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Citations for Addgene :
1751 - 1800 of 2254 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... SK-N-DZ cells expressing dCas9-VP64 were transduced with lentiviral particles encoding the p65-HSF1 transactivator complex (pLentiMPH2, Addgene plasmid #89308 was a gift from Feng Zhang) ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral particles were produced in HEK293T cells by transfection with 0.2 µg of pCMV-VSV-G (Addgene, #8454, MA, USA), 2 µg of psPAX2 (Addgene ...
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bioRxiv - Cell Biology 2022Quote: ... cells were cotransfected with two TALEN plasmids inducing DNA double strand breaks at the AAVS1 locus (Addgene #59025 and 59026) (González et al ...
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bioRxiv - Physiology 2020Quote: Lentiviruses were produced by transient transfection of HEK293T cells with lentiviral vectors carrying the gene of interest and pMD2.G (12259; Addgene) and psPAX2 (12260 ...
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bioRxiv - Systems Biology 2021Quote: ... Cell lines overexpressing SLC7A11 were generated using pLenti-CMV-SLC7A11-sh926R-FLAG-IRES-Hygro (a gift from William Kaelin (Addgene plasmid #118702 ...
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bioRxiv - Cancer Biology 2021Quote: ... LR1 and LR2 cell lines were then transduced with an SpCas9 lentiviral vector with a Blasticidin selection marker (Addgene #52962) and selected with Blasticidin (10ug/mL ...
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bioRxiv - Genetics 2020Quote: ... This cell line (SHK-fuGFP) was generated by transfecting a CMV-GFP_Puromycin construct (Addgene 45561, a gift from Michael McVoy) in SHK-1 cells with Fugene HD transfection reagent (Promega ...
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bioRxiv - Microbiology 2021Quote: ... and pCT-CD63-GFP (SBI, CYTO120-PA-1) plasmids were transfected in HEK293T cells with the packaging plasmids pMD2.G (Addgene; number 12259 ...
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bioRxiv - Molecular Biology 2020Quote: ... lentivirus was produced in HEK293FT cells transfected with NOCT Δ(2-15)-3F pCW57.1 and the pRSV-Rev (Addgene #12253), pMD2.G (Addgene #12259) ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentiviral particles were produced by cotransfection of 293T cells with sequence verified pINDUCER20-SATB2 or pLentiCMV_SATB2_Blast and packaging plasmids pMD2.G (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260 ...
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bioRxiv - Cell Biology 2020Quote: HeLa cells were cultured and co-transfected with the mTORC1 biosensor TORCAR (pcDNA3-TORCAR; a gift from Jin Zhang (#64927, Addgene))19 and GFP-paxillin ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells were co-transfected with pLX304 plasmids containing constructs of interest and the packaging plasmids pMD2.G (Addgene, #12259) and psPAX (Addgene ...
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bioRxiv - Genomics 2021Quote: ... one set of cells received a lentivirus expressing the Cre recombinase under the control of the hSyn1 promoter (Addgene 86641). This induced expression of the dCas9p300 transgene in the neurons ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... HEK 293T cells were transfected with lentiviral vectors and packaging vectors (pCMV-VSVG and psPAX2, Addgene # 8454 and 12260, respectively), using the Mirus TransIT-LT1 transfection reagent ...
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bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 S-Pseudotyped lentivirus27,43 were produced by co-transfection of HEK-293T cells with plasmids bearing a GFP reporter gene (pLB was a gift from Stephan Kissler, Addgene plasmid #11619 ...
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bioRxiv - Microbiology 2020Quote: ... 10cm cell culture grade petri dishes of approximately 80% confluent 293T cells were transfected with 1.4μg pMD2.G (VSV-G envelope expressing plasmid, a gift from Didier Trono (Addgene plasmid #12259), 3.6μg psPAX2 (Gag-Pol expression construct ...
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bioRxiv - Bioengineering 2021Quote: ... HEK293FT cells were cotransfected with a plasmid encoding PalmReNL and pCMV(CAT)T7-SB100 (Addgene #34879, gift from Zsuzsanna Izsvak).
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bioRxiv - Cell Biology 2020Quote: Lentiviruses were produced by MaxPEI-based transfection of HEK293T (ATCC CRL-11268) cells with the construct of interest and the packaging vectors psPAX2 and pMD2.G (Addgene). Cell supernatant was harvested 48 and 72 hrs after transfection ...
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bioRxiv - Cancer Biology 2021Quote: 293T cells (1.5 x 106 in a 10 cm Petri dish) were transfected with lentiviral plasmids together with pVSVg (Addgene, 8454) and psPAX2 (Addgene ...
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bioRxiv - Molecular Biology 2021Quote: The Rai1-HA-3x-FLAG-P2A-GFP cell line was generated by modifying a published method: a donor plasmid was constructed using the pFETCH Donor plasmid (Addgene plasmid #63934 from Eric Mendenhall and Richard M ...
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bioRxiv - Microbiology 2020Quote: The integrins subunits were overexpressed in CHO cells line with the following plasmids: Alpha 5 integrin-GFP (Addgene plasmid# 15238)50 ...
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bioRxiv - Molecular Biology 2021Quote: The pLKO.1-plasmids for REST/NRSF knockdown were co-transfected into HEK293T cells with the psPAX2 and pMD2.G helper plasmids (Addgene) using Lipofectamine 2000 (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... we first generated OsTIR1 parental cell line (Supplementary Fig. 1G, H) by transfecting pEN396-pCAGGS-Tir1-V5-2A-PuroR (Tigre donor, #92142, Addgene) and pX330-EN1201 (spCas9nuclease with Tigre sgRNA ...
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bioRxiv - Microbiology 2021Quote: ... a semi-confluent 60 mm plate was seeded with each cell line and co-transfected with 0.5 μg of pCMV(CAT)T7-SB100 (Transposase vector, Addgene # 34879) and 5 μg of pSBbi-Bla hACE2 (Transposon vector) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were co-transfected with two gRNA transcriptional cassettes prepared by PCR and CAG-Cas9-T2A-EGFP plasmid (Addgene #7831) as described elsewhere56 ...
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bioRxiv - Developmental Biology 2020Quote: ... Rcho1 cells were co-transfected with the gRNA and dCas9 expression vector (pLV hUbc-dCas9-T2A-GFP; Addgene, Plasmid #53191) [26] ...
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bioRxiv - Immunology 2021Quote: ... lentiviral particles were produced by co-transfecting 293T cells with a lentiviral plasmid encoding nano luciferase plenti-NL (a gift from Dr. Wanker through Addgene; http://n2t.net/addgene:113450 ...
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bioRxiv - Neuroscience 2021Quote: Cultured Cdh11 wild-type and knockout hippocampal neurons (300,000 cells/2 cm2) were transfected at 3 DIV or 9 DIV with the pLL3.7-GFP vector (Addgene Cat#11795) using Lipofectamine 2000 (Invitrogen Cat#11668-019 ...
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bioRxiv - Cancer Biology 2021Quote: ... the annealed oligos were ligated into plentiGuide-sgRNA-P2A-mCherry-Puro50 for all experiments in SCLC and AML cell lines or lentiCRISPR V2-Neo (Addgene #52961 where the puromycin resistance gene was replaced with the G418 resistance gene ...
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bioRxiv - Cancer Biology 2021Quote: Lentiviral constructs were also packaged in HEK-293T cells by transient co-transfection with the packaging constructs psPax2 (Addgene #12260) and pMΔ2.G (Addgene #12259) ...
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bioRxiv - Cancer Biology 2022Quote: ... PZP KTB40 and KTB42 cells were modified to express Tomato Red or GFP markers using pCDH-EF1-Luc2-P2A-tdTomato (#72486, Addgene) or pCDH-EF1-Luc2-P2A-Cop GFP (#72485 ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral constructs were packaged in 293FT cells using co-transfection of pLVX constructs with with psPAX2 and pMD2.G (Addgene). Virus titers were estimated using puromycin resistance in HEK-293T cells ...
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bioRxiv - Cancer Biology 2022Quote: ... 293T cells were transfected with retroviral or lentiviral transfer plasmid and packaging vector (retrovirus: pCL-Eco, Addgene, 12371; lentivirus: psPAX2, Addgene, 12260 with VSVg envelop plasmid pMD2.G ...
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bioRxiv - Molecular Biology 2022Quote: MCF10A-YAP5SA cells expressing Cas9-KRAB were generated by infection with lentiviral vector pHAGE TRE dCas9-KRAB (Addgene #50917; (34)) and selection with neomycin ...
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bioRxiv - Cancer Biology 2022Quote: The pLJC5-TMEM192-3xHA construct used to produce cell lines stably expressing TMEM192-3xHA was a gift from David Sabatini (Addgene plasmid # 102930 ...
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bioRxiv - Cancer Biology 2022Quote: ... we transfected SKOV3ip1 and OVCAR5 cells with ODD-luciferase-pcDNA3 construct (a kind gift from William Kaeline, Addgene plasmid # 18965) and generated stable lines 17 ...
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bioRxiv - Bioengineering 2022Quote: ... The T-cell factor/lymphoid enhancer factor (TCF/LEF) luciferase reporter SuperTopFlash (STF) and the control pRL-SV40 Renilla luciferase constructs (Addgene) were used for Wnt/β-catenin-responsive reporter assays ...
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bioRxiv - Immunology 2022Quote: Each of the recombinant plasmids was transfected into HEK293T cells together with lentiviral packaging plasmids pMD2.G and psPAX2 (gifts from Didier Trono, Addgene) using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... 3×106 cells were harvested and added to 100 µl supplemented Nucleofector Solution V containing 0.7 µg CIBN-CAAX (Addgene #79574), 1.1 µg CRY2PHRmcherry2-RASAL3 ...
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bioRxiv - Systems Biology 2022Quote: ... pEF and minCMV promoter reporter cell lines were generated by TALEN-mediated homology-directed repair to integrate donor constructs (pEF promoter: Addgene #161927 ...
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bioRxiv - Molecular Biology 2023Quote: Stimulated emission depletion microscopy was performed on U2OS cells transduced with lentivirus expressing Flag-tagged PREPL cDNA (pCIG3, Addgene #78264). Cells were seeded 3 days post-transduction on coverslips (thickness #1.5H ...
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bioRxiv - Neuroscience 2022Quote: ... High-titer lentiviral stock particles were produced in HEK293T cells by transiently co-transfecting the described plasmids with psPAX2 (#12260, Addgene) and pCMV-VSV-G (#8454 ...
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bioRxiv - Molecular Biology 2023Quote: ... ACE2 lentiviruses were filtered through a 0.45-micron filter and used to transduce respective cell lines using the reverse transduction method (Addgene, 2019). Briefly ...
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bioRxiv - Neuroscience 2023Quote: Live-cell visualization of neurons was performed by transfection using pAAV-hSyn-mCherry plasmid from Karl Deisseroth (Addgene plasmid # 114472) and Lipofectamine 3000 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: These transposon vectors were propagated in DH5α competent cells and co-transfected along with a plasmid encoding the hyperactive enzyme SB 100x Transposase (Addgene 34879 ...
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bioRxiv - Neuroscience 2022Quote: The following plasmids were used for the transfection of cultured cells: pMXs-IP-EGFP-LC3 (Hara et al., 2008) (gift from Noboru Mizushima; Addgene plasmid no ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells stably expressing Cas9 were generated by infection with the lentiCas9-Blast plasmid (Addgene # 52962, a gift from Feng Zhang). sgRNAs targeting NKX2-1 or SOX1 were selected from Brunello library (Table E2 ...
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bioRxiv - Cell Biology 2022Quote: ... Stable CHO cell lines expressing CRT-GFP or CRT(Y108F)-GFP were obtained by co-transfection with pBABE-puro (Addgene) and selection with puromycin.
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bioRxiv - Immunology 2022Quote: ... Lentiviral particles were produced in 293T cells using pMD2.G and psPAX2 (from Didier Trono, Addgene plasmids #12259 and #12260), and pINDUCER-21 p.M694V Pyrin ...
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bioRxiv - Cancer Biology 2022Quote: ... or Lifeact (Riedl et al., 2008) were transduced with viral supernatant obtained from HEK293T cells co-transfected with pCMVR8.2 (Addgene #12263) and pMD2.G (Addgene #12259 ...