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Citations for Addgene :
1451 - 1500 of 2354 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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AGS3 antagonizes LGN to balance oriented cell divisions and cell fate choices in mammalian epidermisbioRxiv - Developmental Biology 2022Quote: ... Packaging of lentivirus was performed using 293FT cells and pMD2.G and psPAX2 helper plasmids (Addgene plasmids #12259 and #12260 ...
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bioRxiv - Cancer Biology 2022Quote: Cas9 expressing cells were created by transduction of lentiCas9-Blast (a gift from Feng Zhang, Addgene plasmid #52962 ...
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bioRxiv - Cancer Biology 2022Quote: ... HS-SY-II and SYO1 synovial sarcoma cell lines were transduced with lentiCas9-Blast85 (Addgene, #52962) and selected using 20 μg/ml of blasticidin to generate stable Cas9-expressing cell lines ...
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bioRxiv - Molecular Biology 2019Quote: K562 cells were transfected with M_ST1n_VPR (0.25 µg) (aka dSt1Cas9-VPR) (Chavez et al., 2015) (Addgene plasmid #63799 ...
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bioRxiv - Cancer Biology 2019Quote: ... HCT116-Dnmt1Δ3-5 cells were transfected with pcDNA3 vector containing WT full length DNMT1 (36939, AddGene) and empty pcDNA3 vector as a transfection control (10792 ...
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bioRxiv - Molecular Biology 2020Quote: 1×106 cells of WTC p42 were transfected with 5mg AAVS1-TALEN R plasmid (Addgene #59026), 5 μg AAVS1-TALEN L plasmid (Addgene #59025) ...
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bioRxiv - Cancer Biology 2020Quote: ... wild-type HeLa and MCF10A cells were infected with the dCas9 lentiviral construct (Addgene 61425-LV) and selected with 3μg/ml blasticidin for 5 days ...
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bioRxiv - Immunology 2020Quote: ... and lentiviral particles were generated in 293T cells (ATCC) using packaging plasmids pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260 ...
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bioRxiv - Cancer Biology 2020Quote: ... Blasticidin-resistant cells were subsequently transduced with viral supernatant containing LentiGuide-Puro plasmid (Addgene plasmid #52963) with ligated selected sgRNA ...
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bioRxiv - Cancer Biology 2019Quote: ... H2B-RFP stable cell lines were generated after transfection with lentiviral construct H2B-RFP (Addgene 26001) and sorting for RFP-positive cells using flow cytometry ...
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bioRxiv - Microbiology 2021Quote: ... subconfluent HEK293T cells were cotransfected with lentiviral genome (psPAX2) (gift from Didier Trono (Addgene plasmid # 12260), with an envelope coding plasmid (pMD2G-VSVG or SARS-CoV-2 Spike protein (HDM_IDTSpike_fixK (kind gift from Bloom’s laboratory ...
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bioRxiv - Biochemistry 2020Quote: ... cell transfection method as described previously (Purroy et al., 2018) using the plasmids pMD2.G (RRID:Addgene_12259) and psPAX2 (RRID:Addgene_12260 ...
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bioRxiv - Cell Biology 2021Quote: Early passage HeLa-M cells were transfected with 1.5 µg of px459 plasmid (Addgene plasmid #62988) containing a single guide RNA (sgRNA ...
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bioRxiv - Biophysics 2021Quote: ... BL21 AI cell was co-transformed with MutS-bio and BirA expression plasmid (Addgene plasmid #20857)70 and was grown in LB containing 50 μg/ml kanamycin ...
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bioRxiv - Cancer Biology 2021Quote: Cell lines for use in orthotopic in vivo experiments were labeled with pHIV-luc-ZsGreen (Addgene). Bioluminescent imaging was performed every 3-7 days following intraperitoneal injection of D-luciferin and measured using the Xenogen IVIS Spectrum in vivo imaging system (PerkinElmer) ...
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bioRxiv - Biophysics 2020Quote: ... HEK293T cells on the wells were co-transfected with 1250 ng of ACE2 (Addgene plasmid #141185) and 1250 ng of TMPRSS2 expression plasmids (Addgene plasmid #145843 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with a pCMV-eNpHR3.0-EYFP plasmid derived from pCaMKIIa-eNpHR3.0-EYFP (Addgene #26971). For this construct ...
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bioRxiv - Microbiology 2020Quote: ... The sgRNA plasmid library was packaged in 293FT cells after co-transfection with psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... the cells were transduced by EF1a-CasRx (no NLS-RfxCas13d)-2A-EGFP (modified from Addgene #109049) lentivirus ...
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bioRxiv - Developmental Biology 2022Quote: Lentivirus was generated in HEK293T cells using a second-generation system with pMD2.G (Addgene 12259) and psPAX2 (Addgene 12260) ...
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bioRxiv - Cancer Biology 2022Quote: ... HCT116 cells were transfected with the HDR template and pCAG-SpCas9-GFP-U6-gRNA (Addgene: 79144) expressing a gRNA targeted near the stop codon of PGC-1β ...
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bioRxiv - Cancer Biology 2022Quote: All lentiviral particles were generated by co-transfecting HEK293T cells with 3.25 μg pCMVR8.2 (Addgene #12263) and 1.7 μg pMD2.G (Addgene #12259 ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293T cells were transfected with lentiviral plasmid and the second-generation packaging plasmids psPAX2 (Addgene, #12260) and pMD2.G (Addgene ...
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bioRxiv - Cancer Biology 2022Quote: ... PC-3 HRE luciferase cells were generated by co-transfection of 3xHRE-luciferase construct (Addgene 26371) with a puromycin-resistant construct ...
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bioRxiv - Cell Biology 2022Quote: Luciferase assays were performed in HEK293T cells using the YAP/TAZ reporter 8xGTIIC-lux (Addgene, 34615). Renilla-Luc (0.05 µg/well ...
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bioRxiv - Cell Biology 2022Quote: ... cell cultures were transfected at 50% confluency with mEmerald-Tomm20-C-10 plasmid DNA (Addgene, 54281), and imaged one day after transfection ...
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bioRxiv - Genomics 2022Quote: ... Inducible knock-out cell lines were produced with the TLCV2 CRISPR-Cas9 backbone (Addgene no. 87360) that includes a doxycycline-inducible Cas9-2A-eGFP cassette ...
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bioRxiv - Microbiology 2022Quote: Transfection of 24ST1NLESG cells was carried out with 8 μg PAK1 expression plasmid (Addgene, cat # 12208), PAK2 expression plasmid (Sino Biologicals ...
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bioRxiv - Immunology 2022Quote: ... HEK 293T cells were co-transfected with equal amounts of the pspgRNA plasmids and pCas9_GFP (Addgene plasmid #44719 ...
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bioRxiv - Systems Biology 2023Quote: Jurkat cells expressing dCas9-KRAB fusion protein were constructed by lentiviral delivery of pMH0006 (Addgene #135448) and FACS isolation of BFP-positive cells.
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bioRxiv - Systems Biology 2023Quote: ... The control was Eahy926 cells transfected with a control plasmid (a gift from David Sabatini (Addgene plasmid #1864 ...
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bioRxiv - Microbiology 2023Quote: ... The lentiviruses were also produced in HEK 293T cells by co-transfecting pLKO.1puro (Addgene #8453), psPAX2 (Addgene #12260) ...
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bioRxiv - Molecular Biology 2022Quote: Cell lines were engineered to express Cas9 by transduction with a lenti-Cas9-Blast (Addgene #52962) lentivirus and selection with 10 μg/mL blasticidin ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293T cells were transfected with viral packaging plasmids psPAX2 Addgene #12260) and pMD2.G (Addgene#12259), and pLKO.1 shRNA using Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: pPGK-Cre lentiviral backbone was amplified and transfected into 293T cells using VSV-G (Addgene 8454) and psPAX2 (Addgene 12260 ...
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bioRxiv - Cancer Biology 2023Quote: ... The constructs were PEI transfected into HEK293T cells along with psPAX2 lentiviral packaging construct (Addgene #12259) and pMD2.G envelope construct (Addgene #12259) ...
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The Hippo pathway terminal effector TAZ/WWTR1 mediates oxaliplatin sensitivity in colon cancer cellsbioRxiv - Cancer Biology 2023Quote: Murine Taz was expressed by transfecting cells with pEF-TAZ-N-Flag from Michael Yaffe (Addgene #19025 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were co-transfected with two gRNAs and pSpCas9n(BB)-2A-Puro (PX462) V2.0 (Addgene, 62987). After 24 h the cells were selected with puromycin and expanded ...
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bioRxiv - Genomics 2023Quote: ... HEK293T cells were co-transfected with two packaging plasmids (pCMV-VSV-G and delta8.9, Addgene #8454) and either a desired transfer plasmid ...
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bioRxiv - Genomics 2022Quote: ... HEK-293T cells were transfected with shRNA constructs and lentiviral packaging constructs pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260) ...
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bioRxiv - Cell Biology 2023Quote: We quantified the fluorescence of cells transduced with pFU-GW-sMYH6-mScarlet-TNNT2-mNeon (Addgene 170712) and ORF cDNA-expressing transcription factors on reprogramming day 6 ...
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bioRxiv - Cancer Biology 2022Quote: MMS22L-KO and AAVS1 control cells were infected with lentiviruses generated using LV-GFP (Addgene #25999) or LV-RFP (Addgene #26001 ...
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bioRxiv - Genetics 2023Quote: ... Muscle cells were then transfected with a pLenti-myc-GLUT4-mCherry (Addgene; Watertown, MA; stock # 64049) for 48 hours and the GLUT4 localization assay was performed as previously described(Lim ...
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bioRxiv - Developmental Biology 2023Quote: ... CRIPSRi activity in each cell line was validated by lentiviral transduction with pCRISPRia-v2 (Addgene #84832) backbones containing either a guide RNA (gRNA ...
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bioRxiv - Systems Biology 2022Quote: A549 cells were stably transduced with lentivirus from lentiCas9-Blast (Addgene #52962, gift from Feng Zhang) and subsequently selected using blasticidin ...
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bioRxiv - Cancer Biology 2023Quote: ... A673 cells were infected with pLV-EF1a-RNAseH1-IRES-Blast lentivirus (derived from Addgene plasmid #85133) or empty vector and selected for stably infected cells.
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bioRxiv - Cancer Biology 2023Quote: Lentivirus were generated by transfecting 293T cells with the indicated expression plasmid and the psPAX2 (Addgene) and pVSVG (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: SNAIL and SLUG knockout PANC-1 cell lines were generated using lentiCas9-Blast (Addgene plasmid 52962) and lentiGuide-Puro (Addgene plasmid 52963 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were co-transfected using polyethylenimine (PEI) with Cas9 (pU6-CBh-Cas9-T2A-BFP: Addgene 64323) and gRNA-Puro (pKLV2.2-h7SKgRNA-hU6gRNA-PGKpuroBFP ...
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bioRxiv - Molecular Biology 2023Quote: ... The stable cell line was then transduced with sgRNA-expression vectors LRG (Lenti_sgRNA_EFS_GFP; Addgene plasmid #65656) with gRNAs for IRF2BP2 ...