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Citations for Addgene :
1501 - 1550 of 2354 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: The KO cell lines were generated by co-transfecting pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene 42230) with two sgRNAs (sequences see table below) ...
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bioRxiv - Synthetic Biology 2024Quote: Ligands were cloned into a pcDNA backbone to confer high expression in HEK293FT cells (Addgene #138749)26 ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviruses were produced using a similar procedure with 293T cells using the psPAX2 plasmid (Addgene, 12260) instead of pUMVC.
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bioRxiv - Microbiology 2023Quote: ... cells were co-transfected with 0.5 μg of pCMV(CAT)T7-SB100 (transposase vector; Addgene, 34879) and 5 μg of pSBbi or pSBtet containing gene of interest using TransIT-LT1 transfection reagent (Mirus Bio) ...
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bioRxiv - Molecular Biology 2023Quote: ... with 3 million K562 cells and 10 µg of pCMV-PE2-tagRFP-BleoR (Addgene no. 192508) per individual electroporation (Day 0) ...
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bioRxiv - Cancer Biology 2023Quote: ... The control and ERBB2 OE cells were generated using retroviral vectors pBABE-puro (Addgene, cat# 1764) and pBABEpuro-ERBB2 (Addgene ...
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bioRxiv - Bioengineering 2023Quote: HEK293T cells were transfected with ACE2- and TMPRSS2-expression plasmids (RRID: Addgene_141185 and RRID: Addgene_145843, respectively) to allow infection by pseudo-viruses as previously described [12] ...
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bioRxiv - Cancer Biology 2023Quote: ... 293FT cells were co-transfected using (per condition) 3 µg of pLKO.GFP transfer plasmid (Addgene, #30323) containing the shRNA (sequences in Supplementary Table S6) ...
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bioRxiv - Immunology 2023Quote: ... mouse naïve CD4+ T cells were transfected with p8xEGFP-N1 (Addgene # 122168, gift from Georg Mayr) which expresses 8 concatemeric EGFP proteins ...
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bioRxiv - Genetics 2023Quote: Prime editing experiments in K562 and Jurkat cell lines were performed with pCMV-PEmax (Addgene 174820). The ATP1A1-Q118R_v2 epegRNA11 was cloned into pU6-tevopreq1-GG-acceptor (Addgene 174038 ...
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bioRxiv - Genetics 2024Quote: ... After 24h cells were transfected with 8,750ng of indicated vector and 8,750ng of psPAX2 (Addgene#12260) and 875ng of pMD2.G (Addgene#12259 ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then reinfected on DIV 10 with AAV9-mCherry (pENN.AAV9.CB7.CI.mCherry.WPRE.RBG, Addgene #105544-AAV9). Cells were fixed on DIV 18 and endogenous mCherry fluorescence was imaged using a Nikon C2 scanning confocal microscope ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were transduced with lentivirus generated from plasmid UCOE-SFFV-dCas9-BFP-KRAB (Addgene plasmid #85969) as described above ...
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bioRxiv - Cancer Biology 2024Quote: ... cell lines were transiently transfected with a membrane-targeted fluorescent protein (glycosylphosphatidylinositol-anchored eGFP, Addgene #32601) using a TransIT-X2 transfection kit (Mirus) ...
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bioRxiv - Cancer Biology 2024Quote: Each pool of the library was transduced into MeWo melanoma cells stably expressing rtTA (Addgene #26429) using conditions that predominantly lead to a single retroviral integration and represent each shRNA in a calculated number of 9,600 cells (a total of 1.2 million cells at infection ...
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bioRxiv - Immunology 2024Quote: ... HEK293T cells were transfected with the lentiviral vector pScalps-EGFP-Cre recombinase (7) (Addgene plasmid 207132) together with the packaging vectors psPAX2 and pMD2.G (Addgene plasmids 12260 and 12259 by Didier Trono ...
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bioRxiv - Evolutionary Biology 2024Quote: ... MYO3 and MYO5 SH3-deleted yeast competent cells were co-transformed with pCAS (Addgene plasmid 60847) containing the stuffer-specific gRNA and Streptococcus pyogenes Cas929 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... MYO3 and MYO5 SH3-deleted yeast competent cells were co-transformed with pCAS (Addgene plasmid 60847) containing the stuffer-specific gRNA and Streptococcus pyogenes Cas9 with the PCR amplified MYO3 and MYO5 SH3 single position mutated libraries from the pUC19 preparations acting as the donor DNA29 ...
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bioRxiv - Immunology 2024Quote: Stably expressing H2B-GFP RPE1 WT and SP110-/- cells were generated using lentivirus generated from Addgene plasmid #91788 (Courtesy of Beverly Torok-Storb) ...
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bioRxiv - Cancer Biology 2024Quote: ... After 32 hours cells were transfected with an equimolar mix of CK2α/β plasmid (Addgene; #27093) and the relevant MCAM tail variant ...
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bioRxiv - Cancer Biology 2021Quote: ... MCF7 and MCF7-F cells were infected with pLenti-CMV-puro-Luc lentiviral luciferase construct (Addgene 17477) to monitor tumor growth ...
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bioRxiv - Molecular Biology 2019Quote: ... dCas9-expressing cells were then infected with the MS2-P65-HSF1 activator helper complex (Addgene, 61426-LVC) and treated with 0.5 mg/ml hygromycin ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected by JetPEI (Ozyme) according to manufacturer’s instructions with either an empty px330 plasmid (Addgene) coding only for Cas9 or a px330 plasmid coding for Cas9 and a sgRNA targeting rDNA [13] ...
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bioRxiv - Immunology 2021Quote: ... GL261-OVA was generated by transducing parent cells with ovalbumin cloned from pcDNA3-OVA (Addgene plasmid # 64599). Transduction was performed as described (85) ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were transfected with 4µg shRNA p53-puromycin (Fachinetti Lab) + 4µg pMD2.G (12259 from Addgene, RRID:Addgene_12259) + 4µg psPAX2 (12260 from Addgene ...
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bioRxiv - Cell Biology 2022Quote: ... new lentiviral particles were produced by transfecting HEK293 cells with 4µg Apple-53BP1trunc (69531 from Addgene, RRID:Addgene_69531) + 4µg pMD2.G (12259 from Addgene ...
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bioRxiv - Cell Biology 2022Quote: ... new lentiviral particles were produced by transfecting HEK293 cells with 4µg Apple-53BP1trunc (69531 from Addgene, RRID:Addgene_69531) + 4µg pMD2.G (12259 from Addgene ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transiently transfected with pRK5-myc-Rac1-Q61L (Addgene plasmid # 12983; http://n2t.net/addgene:12983; RRID:Addgene_12983) with Lipofectamine (ThermoFisher ...
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bioRxiv - Molecular Biology 2020Quote: ... Each sgRNA lentiviral construct was transfected into HEK 293T cells along with packaging vectors psPAX2 (Addgene #12260) and pCMV-VSV-G (Addgene #8454 ...
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bioRxiv - Cell Biology 2022Quote: ... WT and FIP200 KO HeLa cells were cotransfected with the DNA fragment and the PX459 (Addgene #48139)-based plasmid expressing Cas9 and gRNA (GTCTTAGAAGCCGTGAGGTA for the NCOA4 gene ...
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bioRxiv - Microbiology 2020Quote: ... the mouse cDNA sequence of IRE1 from MEF cells was inserted into pcDNA3- EGFP plasmid (Addgene #13031), resulting in fusion proteins with the EGFP fused to the carboxy terminus of IRE1 ...
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bioRxiv - Molecular Biology 2020Quote: ... For expression in Drosophila S2 cells (68) the mtSSB coding sequence was cloned into pMT-puro (Addgene) using a two-step procedure ...
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bioRxiv - Cell Biology 2020Quote: ... the Cas9 and mCherry for detection of electroporated cells were obtained from Addgene (plasmids #66939, #66940, #66941). All targeting constructs (for both HDR and NHEJ ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were transduced with lentiviral particles produced using vector pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS (Addgene #60904 ...
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bioRxiv - Molecular Biology 2019Quote: ... 10cm plate) cells were transfected using PEI with psPAX2 (3.5µg, a gift from Didier Trono, Addgene #12260), pMD2.G (1.5µg ...
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bioRxiv - Molecular Biology 2019Quote: ... K562 cells were transduced with EZ-Tet-pLKO-Hygro (a gift from Cindy Miranti, Addgene plasmid #85972) containing either HMG20A- ...
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bioRxiv - Molecular Biology 2019Quote: ... 3T3 cells were transfected with the gRNA-expressing plasmids and the Cas9-expressing plasmid hCas9 (Addgene #41815) using Lipofectamine LTX reagent (Thermo Fisher #15338100 ...
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bioRxiv - Cancer Biology 2019Quote: U2OS-EJ7 cells were infected with viruses produced in HEK293T using second generation vectors psPAX2 (Addgene #12259) as packaging plasmid ...
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bioRxiv - Bioengineering 2019Quote: GV-expressing cells were produced by transforming a pET28a plasmid containing the arg1 gene cluster12 (Addgene #106473) into BL21(A1 ...
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bioRxiv - Cell Biology 2019Quote: ... AICSDP-1:PXN-EGFP was a gift from The Allen Institute for Cell Science (Addgene plasmid 87420).
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bioRxiv - Bioengineering 2020Quote: ... either of the two cell types was subjected to Lipofectamine transfection with CMV-R-GECO1.2-mCherry (Addgene, Watertown ...
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bioRxiv - Bioengineering 2020Quote: VSV-G pseudotyped lentivirus was produced via transfection of HEK 293T cells (ATCC) using psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were infected to express genetically encoded multimeric nanoparticles (GEMs) using lentiviral construct from Addgene (Plasmid #11693416). Cells were FACS sorted to purify population of cells expressing GEMs.
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bioRxiv - Cell Biology 2021Quote: HEK-293T knockout cell lines were generated by transient transfection of pSpCas9(BB)-2A-Puro (Addgene 48139) encoding U6 driven expression of sgRNAs (Scramble Guide ...
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bioRxiv - Microbiology 2020Quote: Huh7.5.1-Cas9 cells were generated by lentiviral transduction with lentiCas9-blast (Addgene, #52962, gift from Feng Zhang) and subsequently selected with blasticidin for 7 days ...
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bioRxiv - Microbiology 2021Quote: ... A375-dCas or HeLa-dCas cells were generated by transducing with pLenti-dCas9-VP64-Blast (Addgene, #61425). After 7 days of blasticidin selection ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by transformation into competent cells according to an online protocol (GeCKO library Amplification Protocol from Addgene). Afterwards ...
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bioRxiv - Cell Biology 2020Quote: ... pLKO plasmids were co-transfected into HEK293-T cells along with packaging plasmids pMDLg/pRRE (Addgene #12251), pRSV-Rev (Addgene #12253 ...
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bioRxiv - Cell Biology 2021Quote: ... NCI-H1573 and NCI-H1299 cells were transfected with LeGO lentiviral particles (Addgene plasmids #27338, #27339, #27340) (Weber et al. ...
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bioRxiv - Cancer Biology 2022Quote: T98GIRF3-/- and T98GCTRL cell lines were generated using the LentiCRISPRv2GFP plasmid (gift from David Feldser; Addgene # 82416). IRF3 or control guide RNAs were inserted into the LentiCRISPRv2GFP plasmid following provided guidelines and lentiviral particles generated ...