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Citations for Promega :
1 - 50 of 174 citations for Streptococcus Pneumoniae Antigen Native Extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Native firefly luciferase (Promega) was diluted in holdase buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... native firefly luciferase (Promega) was denatured in 8 M guanidine hydrochloride for at least 1 hour at room temperature and then diluted into assay buffer (28 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... Native total calf liver tRNA (Promega) and in vitro transcribed tRNASer were aminoacylated using Escherichia coli methionyl tRNA synthetase (for obtaining Met-tRNAiMet ...
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bioRxiv - Molecular Biology 2023Quote: Native total calf liver tRNA (Promega) and in vitro transcribed tRNAiMet were aminoacylated using recombinant E ...
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bioRxiv - Microbiology 2023Quote: ... pneumoniae for up to six hours according to manufacturers’ instructions (ROS-GloTM H2O2 Assay, Promega). ROS-GloTM Detection solution was added to the cells and incubated for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... insert (NRP1, native human ORF in pF1K, FXC23812, Promega): 5’-atagggcgaattcggtagtaaggagcgatcgc-3’ (fwd ...
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bioRxiv - Molecular Biology 2020Quote: ... Native 80S ribosomes were purified from 200 μl RRL (Promega), which was layered onto 10-30% sucrose density gradients in buffer A (20 mM Tris [pH 7.5] ...
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bioRxiv - Biochemistry 2022Quote: ... Mass Spec-Compatible Human Protein Extracts, Cat# V6951) and digested yeast protein extract (Mass Spec-Compatible Yeast Protein Extracts, Cat# V7461) were from Promega, commercial human plasma samples (Human Source Plasma ...
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bioRxiv - Synthetic Biology 2022Quote: ... wheat germ extract (Promega), insect Spodoptera frugiperda Sf21 cell line extract (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... frugiperda cell-free extract (Promega) as described (Pisarev et al. ...
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bioRxiv - Synthetic Biology 2022Quote: ... and rabbit reticulocyte extract (Promega). All reactions were performed according to manufacturer’s instructions.
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m7G cap-eIF4E interaction stimulates polysome formation by enhancing first-round initiation kineticsbioRxiv - Biophysics 2021Quote: Ribosome depleted wheat germ extract (RdWGE) was generated by centrifugation of wheat germ extract (Promega) for two rounds ...
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bioRxiv - Plant Biology 2021Quote: ... and Wheat Germ Extract Systems (Promega, L4380), according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Extracts were treated with DNase I (Promega) and reverse transcribed using SuperScript III First-Strand Synthesis System (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... mRNAs were translated in wheatgerm extract (Promega) supplemented with 20 mM amino acids (lacking methionine ...
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bioRxiv - Cancer Biology 2022Quote: ... Biotinylated antigens were immobilized using streptavidin-coated magnetic beads (Promega). In each round ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... HaloTag-GST was used as a negative control antigen (Promega).
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bioRxiv - Neuroscience 2021Quote: ... Substrates, Coelenterazine native (CTZ, NanoLight technology cat. no. 303) and Furimazine (FMZ, Promega cat. no. N1110) were added using a micro-injector connected to the plate reader (BioTek Synergy HT) ...
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bioRxiv - Biochemistry 2021Quote: ... yeast (Saccharomyces cerevisiae) cell protein extract (Cat# V7341, V7461), and human K562 cell protein extract (Cat# V6951, V6941) were purchased from Promega Inc (WI ...
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bioRxiv - Immunology 2024Quote: ... Purified EDIII antigens were biotinylated using HaloTag PEG biotin ligand (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... coli S30 Extract System for Linear Templates (Promega). Reactions containing 200 ng of mRNA and 0.1 mM of D-luciferin were carried out in 5 μL aliquots at 37°C for 15 minutes and the activity of in vitro synthesized luciferase was measured by VictorX5 (PerkinElmer ...
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bioRxiv - Cell Biology 2022Quote: ... the extracts were incubated with DNAse buffer (Promega) and residual DNAse was subsequently inactivated with DNAse stop solution (Promega) ...
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bioRxiv - Genetics 2022Quote: ... Extracts were mixed with Luciferase Assay Reagent (Promega) in a 1:5 ratio and monitored for luminescence.
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bioRxiv - Bioengineering 2021Quote: ... coli S30 extract system for circular DNA (Promega) were supplemented with 2.5 μM EL222 ...
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bioRxiv - Bioengineering 2021Quote: ... coli S30 extract system for circular DNA (Promega) were supplemented with 2.5 μM of Gluc-EL222 ...
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bioRxiv - Bioengineering 2021Quote: ... coli S30 extract system for circular DNA (Promega).
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bioRxiv - Bioengineering 2021Quote: ... coli S30 extract system for circular DNA (Promega) for RFP expression were supplemented with 2.5 μM EL222 (unless stated otherwise ...
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bioRxiv - Microbiology 2022Quote: ... coli S30 Extract System for Linear Templates (Promega). The 50-µl reactions were composed of kit components ...
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bioRxiv - Molecular Biology 2022Quote: ... extract was equilibrated with Passive Lysis Buffer (Promega), and aliquot was mixed with 8 mM DTT and NuPAGE LDS Sample Buffer (Invitrogen) ...
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bioRxiv - Synthetic Biology 2022Quote: ... insect Spodoptera frugiperda Sf21 cell line extract (Promega), and rabbit reticulocyte extract (Promega) ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli S30 Extract System for Circular DNA (Promega). The second kit was only used for the T7 RNA polymerase circuit ...
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bioRxiv - Molecular Biology 2023Quote: ... coli S30 Extract System for Linear Templates (Promega), according to the manufacturer’s instructions for radiolabelled translation ...
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bioRxiv - Cell Biology 2022Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer with Azure Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer with Azure Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell extracts were prepared using Passive Lysis Buffer (Promega), and luciferase reaction was induced using the Nano-Glo Dual-Luciferase Reporter Assay System (Promega) ...
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bioRxiv - Plant Biology 2019Quote: ... Soluble cell extracts were added to MagneGST beads (Promega) and incubated and washed as described in Bartlet et al ...
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bioRxiv - Cancer Biology 2022Quote: ... cell extracts were made in reporter lysis buffer (Promega). Protein concentrations of the cell lysates were measured by Bradford assay reagent (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... extracts were used in the corresponding GloTM Assay (Promega) using the standard protocol where 50 uL of extract was added with 50 uL of GloTM Detection Reagent (Promega ...
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bioRxiv - Microbiology 2019Quote: ... coli S30 Extract system for Linear Templates kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... coli S30 Extract System for Circular DNA (Promega, #L1020) to each well with introduction of 100 ng of pCR XL TOPO-T7NLuc per reaction making use of the upstream vector ...
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bioRxiv - Microbiology 2024Quote: ... coli S30 Extract System for Linear Templates (Promega, USA). The reactions were carried out in 5 μL aliquots containing 2 μL of S30 Premix ...
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bioRxiv - Plant Biology 2021Quote: ... the full length of the native GmDGAT1A coding region was cloned into a pGEMR-T Easy vector (Promega, USA). Site-directed mutagenesis was performed by inverse PCR using PrimeSTAR GXL polymerase (Takara ...
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bioRxiv - Molecular Biology 2019Quote: ... coli S30 Extract System for Linear Templates Kit (Promega #L1030) as previously described (C ...
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bioRxiv - Biochemistry 2021Quote: ... In vitro translation assays with wheat germ extract (Promega®) were performed according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Protein extracts were digested with sequencing-grade trypsin (Promega®) as previously described (Chicher et al. ...
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bioRxiv - Cell Biology 2019Quote: ... The following primary antibodies against the antigens were used: Tuj-1 (1:300, Promega), Synaptophysin (1:400 ...
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bioRxiv - Plant Biology 2020Quote: ... Antigen on the membrane was visualized with horseradish peroxidase-coupled anti-rabbit IgG (Promega) for VHA-a1 and VHA-a3 ...
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bioRxiv - Immunology 2021Quote: ... RBD and NTD antigens were site-specifically biotinylated using Halotag PEG biotin ligand (Promega), following the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... Antigen retrieval was performed by incubation with 20 μg/mL proteinase K (Promega, USA) for 20 minutes ...