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Native Antigen
Cat# NAT41604-100,
100µg USD $426.0
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Scott P. Davies, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... subunit 1 (The Native Antigen Company), followed by Alexa Fluor 555-conjugated goat anti-rabbit IgG secondary antibody (Invitrogen ...
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No products found
because this supplier's products are not listed.
Lisa M. Russo, et al.,
bioRxiv - Microbiology 2021
Quote:
Streptococcus pneumoniae TIGR4 (serotype 4) was cultured statically in Todd Hewitt broth with 5% yeast extract (THY, BD Biosciences) supplemented with 300 U/ml catalase (Worthington Biochemicals ...
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No products found
because this supplier's products are not listed.
Sarhad Alnajjar, et al.,
bioRxiv - Microbiology 2020
Quote:
... IHC for Spn was performed using rabbit anti-Streptococcus pneumoniae polyclonal antibody (Thermo Fisher scientific cat. # PA-7259) followed by biotin-labeled goat anti-rabbit IgG antibody (Thermo Fisher Scientific Cat.# ...
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No products found
because this supplier's products are not listed.
Gareth B. Kitchen, et al.,
bioRxiv - Cell Biology 2020
Quote:
Streptococcus pneumoniae strain D39 (National collection of Type Cultures 7466) was prepared in Todd Hewitt Broth (Sigma) and incubated at 37°C 5% CO2 until an optical density of OD600 was achieved from 10ml of growth media ...
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No products found
because this supplier's products are not listed.
Bram Laukens, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... or Streptococcus pneumoniae β-N-Acetylhexosaminidase (Prozyme, 4 mU per digest) overnight at 37°C in 20 mM sodium acetate (pH 5.0).
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No products found
because this supplier's products are not listed.
Rebecca Kochanowsky, et al.,
bioRxiv - Microbiology 2022
Quote:
... Extracts (20µg) were separated on a 12% Tris-HCl native gel (BioRad, Hercules CA, USA). Purified bovine superoxide dismutase (10µg ...
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No products found
because this supplier's products are not listed.
Federica Dell’Annunziata, et al.,
bioRxiv - Microbiology 2021
Quote:
... pneumoniae ATCC 10031 was used for the OMVs purification. K. pneumoniae was transformed using the calcium chloride method with pGR (K. pneumoniae-pGR) (Addgene, Massachusetts, USA) and PRM-GFP (K ...
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No products found
because this supplier's products are not listed.
Cláudia Vilhena, et al.,
bioRxiv - Immunology 2022
Quote:
... pneumoniae antibody (Abcam®) was added ...
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No products found
because this supplier's products are not listed.
Beiyuan Liang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... After antigen retrieval using Antigen Unmasking Solution (Vector Laboratories), tissue slides were blocked with 10% Goat Serum (Vector Laboratories ...
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No products found
because this supplier's products are not listed.
M.E. McGrath, et al.,
bioRxiv - Microbiology 2022
Quote:
... Streptococcus pyogenes (New England Biolabs, Ipswich, MA), as described previously 13 An oligonucleotide containing 40 bases of homology to either side of the target accessory ORF was TAR-assembled with each appropriate Cas9-digested AP DNA fragment in yeast by spheroplast transformation ...
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No products found
because this supplier's products are not listed.
Zhuo Pan, et al.,
bioRxiv - Microbiology 2023
Quote:
... Native firefly luciferase (Promega) was diluted in holdase buffer (50 mM HEPES ...
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No products found
because this supplier's products are not listed.
Concha Ortiz-Cartagena, et al.,
bioRxiv - Microbiology 2022
Quote:
... pneumoniae ST846-OXA48 culture (Minutesiprep, Qiagen, Hilden, Germany). The khe and blaOXA-48 genes were consequently amplified from 5 µL of the extract by PrimeSTAR HS DNA polymerase ...
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No products found
because this supplier's products are not listed.
Timm Amendt, et al.,
bioRxiv - Immunology 2021
Quote:
Native human insulin (Merck) was pre-diluted in water to 1 mg/mL ...
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No products found
because this supplier's products are not listed.
Anastasia-Maria Zavitsanou, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... antigen retrieval was performed using antigen retrieval buffer pH=6 (Leica) for 20min ...
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No products found
because this supplier's products are not listed.
Magnus Bloch, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... for ‘native’ nanodiscs or synthetic POPC (Avanti Polar Lipids, Cat#850457) for ‘minimal’ nanodiscs ...
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No products found
because this supplier's products are not listed.
Pattama Wiriyasermkul, et al.,
bioRxiv - Biochemistry 2023
Quote:
... affinity columns of GST-fused Asct2(NT)-antigen and GST-fused Asct2(CT)-antigen were produced by conjugating the antigens with HiTrap NHS-activated HP (GE Healthcare). To purify anti-Asct2(NT ...
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No products found
because this supplier's products are not listed.
Kok-Siong Chen, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Antigen retrieval was performed using an antigen decloaking chamber (Biocare Medical, Pacheco, California). Mounted sections were heated to 125°C for 4 minutes at 15 psi in 10 mM sodium citrate buffer (pH 6.0 ...
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No products found
because this supplier's products are not listed.
Pan Liu, et al.,
bioRxiv - Biochemistry 2020
Quote:
... C4BP‐Ang1 or native Angpt1 protein (R&D systems) was mixed with 100 ng Tie2‐Fc (with His tag ...
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No products found
because this supplier's products are not listed.
Amanda P. Smith, et al.,
bioRxiv - Microbiology 2021
Quote:
... (ii) a rabbit polyclonal antibody to Streptococcus pneumoniae diluted 1:1000 (Cat# NB100-64502; Novus Biologicals, Littleton, CO), and (iii ...
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No products found
because this supplier's products are not listed.
Jessica Gray, et al.,
bioRxiv - Microbiology 2024
Quote:
... pneumoniae ECL8 electrocompetent cells were transformed with 0.2 µL of EZ-Tn5™ transposon (Epicentre) at 1.4 kV and recovered in brain heart infusion (BHI ...
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No products found
because this supplier's products are not listed.
Li-Feng-Rong Qi, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... Lysates were mixed with Native-PAGE sample buffer 5X (Beyotime) and subjected to Native-PAGE using ExpressPlus™ 4-12% PAGE Gel (GenScript) and Tris-MOPS-SDS Running Buffer (GenScript) ...
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No products found
because this supplier's products are not listed.
Neeltje van Doremalen, et al.,
bioRxiv - Microbiology 2023
Quote:
... spike S1 antigen (Sino Biological Inc. ...
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No products found
because this supplier's products are not listed.
Cole B. Matrishin, et al.,
bioRxiv - Microbiology 2022
Quote:
... yeast extract (VWR J850) - 5 g/L ...
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No products found
because this supplier's products are not listed.
Travis J Gates, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and underwent antigen retrieval in antigen retrieval buffer (AR9, PerkinElmer) in a 95LC water bath ...
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No products found
because this supplier's products are not listed.
Trixia M. Buscagan, et al.,
bioRxiv - Biochemistry 2022
Quote:
... native MoFe protein crystals (crushed using a seed bead Eppendorf tool with either a plastic bead or glass beads ...
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No products found
because this supplier's products are not listed.
Chandran Pfitzner, et al.,
bioRxiv - Genetics 2020
Quote:
... KAPA Express Extract kit (Roche), or MyTaq™ Extract-PCR Kit (Bioline) ...
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No products found
because this supplier's products are not listed.
Qidong Hu, et al.,
bioRxiv - Immunology 2022
Quote:
... Antibodies for extracellular antigens are CD3 (BioLegend 100220), CD4 (BioLegend 100540) ...
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No products found
because this supplier's products are not listed.
Carola Venturini, et al.,
bioRxiv - Microbiology 2019
Quote:
... pneumoniae isolates were sequenced by Illumina NextSeq (paired-end ...
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No products found
because this supplier's products are not listed.
Nancy G. Azizian, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Antigens were unmasked by boiling the slides for 15 min in antigen unmasking citrate buffer (Cell Signaling Technology 14746), and cooled at room temperature ...
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No products found
because this supplier's products are not listed.
Timothy Leach, et al.,
bioRxiv - Cell Biology 2022
Quote:
Native human lung fibroblasts (Lonza, Basel, Switzerland) were grown in Gibco™ Minimal Essential Media Alpha (Thermofisher ...
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No products found
because this supplier's products are not listed.
Shuxing Yu, et al.,
bioRxiv - Microbiology 2023
Quote:
... coli-Streptococcus shuttle vector pDL278 by using an In-Fusion HD cloning kit (TaKaRa, Japan) as described previously [49] ...
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No products found
because this supplier's products are not listed.
Kaijie Zheng, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Extract was sonicated (Diagenode Biorupter) at full power for 10 minutes at 4°C ...
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No products found
because this supplier's products are not listed.
Gaël Moquin-Beaudry, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... SV40 LargeT antigen (Santa Cruz, clone Pab 101), and GD2 (Biolegend ...
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No products found
because this supplier's products are not listed.
Silke Schmidt, et al.,
bioRxiv - Microbiology 2023
Quote:
... extracts were ultracentrifuged (Beckman Coulter Optima) at 100 000 rpm for two hours ...
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No products found
because this supplier's products are not listed.
Xiaoling Yu, et al.,
bioRxiv - Bioengineering 2019
Quote:
... pneumoniae isolates ATCC700603 (American Type Culture Collection, ATCC).
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No products found
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Jia Mun Chan, et al.,
bioRxiv - Microbiology 2023
Quote:
... pneumoniae and control reagents were resuspended or diluted in pre-warmed HEK-BlueTM Detection medium (Invivogen). 5 x 104 HEK-BlueTM hTLR2 cells were mixed with 5 x 105 CFU S ...
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No products found
because this supplier's products are not listed.
Sumit Bhattacharyya, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Nuclear extracts were prepared using a nuclear extract preparation kit (Active Motif) and were added to the wells of a 96-well microtiter plate ...
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No products found
because this supplier's products are not listed.
Elaine Thai, et al.,
bioRxiv - Immunology 2020
Quote:
Antigen ELISA detHigh binding 384 well polystyrene plates (Corning) were coated with recombinantly expressed PfCSP71-104 comprising N-CSP at 50 ng/well overnight at 4°C ...
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No products found
because this supplier's products are not listed.
Li Qin, et al.,
bioRxiv - Microbiology 2023
Quote:
... Protein extracts were incubated with GFP-Trap (ChromoTek) beads for 1h at 4°C ...
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No products found
because this supplier's products are not listed.
Alexandra R. Harris, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 50 μl of a Rat Tail Collagen I (Corning)/basement membrane extract (Trevigen) (0.18 mg/ml Collagen ...
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No products found
because this supplier's products are not listed.
Fatema-Zahra M. Rashid, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 0.5% yeast extract (Alfa Aesar), 80 mM NaCl ...
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No products found
because this supplier's products are not listed.
Katherine Minjee Chung, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... in a 2100 Antigen Retriever system (Electron Microscopy Sciences). Sections were then incubated with dual endogenous peroxidase block (Dako ...
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No products found
because this supplier's products are not listed.
Mathieu Pottier, et al.,
bioRxiv - Plant Biology 2020
Quote:
... purified using Nucleospin Extract II kit (Macherey-Nagel, Düren, Germany) and rigorously quantified through UV (260 nm ...
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No products found
because this supplier's products are not listed.
Joaquín Bayard-Bernal, et al.,
bioRxiv - Microbiology 2022
Quote:
... Teichoic acid polysaccharide (cell wall PS, CWPS) from Streptococcus pneumoniae strain CSR SCS2 was from Statens Serum Institut (Ref 3459).
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No products found
because this supplier's products are not listed.
Kim S. Friedmann, et al.,
bioRxiv - Immunology 2020
Quote:
... Afterwards antigen specific CD8+ T cells were isolated using IFN-γ secretion assay (Miltenyi Biotec). Single cells were seeded into individual wells (1 cell/200 μl in each well ...
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No products found
because this supplier's products are not listed.
Ruofan Wang, et al.,
bioRxiv - Microbiology 2020
Quote:
... 5 pmol Streptococcus Pyogenes NLS-Sp.Cas9-NLS (SpCas9) nuclease (Aldevron) was combined with 15 pmol total synthetic sgRNA (5 pmol each sgRNA ...
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No products found
because this supplier's products are not listed.
BL Spencer, et al.,
bioRxiv - Microbiology 2021
Quote:
... run on a native gel (12% Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad), and transferred to a membrane using BioRad’s Trans-Blot Turbo Transfer System (high molecular weight settings) ...
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No products found
because this supplier's products are not listed.
Brian L. Hie, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
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No products found
because this supplier's products are not listed.
Jun Feng, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... The crude DNA extracts were purified by Zymo DNA Clean & Concentrator™-5 kit (Zymo Research ...
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No products found
because this supplier's products are not listed.
Shiva Emami, Ameer Y. Taha,
bioRxiv - Biochemistry 2021
Quote:
... The extracts were transferred to LC vials (Phenomenex, CA) prior to UPLC-MS/MS analysis ...