-Eco plasmid (Addgene, Cat No# 12371) that was graciously donated by Inder Verma (Naviaux et al ...
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bioRxiv - Microbiology 2022Quote: ... a total of 300 million A549-Cas9 cells were then transduced with the lentiviral human GeCKO v2 library (Addgene #1000000049, gift from Feng Zhang)(29 ...
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bioRxiv - Molecular Biology 2021Quote: ... Lentivirus was produced in HEK293T cells using a 2nd generation lentiviral packaging system (psPAX2 – Addgene plasmid #12260 and pMD2.G – Addgene #12259 from Didier Trono) and pRRL.EF1a.HA.NLS.Sce(opt).T2A.BFP (Addgene #32628 from Andrew Scharenberg ...
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bioRxiv - Genetics 2020Quote: ... OCI-AML3 and K562 cells were first transduced with the lentiviral vector pHR-SFFV-KRAB-dCas9-P2A-mCherry (Addgene 60954, deposited by Jonathan Weissman), sorted for mCherry on a FACSAria II ...
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bioRxiv - Cell Biology 2021Quote: ... or hTMIGD1 cDNAs were generated by co-transfection of HEK293T cells with the lentiviral vector and the packaging vectors psPAX2 and pMD2.G (kindly provided by Dr Didier Trono, Addgene plasmids 12260 and 12259) in a ratio of 3:2:1 into HEK293T cells ...
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bioRxiv - Microbiology 2020Quote: Lentivirus-derived VLPs were produced by transfecting 10 cm dishes of HEK293T LentiX cells with lentiviral packaging plasmids encoding Gag/Pol (pMDLg/pRRE, 15 µg) and Rev (pRSV-REV, 6 µg) (Addgene plasmids 12251 and 12253) together with vectors expressing SARS-CoV-2 Spike (13 µg ...
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bioRxiv - Microbiology 2020Quote: ... a total of 240 million Huh7.5.1-Cas9-blast or Huh7.5.1-Cas9-blast+ACE2-IRES-TMPRSS2-hygro cells were transduced with lentivirus of the human GeCKO v2 library (Addgene, #1000000049, gift from Feng Zhang) at a moi of 0.4 and subsequently selected using puromycin and expanded for 7 days ...
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bioRxiv - Cancer Biology 2020Quote: WM266-4 or SK-MEL-2 cells were co-transduced with conditioned media containing lentiviruses bearing Firefly luciferase-7TFP (Addgene #24308, β-catenin reporter) and Renilla luciferase pLenti.PGK.blast-Renilla Luciferase (Addgene #74444 ...
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bioRxiv - Molecular Biology 2022Quote: ... baculovirus gp64 signal sequence under control of the very late polyhedrin promoter was inserted into the insect cell vector plasmid pACEBac1 (Addgene, LGC Standards Teddington, UK) by using another synthetic gene (VK18 ...
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bioRxiv - Molecular Biology 2020Quote: ... In the same cell line a functional cGAS was reconstituted by transducing either a lentiviral cGAS-RFP or cGAS-GFP (Addgene plasmids # 86676 and # 86675), a gift from Nicolas Manel (30) ...
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bioRxiv - Biophysics 2020Quote: ... Lentiviruses were produced in 293T cells by transfecting lentiviruses with the helper plasmids pMD2.G and psPAX2 (a gift from D. Trono, Addgene plasmids #12259 and #12260), following Addgene’s instructions ...
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bioRxiv - Genetics 2020Quote: Barcoded MSH2-2A-blR cDNA libraries were packaged into lentivirus by co-transfecting HEK293T/17 cells (ATCC) with the transfer plasmid pool plus envelope and packaging vectors (pMD2.G, Addgene #12259 and psPAX2, #12260). For each pool ...
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bioRxiv - Cell Biology 2021Quote: ... and EGFP-α-SynA53T plasmid for α-Syn expression in HEK293T and SH-SY5Y cells was a gift from David Rubinsztein (Addgene plasmid #4082385)).
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bioRxiv - Microbiology 2020Quote: Lentiviruses derived from pLKO or pGIPZ were derived from transfection of 293T cells with packaging plasmids pMD2.G and psPAX2 (gifts from Didier Trono Addgene plasmids 12259 and 12260) using polyethylenimine MAX (polysciences #24765) ...
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bioRxiv - Molecular Biology 2022Quote: ... Lentiviral particles were then prepared by cotransfection of pLentiCRISPRv2 constructs into HEK293T cells with pCMV-VSV-G (Addgene #8454, a gift from Bob Weinberg) and psPAX2 (Addgene #12260 ...
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bioRxiv - Cancer Biology 2022Quote: ... Viral supernatants were prepared by transfection of HEK293T cells with the vector plasmids and packaging plasmids (psPAX2 Addgene 12259 and pMD2.G Addgene 12260). The transfection was performed with polyethylenimine (PEI MAX Transfection Grade Linear Polyethylenimine Hydrochloride ...
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bioRxiv - Microbiology 2022Quote: ... Dimeric human ACE2-IgG1 (hACE2) was generated by transfecting Expi293 cells with pcDNA3-sACE2-WT(732)-IgG129 (Addgene plasmid #154104, gift from Erik Procko) using 1 mg mL-1 PEI and then purifying from the supernatant five days post-transfection using rProtein A Sepharose (GE ...
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bioRxiv - Cell Biology 2023Quote: Lentiviruses were generated in HEK293T cells by transient expression of the indicated shRNA vectors below with psPAX2 and pMD2.G packaging vectors (Addgene plasmids 11260 and 12259). For lentiviral production in 96 well plates ...
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bioRxiv - Cancer Biology 2023Quote: Lentivirus was produced in 293T cells by co-transfecting NF-κB reporter (41) with packaging vectors psPAX2 (Addgene #12260, a gift from Didier Trono) and pMD2.G (Addgene #12259 ...
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bioRxiv - Cell Biology 2023Quote: ... Lentiviruses were generated in HEK293T cells by transient expression of the vectors with pSPAX2 and pMD2.G packaging vectors (Addgene plasmids #12260 and #12259). Viral supernatants were collected after 48 h of expression and passed through a 45 μm syringe filter before exposure to target cells.
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviruses were generated by transfecting sub-confluent HEK293T cells along with the lentiviral vector and packaging vectors pCMV-VSV-G (Addgene, Cat. 8454, RRID: Addgene_8454) and psPAX2 (Addgene ...
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bioRxiv - Microbiology 2024Quote: ... The plasmid library was sequenced to confirm representation and packaged into lentivirus in HEK293T cells using plasmids psPAX2 (Addgene #12260, gift from Didier Trono) and pCMV-VSV-G (Addgene #8454 ...
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bioRxiv - Cancer Biology 2024Quote: Lentiviruses were produced by transfecting 293FT cells with the selected vector and essential packaging plasmids (pSPAX2 and pMD2.G, gifts from Didier Trono; Addgene plasmid 12260 and 12259). Post-transfection ...
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bioRxiv - Genetics 2020Quote: ... Ishikawa cells were plated at a density of ~300,000 cells per well in 6 well plates and transfected the following day with 1650 ng Cas9 plasmid (Addgene 62988, a gift from Feng Zhang), 550 ng of each guide RNA (Table S2) ...
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bioRxiv - Microbiology 2020Quote: ... a total of 300 million Huh7.5.1-Cas9 cells were then separately transduced with the lentiviral gRNA sublibraries A and B of the human GeCKO v2 library (Addgene #1000000049, gift from Feng Zhang) at a multiplicity of infection (MOI ...
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bioRxiv - Cell Biology 2022Quote: 6.0 × 10^6 BJAB clone B2 cells expressing both Cas9 and ERAAP dsRed were transduced with lentivirus of human GeCKO v2 library (Addgene, #1000000049, gift from Feng Zhang) at an MOI of 0.4 using spin-infection (1,000 g for 2 h at 33 °C) ...
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bioRxiv - Systems Biology 2022Quote: ... a total of 240 million A549-Cas9 cells were transduced with the lentivirus of the human GeCKO v2 library (Addgene #1000000049, gift from Feng Zhang) (48 ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were transiently transfected with green fluorescent protein (eGFP) (0.25 µg of cDNA/ml) and PIEZO1 channel plasmid (Addgene, #80925, 3 µg of cDNA/ml). For control experiments ...
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bioRxiv - Cell Biology 2022Quote: ... IFT121 and IFT139 in IMCD-3 cells were designed using Benchling software and cloned into the pX330 vector (gift from Feng Zhang; Addgene plasmid # 42230; (Cong et al., 2013)) ...
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bioRxiv - Microbiology 2022Quote: All lentiviruses vector particles were generated in HEK-293T cells by co-transfection with plasmids pMD2.g and psPAX2 (Addgene # 12259, #12260, gift of Didier Trono), exactly as described previously (43) ...
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bioRxiv - Cell Biology 2024Quote: To generate RRBP1 KO HEK293T cells expressing EGFP-FLAG-APEX2-ePTS1 single gRNA transcriptional cassette prepared by PCR and pCAS9-mCherry empty (Addgene, 80975 (Schmid- Burgk et al., 2016)) were co-transfected into HEK293T cells expressing EGFP-FLAG-APEX2- ePTS1 ...