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Citations for Addgene :
8801 - 8850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... cre-dependent AAV9-hSyn-FLEX-GCaMP6s-WPRE-SV40 (∼2.0 x 1012 vg/ml; Addgene #100845) was injected unilateral into the VTA (Coordinates ...
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bioRxiv - Neuroscience 2023Quote: ... cre-dependent AAV9-hSyn-FLEX-GCaMP6s-WPRE-SV40 (∼2.0 x 1012 vg/ml; Addgene #100845) was bilaterally injected into the ARC (Coordinates ...
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bioRxiv - Neuroscience 2023Quote: ... Agrpcre/wt::Ghsrwt/wtand Agrpcre/wt::Ghsrlox/lox mice were injected with both cre-dependent AAV9-hSyn-FLEX-GCaMP6s-WPRE-SV40 (as above) and cre-dependent AAV5-hSyn-DIO-hM3D(Gq)-mCherry (∼2.0 x 1012 vg/ml; Addgene #44361) bilaterally injected into the ARC to test the functional capacity of AgRP GHSR KO to increase food intake to a ghrelin independent signal ...
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bioRxiv - Systems Biology 2023Quote: ... pHO_pTEFmut7_CggR_R250A_ble (#124585) and pCggRO-reporter (#124582) plasmids were purchased from Addgene (Monteiro et al., 2019). Then forward primer with BamHI cutting site and reverse primer with EcoRI cutting site were used to PCR CggRO sequence ...
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bioRxiv - Neuroscience 2023Quote: ... were secured in a stereotaxic frame and unilateral or bilateral injections of fluorogold or choleratoxin B subunit tracers dissolved in glycerol (FG 2% iontophoresis by 2 µA pulses with 2/2 s on/off duty cycle for 5 minutes and CTB 0.5% iontophoresis by 5 µA pulses with 2/2 s on/off duty cycle for 7-10 minutes) or retrograde pAAVrg-CAG-GFP (Addgene: 37825-AAVrg) or retrograde AAVrg-EF1a-mCherry-IRES-Flpo (Addgene ...
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bioRxiv - Neuroscience 2023Quote: ... CFP-GCaMP7s-tdTom - insert from GEFRI3 was cloned by BamHI/HindIII restriction into a pAAV CAG vector (Plasmid #100840 Addgene) by PCR amplification using the following primers ...
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bioRxiv - Neuroscience 2023Quote: ... AAV2/1-DIO-EF1α-mKate2-biPAC (Boston Children’s Hospital Vector Core; Addgene 169127) was unilaterally injected in the PVH of MC4R-2A-Cre mice (150 nl ...
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bioRxiv - Neuroscience 2023Quote: ... we used the pCMV(MinDis)-iGluSnFR (Plasmid #41732 Addgene) backbone ...
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bioRxiv - Neuroscience 2023Quote: ... AAV1-hSyn-FLEX-Chrimson-tdTomato (UNC Vector Core) and AAV5-hSynapsin1-FLEx-axon-GCaMP6s97 (Addgene 112010-AAV5) were injected bilaterally in the arcuate nucleus of AgRP-IRES-Cre and POMC-Cre mice ...
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bioRxiv - Neuroscience 2023Quote: ... AAV2/1-DIO-EF1α-mKate2-biPAC55 (Boston Children’s Hospital Vector Core; Addgene 169127) and AAV2/1-hSyn-DIO-GreenDownwardcADDis (Boston Children’s Hospital Vector Core ...
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bioRxiv - Neuroscience 2023Quote: ... We used sfGFP-N1 (Plasmid #54737 Addgene) as the backbone for inserting the stop codon with or without methionine following the N145 residue ...
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bioRxiv - Neuroscience 2023Quote: ... Mice were stereotaxically injected with 500nl of AAV.CamKII.GCaMP6f.WPRE.SV40 (Addgene # 100834) into the BLA (AP ...
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bioRxiv - Neuroscience 2023Quote: ... The synthetic gene was cloned into a pExp-DsbC expression vector (Addgene #129243) containing an N-terminal His 8-tag fused to a DsbC solubility enhancing tag ...
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bioRxiv - Neuroscience 2023Quote: The Pink Flamindo cDNA was amplified by PCR from the plasmid (provided by Kitaguchi, Addgene plasmid #102356). The amplified fragment was then integrated into the 20xUAS vector originating from pJFRC7-20xUAS-IVS-mCD8::GFP (Pfeiffer ...
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bioRxiv - Molecular Biology 2023Quote: ... template libraries were constructed by PCR sewing and cloned into pRSII413 (a gift from Steven Haase, Addgene plasmid #35450; http://n2t.net/addgene:35450; RRID:Addgene_35450) 89 by ligation (Figure S1A) ...
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bioRxiv - Molecular Biology 2023Quote: ... template libraries were constructed by PCR sewing and cloned into pRSII413 (a gift from Steven Haase, Addgene plasmid #35450 ...
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bioRxiv - Molecular Biology 2023Quote: FLAG-TAL1-short/long were cloned to MIGR1 vector (Addgene, plasmid#27490). HEK293T cells were co-transfected with the viral backbone vector pCMV-VSVG and pCL-Eco packaging vectors using polyethylenimine (PEI ...
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bioRxiv - Molecular Biology 2023Quote: shRNA sequence targeting TAL1 3’UTR (CATAACCACTGAAGGGAAT) was cloned to Tet-pLKO-puro vector (Addgene #8453). For lentivirus production ...
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bioRxiv - Neuroscience 2023Quote: Females were unilaterally injected with 300 nL of AA1-hSyn-Flex-GCaMP6s-WPre-SV40 (Addgene, #100845) in the aVMHvl (AP ...
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bioRxiv - Molecular Biology 2023Quote: pcDNA-dCas9-p300 Core plasmids (D1399Y; plasmid #61358 and plasmid #61357) were purchased from Addgene. pSPgRNA (Addgene ...
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bioRxiv - Molecular Biology 2023Quote: ... pSPgRNA (Addgene, plasmid #47108) was used as the gRNA plasmid ...
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bioRxiv - Neuroscience 2023Quote: ... AAV-rg/Cre (Addgene viral prep #24593-AAVrg) was used in PTENf/f;RosatdTomato mice and control RosatdTomato mice ...
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bioRxiv - Neuroscience 2023Quote: ... AAV-rg/Cre was used to delete PTEN controls received AAV-rg/GFP (Addgene viral prep #37825-AAVrg). In study 4 ...
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bioRxiv - Plant Biology 2023Quote: ... an expression clone was generated by cloning the tdT coding sequence (amplified from an AddGene-derived plasmid (Shaner et al., 2004)) into the BglII cloning site of plasmid pLAU2 using Gibson assembly (Idnurm et al. ...
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bioRxiv - Neuroscience 2023Quote: ... AAV5-Ef1a-DIO-EGFP (Addgene, 27056). AAV5-EF1a-DIO-eArch3.0-EYFP ...
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bioRxiv - Systems Biology 2023Quote: ... was designed pRDA_550 (Addgene #203398). Synthesis of DNA and custom cloning was performed by Genscript.
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bioRxiv - Systems Biology 2023Quote: ... we first swapped in puromycin resistance in place of blasticidin resistance from pRDA_174 (Addgene 136476). We then tested four locations for the insertion of a U6-guide expression cassette ...
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bioRxiv - Immunology 2023Quote: ... Vector pcDNA3.1-GFP (Addgene) was used as a template for the subcloning of GFP ...
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bioRxiv - Physiology 2023Quote: ... the CAG-pai expression cassette was inserted into the Pac I and Asc I sites of the pROSA26-PA backbone vector (Gift from Dr Frank Costantini, Addgene plasmid#21271 ...
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bioRxiv - Physiology 2023Quote: ... was replaced by the EF-1α promoter from the plasmid pEF.myc.ER-E2-Crimson (Gift from Dr Benjamin Glick; Addgene plasmid # 38770 ...
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bioRxiv - Immunology 2023Quote: ... Packaging plasmids pVSVG and pSPAX2 were purchased from Addgene. All constructs were cloned in the expression vector pLVX-Tight-Puro (Clontech Laboratories) ...
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bioRxiv - Cell Biology 2023Quote: GFP-PTEN was a gift from Alonzo Ross (Addgene plasmid # 13039 ...
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bioRxiv - Biochemistry 2023Quote: ... The pCMV-PE2 plasmid expressing PE2 Cas9-RT fusion protein (Addgene plasmid #132775 ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids used in this work included PKA biosensor pmAKAR3 (Allen and Zhang, 2006) from Jin Zhang (Johns Hopkins University pmCherry-FAK from Addgene (plasmid #35039).
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bioRxiv - Genetics 2023Quote: ... pZCS36 (Addgene ID 193048), pZCS38 (Addgene ID193049) ...
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bioRxiv - Genetics 2023Quote: ... the full resistance cassette was amplified from pCFJ910 (Addgene ID44481) and assembled into PCR linearized pUC19 vector to give pZCS38.
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bioRxiv - Genetics 2023Quote: ... from pCFJ1663 (Addgene ID51484). Overlapping PCR was used to fuse both HygR fragments ...
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bioRxiv - Genetics 2023Quote: ... pMS84 (Addgene ID 193852), pZCS36 (Addgene ID 193048) ...
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bioRxiv - Genetics 2023Quote: ... The germline licensed Cas9 and tbb-2 3’ UTR were amplified from pCFJ150-Cas9 (dpiRNA) (Addgene ID107940) (Zhang et al. ...
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bioRxiv - Cell Biology 2023Quote: ... For AHPH-GFP (Addgene; catalog no. 68026) active RhoA biosensor cells were fixed using 10% trichloroacetic acid (TCA ...
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bioRxiv - Biochemistry 2023Quote: ... as described in Janecek et al.39 Aurora B protein was expressed from plasmid pNIC28-AurB (Addgene 39119).
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bioRxiv - Cancer Biology 2023Quote: ... Didier Trono (Addgene plasmid #12260 and #12259; http://n2t.net/addgene:12260 and http://n2t.net/addgene:12259; RRID:Addgene_12260 and RRID:Addgene_12259). Polyjet (SignaGen SL100688 ...
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bioRxiv - Cancer Biology 2023Quote: ... Didier Trono (Addgene plasmid #12260 and #12259; http://n2t.net/addgene:12260 and http://n2t.net/addgene:12259; RRID:Addgene_12260 and RRID:Addgene_12259). Polyjet (SignaGen SL100688 ...
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bioRxiv - Cancer Biology 2023Quote: ... Daniele Gilkes (Addgene plasmid #141148 and #141147 ...
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bioRxiv - Developmental Biology 2023Quote: ... the pSpCas9 (BB)-2A-Puro (PX459) V2.0 (Addgene plasmid # 62988 ...
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bioRxiv - Developmental Biology 2023Quote: ... the pSpCas9 (BB)-2A-Puro (PX459) V2.0 (Addgene plasmid # 62988, Addgene, Teddington, UK) was modified by an EF1alpha promoter25 ...
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bioRxiv - Cancer Biology 2023Quote: ... Vectors used included pGIPZ-GFP-Puro (see Supplementary Materials) and psPAX.2 and pMD2.G (Addgene). Cell transfections were done in Opti-MEM (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... AAV pmSyn1-EBFP-Cre was a gift from Hongkui Zeng (Addgene plasmid # 51507; http://n2t.net/addgene:51507; RRID:Addgene_51507). Two copies of single miR-T sequences were added in primers ...
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bioRxiv - Neuroscience 2023Quote: ... Wilson (Addgene plasmid # 112865 ...
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bioRxiv - Neuroscience 2023Quote: ... Dre: AAV phSyn1(S)-DreO-bGHpA was a gift from Hongkui Zeng (Addgene plasmid # 50363 ...