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Citations for Addgene :
1901 - 1950 of 2254 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Lentiviral vectors (expressing Cas9 and scramble gRNA 5’-GTGTAGTTCGACCATTCGTG or gRNA against human LC3B 5’-CATCCAACCAAAATCCCGGT (pLV[CRISPR]-hCas9:T2A:Bsd-U6>hMAP1LC3B[gRNA#954]) (Vectorbuilder) were transfected into HEK293T cells with plasmids psPAX2 (Addgene #12260) and pCMV-VSV-G (Addgene #8454 ...
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bioRxiv - Cell Biology 2024Quote: ... cell lines were generated by transfecting RPE1 cells with the plasmid pLentiCRISPRV2 (A gift from Feng Zhang, Addgene, #52961; RRID: Addgene_52961) containing the sgRNAs ...
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bioRxiv - Cell Biology 2024Quote: Viral supernatants were produced by co-transfecting HEK293T cells at 70-80% confluency in a T175 flask with 20 μg pHIV-Luc-ZsGreen (gift from Bryan Welm (Addgene plasmid #39196 ...
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bioRxiv - Cancer Biology 2021Quote: ... MOLM-13/Cas9+ cell line stably expressing luciferase was established via lentiviral infection with a Lenti-luciferase-P2A-Neo (Addgene # 105621) vector followed by G418 (1 mg/mL ...
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bioRxiv - Cancer Biology 2021Quote: HEK293T cells were transfected with plasmids encoding for Flag-p105 or Flag-p27 together with the plasmid HA-Ubiquitin (Addgene #18712). After 24h ...
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bioRxiv - Cell Biology 2020Quote: ... The hTERT-RPE1 GFP-LC3-RFP reporter cell line was generated by transducing hTERT-RPE1(Cas9) cells with retroviral particles generated with the transfer plasmid pMRX-IP-GFP-LC3-RFP (Addgene: 84573). Single cell clones were isolated and reporter functionality was tested by Torin1 and Bafilomycin A1 treatments.
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bioRxiv - Genetics 2021Quote: Lentivirus was produced by transfecting 293T cells with the gRNA and KRAB-dCas9 expression plasmid together with the packaging plasmids VsVg (Addgene 12259) and psPax2 (Addgene 12260 ...
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bioRxiv - Developmental Biology 2021Quote: For mammalian 2-hybrid assays 2.15 x 105 HEK293 or 4 x 105 A375 cells were transiently transfected with 1 µg firefly luciferase reporter plasmid (5xGAL4-TATA-luciferase, Addgene, 46756) (Sun et al ...
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bioRxiv - Biophysics 2022Quote: ... Lentivirus expressing LifeAct-GFP were produced in HEK 293T cells by cotransfecting the lentiviral plasmids pLenti.PGK.LifeAct-GFP.W (a gift from Rusty Lansford, Addgene plasmid #51010; Watertown, MA) with psPAX2 and pMD2 ...
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bioRxiv - Neuroscience 2022Quote: ... 90ul cells suspension containing 1M cells was mixed with 10 uL DNA mix: 4 ug pSpCas9(BB)-2A-Puro (PX459) plasmid (Addgene #48139), • 0.4 ug gRNA encoding plasmid (pKLV-U6gRNA(BbsI)-PGKzeo2ABFP ...
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bioRxiv - Molecular Biology 2021Quote: ... similar to how we previously generated the human cell expression constructs for AcrIIA4 and AcrIIA5 (Addgene IDs 133801 and 133802, respectively) (see Supplementary Sequences) ...
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bioRxiv - Molecular Biology 2021Quote: ... 40 million cells per replicate were activated and 12-20 h later electroporated with the pSpCas9(BB)-2A-GFP plasmid (pX458; Addgene 48138) expressing the Myc sgRNA using the MaxCyte STx transfection system (MaxCyte) ...
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bioRxiv - Cell Biology 2022Quote: Lentivirus particles were generated from HEK293T cells (ATCC CRL-3216) by co-transfection of lentiviral vectors with the packaging plasmid psPAX2 (Addgene #12260) and envelope plasmid pMD2G (Addgene #12259 ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... and Linearized pShuttle-CMV plasmids were transformed into the final viral backbone using electrocompetent AdEasier-1 cells (gift from Bert Vogelstein; Addgene, #16399). Successful incorporation of pShuttle-CMV construct into AdEasier-1 cells confirmed via digestion with PacI (ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: Lentiviral particles to generate CRISPR/Cas9-edited cell lines were produced by transfecting 10 cm dishes of HEK293T cells with 1.5 μg of pLentiCRISPRv2 encoding gene specific guide RNAs (Addgene plasmid #52961), 1 μg of p8.91 packaging plasmid(Zufferey et al. ...
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bioRxiv - Genomics 2020Quote: ... Separately infected cells were counted and pooled after selection with puromycin and KRAB-dCas9 (TRE-KRAB-dCas9-IRES-BFP, Addgene 85449) was induced with 1 μg/ml doxycycline (Millipore Sigma ...
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bioRxiv - Immunology 2021Quote: TXN1-/- HEK 293T cells were prepared by transient transfection of HEK 293T cells stably expressing Cas9 with sgRNA for TXN1 packaged in lentiGuide-Puro (Addgene, 52963) using the following oligo sequences (5’-ACGTGATATTCCTTGAAGTA-3’ ...
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bioRxiv - Cancer Biology 2021Quote: The control and LKB1-KO lines were generated by infecting the cell lines with lentivirus generated from the LentiCRISPRv2 plasmid (Addgene: 52961). The control and TPI1-KO or SIK-KO lines were generated by infecting the Cas9-expressing lines (LentiCRISPRv2 ...
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bioRxiv - Cell Biology 2021Quote: ... Lentiviruses were produced by transfecting HEK-293T cells with the transfer lentiCRISPR v2 plasmids and packaging plasmids pLTR-G (Addgene, 17532) and pCD/NL-BH*DDD (Addgene ...
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bioRxiv - Cell Biology 2021Quote: Viral particles for the generation of stable overexpressed cell lines were produced by co-transfection of pLEX_307 (a gift from David Root, Addgene plasmid # 41392) containing the target gene ...
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bioRxiv - Cell Biology 2021Quote: ... U2OS cells seeded on 35mm MatTek chambers with 70% confluency were loaded with 1 µg of smFLAG-KDM5B-24×MS2 (Addgene #81084), 0.5 µg of anti-FLAG FB-GFP and 130 ng of purified MCP-HaloTag protein by bead loading (Cialek et al. ...
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bioRxiv - Microbiology 2020Quote: Epitope-tagged expression constructs were made by first cloning cDNAs from RAW 264.7 cell RNA into pENTR1a entry vectors with indicated tags (Addgene Plasmid #17396)(Campeau et al. ...
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bioRxiv - Systems Biology 2020Quote: ... the cells were transfected with a mix of 8 μg lentiviral lentiCRISPRv2 vector containing the TKOv3 gRNA library 21 (Addgene #90294), 4.8 μg packaging vector psPAX2 ...
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bioRxiv - Neuroscience 2020Quote: ... HEK 293T cells (ATCC CRL-11268) were transfected with expression vectors for the ribozyme-flanked viral genome (cSPBN-4GFP (Addgene 52487) or pRVΔG-4tdTomato (Addgene 52500)) ...
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bioRxiv - Cancer Biology 2021Quote: ... and to the cell suspension was also added 8 ug of donor plasmid (AICSDP-52: HIST1H2BJ-mEGFP is Addgene plasmid # 109121). Cells were then electroporated using a Gene Pulser Xcell electroporation system at 160 V for 30 ms ...
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bioRxiv - Cancer Biology 2020Quote: The dCas9-KRAB constructs were transfected in 293T cells with the 2nd generation lentiviral packaging and envelope plasmids psPAX2 and pMD2.g (Addgene, USA), using CaCl2 2.5M (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: Virus particles were generated in HEK 293-T cells after transfection with the above-mentioned plasmids in combination with the gag/pol plasmid psPAX2 (Addgene, 12260) and the VSV-g-envelope plasmid pMD2.G (Addgene ...
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bioRxiv - Physiology 2022Quote: ... 60% confluent monolayers of 293-T cells were transfected with LV shuttle vector pUltra-hot-LT and the packaging plasmids psPAX2 (Addgene, 12260) and pMD2.G (Addgene ...
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bioRxiv - Genomics 2022Quote: AAV coding STAT5bCA and Luciferase (control) was prepared and quantified by triple transfection of HEK293FT cells using methods adapted from protocols provided by Addgene (www.addgene.org). HEK293FT is a fast growing and highly transfectable derivative (cat ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells seeded to 150mm dishes were transfected with 21 μg of the human gRNA pooled library in lentiGuide-Puro (Addgene #1000000049), 15.75 μg of pSPAX2 and 5.25 μg of pVSV-G plasmids ...
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bioRxiv - Microbiology 2022Quote: The full-length 3CLpro sequence of RHDV2 was codon-optimized for protein expression in mammalian cells, synthesized by Integrated DNA Technologies (Coralville, IA) and inserted into pcDNA3 H2B-mIFP T2A Mpro (3CLpro) (Addgene, MA) following digesting with BamH1 and ApaI ...
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bioRxiv - Genetics 2022Quote: ... Flox cells were grown in 24-well plates for 24h and then transfected with 130ng of H2B-GFP plasmid (Addgene, 11680). After 5h ...
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bioRxiv - Cancer Biology 2022Quote: MIA PaCa-2 wild type (WT) and TSC1/TSC2 knockout (KO) cells were transduced with retrovirus expressing RFP (pQCXIP-turboRFP, Addgene #73016) or EGFP (pQCXIP-EGFP-F ...
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bioRxiv - Immunology 2022Quote: Lentiviruses pseudotyped with HIV-1 env were prepared by transfecting the Lenti-X 293T cells with pCMV-dR8.3 Δvpr (Addgene plasmid #8455), pLOX-CW-tdTomato ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... low passage HEK293T cells were co-transfected with the corresponding pInducer20-FLAG-SMS2 construct and the packaging vectors psPAX2 (Addgene, 12260) and pMD2.G (Addgene ...
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bioRxiv - Cancer Biology 2022Quote: Stable dCas9-KRAB expressing MCF7 and SUM44 cell lines were generated by lentiviral transduction using Lenti-dCas9-KRAB-blast (Addgene #89567) transfer vector and pMD2.G (Addgene #12259 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cas9-expressing A375 cells were transduced with lentivirus containing expression plasmid pXPR-011 (gift of John Doench and David Root; Addgene #59702), which encodes for enhanced green fluorescent protein (EGFP ...
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bioRxiv - Cancer Biology 2022Quote: pLX_311-Cas9 lentivirus was produced via transfection of HEK293T cells as described above (plasmid gift of William Hahn and David Root; Addgene #118018). A375 WT cells were seeded in 12-well plates with 1 million cells per well with increasing volumes of pLX_311-Cas9 lentivirus to a final volume of 2 mL with 4 µg/mL polybrene ...
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bioRxiv - Cancer Biology 2022Quote: ... T98G Sin3B+/+ and Sin3B-/- TLR cell lines were created and then infected with lentivirus containing SceI and the template for eGFP reconstitution (Addgene #32627), after 72 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... Lentivirus constructs were also packaged in HEK-293T cells by transient transfection of the constructs in combination with the packaging constructs psPax2 (Addgene #12260) and pMΔ2.G (Addgene #12259) ...
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bioRxiv - Developmental Biology 2021Quote: Retrovirus for GFP labeling was produced from 293gp NIT-GFP packaging cells by transfection with pCMV-VSV-G plasmid (Addgene #8454). Viral particles with low titer of about 106 PFU/ml were used for infection ...
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bioRxiv - Microbiology 2020Quote: ... the SLC35B2 clones have been generated into HCT116cas9 cells that are expressing mCherry and Citrine due to integration of miReporter-PGK (Addgene#82477).
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bioRxiv - Neuroscience 2021Quote: U118-MG astrocytoma cells were transfected with the F-actin marker mCherry-LifeAct-7 plasmid (gift from Michael Davidson, Addgene #54491) using a calcium phosphate precipitation protocol.24 LifeAct-7 is a 17 amino acid peptide that binds to the actin cytoskeleton ...
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bioRxiv - Microbiology 2021Quote: ... Jurkat cells were electroporated with the pSBbi-RP-A3 and pCMV(CAT)T7-SB100 (gift from Zsuzsanna Izsvak, Addgene plasmid #34879) [69] ...
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bioRxiv - Neuroscience 2021Quote: HEK293 cells were transfected with 500 ng of equimolar pooled SCN1A_h1b sgRNAs (Table 4) and 500 ng dCas9p300Core (Addgene, plasmid #61357) using Lipofectamine 3000 ...
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bioRxiv - Neuroscience 2020Quote: ... TH-Cre hPSC cells (passage were transduced with a lentiviral construct under control of the human TH-Cre promoter (Addgene, plasmid # …..). The cells were transduced at a multiplicity of infection (MOI ...
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bioRxiv - Cancer Biology 2021Quote: Lentiviruses were made by Lipofectamine 2000-based co-transfection of 293FT cells with the respective lentiviral expression vectors and the packaging plasmids psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Developmental Biology 2022Quote: ... Lentiviruses delivering sgRNA were packed by transfecting about 70% confluent HEK293T cells cultured in T75 flasks with 5 µg pCMV-VSVG (Addgene 8454), 10 µg pCMV-dR8.2 dvpr (Addgene 8455) ...
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bioRxiv - Systems Biology 2022Quote: Lenti vectors harboring the CRISPR/Cas9 system for Arhgdia targeting were produced by co-transfection of 293FT cells with psPAX2 (Addgene, #12260), pMD2.G (Addgene ...
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bioRxiv - Cancer Biology 2020Quote: ... Cas9 was stably infected in cell lines using a lentiviral construct encoding lentiCas9-Blast (a gift from Feng Zhang, Addgene #52962). The single-guide RNAs targeting G9a and p53 listed in Table S5 were cloned into lentiGuide-Puro (a gift from Feng Zhang ...