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Citations for Addgene :
2151 - 2200 of 2254 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... The Lenti-iCas9-Neo vector containing the Flag-iCas9-P2A-GFP cassette for Cas9 expression in K562 cells was obtained as a gift from Qin Yan (Addgene plasmid #85400,35). For Cas9 expression in HEK293T cells ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we first replaced the SH3 domain with a flexible linker (GGSSGGGG) using yeast competent cells that were co-transformed with a pCAS plasmid (Addgene plasmid 60847) expressing both the gRNA of interest and Streptococcus pyogenes Cas9 and a donor DNA sequence (stuffer ...
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bioRxiv - Cancer Biology 2023Quote: Lentivirus containing all mentioned plasmids was generated by transfecting HEK293T cells with the appropriate plasmid along with the psPAX2 (Addgene; Plasmid #12260) and pMD2.G (Addgene ...
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bioRxiv - Neuroscience 2023Quote: ... were cloned into either a Lentiviral CMV-driven construct for use in cell culture experiments (shown in Figure S2) or a GFAP-GFP Adeno-associated virus (AAV) construct (Addgene plasmid #50473) for use in animal experiments (utilized in Figure 3 and Figure 4) ...
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bioRxiv - Immunology 2022Quote: ... Virus was generated by transfecting the sgRNA plasmid constructs into HEK 293T cells with the psPAX2 packaging (Didier Trono, Addgene plasmid #12260) and pCMV-VSVG envelope (Bob Weinberg ...
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bioRxiv - Cell Biology 2023Quote: ... pDsRed-RAB11A WT (for expression of a fluorescently-tagged, wild-type version of RAB11A in human cells) was obtained from Addgene (plasmid 12679). For expression of GFP-HEATR5B from baculovirus ...
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The Hippo pathway terminal effector TAZ/WWTR1 mediates oxaliplatin sensitivity in colon cancer cellsbioRxiv - Cancer Biology 2023Quote: Murine Taz was expressed by transfecting cells with pEF-TAZ-N-Flag from Michael Yaffe (Addgene #19025; RRID:Addgene_19025; Kanai et al., 2000).
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bioRxiv - Genetics 2023Quote: A phenotypically WT (ROSA+/cre CTIPc/c Bcl2+) v-abl immortalized B cell line was infected with lentivirus for doxycycline-inducible SpCas9 (Addgene Plasmid #50661). Single clones were generated and screened for high SpCas9 inducibility after doxycycline treatment (Sigma Aldrich ...
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bioRxiv - Systems Biology 2023Quote: ... HeLa Cells were transduced with the lentiviral expression vector pLenti-H2B-iRFP720 (a gift from Carlos Carmona-Fontaine (Addgene plasmid # 128961; RRID:Addgene_128961) to express fluorescently labeled H2B proteins according to methodology described previously (Segeren et al ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa or HEK293FT cells were co-transfected with the sgRNA plasmid and the pST1374-NLS-flag-linker-Cas9 (Addgene 44758, Xingxu Huang). After 24 h ...
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bioRxiv - Cell Biology 2023Quote: Lentiviral expression constructs were packaged in HEK293FT cells using calcium phosphate transfection of psPAX2 and pMD2.G (kind gifts of D. Trono; Addgene #12260, #12259) and pTAT (kind gift of P ...
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bioRxiv - Genetics 2023Quote: ... EPP2 and RN2 cells were retrovirally transduced with shRNAmir constructs cloned into pMSCV-miR-E-PGK-Neo-IRES-mCherry backbone (LENC; Addgene plasmid #111163), and initial infection levels were determined by flow cytometry based on mCherry expression 4 days post transduction (day 0).
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bioRxiv - Genomics 2023Quote: Plasmids in the pCAG backbone used to overexpress TWIST1 and ALX4 in HEK293 cells were cloned by digesting the pCAG-NLS-HA-Bxb1 plasmid (Addgene plasmid # 51271) prepared from dam-/dcm- E ...
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bioRxiv - Biochemistry 2022Quote: ... The following plasmids used for DNA damage experiments in HeLa Tet-On cells were gifts from Kathleen Burns33: pDA007 (Addgene plasmid # 131380), pDA025 (Addgene plasmid # 131384) ...
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bioRxiv - Biochemistry 2023Quote: ... UT Health Sciences Center at San Antonio) and insect cell codon-optimized full-length BARD1 (pFastBac-Twin-StrepTagII-BARD11-777; gift from A. Deans, Addgene plasmid #137166)(Tan et al. ...
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bioRxiv - Developmental Biology 2023Quote: To measure mitophagy in HUVEC were seeded in 6-well plates (8 x 105 cells/well) and transfected with 5ug/well of pCHAC-mt-mkeima plasmids (Addgene plasmids #72342) at a ratio of 1:1.5 plasmids ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-MARCO-CHO and GFP-MARCO-HEK cells were further transduced with RFP-LC3 plasmid which was a gift from Tamotsu Yoshimori (Addgene, plasmid #21075) (Kimura et al ...
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bioRxiv - Systems Biology 2023Quote: ... ura3 his3 met15 leu2::GEM gal4::NAT gal1::stuffer-linker-GFP-HIS3) competent cells were co-transformed with a pCAS plasmid (Addgene plasmid 6084747) expressing both the gRNA for the stuffer (Dionne et al 2021 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were expanded and transfected (day 0) with PEI at a coverage of > 500x with pCMV-PE2-tagRFP-BleoR (Addgene no. 192508). One day later ...
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bioRxiv - Cell Biology 2023Quote: Stable MEL cell clones expressing dCAS9-KRAB were generated using Lenti-dCAS9-KRAB-blast (Gift from Dr. Gary Hon, Addgene plasmid #89567). 2 × 106 of MEL cells were suspended in 100ul of High-Performance Electroporation Solution (BTXpress ...
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bioRxiv - Developmental Biology 2023Quote: HEK293T cells were plated at a density of 2.8×105 cells in 6-well plates and transfected with MSCV-flag-PRDM16 (Addgene, 15504; RRID:MSCV PRDM16) and/or pCDNA3-NKX2-174 using Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 (DE3) cells using the plasmids pRSFDuet-1 His6-SUMO-CoiA1_CoiB and pETDuet-1 Coi_CoiSA(ED) (Addgene ID #208761 and #208762), following the method previously reported.10 For the preparation of methyllanthionine standard ...
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bioRxiv - Cell Biology 2023Quote: Donor plasmid encoding homology arms and linker-mEGFP sequence for C-terminus tagging of human NPM1 was designed by the Allen Institute for Cell Science and obtained from Addgene (AICSDP-50). The plasmid encoding NPM1-mEGFP was a gift from the Allen Institute for Cell Science (Addgene plasmid # 109122) ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293T cells were triple transfected with lentivirus packaging plasmid psPAX2 and the envelope plasmid pCMV-VSV-G (Addgene plasmids #12260 and #8454) in addition to the transgene of interest using FuGene HD (Promega) ...
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bioRxiv - Biophysics 2024Quote: ... the cells were co-transfected with two plasmids: pAS_4xU6-PyIT M15(UUA) FLAG-Mma PyIRS (Mma PylT/RS) from Addgene (Watertown, MA, #154774)25 and the Acd RS1 tRNA/aminoacyl-tRNA synthetase pair (GCE4All Research Center ...
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bioRxiv - Microbiology 2024Quote: ... Oligos encoding sgRNAs for generating knockout cells using CRISPR–Cas9 were cloned into the lentiCRISPRv2 plasmid (a gift from F. Zhang, Addgene plasmid 52961) as previously described [66] ...
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bioRxiv - Cell Biology 2024Quote: ... the CAAX domain of K-ras tagged with mTagRFP-T was introduced into a safe harbor locus via CRISPR using a donor plasmid developed at the Allen Institute for Cell Science and sourced from Addgene (Plasmid #107580a). We call the resulting cell line CT20 ...
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bioRxiv - Cell Biology 2024Quote: ... MDA-MB-231 cells were transfected with the engineered pSBtet-BP::eGFP-PLCδ-PH and pCMV(CAT)T7-SB100 plasmid (Addgene, Plasmid #34879) using FuGENE HD transfection reagent (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... The ppyCAG_RNaseH1_WT employed for the transient overexpression of RNAseH1 in vitro on HEPA1-6 cells was a gift from Xiang-Dong Fu (Addgene plasmid #111906).
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bioRxiv - Neuroscience 2024Quote: ... Cell-type specific expression of Gcamp6s and channelrhodopsin was achieved using the following Cre-dependent viruses: AAV5-FLEX-GcAMP6s (Addgene #100842-AAV5) for ROb/Pa and RMg ...
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bioRxiv - Molecular Biology 2024Quote: We generated HeLa cells with an integrated GFP locus (HeLa-GFP) by transducing cells with the pLenti-CMV-MCS-GFP-SV-puro lentiviral plasmid (Addgene Plasmid #73582). Cells were cultured in DMEM (Euroclone ...
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bioRxiv - Molecular Biology 2024Quote: ... 501mel cells were transfected with DDX50 or DUSP12 HA-targeting plasmids together with the pSpCas9(BB)-2A-Puro vector (Addgene plasmid #62988) containing a gRNA sequence targeting each of the proteins ...
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bioRxiv - Cell Biology 2024Quote: ... Lentivirus was produced by transient transfection of pCW57-TOM40-APOE3 or T9A2-APOE4 into 293T cells along with the envelope plasmids pMD2.G (Addgene Cat. 12259) and psPAX2 (Addgene Cat ...
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bioRxiv - Bioengineering 2024Quote: HeLa cells expressing mCherry-coupled galectin-9 (mCherry-GAL9) were obtained by transfection using plasmids encoding mCherry-galectin 9 (Addgene, Watertown, MA). The galectin-9 reporter HeLa cells were then cultured in ibidi μ-slide 8 well glass bottom chamber at a density of 2x104 cells/well and grew overnight ...
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bioRxiv - Molecular Biology 2024Quote: Lentiviral expression vectors were co-transfected into 293FT cells with the lentiviral packaging plasmids psPAX2 and pMD2.G (Addgene #12260 and #12259). Briefly ...
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bioRxiv - Immunology 2024Quote: Lentiviral particles containing the miBds libraries were produced by lipofectamine-based co-transfection of HEK293 cells with 3rd generation packaging plasmids pMD2.G (Addgene, Cat.#12259), pMDLg/pRRE (Addgene ...
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bioRxiv - Bioengineering 2024Quote: ... 8 × 106 reporter cells were transduced in 6-well format with 1 ml Human Brunello CRISPR knockout pooled library (Addgene #73178 31) in the presence of 10 μg/ml polybrene (Merck Millipore) ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmid vector pLC-Flag-CRBN-P2A-Hygro for generation of stable BE2C cell line overexpressing CRBN was a gift from Eva Gottwein (Addgene plasmid #124303). HEK293 cells with stable gene overexpression were generated by lentiviral-mediated infection of cells according to published protocols62 ...
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bioRxiv - Cell Biology 2024Quote: Second generation lentiviral particles were prepared by co-transfecting HEK-293T cells with the plasmid of interest (pMD2.G (Addgene, cat. #12259), psPAX2 (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: 621-101 and TSC2 addback cells were infected by lentiviral transduction with dual-color fluorescent reporter for CD63-positive exosome secretion and uptake (Addgene plasmid # 172118), as previously described9 ...
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bioRxiv - Cancer Biology 2024Quote: ... Non-replicating lentiviruses were generated by transient co-transfection of the transfer plasmids into HEK293T cells together with the packaging plasmids pMDL (Addgene plasmid #12251), pRSV-REV (Addgene plasmid #12253) ...
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bioRxiv - Developmental Biology 2024Quote: Retroviruses carrying the full-length coding sequence of Prdm16 or mutant Prdm16 were also produced by co-transfecting HEK 293T cells with MSCV PRDM16 expression vector (Addgene, plasmid #15504), pBS-CMV-gag pol (Addgene ...
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bioRxiv - Developmental Biology 2024Quote: ... The donor plasmid contained a pCAGG::mCherry integration reporter for non-antibiotic selection which could be removed by CRISPR/Cas9 using flanking tia1L guide sites (Roberts et al., 2019, fragments taken from AICSDP-45: TTN-mEGFP, a gift from Allen Institute for Cell Science; Addgene # 114412). A homology region of ∼2kb in length surrounding the FOXN4 stop codon was amplified using PCR for HDR targeting (Supplemental Table 4) ...
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bioRxiv - Biochemistry 2022Quote: ... These GFP positive cells were transduced with lentiviral particles for pLenti CMV rtTA3 Hygro (w785-1) (a gift from Eric Campeau Addgene plasmid number 26730) for tetracycline inducible expression and selected using hygromycin (200μg/ml) ...
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bioRxiv - Developmental Biology 2021Quote: ... AAVS1 repair template was created by Infusion cloning to swap the CAG promoter and Puromycin resistance cassette in plasmid AICSDP-42: AAVS1-mTagRFPT-CAAX (a gift from The Allen Institute for Cell Science, Addgene plasmid # 107580). SOX2 knockout repair template was created by Infusion cloning to insert SOX2 5’ and 3’ homologous arms in EasyFusion T2A-H2B-GFP (a gift from Janet Rossant ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lines stably expressing the Cas9 nuclease were generated by infection with the empty lentiCRISPRv2 lentivirus (Addgene, #52961, not containing an sgRNA guide) using standard methods (https://www.addgene.org/viral-vectors/lentivirus/lenti-guide/) ...
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bioRxiv - Cancer Biology 2020Quote: pBABE-SAOS 2 and hTERT-SAOS 2 stable cell lines were obtained upon transfection of the SAOS 2 cell line with the plasmids pBABE-puro or pBABE-puro-hTERT from Addgene (#1764, #1771, respectively), and Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: Stably Cas9-expressing HepG2 cells were transduced with genome-wide lentiviral pooled CRISPR/Cas9 knockout library (Addgene #73178) (Brunello; Cat# 73178-LV) (Addgene, Inc., Watertown, MA) containing 76,441 sgRNAs ...
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bioRxiv - Molecular Biology 2022Quote: ... Conditional knockout cells were generated by infection with media from a confluent 10 cm dish of 293T cells transfected with 3 µg pCL-Eco (Addgene, 12371 ref (17)) and 3 µg MSCV CreERT2 puro (Addgene ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293T cells were transfected with psPAX2 and pMDG.2 packaging plasmids (gifts from Didier Trono, EPFL, Lausanne, Switzerland; Addgene plasmids 12559 and 12660) and the lentiviral expression construct ...