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Citations for Takara Bio :
1 - 46 of 46 citations for Streptococcus Pneumoniae Antigen Native Extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... coli-Streptococcus shuttle vector pDL278 by using an In-Fusion HD cloning kit (TaKaRa, Japan) as described previously [49] ...
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bioRxiv - Microbiology 2022Quote: ... pneumoniae K2 capsule cluster from ATCC 43816 was cloned in two separate sections using In-Fusion Snap Assembly (Takara Bio USA). The genes from wcuF through wzx were cloned into PCR-linearized pBBR1MCS3 (48) ...
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bioRxiv - Genomics 2024Quote: ... and titer quantified using p24 ELISA antigen assay (Takara). MOI=5 was used to transduce 1x1097 EndoC-βH3 cells in culture media without pen/strep and puromycin.
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bioRxiv - Neuroscience 2023Quote: ... Native tdTomato fluorescence destroyed by combined ISH protocol was recovered by rabbit anti-DsRed (1:1000, Takara Bio Clontech # 632496 ...
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bioRxiv - Plant Biology 2020Quote: ... GFP-TSN-interacting proteins and native TSN were detected by mouse α -GFP (monoclonal antibody JL-8; Clontech) and rabbit α-TSN antibodies at final dilutions of 1:1,000 and 1:5,000 ...
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bioRxiv - Physiology 2021Quote: ... and gel extracted (Clontech Nucleospin Extract II Kit Cat# 636972). The linearized and purified pMLM313 fragment was used as template for in vitro transcription of Cas9 mRNA with mMESSAGE mMACHINE T7 ULTRA kit (Life Technologies)16 ...
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bioRxiv - Plant Biology 2021Quote: ... The CaMV 35S promoter was then replaced by the HB40 native promoter (1,859 bp upstream translation start site) using In-Fusion (Takara) to generate the HB40:HB40-GFP construct ...
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bioRxiv - Biochemistry 2023Quote: ... EPHA2- FN2 and antigen-A were purified using His 60 Ni Superflow resin (Takara), whilst antigen-B was purified with rProteinA Sepharose (GE Healthcare) ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting amplicons were PCR-purified (NucleoSpin® Extract II, Clontech). To clone the pool of oligonucleotides in the lentiCRISPRv2 vector ...
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bioRxiv - Biochemistry 2019Quote: ... A native poly-histidine region within the Spir-KIND domain is sufficient for binding of these constructs to TALON® resin (Clontech).
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bioRxiv - Biochemistry 2022Quote: The expression plasmid for N-terminal 6×His-tagged mCherry-Nter was constructed by inserting the sequence encoding DmAgo2 Nter (residues 1–398) amplified from the native DmAgo2 sequence into pCold I (TAKARA Bio) using the InFusion HD cloning kit (Clontech) ...
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bioRxiv - Cell Biology 2020Quote: ... and then clarified extracts were incubated with TALON metal affinity resin (Clontech) for 3 hours at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Cell extract is filtered and incubated with washed TALON beads (Takara 635501) at 4C for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Native tdTomato fluorescence destroyed by combined ISH protocol was recovered by rabbit anti-DsRed (1:1000, Takara Bio Clontech # 632496, RRID: AB_10013483) antibody and switched to the green channel using an Alexa Fluor 488 secondary ...
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bioRxiv - Neuroscience 2021Quote: ... to extract RNA and prepare cDNA libraries using SMARTseq v4 kit (Clontech, # 634888) and sequenced using HiSeq 2500 in 100 pairbase length fragments for a minimum of 1 million reads per sample ...
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bioRxiv - Microbiology 2024Quote: RNA extract used for sequencing above was treated with RNase-Free DNase (Takara) to remove contaminated DNA ...
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bioRxiv - Plant Biology 2020Quote: ... a 4.0 kb fragment containing the ERF019 gene and its native promoter was amplified and inserted into pBI101 with BamHI and SacI by In-Fusion HD Cloning Kit (Takara Bio, Shiga, Japan). For awf2 ...
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bioRxiv - Microbiology 2020Quote: ... Viral titer was determined by the ELISA p24 antigen assay (Lenti-X p24 Rapid Titer Kit, TaKaRa). Monocytes were infected immediately after purification by adding HIV-1BaL virus to the culture at MOI between 3 and 5 based on the p24 titer ...
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bioRxiv - Immunology 2020Quote: Levels of p24 antigen in the purified SARS-CoV-2 pseudotype solution was measured by ELISA (Takara). Mouse sera were heat inactivated ...
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bioRxiv - Developmental Biology 2020Quote: ... tetracycline-controlled transactivator (tTA) and the SV40 large T antigen polyadenylation signal (Takara Bio, Mountain View, CA), into B6SJLF1 fertilized eggs ...
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bioRxiv - Microbiology 2022Quote: ... Viral titer was determined by the ELISA p24 antigen assay (Lenti-X p24 Rapid Titer Kit, TaKaRa). Infections of fully differentiated MDM were performed after adherent growth in the presence of M-CSF for seven days ...
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bioRxiv - Cell Biology 2022Quote: Protein extracts were analyzed by SDS-PAGE and immunoblotting using α-GFP (monoclonal, Takara), α-mCherry (polyclonal ...
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bioRxiv - Developmental Biology 2023Quote: ... The concentration of tissue extracts was quantified using a BCA Protein Assay Kit (Takara). Samples were electrophoresed on 10% TGX Stain-Free gel (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... and also remaining polymerase enzyme by use of Nucleospin® Extract II Kit (Clontech Laboratories) following Manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... and the Gal4 AD fused with SV40 large T-antigen (pGADT7-T) from the Matchmaker Gold Yeast Two-Hybrid System kit (Takara Bio) were used as positive control ...
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bioRxiv - Cell Biology 2022Quote: ... The extract was incubated for 1 h at 4 °C with Talon resin (Clontech, Mountain View, CA), loaded onto a column ...
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bioRxiv - Molecular Biology 2022Quote: ... The DNA band was cut out and purified using the Nucleospin® Extract II Kit (Clontech Laboratories) protocol ...
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bioRxiv - Microbiology 2024Quote: ... All RNA in the extract samples were converted into cDNA using the cDNA EcoDry Premix (Clontech, USA). Quantitative real-time PCR was performed using a CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: Total genomic DNA of HeLa cells was extracted after irradiation using Total genomic DNA extract kit (TAKARA, China). Human nuclear GAPDH gene (forward ...
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bioRxiv - Immunology 2020Quote: ... The clarified extract was loaded onto a column containing TALON Metal Affinity Resin (BD Clontech, Mountain View, CA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and PCR inhibitors were removed from the DNA extracts using CHROMA SPIN+TE-400 Columns (Takara Bio, Inc.) and Amicon Ultra-0.5 (100K ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... DNA was purified using the standard phenol-chloroform extract method and subjected to DNase I (Takara, 2270 B) treatment and reverse transcription for DRIPc-seq library construction ...
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bioRxiv - Microbiology 2020Quote: ... lysed by sonication and the recombinant His-tagged protein purified from cell-free extracts using IMAC (Talon resin, Clontech) as previously described19.
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bioRxiv - Neuroscience 2022Quote: ... coli cells induced with 100 mM IPTG and purified from bacterial extracts using TALON beads (Takara Bio, Catalog # 635502) according to the vendor’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... 5 μl of cell extract was used in a buffer containing final concentrations of 52 mM HEPES pH 7.4 (Takara), 35 mM KGlu (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μl of cell extract was used in a buffer containing final concentrations of 52 mM HEPES pH 7.4 (Takara), 35 mM KGlu (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant His-tagged protein was purified from cell-free extracts using immobilised metal affinity chromatography (IMAC using Talon resin; Clontech) as described previously (34) ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant His-tagged protein was purified from cell free extracts in a single step using immobilized affinity chromatography (IMAC) with Talon resin (ClonTech) and dialyzed overnight into 20 mM Tris-HCl ...
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bioRxiv - Systems Biology 2020Quote: ... proteins in whole cell extract and immunoprecipitated fractions were digested with 2 mg/mL proteinase K (Takara Bio Inc., Kusatsu, Japan) at 42°C for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... Normalization was made by measuring the β-Gal activity after 1-hour incubation of 7.5 µl of cellular extract with 50 µl of β-Gal substrate (β-Gal detection kit II, Clontech).
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bioRxiv - Genomics 2022Quote: ... colonies were picked into 96-well plates and a small proportion of cells from each colony were used for DNA extraction using Quick Extract solution (Epicenter) and direct PCR with Prime STAR® GXL DNA Polymerase (Clontech). PCR amplicons were sequenced by Sanger to verify the deletion (SFigure 17).
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bioRxiv - Cell Biology 2021Quote: ... the clarified supernatant from the crude extract was carefully collected and mixed with TALON Metal Affinity Resin (Takara Bio, Cat# 635502) for 1 hour to allow for the binding of His-tagged AlkB protein to resin ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was isolated from vehicle and Lantana camara leaf ethanolic extract-treated MDA-MB-231 cells using RNAiso Plus (Takara, Japan) as per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant His-tagged protein was purified from cell-free extracts using immobilized metal affinity chromatography (IMAC using Talon resin; Takara Bio). The buffer used during the purification process was 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... DNA extracts were sent to Resistomap Oy (Helsinki, Finland) for HT-qPCR analysis using a SmartChip Real-time PCR system (TaKaRa Bio, Japan). The target genes included 27 ARGs and 5 MGEs (Table S3 ...
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bioRxiv - Microbiology 2023Quote: ... DNA extracts were sent to Resistomap Oy (Helsinki, Finland) for HT-qPCR analysis using a SmartChip Real-Time PCR system (TaKaRa Bio, Shiga, Japan). 10 ng DNA of each of the extracted samples were shipped to Resistomap for analysis ...