Labshake search
Citations for Addgene :
8151 - 8200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... The Raf1 sequence was taken from the pDONR223-RAF1 construct ordered from Addgene, a gift from William Hahn & David Root (Addgene plasmid # 23832 ...
-
bioRxiv - Molecular Biology 2023Quote: ... or pCDNA3.3(+)ZKSCAN1 MCS Exon Vector (#69901, Addgene); both vectors can express circular RNAs of specific sequences in mammalian cells ...
-
bioRxiv - Microbiology 2023Quote: ... a gift from William Hahn & David Root (Addgene plasmid # 23832; http://n2t.net/addgene:23832; RRID:Addgene_23832). The FLAG tag was inserted with a Gibson assembly primer ...
-
bioRxiv - Neuroscience 2023Quote: ... were secured in a stereotaxic frame and unilateral or bilateral injections of fluorogold or choleratoxin B subunit tracers dissolved in glycerol (FG 2% iontophoresis by 2 µA pulses with 2/2 s on/off duty cycle for 5 minutes and CTB 0.5% iontophoresis by 5 µA pulses with 2/2 s on/off duty cycle for 7-10 minutes) or retrograde pAAVrg-CAG-GFP (Addgene: 37825-AAVrg) or retrograde AAVrg-EF1a-mCherry-IRES-Flpo (Addgene ...
-
bioRxiv - Neuroscience 2023Quote: ... CFP-GCaMP7s-tdTom - insert from GEFRI3 was cloned by BamHI/HindIII restriction into a pAAV CAG vector (Plasmid #100840 Addgene) by PCR amplification using the following primers ...
-
bioRxiv - Neuroscience 2023Quote: ... AAV2/1-DIO-EF1α-mKate2-biPAC (Boston Children’s Hospital Vector Core; Addgene 169127) was unilaterally injected in the PVH of MC4R-2A-Cre mice (150 nl ...
-
bioRxiv - Neuroscience 2023Quote: ... we used the pCMV(MinDis)-iGluSnFR (Plasmid #41732 Addgene) backbone ...
-
bioRxiv - Neuroscience 2023Quote: ... AAV1-hSyn-FLEX-Chrimson-tdTomato (UNC Vector Core) and AAV5-hSynapsin1-FLEx-axon-GCaMP6s97 (Addgene 112010-AAV5) were injected bilaterally in the arcuate nucleus of AgRP-IRES-Cre and POMC-Cre mice ...
-
bioRxiv - Neuroscience 2023Quote: ... AAV2/1-DIO-EF1α-mKate2-biPAC55 (Boston Children’s Hospital Vector Core; Addgene 169127) and AAV2/1-hSyn-DIO-GreenDownwardcADDis (Boston Children’s Hospital Vector Core ...
-
bioRxiv - Neuroscience 2023Quote: ... We used sfGFP-N1 (Plasmid #54737 Addgene) as the backbone for inserting the stop codon with or without methionine following the N145 residue ...
-
bioRxiv - Neuroscience 2023Quote: The backbone pSBbi-Pur (Plasmid #60523 Addgene) was amplified for inserting the restriction sites HindIII and XBaI using the following primers ...
-
bioRxiv - Neuroscience 2023Quote: ... 5’-AAACGGGATGCAGGGATGTCGACTc-3’) and cloning this into the unique BbsI sites of pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene # 42230), modified by the addition of a PGK-Puro cassette.
-
bioRxiv - Neuroscience 2023Quote: ... pAAV-CAG-eYFP (Plasmide #104055 Addgene), were digest with NheI and PstI ...
-
bioRxiv - Neuroscience 2023Quote: PA sfGFP-N1 (Plasmid #54736 Addgene) was used as the backbone to separate it the same way as mentioned above ...
-
bioRxiv - Neuroscience 2023Quote: ... pCDNA 3.1-GFP1-10 (Plasmid #70219 Addgene) was used as the backbone to which a stop codon and a partial Kozak sequence together with the 11th part of GFP were added by reverse PCR using the blunt primers ...
-
bioRxiv - Systems Biology 2023Quote: ... pHO_pTEFmut7_CggR_R250A_ble (#124585) and pCggRO-reporter (#124582) plasmids were purchased from Addgene (Monteiro et al., 2019). Then forward primer with BamHI cutting site and reverse primer with EcoRI cutting site were used to PCR CggRO sequence ...
-
bioRxiv - Microbiology 2023Quote: ... pLVX-EF1a-SARS-CoV-2-nsp16-IRES-puro was generated by subcloning the nsp16 coding sequence from pDONR223 SARS-CoV-2 NSP16 (Addgene #141269) into pLVX-EF1a-IRES-puro empty vector ...
-
bioRxiv - Microbiology 2023Quote: ... and pDONR223 SARS-CoV-2 spike (Addgene #149329) were subcloned into pLVpuro-CMV-N-3xFLAG (Addgene #123223 ...
-
bioRxiv - Microbiology 2023Quote: ... pLVX-EF1a-IRES-puro empty vector was generated by swapping the eGFP insert of pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro (Addgene #141395) with a multiple cloning site ...
-
bioRxiv - Microbiology 2023Quote: ... sequences were subcloned into pInducer20 (Addgene #44012) via Gateway cloning.
-
bioRxiv - Microbiology 2023Quote: 4e5 VeroE6 cells were plated in 6-well plates and transfected with 1 μg VSV-G plasmid (Addgene #8454) via TransIT-X2 (Mirus) ...
-
bioRxiv - Immunology 2023Quote: ... and inserted into a generic targeting vector (pBigT, Addgene, #21270), pBigT-mSparcl1 was then subsequently cloned into a plasmid with the ROSA26 genomic flanking arms (ROSA26-PA ...
-
bioRxiv - Neuroscience 2023Quote: ... the transgene p130PH or p130PHR134L were amplified using PCR from the pEGFP-N1 plasmids containing these genes31 and inserted into the Ef1α-DIO-mCherry viral vector (Addgene, #47636). AAVTM6 viruses were produced at Janelia Viral Tools facility ...
-
bioRxiv - Neuroscience 2023Quote: ... pAAV-EF1α-DIO-taCASP3-TEV was a gift from Nirao Shah (Addgene plasmid 45580) [65] ...
-
bioRxiv - Neuroscience 2023Quote: ... Roth (Addgene plasmids 44361 and 44362) [63] ...
-
bioRxiv - Neuroscience 2023Quote: ... was generated by inserting the GCaMP6s open reading frame from pGP-CMV-GCaMP6s (Addgene plasmid 40753, gift of Douglas Kim) [64] into the pAAV-hSyn-DIO-hM3Dq-mCherry backbone ...
-
bioRxiv - Neuroscience 2023Quote: ... and AAV8-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine (Addgene #50455) in the BLA (AP ...
-
bioRxiv - Neuroscience 2023Quote: ... Wilson (Addgene plasmid #105530; http://n2t.net/addgene:105530; RRID:Addgene_105530)
-
bioRxiv - Neuroscience 2023Quote: ... Mice were stereotaxically injected with 500nl of AAV.CamKII.GCaMP6f.WPRE.SV40 (Addgene # 100834) into the BLA (AP ...
-
bioRxiv - Immunology 2023Quote: CTLs were transiently transfected with the pEGFP plasmid encoding the EB1-GFP fusion protein (Addgene #17234, Watertown, Massachusetts, USA). Briefly ...
-
bioRxiv - Bioengineering 2023Quote: ... Pae Type I Cascade plasmids encoding Csy1-Csy2 (Addgene #153942) and Csy3-VPR-Csy4 (Addgene #153943 ...
-
bioRxiv - Bioengineering 2023Quote: ... MS2-modified gRNA expression plasmid (Addgene #61424), AsCas12a (Addgene #128136) ...
-
bioRxiv - Bioengineering 2023Quote: ... the scFv-GCN4-linker-VP16-GB1-Rex NLS sequence was PCR amplified from pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS (Addgene #60904) and cloned into a lentiviral backbone containing an EF1-alpha promoter ...
-
bioRxiv - Bioengineering 2023Quote: ... SpgRNA expression plasmid (Addgene #47108), MS2-modified gRNA expression plasmid (Addgene #61424) ...
-
bioRxiv - Bioengineering 2023Quote: ... GG-EBNA-OSK2M2L1-PP (Addgene #102898) and GG-EBNA-EEA-5guides-PGK-Puro (Addgene #102898 ...
-
bioRxiv - Bioengineering 2023Quote: ... Coli Type I Cascade system (Addgene #106270-106275) and Pae Type I Cascade System (Addgene #153942 and 153943) ...
-
bioRxiv - Bioengineering 2023Quote: ... SiT-Cas12a-Activ (Addgene #128136) was used ...
-
bioRxiv - Bioengineering 2023Quote: ... hSyn-AAV-EGFP (Addgene #50465) plasmid was used to generate different AAV based DNA constructs ...
-
bioRxiv - Bioengineering 2023Quote: ... CjCas9 was PCR-amplified from pAAV-EFS-CjCas9-eGFP-HIF1a (Addgene #137929) as two overlapping fragments using primers to create D8A and H559A mutations ...
-
bioRxiv - Bioengineering 2023Quote: ... 1x 9aa TADs were designed and annealed as double strand oligos and then cloned into the BamHI/NheI-digested MCP-p65-HSF1 backbone plasmid (Addgene #61423) using T4 ligase (NEB) ...
-
bioRxiv - Bioengineering 2023Quote: ... and Pae Type I Cascade System (Addgene #153942 and 153943), YAP-S5A (Addgene #33093 ...
-
bioRxiv - Bioengineering 2023Quote: ... Selected fusions between 3x 9aa TADs and eNRF2 were generated using gBlock (IDT) fragments and cloned into the BamHI/NheI-digested MCP-p65-HSF1 backbone plasmid (Addgene #61423) using NEBuilder HiFi DNA Assembly ...
-
bioRxiv - Bioengineering 2023Quote: ... the MCP-p65-HSF1 plasmid (Addgene #61423) was digested with BamHI and NheI and respective effector domains were cloned using NEBuilder HiFi DNA Assembly ...
-
bioRxiv - Bioengineering 2023Quote: ... AsCas12a (Addgene #128136), E ...
-
bioRxiv - Bioengineering 2023Quote: ... and GG-EBNA-EEA-5guides-PGK-Puro (Addgene #102898) used for reprogramming experiments have been described previously17 ...
-
bioRxiv - Bioengineering 2023Quote: ... and cloned into chimeric U6 promoter containing sgRNA cloning plasmid (Addgene #47108) and/or an MS2 loop containing plasmid backbone (Addgene #61424 ...
-
bioRxiv - Bioengineering 2023Quote: ... the dCas9-p300 plasmid (Addgene #83889) was digested with BamHI and then a synthetic double-stranded ultramer (IDT ...
-
bioRxiv - Bioengineering 2023Quote: ... were obtained from Addgene. The Csy3-VPR-Csy4 plasmid was digested with MluI (NEB ...
-
bioRxiv - Bioengineering 2023Quote: ... scFv-VP64 (Addgene #60904), SpgRNA expression plasmid (Addgene #47108) ...
-
bioRxiv - Bioengineering 2023Quote: ... The pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP (Addgene #60903) vector was used for dCas9-based scFv fusion protein recruitment to target loci ...