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Citations for Addgene :
1901 - 1950 of 2354 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: To evaluate the NF-κB activity in the presence or absence of AF10 FPs iCALM-AF10 or iMLL-AF10 AML cells were transduced with lentivirus expressing NFkB-TA-Luc-UBC-GFP-W (Addgene plasmid 49343 ...
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bioRxiv - Cell Biology 2021Quote: Rhodopsin protein was expressed in HEK293 cells using transient transfection (pcDNA3 rod opsin construct, a gift from Robert Lucas (Addgene plasmid # 109361 ...
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bioRxiv - Biophysics 2020Quote: Murine sarcoma S-180 cells were stably transfected with plasmid construct encoding for F-TRActin-EGFP (Addgene® Plasmid #58473). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: Cell lines for use in orthotopic in vivo experiments were labeled with pLenti-PGK-V5-LUC Neo (Addgene plasmid #21471). The 293 cells were seeded in 10-cm-diameter dishes and transfected with pCMV-dR8.2 dvpr (Addgene plasmid #8455) ...
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bioRxiv - Cancer Biology 2021Quote: ... 2003)) or pCL-ECO for fetal liver cells (Addgene plasmid #12371; http://n2t.net/addgene:12371; RRID:Addgene_12371 (Naviaux et al., 1996)) ...
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bioRxiv - Cell Biology 2022Quote: ... RPE-1 cyclinB1-Venus/tubulin-mRFP cell line was generated in our lab by transduction with lentiviral vectors containing tubulin-mRFP (Addgene). RPE-1 TUB-mRFP cells were generated in our lab by transduction with lentiviral vectors containing tubulin m-RFP (Addgene) ...
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bioRxiv - Cell Biology 2022Quote: ... RPE-1 TUB-mRFP cells were generated in our lab by transduction with lentiviral vectors containing tubulin m-RFP (Addgene). HEK293T cells at a 50-70% confluence were co-transfected with lentiviral packaging vectors (16.6 μg of Pax2 ...
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bioRxiv - Molecular Biology 2022Quote: SVF cells were isolated as above and on the same day a confluent 10 cm dish of 293T cells were transfected with 1.5 µg psPAX2 lentiviral packaging vector (Addgene 12260), 1.5 µg pMD2.G envelop plasmid (Addgene ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: HEK293 cells were transfected in 96-well plates with the 8xGTIIC-luciferase plasmid (firefly luciferase, # 34615, Addgene, Watertown, MA, US) and the pRL-SVl40P plasmid (Renilla luciferase ...
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bioRxiv - Neuroscience 2022Quote: ... For the preparation of high-titer lentiviral stock lentiviral particles were produced in HEK293T cells by transiently co-transfecting the described plasmids with psPAX2 (#12260, Addgene) and pCMV-VSV-G (#8454 ...
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bioRxiv - Neuroscience 2022Quote: For HEK-cell expression, mutations were introduced in previously described ACR constructs of GtACR1 (Wietek et al., 2016) (Addgene #85464) and PhobosCA (Wietek et al. ...
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bioRxiv - Microbiology 2022Quote: Retrovirus particles for transduction and stable cell generation were produced in HNE-1 cells following JetPrime transfection of expression plasmids (pBabe neo, pBabe-HA-LMP1 neo) and packaging plasmids pMD2.G (Addgene; number 12259 ...
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bioRxiv - Cancer Biology 2022Quote: ... we transfected HEK 293T/17 cells with different plasmids together with the packaging plasmids pMD2.G (Gift from Didier Trono (Addgene plasmid #12259 ...
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bioRxiv - Cell Biology 2022Quote: ... talin 2 shRNA-stable cells were co-transfected with Tln1 siRNA and EGFP-talin 1 head (Addgene plasmid no. 32856) or EGFP-talin 1 L325R (the mutant version ...
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bioRxiv - Cancer Biology 2022Quote: MLH1 KO cell line clones were generated by transient transfection of a Cas9-T2A-EGFP expression plasmid (Addgene Plasmid #48140), with co-expression of an MLH1-targeting gRNA (5’-GCACATCGAGAGCAAGCTCC-3’) ...
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bioRxiv - Cancer Biology 2022Quote: ... and each plasmid was packaged separately in 293T cells via co-transfection with polyethylenimine alongside pCMV-VSV-G (Addgene #8454) envelope plasmid and pCMV-dR8.2 dvpr (Addgene #8455 ...
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bioRxiv - Microbiology 2021Quote: ... HEK 293T cells in 10cm dishes were transfected at 80% confluency with the lentiviral plasmid pLenti-CMV-Puro (Addgene #17452) containing the gene of interest (2 μg) ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid encoding for mitochondria specific protein/ autophagosome specific protein is transfected in HEK293T cells along with packaging vector (pDR8.2; Addgene #8455) and envelope encoding protein (VSVG ...
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bioRxiv - Microbiology 2021Quote: HeLa cells were seeded onto glass coverslips and transfected with either myc-ACE2 (pCEP4-myc-ACE2 from Addgene Plasmid #141185), ACE2 (Daly et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... SK-N-BE(2)-C cells were transduced with lentiviral constructs for stable expression of the different tested RRM2 promotor targeting sgRNAs (Addgene) (MP-I-1142 sg86RRM2 ...
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bioRxiv - Developmental Biology 2022Quote: ... The empty ctrl or MMP14-eGFP lentiviral plasmid (7.5 μg) was co-transfected into 293 cells with packaging plasmid pRSV-Rev (5 μg, Addgene #12253), pMDLg/pRRE (2.5 μg ...
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bioRxiv - Genetics 2022Quote: Cilia-APEX-IMCD3 cells were transfected with 4µg of base editing plasmid BE4-Gam (Plasmid #100806, Addgene, Watertown, Massachusetts, USA) and 1 µg of BPK1520 with respective gRNA insert using jet prime transfection reagent (Polyplus-transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... SK-N-DZ cells expressing dCas9-VP64 were transduced with lentiviral particles encoding the p65-HSF1 transactivator complex (pLentiMPH2, Addgene plasmid #89308 was a gift from Feng Zhang) ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral particles were produced in HEK293T cells by transfection with 0.2 µg of pCMV-VSV-G (Addgene, #8454, MA, USA), 2 µg of psPAX2 (Addgene ...
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bioRxiv - Cell Biology 2022Quote: ... cells were cotransfected with two TALEN plasmids inducing DNA double strand breaks at the AAVS1 locus (Addgene #59025 and 59026) (González et al ...
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bioRxiv - Cancer Biology 2019Quote: ... the HFF-1 cells were lentivirally transduced with GFP-H2B nuclear marker using the PGK-H2BeGFP system as described by Addgene and selected by flow sorting for GFP positive cells.
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bioRxiv - Microbiology 2019Quote: The lentiviral expression vectors pLEX-GFP and pLEX-YTHDF2 were transfected into 293T cells along with the packaging plasmids pMD2G (12259; Addgene) and pΔ8.74 (22036 ...
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bioRxiv - Molecular Biology 2019Quote: ... were then inserted into the AAVS1 locus of HCT116 CRISPRi cells using a previously described TALEN pair targeting the AAVS1/PPP1R12C locus (Sanjana et al., 2012) (hAAVS1 1L TALEN, Addgene plasmid #35431 ...
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bioRxiv - Cell Biology 2019Quote: ... The cells were transduced first by lentivirus expressing dCas9 fused to the KRAB repressor module9 (pHR-SFFV-dCas9-BFP-KRAB, Addgene). Lentivirus-expressing cells were sorted (FACSAria II ...
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bioRxiv - Cancer Biology 2019Quote: ... SENP8) clonal Cas9 expressing BC-3 cells were transduced with lentiviral sgRNA vectors based on pLenti-guide puro (Addgene #52963)34 ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293T/17 cells were cotransfected with the respective transfer vector and second-generation lentiviral cassettes (packaging vector psPAX2 (Addgene RRID:Addgene_12260) and envelope vector pMD2.G (Addgene RRID:Addgene_12259) ...
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bioRxiv - Physiology 2020Quote: Lentiviruses were produced by transient transfection of HEK293T cells with lentiviral vectors carrying the gene of interest and pMD2.G (12259; Addgene) and psPAX2 (12260 ...
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bioRxiv - Systems Biology 2021Quote: ... Cell lines overexpressing SLC7A11 were generated using pLenti-CMV-SLC7A11-sh926R-FLAG-IRES-Hygro (a gift from William Kaelin (Addgene plasmid #118702 ...
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bioRxiv - Cancer Biology 2021Quote: ... LR1 and LR2 cell lines were then transduced with an SpCas9 lentiviral vector with a Blasticidin selection marker (Addgene #52962) and selected with Blasticidin (10ug/mL ...
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bioRxiv - Genetics 2020Quote: ... This cell line (SHK-fuGFP) was generated by transfecting a CMV-GFP_Puromycin construct (Addgene 45561, a gift from Michael McVoy) in SHK-1 cells with Fugene HD transfection reagent (Promega ...
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bioRxiv - Microbiology 2021Quote: ... and pCT-CD63-GFP (SBI, CYTO120-PA-1) plasmids were transfected in HEK293T cells with the packaging plasmids pMD2.G (Addgene; number 12259 ...
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bioRxiv - Molecular Biology 2020Quote: ... lentivirus was produced in HEK293FT cells transfected with NOCT Δ(2-15)-3F pCW57.1 and the pRSV-Rev (Addgene #12253), pMD2.G (Addgene #12259) ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentiviral particles were produced by cotransfection of 293T cells with sequence verified pINDUCER20-SATB2 or pLentiCMV_SATB2_Blast and packaging plasmids pMD2.G (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260 ...
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bioRxiv - Cell Biology 2020Quote: HeLa cells were cultured and co-transfected with the mTORC1 biosensor TORCAR (pcDNA3-TORCAR; a gift from Jin Zhang (#64927, Addgene))19 and GFP-paxillin ...
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bioRxiv - Systems Biology 2019Quote: ... the cells were transfected with a mix of 8 µg lentiviral lentiCRISPRv2 vector containing the TKOv3 gRNA library (Addgene #90294) (Hart et al ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells were co-transfected with pLX304 plasmids containing constructs of interest and the packaging plasmids pMD2.G (Addgene, #12259) and psPAX (Addgene ...
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bioRxiv - Genomics 2021Quote: ... one set of cells received a lentivirus expressing the Cre recombinase under the control of the hSyn1 promoter (Addgene 86641). This induced expression of the dCas9p300 transgene in the neurons ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... HEK 293T cells were transfected with lentiviral vectors and packaging vectors (pCMV-VSVG and psPAX2, Addgene # 8454 and 12260, respectively), using the Mirus TransIT-LT1 transfection reagent ...
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bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 S-Pseudotyped lentivirus27,43 were produced by co-transfection of HEK-293T cells with plasmids bearing a GFP reporter gene (pLB was a gift from Stephan Kissler, Addgene plasmid #11619 ...
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bioRxiv - Microbiology 2020Quote: ... 10cm cell culture grade petri dishes of approximately 80% confluent 293T cells were transfected with 1.4μg pMD2.G (VSV-G envelope expressing plasmid, a gift from Didier Trono (Addgene plasmid #12259), 3.6μg psPAX2 (Gag-Pol expression construct ...
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bioRxiv - Bioengineering 2021Quote: ... HEK293FT cells were cotransfected with a plasmid encoding PalmReNL and pCMV(CAT)T7-SB100 (Addgene #34879, gift from Zsuzsanna Izsvak).
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bioRxiv - Cell Biology 2020Quote: Lentiviruses were produced by MaxPEI-based transfection of HEK293T (ATCC CRL-11268) cells with the construct of interest and the packaging vectors psPAX2 and pMD2.G (Addgene). Cell supernatant was harvested 48 and 72 hrs after transfection ...
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bioRxiv - Cancer Biology 2021Quote: 293T cells (1.5 x 106 in a 10 cm Petri dish) were transfected with lentiviral plasmids together with pVSVg (Addgene, 8454) and psPAX2 (Addgene ...
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bioRxiv - Molecular Biology 2021Quote: The Rai1-HA-3x-FLAG-P2A-GFP cell line was generated by modifying a published method: a donor plasmid was constructed using the pFETCH Donor plasmid (Addgene plasmid #63934 from Eric Mendenhall and Richard M ...
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bioRxiv - Microbiology 2020Quote: The integrins subunits were overexpressed in CHO cells line with the following plasmids: Alpha 5 integrin-GFP (Addgene plasmid# 15238)50 ...