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Citations for GE Life Sciences :
1 - 50 of 187 citations for Streptococcus Pneumoniae Antigen Native Extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... affinity columns of GST-fused Asct2(NT)-antigen and GST-fused Asct2(CT)-antigen were produced by conjugating the antigens with HiTrap NHS-activated HP (GE Healthcare). To purify anti-Asct2(NT ...
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bioRxiv - Cell Biology 2022Quote: ... with Amersham HMW native marker kit (17044501; GE Healthcare) as a molecular weight marker ...
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bioRxiv - Cell Biology 2024Quote: ... with Amersham HMW native marker kit (17044501; GE Healthcare) as a molecular weight marker ...
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bioRxiv - Genetics 2020Quote: ... using native Msh5 antibody and Protein A Sepharose beads (GE Healthcare). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... Native gels were imaged using Amersham Typhoon scanner (GE Healthcare Bio-Sciences AB), using Cy2 filter at a pixel size ...
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bioRxiv - Genetics 2024Quote: ... Native and denatured in-gel hybridization intensities were quantified using ImageQuant-TL (GE Healthcare Inc.).
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bioRxiv - Cancer Biology 2019Quote: ... on an EDB antigen-coated sensor chip (CM5, GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... whilst antigen-B was purified with rProteinA Sepharose (GE Healthcare). Affinity tags were cleaved by incubation with either thrombin (Merck ...
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bioRxiv - Genetics 2023Quote: ... and native and denatured in-gel hybridization intensity was quantified using ImageQuant-TL (GE Healthcare Inc.).
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bioRxiv - Immunology 2019Quote: ... These antigens were immobilised onto CM5 sensor chips (Biacore, GE Healthcare, Amersham) by amine coupling to a level of 2000 RU.
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bioRxiv - Biochemistry 2023Quote: ... Antigen B was further purified from Fc using rProteinA Sepharose (GE Healthcare), followed by deglycosylation with endoH (New England Biolabs) ...
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bioRxiv - Pathology 2019Quote: ... The antigen-antibody reaction was detected by ECL incubation (GE Healthcare Life Sciences) for 5 minutes on Licor C-DiGit Blot Scanner ...
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bioRxiv - Cancer Biology 2021Quote: ... Antigen revelation was performed using the ECL kit (GE Healthcare, Chicago, IL, USA) and images were acquired on a MyECL machine (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The antigen-antibody complexes were visualized using an enhanced chemiluminescence system (GE Healthcare) and captured by Azure 500 system (Azure Biosystems).
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bioRxiv - Microbiology 2020Quote: ... coli BL21(DE3) and were isolated under native purification conditions using a 5 HisTrap High Performance Column (GE Healthcare) with imidazole gradient (10-250 mM ...
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bioRxiv - Cell Biology 2021Quote: ... labelled probe was purified by native TBE-PAGE gel and illustra G-25 columns (GE Healthcare, Cat. No. 27532501). Equal intensity of different RNA probes were mixed with equal molar of different C/EBPα proteins individually in EMSA buffer B1 (Active Motif ...
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bioRxiv - Biochemistry 2024Quote: Native and selenomethionine-substituted BT4246 proteins were purified using a 5 ml Hi-Trap metal affinity cartridge (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: Protein extracts were precleaned with Protein G Sepharose (GE Healthcare) and subjected to immunoprecipitation with a combination of anti-FLAG M2 and Protein G Sepharose beads ...
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bioRxiv - Cell Biology 2020Quote: ... Crude extracts were incubated with Glutathione sepharose 4B (GE healthcare) resin for 3 hours at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... and the antiserum was affinity-purified on antigen-coupled CNBr-activated agarose (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... Each sample bound across the antigen surface was dissociated by HBS-EP (GE Healthcare) + running buffer for 300 s at a flow rate of 30 μL/min ...
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bioRxiv - Microbiology 2024Quote: The CP antigen was purified using a 5 mL HisTrap column (17524801, GE Healthcare) followed by a 5 mL HiTrap Q HP column (17115401 ...
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bioRxiv - Biochemistry 2024Quote: ... Antibody–antigen complexes were visualized using the ECL plus chemiluminescence detection system (GE Healthcare) using the Stella imaging system (Raytest ...
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bioRxiv - Biochemistry 2019Quote: ... Complexes were then run using 5% native PAGE in 0.5× TBE and visualized using the Typhoon 9400 variable mode laser scanner (GE Healthcare).
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bioRxiv - Physiology 2019Quote: ... Purity of the isoHb fractions was verified by means of native polyacrylamide gel electrophoresis (PAGE) using a PhastSystem (GE Healthcare) in 10-15% gradient precast gels ...
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bioRxiv - Biochemistry 2020Quote: The native molecular mass of BdiUSPPase and BdiGolSase1 were determined by using a Superdex 200 10/300 column (GE Healthcare), equilibrated with Buffer G ...
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bioRxiv - Biophysics 2021Quote: ... Reaction mixtures (18.5 μl) were resolved on 5% Tris-Glycine native gels and imaged on a Typhoon scanner (GE Healthcare).
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bioRxiv - Molecular Biology 2023Quote: ... The resulting mixtures were analyzed by 6% native TBE polyacrylamide gel electrophoresis and visualized using Typhoon Trio equipment (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: Protein molecular mass at the native state was determined by gel filtration using a Superdex200 10/300 column (GE Healthcare). A gel filtration calibration kit (high molecular weight ...
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bioRxiv - Molecular Biology 2023Quote: ... Then the samples were subjected to electrophoresis through 5% native polyacrylamide gel in 0.5x TBE buffer and imaged with 9410 Typhoon variable mode imager (GE Healthcare).
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bioRxiv - Developmental Biology 2019Quote: ... nuclear extracts were first fractionated on High Trap Q (GE Healthcare) prior to Flag-IP ...
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bioRxiv - Biochemistry 2019Quote: Cleared cell extracts were mixed with glutathione-Sepharose beads (GE Healthcare) conjugated to LC3 family proteins or GST ...
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bioRxiv - Genetics 2020Quote: ... Those extracts were cleared with protein G Sepharose beads (GE Healthcare) for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... were loaded with 10 μL of Aβ42 oligomer samples alongside the Amersham High Molecular Weight calibration kit for native electrophoresis (GE Healthcare, USA). The gels were run at 4 °C using the electrophoresis system according to the Invitrogen instructions (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The reaction mixture containing the protein–DNA complex were resolved on a 5% native PAGE gel on ice and transferred to a nylon membrane (Amersham Hybond-XL, GE healthcare) using 0.5 X TBE ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 (DE3) pLysS and purified under native conditions by ion exchange chromatography using Q-Sepharose Fast Flow (GE Healthcare, Sweden) as described previously [66] ...
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bioRxiv - Microbiology 2021Quote: ... full-length gB-Fab complexes were evaluated by Native PAGE and purified on a Superose-6 column (GE Healthcare Life Sciences) into TBS pH7.4 to remove aggregates.
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bioRxiv - Microbiology 2021Quote: The native molecular mass of the recombinant proteins was determined by gel filtration using a Superdex 200 10/300 column (GE Healthcare), previously calibrated with protein standards (GE Healthcare) ...
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bioRxiv - Molecular Biology 2019Quote: ... All samples were run in a 10% polyacrylamide native gel and then imaged using a Typhoon FLA9500 gel scanner (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... Eluted fractions were then exchanged into native binding buffer using a HiPrep Desalting 26/10 column on an ÄKTA pure FPLC (GE Healthcare). N-terminal tags were removed from Elp proteins using His-tagged tobacco etch virus (His-TEV ...
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bioRxiv - Microbiology 2023Quote: 4 mg of native protein standard was loaded to calibrate the gel filtration column (Superdex 200 increase 10/300 GL column, GE Healthcare). Following this ...
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bioRxiv - Biophysics 2023Quote: ... were loaded with 10 μL of Aβ42 oligomer samples alongside the Amersham High Molecular Weight calibration kit for native electrophoresis (GE Healthcare, USA). The gels were run at 4°C using the electrophoresis system according to the Invitrogen instructions (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... was synthesized by ABclonal Technology and got the 2 mg/mL purified antigen through Ni-IDA (GE Healthcare, USA). The mouse polyclonal antibodies against two parts of PGRP-S2 (NM_001163716.1) ...
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bioRxiv - Biochemistry 2023Quote: ... The antigen–antibody complexes were pulled down by 30 μl Protein A-Sepharose (GE Healthcare) and specific proteins were detected by the Western blotting analysis ...
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bioRxiv - Genetics 2020Quote: ... the protein extract was incubated with IgG Fast Flow Sepharose (GE Healthcare) and beads were washed with lysis buffer ...
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bioRxiv - Neuroscience 2021Quote: ... The crude extract was applied to 1ml Glutathione Sepharose beads (GE Healthcare) previously equilibrated in Buffer A (PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Clarified protein extracts were loaded onto a HisTrap HP column (GE Healthcare) pre-equilibrated in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2019Quote: ... Extracts were precleared for 1 h with protein A-Sepharose (GE Healthcare) supplemented with 1mg/ml BSA ...
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bioRxiv - Cell Biology 2020Quote: ... The extracts were then incubated with protein A-Sepharose beads (GE Healthcare) for 3 h at 4°C and washed five times with EB containing 1% Tween 20 ...
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bioRxiv - Synthetic Biology 2023Quote: ... the crude extract was desalted in Sephadex G-25 Columns (GE Healthcare) and reduced with 20 mM DTT in 50 mM Tris-HCl (pH 7.5 ...