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Citations for Addgene :
8101 - 8150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... tFucci(CA)5 was a gift from Atsushi Miyawaki (Addgene plasmid # 153521).
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bioRxiv - Cancer Biology 2023Quote: pDONR223_CDKN1A_WT was a gift from Jesse Boehm & William Hahn & David Root (Addgene plasmid # 82201) was cloned into pInducer20 (a gift from Stephen Elledge ...
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bioRxiv - Cancer Biology 2023Quote: ... was cloned into pInducer20 (a gift from Stephen Elledge, Addgene plasmid # 44012) by using the Gateway LR Clonase II Enzyme Mix ...
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bioRxiv - Cancer Biology 2023Quote: ... These plasmids were gifts from Bernard Futscher (Addgene plasmid # 22945, # 22934 and # 22933). tFucci(CA)5 was a gift from Atsushi Miyawaki (Addgene plasmid # 153521).
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bioRxiv - Cancer Biology 2023Quote: ... 0.8 μg of pVSVG (Addgene, #36399) and 0.8 μg of the lentiviral plasmid hosting the cDNA of GFP lacking the first ATG (CTR ...
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bioRxiv - Cancer Biology 2023Quote: ... (ii) HEK293T with 1.3 μg of psPAX2 (Addgene, #12260), 0.8 μg of pVSVG (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... Paul Thomas (Addgene plasmid #100900, RRID:Addgene_100900). The FKBP-WRN donor plasmid construct and WRN targeting vector were verified using Sanger sequencing (ACGT DNA Sequencing Services).
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bioRxiv - Cancer Biology 2023Quote: Sequences for sgRNAs of interest were cloned into LRG2.1-GFP (Addgene #108098) (Tarumoto et al ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNAs were subcloned into the pMSCV-IRES-green fluorescence protein vector (Addgene #20672) with reference to a report by Masubuchi et al.43 The constructed plasmid was validated by sequencing prior to use.
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bioRxiv - Cancer Biology 2023Quote: CRISPR–Cas9 screen was performed using the whole genome human Brunello CRISPR knockout pooled library (Addgene #73178) (PMID ...
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bioRxiv - Cancer Biology 2023Quote: ... and pPAX2 1.5ug (Addgene, #12260), using 100uL of serum free DMEM media (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... pMDLg/pRRE 1.5ug (Addgene, #12251), and pMD2.G 0.5ug (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... the double-stranded oligonucleotide sgRNAs for human FOXM1 sequences were ligated into the BbsI sites of the FgH1tUTG plasmid (Addgene, #70183). sgRNA target sequences were listed in Supplementary Table 2 ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviruses and retroviruses were produced by co-transfecting the transfer plasmids with pMD2.G (Addgene #12259, a gift from Didier Trono) and psPAX2 (Addgene #12260 ...
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bioRxiv - Cancer Biology 2023Quote: ... MSCV MYC T58A puro (Addgene #18773, a gift from Scott Lowe)66 for expressing the mutant MYC ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfection of third generation lentiviral vectors included 2ug of lentiviral vectors FgH1tUTG and FUCas9Cherry (Addgene, #70182), packaging vectors pRSV-Rev 1ug (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviral cloning vector pLKO.1-TRC (Addgene, #10878) was used for shRNA expression ...
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bioRxiv - Cancer Biology 2023Quote: ... packaging vectors pMD2.G 0.5ug (Addgene, #12259) and pPAX2 1.5ug (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... Lenti-dCas9-KRAB-blast (Addgene #89567, a gift from Gary Hon)64 for constitutively expressing dCas9-KRAB fusion protein ...
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bioRxiv - Cancer Biology 2023Quote: ... and pMD2.G 0.5ug (Addgene, #12259) using 100uL of serum free DMEM media (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... packaging vectors pRSV-Rev 1ug (Addgene, #12253), pMDLg/pRRE 1.5ug (Addgene ...
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bioRxiv - Developmental Biology 2023Quote: ... TIR-1 was amplified from plasmid pLZ31 (Zhang et al., 2015) (Addgene #71720) and cloned under control of the hlh-3 promoter in pSL780 (Bone et al. ...
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bioRxiv - Biochemistry 2023Quote: ... pIRE4-Azi was a kind gift from Irene Coin (Addgene plasmid #105829; http://n2t.net/addgene:105829; RRID:Addgene_105829).
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bioRxiv - Biochemistry 2023Quote: ... pcDNA Myc DBC1 was a gift from Osamu Hiraike (Addgene plasmid # 35096 http://n2t.net/addgene:35096 ...
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bioRxiv - Biochemistry 2023Quote: ... and S545A (HS-BG) constructs were cloned into a pHIV-dTomato lentiviral backbone (Plasmid #21374, Addgene, Cambridge, MA) by the Center for Targeted Therapeutics Core Facility at the University of South Carolina (Columbia ...
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bioRxiv - Cell Biology 2023Quote: ... and pCe-BiFC-VC155 (Addgene) plasmids ...
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bioRxiv - Cell Biology 2023Quote: HeLa cells expressing DDX21-HA or OMP25-eGFP (expressed using pMXs-3XHA-EGFP-OMP25, Addgene: plasmid #83356) were cultured in 1x DMEM based media (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... OMM-jRCaMP1b (Plasmid #127871) and GFP-LC3 (Plasmid #22405) were purchased from Addgene, transfected to the cells for the indication of mitoCa2+ ...
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bioRxiv - Developmental Biology 2023Quote: ... Sense and antisense riboprobes were designed by subcloning fragments of coding cDNA sequences in pBlueScript II (SK or KS) plasmids (Addgene). Riboprobes were generated by in vitro transcription using the SP6/T7 DIG RNA labelling kit (Roche ...
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bioRxiv - Developmental Biology 2023Quote: Cre mRNA was created using pCAG-Cre plasmid (Addgene Catalog number #13775), mCherry mRNA was created using (Addgene pX330-U6-Chimeric_BB-CBh-hSpCas9 Cat# 42230 ...
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bioRxiv - Developmental Biology 2023Quote: ... pCAGEN-Ntn4 expression plasmid was constructed by cloning the full-length coding sequence out of mouse Ntn4-AP-His plasmid (Addgene 71980) using the primers in Table 1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Bot oligo – 5’-aaacTTGGCACTCCATTAGATCCG-3’) were cloned into U6.3>gRNA.f+e (#99139, Addgene) and electroporated at a concentration of 1.5 ug/ul ...
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bioRxiv - Developmental Biology 2023Quote: ... Cas9 mRNA was obtained from pCS2-Cas9 (Addgene #47322) as previously described52,53 ...
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bioRxiv - Developmental Biology 2023Quote: ... and accompanying Cas9-GFP (#99138, Addgene) at 2 ug/ul.
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bioRxiv - Developmental Biology 2023Quote: CRISPR constructs with gRNA non-targeting control (#99140, Addgene) or gRNAs targeting MOXD1 (MOXD1.1.gRNA Top oligo – 5’-cgtcCGGGCCGAACCTATCCGCAC-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... the primers 5’-AAACCTGGACCCCACCCCCAGATC-3’ and 5’-CACCGATCTGGGGGTGGGGTCCAG-3’ were annealed and cloned in the px458-pSpCas9(BB)-2A-GFP (Addgene, #48138) using the BpiI restriction site to generate a guide RNA ...
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bioRxiv - Cell Biology 2023Quote: ... They were inserted into the lentiCRISPRv2-puro vector (Addgene 52961) using the BsmBI restriction site ...
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bioRxiv - Cell Biology 2023Quote: ... sabaeus genome and cloned into pSpCas9(BB)-2A-Puro (a gift from Feng Zhang, Addgene # 62988) following the protocol in [68] ...
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bioRxiv - Developmental Biology 2023Quote: ... restriction site in the plasmid pU6-(BbsI)_CBh-Cas9-T2A-BFP (Addgene). The protocols for gRNA cloning and transformation of competent E ...
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bioRxiv - Developmental Biology 2023Quote: ... and GFPmRNA was made using pCAG-GFP (Addgene Cat# 11150).
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bioRxiv - Cell Biology 2023Quote: ... the cells were transfected with the SMO-expressing plasmid (pGEN_mSMO, Addgene #3767329) in OptiMEM medium using FuGENE® HD transfection reagent (Promega ...
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bioRxiv - Developmental Biology 2023Quote: The constructs for epigenome editing were based on the plasmid pSpCas9n(BB)-2A-GFP (Addgene #48140) (Ran et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... and 2 µg pMD2.G (#12259, Addgene) plasmids by using lipofectamine 2000 (Life Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... mito-mScarlet (vector-pmScarlet-i_C1, Addgene 85044), DENND6A-GFP (vector-pEGFP-N1) ...
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bioRxiv - Developmental Biology 2023Quote: We cloned a 691 bp fragment from the kni regulatory region (kni- RA) in pHP-Dest-eGFP (Plásmido #24566 Addgene) (Boy et al. ...
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Astroglia proliferate upon biogenesis of tunneling nanotubes and clearance of α-synuclein toxicitiesbioRxiv - Cell Biology 2023Quote: One population of U-87 MG cells were transiently transfected with pLV-mitoDsRed (Addgene #44386 ...
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bioRxiv - Cell Biology 2023Quote: ... except that: a) the gRNA Cloning Vector (Addgene, #41824, ref. 119) was used as template ...
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bioRxiv - Cancer Biology 2023Quote: ... The IRES site was amplified by PCR from pInducer-21 (Addgene #46948). YAP5SA was amplified by PCR from Addgene #33093 ...
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bioRxiv - Cancer Biology 2023Quote: ... pLV-mCherry was a gift from Pantelis Tsoulfas (Addgene plasmid #36084 ...
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bioRxiv - Cancer Biology 2023Quote: ... pLenti CMV Puro LUC (w168-1) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid #17477 ...