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Citations for Addgene :
2101 - 2150 of 2254 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: ... The lentiCRISPRv2 vectors (30 µg) containing respective guide RNAs were transfected to the cells together with 20 µg of psPAX2 (Addgene, Cat#8454) and 10 µg of pCMV-VSV-G (Addgene ...
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bioRxiv - Molecular Biology 2021Quote: ... was randomly integrated in the genome of the target cell line via lentiviral transduction of a modified version of pHR-SFFV-dCas9-BFP-KRAB (Addgene plasmid # 46911) carrying a P2A blasticidin selection and UCOE element (kind gift of Marvin Tanenbaum) ...
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bioRxiv - Cancer Biology 2020Quote: To generate lentiviral particles for SMPD3-P2A-Luc2 GFP and Luc2 GFP the lentiviral vector was packaged in LentiX HEK293T cells using the packaging plasmids psPAX2 (Addgene Plasmid #12260) and pMD2.G (Addgene Plasmid #12259) ...
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bioRxiv - Cancer Biology 2020Quote: For this assay cells were co-transfected with M50 Super 8x TOPFlash (which was a kind gift from Randall Moon, Addgene, Cat #12456) 12 and pRL-TK (Promega ...
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bioRxiv - Systems Biology 2021Quote: Growing REF/E23 cells were kept at sub-confluence and transfected with pfmh-hCry1 (a gift from Aziz Sancar; Addgene plasmid #25843) and pAdTrack-CMV FLAG Rev-erbα expression vectors (a gift from Bert Vogelstein ...
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bioRxiv - Cell Biology 2021Quote: ... or ‘CRISPR-control’ (vector with no insert) HeLa cell lines were generated by lentiviral transduction using the lentiCRISPRv2 plasmid (a gift from Feng Zhang - Addgene plasmid #52961). Cells were selected with 3µg/mL puromycin until complete uninfected cell death occurred ...
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bioRxiv - Cancer Biology 2020Quote: ... NCI-H3122 and EKVX cells were stably transfected with pKrasG12C or its homologous GFP backbone plasmid without KrasG12C (pC; Addgene #64336; Bicistronic_GFP_ires_puro) using previously established methods (Agalioti et al. ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... SH-SY5Y cells stably expressing YFP-alpha-synuclein were obtained by lentiviral transfection using 3rd generation lentiviruses (Addgene constructs: 12251, 12253, 12259) (67) ...
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bioRxiv - Neuroscience 2020Quote: ... Viral vector stocks were produced by calcium phosphate transfection of 293FT cells in combination with packaging vectors psPAX2 (a generous gift from Didier Trono; Addgene plasmid # 12260) for lentiviral constructs ...
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bioRxiv - Biochemistry 2021Quote: Lentiviral expression constructs were packaged in HEK293FT cells using calcium phosphate transfection of psPAX2 and pMD2.G (kind gifts of D. Trono; Addgene #12260, 12259) and pTAT (kind gift of P ...
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bioRxiv - Microbiology 2021Quote: ... Infectious viral particles with each of these lentiviral constructs were produced by co-transfecting 293T cells with packaging plasmid psPAX2 (Addgene, Cat#12260) and Env Vector pCMV-VSVg (Addgene ...
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bioRxiv - Cell Biology 2021Quote: ... A control cell line was generated by infecting U2OS cells stably expressing a non-targeting sgRNA (above) with the lentivirus vector pLKO.1-blast-Scramble (Addgene, cat# 26701) expressing a non-targeting shRNA sequence and selected with 15µg/ml blasticidin for 7 days ...
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bioRxiv - Cell Biology 2020Quote: ... CRISPR-edited HaCaT BACH1-KO and HaCaT NRF2-KO/BACH1-KO cells were generated by transfecting either HaCaT WT or HaCaT NRF2-KO cells with two different pLentiCRISPR-v2 (a gift from Dr Feng Zhang, Addgene plasmid #52961) containing each one a guide RNA against the first exon and the second exon of BACH1 ...
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bioRxiv - Cell Biology 2021Quote: ... Raji or SKW6.4) were infected with Cas9 expressing viruses that were produced in HEK293T cells transfected with the lentiCas9-Blast (#849, Addgene plasmid #52962), pMD2.G ...
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bioRxiv - Cell Biology 2021Quote: ... BY4741 cells and mutant strains from the BY4741 gene knockout library were made prototrophic by transformation with the pHLUM plasmid (Addgene, Watertown, Massachusetts) [40] ...
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bioRxiv - Cancer Biology 2021Quote: ... or RUNX1ΔEx6 amplified from cDNA obtained from 293T cells and the pINDUCER-21-RUNX1 plasmid (Addgene plasmid #97043; http://n2t.net/addgene:97043; RRID:Addgene_97043 (Sugimura et al., 2017)) ...
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bioRxiv - Biochemistry 2022Quote: ... VSV-G-pseudotyped lentivirus was generated by cotransfection of HEK-293T cells (CRL-3216, ATCC, Manassas, VA) with psPAX2 (gift from Didier Trono, Addgene plasmid #12260), pMD2.G (gift from Didier Trono ...
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bioRxiv - Biophysics 2022Quote: ... media was refreshed with standard growth media and cells were co-transfected with SNAP-mGluR2 (no FLAG-tag) and chimeric G protein (Gqo5, Addgene plasmid #24500) (1:2 w/w ...
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bioRxiv - Cancer Biology 2022Quote: MCF7 cells engineered to express dCAS9-KRAB (details in main methods) and parental MCF7 cells were infected with the pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W plasmid (Addgene plasmid # 67980). For the infection the virus was generated in 293FT cells transfected with PAX2 and pMD2.G plasmids together with the pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W plasmid using the X-tremeGENE reagent (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... INS-1 832/3 EV or g1-2 SNAP-GLP-1R cells were transfected with the pCI HA NEDD4 construct (a gift from Prof. Joan Massague, Addgene plasmid #27002) 24 hours before the experiment ...
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bioRxiv - Molecular Biology 2020Quote: ... EJ5-GFP HCT116 cells and EJ5-GFP U2OS reporter cells were similarly generated by selecting stable clones after electroporation of HCT116 and U2OS cells with XhoI linearized pimEJ5-GFP (25) (Addgene, plasmid 44026) DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... Expression constructs needed to generate biotinylated anti-GFP nanobody for native purification from human cells are available from Addgene (#149336, #149334, #149333) (Pleiner et al. ...
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bioRxiv - Microbiology 2021Quote: ... FMNL3-V5 was expressed in U937 FMNL3 KO using lentiviruses generated in 293E cells co-transfected with the packaging constructs psPAX2 (Addgene plasmid 12260) and pCMV-VSV-G (Addgene plasmid 8454).
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bioRxiv - Cancer Biology 2020Quote: ... To knock-out Psat1 in cells isolated from MYC-driven tumours induced in Psat1fl/fl mice the retroviral vector MSCV-CreERT2 puro (Addgene plasmid #22776) was used ...
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bioRxiv - Cancer Biology 2020Quote: ... The resulting HeLa-dCas9 cells were then transduced with the lentiviral construct for the MS2-P65-HSF1 (MPH) activator complex (Addgene 61426-LVC) and selected with 0.5 mg/ml hygromycin ...
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bioRxiv - Microbiology 2021Quote: Cells stably expressing shRNA of different genes under the control of a tetracycline-inducible promoter were created by first transducing SNB-19 cells with lentivirus particles containing pLenti CMV TetR BLAST (Addgene; number 17492). The cells underwent selection with media containing 10 μg/mL of blasticidin (Invivogen ...
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bioRxiv - Genomics 2021Quote: ... Lentivirus production was carried out by co-transfecting HEK293T cells with 12.5 μg of Cas9 plasmid with blasticidin resistance (Addgene, cat. no. 52962), 7.5 μg psPAX2 plasmid and 4 μg the packaging pVsVg plasmids ...
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bioRxiv - Cell Biology 2020Quote: ... HeLaH2B-GFP- and HeLa2FPs-KO-PA28γ cell lines were generated by cotransfection of PSME3/PA28γ sgGuide and pX459 vectors (a gift from Feng Zhang, Addgene plasmid #62988), and cells were selected with puromycin (1 μg/ml) ...
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bioRxiv - Cell Biology 2021Quote: HEK-293T cells plated on polylysine-coated coverslips were transfected with cDNA (100 ng/40000 cells) coding for mitochondrial-targeted mCherry (mCherry-Mito-7, a gift from Michael Davidson (Addgene plasmid # 55102), (Olenych et al ...
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bioRxiv - Immunology 2020Quote: ... Lentiviruses were generated by co-transfecting Lenti-X 293T cells with the lenti-vector and the packaging plasmids psPAX2 (Addgene Plasmid #12260) and pMD2.G (Addgene Plasmid #12259 ...
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bioRxiv - Immunology 2021Quote: ... DFT cells were co-transfected with pCO2 and SB transposase vector pCMV(CAT)T7-SB10025 (a gift from Zsuzsanna Izsvak; Addgene plasmid # 34879) at a ratio of 3:1 in μg ...
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bioRxiv - Microbiology 2021Quote: ... Lentivirus was produced by triple transfection of 293T cells with the sgRNA expression LentiCRISPR-v2 vector and the packaging plasmids psPAX2 (Addgene plasmid # 12260) and pMD2.G (Addgene plasmid # 12259 ...
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bioRxiv - Molecular Biology 2020Quote: ... The TRIPZ plasmid was transfected into 293T cells along with lentiviral packaging and envelope 2nd generation plasmids (Addgene packaging 11263, Addgene envelope 17576). 48 hours post-transfection ...
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bioRxiv - Developmental Biology 2020Quote: ... Rcho1 cells were stably cotransfected with the vectors (phU6-gRNA) expressing enhancer gRNAs and Cas9 (pLV hUbc-Cas9-T2A-GFP, Addgene, Plasmid #53190)[32] using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... this cell line was co-transfected with a 9:1 ratio of pCENP-B-Cherry (a gift from Michael Lampson; Addgene plasmid #45219) and pPGKpuro resistance plasmid ...
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bioRxiv - Genomics 2021Quote: All gRNAs used for HEK293T cells and mESCs targeting have been cloned into the double BbsI sites of pX330-vector (Addgene ID 42230). The plasmid used for CH12F3 cell targeting was an optimized vector in which we removed the AAV2 ITR sequence and introduced a mCherry gene with CMV promoter by Gibson assembly into pX330 vector.
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bioRxiv - Genetics 2020Quote: ... this cell line was transfected with a plasmid expressing Bxb1 integrase (pCAG–NLS–HA–Bxb1; Addgene #51271, a gift from Pawel Pelczar36) by transient transfection with FuGENE 6 (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: ... CRISPR-mediated gene disruption was performed by identifying individual gRNA sequences with consistent enrichment in LDLlow cells in our previously reported CRISPR screens [7] and cloning these sequences into the BsmBI sites of pLentiCRISPRv2 (Addgene #52961, [43]) or into the BbsI sites of pX459 (Addgene #62988 ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentivirus was generated using 293FT cells by co-transfection of guide plasmid with psPAX2 and pMD2.G (Addgene, 12260 and 12259, respectively). Conditioned media was harvested ...
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bioRxiv - Cell Biology 2022Quote: ... K562 CRISPRi cells with or without MICU1(GFP1-10) were nucleofected with a MTCH2 targeting guide in the pX458 backbone (Addgene plasmid # 48138) using the Lonza SF Cell Line 96-well Nucleofector Kit (V4SC-2096) ...
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bioRxiv - Genomics 2022Quote: Lentiviral particles were produced by transfecting 293T-17 cells (ATCC: CRL-11268) with the envelope construct pCMV-VSV-G (Addgene plasmid 8454), the packaging construct pCMV-dR8.2 dvpr (Addgene plasmid 8455) ...
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bioRxiv - Immunology 2022Quote: ... the cells were transduced with virus produced using the lentiCas9-EGFP plasmid (a gift from Phil Sharp & Feng Zhang, Addgene plasmid # 63592), single-cell sorted using a Sony SH800 cell sorter ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa cells were generated by infecting cells with lentivirus containing a Cas9-P2A-GFP expression cassette under a doxycycline inducible promoter as described previously (Addgene plasmid #85400)50 ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli chromosome at the λ integration site by electroporating this plasmid into DH10B cells containing the helper plasmid pInt-ts (Addgene #66076) and selecting for kanamycin resistant colonies ...
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bioRxiv - Immunology 2024Quote: ... Production of lentiviral particles was performed by transient co-transfection (polyethylenimine:DNA at 3.5:1 ratio) of HEK 293T cells with the second-generation packaging system plasmid psPAX2 and pMD2.G (Addgene, 12260 and 12259). Viral supernatants were harvested at 48h post-transfection ...
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bioRxiv - Molecular Biology 2024Quote: Lentivirus particles for transduction of IMR90 E6E7 hTERT cells were produced by transfection of an expression plasmid with packaging and envelope plasmids gifted from Didier Trono (Addgene plasmid #12260) and Robert A ...
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bioRxiv - Cancer Biology 2023Quote: ... and SNAI1 knockdown cells were generated by cloning respective shRNA sequences into the 3rd generation lentiviral plasmid pLKO.1 TRC cloning vector (Addgene, cat# 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter ...
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bioRxiv - Biochemistry 2024Quote: ... Lentiviral particles were produced in HEK293 cells after transient transfection of the packaging vectors psPAX.2 and pMD.G2 (Addgene #12260 and #12259). After viral transduction ...
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bioRxiv - Cancer Biology 2024Quote: To generate ER-Hoxa9 mCherry-Geminin cells, the mCherry-Geminin fusion gene (Sakaue-Sawano et al., 2008) was cloned into the pLV-Hygro vector (Addgene plasmid #85134), using Gibson cloning ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were transfected with the viral transfer plasmid lentiCas9-Blast (Sanjana et al., 2014) (gift from Feng Zhang; Addgene plasmid # 52962), the viral packaging plasmid psPAX2 (gift from Didier Trono ...