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Citations for Addgene :
1651 - 1700 of 2354 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... Viral particles were produced in HEK293T packaging cell line by triple transfection with psPAX2 (Addgene #12260; http://n2t.net/addgene:12260; RRID:Addgene_12260) and pMD2G (Addgene #12259 ...
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bioRxiv - Bioengineering 2020Quote: ... A ChR2-expressing Spiking HEK cell line was created by Lipofectamine transfection with pcDNA3.1/hChR2(H134R)-EYFP (Addgene, Watertown ...
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bioRxiv - Cell Biology 2021Quote: The following plasmids were used to produce viruses for the various transgenic cell lines: lentiviral plasmid (Addgene #19119), dR8.2 packaging plasmid (Addgene #8455) ...
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bioRxiv - Cancer Biology 2020Quote: ... were purchased from Sigma and lentiviral partilces were generated in HEK293T cells by contransfecting shRNA plasmid construct with pMD2.G (Addgene#12259) and psPAX2 (Addgene#12260) ...
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bioRxiv - Cancer Biology 2020Quote: ... as well as B16F10 and PANO2 cells overexpressing custom-made plasmid encoding KrasG12C(pKrasG12C; Addgene #64372; GFP-KrasG12C_2B_puro) were produced as described elsewhere (Agalioti et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... H357 and SCC-9 cells were transfected with RRBP1 overexpression plasmids pcDNA4 HisMax-V5-GFP-RRBP1(Addgene:Cat#92150) using the ViaFect transfection reagent (Promega Cat# E4982) ...
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bioRxiv - Neuroscience 2020Quote: ... A CAD5 cell line stably expressing Cas9 (CAD5-Cas9) was generated by transfecting linearized pCMV-hCas9 (Addgene 41815,). CAD5-Cas9 cells were then transfected with pKLV-Prnp sgRNA by Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were co-transfected with lentiviral constructs for overexpression with the packaging vectors Δ8.9 and VSV-G (Addgene) using FuGENE®-6 (Promega) ...
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bioRxiv - Biochemistry 2021Quote: ... CRISPR Jurkat T cells were generated by stably expressing with lentiviral particles pLX-311-Cas9 construct (Addgene 96924) and transiently transfecting with Amaxa® Cell Line Nucleofector® Kit T (Ref ...
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bioRxiv - Systems Biology 2020Quote: HEK293T cells were transfected with the combined A and B components of the GeCKO v2 (Addgene #1000000048, #1000000049) whole genome library (123,411 sgRNAs in total ...
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bioRxiv - Genomics 2020Quote: Lentiviral particles were produced in HEK293T cells following transient transfection of the packaging plasmids pVSVg (Addgene plasmid #8454) and psPAX2 (Addgene plasmid #12260) ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... 293FT cells were co-transfected with Cas9-expressing plasmid lentiCas9-Blast (Addgene #52962, a gift from Feng Zhang), the lentiviral packaging plasmid psPAX2 (Addgene #12260 ...
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bioRxiv - Cell Biology 2021Quote: ... These parental BV2 or BV2-Cas9 cells were transduced for 2 days with pXPR_011 expressing eGFP (Addgene; 59702) and a short guide RNA (sgRNA ...
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bioRxiv - Cancer Biology 2021Quote: ... STING knockout cell lines were generated using GFP and RFP lentiviral vectors (Addgene, ref 57818 and ref 57819) in which the following guild-RNA were cloned ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were transfected with 0.45 μg of the indicated constructs and 0.05 µg of tRNA/synthetase50 (Addgene number ...
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bioRxiv - Cell Biology 2021Quote: ... U2OS dCas9-KRAB cells were generated by lentiviral transduction with pMH0006 (Addgene, cat# 135448; Chen et al., 2019) and selected for BFP expression by FACS ...
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bioRxiv - Cell Biology 2020Quote: Stably Cas9-expressing HepG2 cells were transduced with genome-wide lentiviral pooled CRISPR/Cas9 knockout library (Addgene #73178) (Brunello ...
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bioRxiv - Cell Biology 2021Quote: ... Virus particles were produced in HEK293FT (RRID_6911) cells by co-transfection of AAV plasmids with the helper plasmid pAdDeltaF6 (RRID:Addgene_112867) and AAV packaging plasmid expressing Rep2/Cap5 genes for production of serotype 5 – pAAV2/5 (RRID:Addgene_104964).
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bioRxiv - Cancer Biology 2022Quote: ... 90% confluent HEK29T3 cells in 100 mm plates were transfected with pMD2.G (1.5 μg; Addgene plasmid # 12259), psPAX2 (3.0 μg ...
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bioRxiv - Cell Biology 2022Quote: ... H9c2 cells seeded in 96 well plates were co-transfected with reporter plasmid and pcDNA3-Mettl3 (Addgene, #53739) (100 ng for each ...
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bioRxiv - Cancer Biology 2022Quote: ... OP9 cells were transduced with GFP lentivirus (derivative of pRRL-GFP vector from Dr Trono group, Addgene 12252). OP9 labelling with GFP allowed for differentiation of OP9 cells from leukemia cells in flow cytometry experiment ...
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bioRxiv - Biochemistry 2022Quote: ... Lentivirus was generated by transfection of HEK293T cells with expression construct plasmid DNA with pMDLg/pRRE (Addgene, 12251), pRSV-Rev ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transduced using a lentiviral packaging system with a Cas9 expressing vector (pKLV2-EF1a-Cas9Bsd, Addgene #68343) and Cas9 activity was measure using two different Cas9 reporters (pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP ...
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bioRxiv - Biochemistry 2022Quote: ... NIH 3T3 cells were transfected with M50 Super 8xTOPFlash reporter (Addgene plasmid #12456; http://n2t.net/addgene:12456; RRID:Addgene_12456) or M51 Super 8xFOPFlash reporter (Addgene plasmid #12457 ...
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bioRxiv - Biochemistry 2022Quote: HEK293T cells were transiently transfected with pGP-CMV-GcAMP6s (Ca2+ Sensor plasmid, Addgene, Cat. no. 40753; 4 µg), 5HT2c receptor (as positive control ...
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bioRxiv - Cancer Biology 2022Quote: Lentivirus was produced using 293T cells with the second-generation packaging system psPAX2 (#12260, Addgene, Watertown, MA, USA) and PMD2 ...
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bioRxiv - Neuroscience 2021Quote: ... 450nl of a mostly anterograde virus containing the Cre recombinase under CamKII promoter to target pyramidal cells (AAV1_CamKII_Cre_SV40, Addgene, USA) was injected into the right MEC (+3.2mm laterally from Lambda along the lambdoid suture and DV -2.5mm from skull level) ...
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bioRxiv - Cancer Biology 2020Quote: AsPC-1 and Panc 08.13 cells were transfected with pDRGFP plasmid (a gift from Maria Jasin; Addgene #26475)(82) ...
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bioRxiv - Cancer Biology 2020Quote: ... GFP-expressing TSC cells were established after transduction with lentivirus carrying the pUltra (gift from Malcolm Moore (Addgene plasmid # 24129 ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293 cells grown in twelve 10 cm dishes were co-transfected with active myc-mTOR E2914K mutant (Addgene) and HA-Raptor (Kim et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... Linearized targeting constructs (2µg) were electroporated into AB2.2 ES cells with 20µg of a pX330 plasmid (Addgene # 42230) expressing Cas9 and a sgRNA sequence to target the ROSA26 locus just inside the 5’ homology arm ...
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bioRxiv - Immunology 2019Quote: ... Lentivirus was generated by calcium phosphate transient transfection of 293T cells using psPAX2 packaging (Addgene, Cambridge, MA, USA) and pMD2.G (Addgene ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were co-transfected with siRNA and 1 µg of plasmid encoding Perceval HR (Addgene ID:49083) at around 50% cell confluency ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentivirus was produced by transfection of HEK293T cell line with psPAX2 (a gift from Didier Trono, Addgene #12260), pMDG.2 (a gift from Didier Trono ...
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bioRxiv - Synthetic Biology 2020Quote: Lentivirus production was performed using HEK-293FT cells and second-generation helper plasmids MD2.G (Addgene plasmid #12259) and psPax2 (Addgene plasmid #12260) ...
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bioRxiv - Microbiology 2019Quote: ... 1.5 million fibroblast cells were transfected with the homologous recombination donor plasmid and an helper plasmid (Addgene #64221) (21) ...
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bioRxiv - Cell Biology 2019Quote: HT1080 cells were transfected with Active WNT3A-V5 (G-8) and Active WNT4-V5 (G-10) from Addgene kit #1000000022 ...
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bioRxiv - Cancer Biology 2020Quote: D10 and SK-MEL-23 melanoma cells were first transduced to stably express Cas9 (lentiCas9-Blast, Addgene, #52962) and selected with blasticidin (5 μg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... The J8 clonal cell line was infected with lentiCRISPR v2 (a gift from Feng Zhang; Addgene plasmid # 52961), lacking or containing guide RNA against BCAR3 ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa R19 cells stably expressing mCherry-EGFP-LC3 were obtained through stable transfection of plasmid DNA (Addgene #22418), followed by puromycin selection and generation of monoclonal cell populations by limited dilution ...
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bioRxiv - Cell Biology 2021Quote: Measurement of NFAT4 nuclear translocation in HEK293 cells was achieved by transient overexpression of NFAT4-GFP (Addgene #21664) as previously described(30 ...
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bioRxiv - Cell Biology 2020Quote: MCF10A cells were transfected via PEI (https://www.addgene.org/protocols/transfection/) with pSpCas9(BB)-2A-GFP (PX458) - (Addgene #48138) carrying sgRNA for hSLC9A1 KO 5’-GTTTGCCAACTACGAACACG (SLC9A1:HGLibA_45399 ...
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bioRxiv - Biochemistry 2021Quote: ... Electrocompetent BW25113 cells were co-transformed with the pORTMAGE-4 plasmid (Addgene plasmid #72679, courtesy of Csaba Pál) and the pBAD-yTrm5 plasmid ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells at 70% confluence were transfected with 10 μg of pMRX-IP-GFP-LC3-RFP (#84573, Addgene), 5 μg of VSV.G (#14888 ...
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bioRxiv - Neuroscience 2021Quote: AAV1/2.hSyn-GFP particles were generated by co-transfection of HEK293T cells with AAV2/1 (Addgene 112862), AAV2/2 (Addgene 104963) ...
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bioRxiv - Immunology 2021Quote: ... the RBD expression vectors were co-transfected into Expi293F cells together with the secreted BirA-Flag plasmid (Addgene) in the presence of 100 μM biotin.
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bioRxiv - Genomics 2021Quote: ... These cells were co-transfected with lentiviral packaging plasmids like VSVG (a gift from Bob Weinberg, Addgene #8454) and PAX2 (a gift from Didier Trono ...
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bioRxiv - Cell Biology 2020Quote: ... Linearized pShuttle-CMV plasmids were transformed into the final viral backbone using electrocompetent AdEasier-1 cells (Addgene, #16399). Successful incorporation of pShuttle-CMV construct into AdEasier-1 cells confirmed via digestion with PacI (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... were injected with a viral vector specifically targeting GCaMP6f expression to CaMKII-positive cells (AAV5.CamKII.GCaMP6f.WPRE.SV40, Penn Vector Core via Addgene, CS1024). GCaMP6f injections were targeted at the following coordinates ...
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bioRxiv - Biophysics 2019Quote: ... SUM-159 cells genome edited to express σ2-EGFP [2] and transiently expressing mRuby-CLTB (Addgene; Plasmid #55852) were cultured in F-12 medium with hydrocortisone ...