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Citations for Qiagen :
1 - 50 of 218 citations for Streptococcus Pneumoniae Antigen Native Extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... pneumoniae ST846-OXA48 culture (Minutesiprep, Qiagen, Hilden, Germany). The khe and blaOXA-48 genes were consequently amplified from 5 µL of the extract by PrimeSTAR HS DNA polymerase ...
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bioRxiv - Microbiology 2021Quote: ... pneumoniae D39 WT and all FQ-resistant mutants using the DNeasy Blood & Tissue Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s recommendations for Gram-positive bacteria ...
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In-silico enrichment of bacterial plasmids by chromosomal depletion using nanopore adaptive samplingbioRxiv - Genomics 2022Quote: ... Klebsiella pneumoniae (GCF_025837075.1) and Enterobacter hormaechei (GCF_001729785.1) DNA was extracted using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... Protein was purified under native conditions according to the QIAexpressionist (Qiagen) protocol ...
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bioRxiv - Bioengineering 2022Quote: ... and native rat tissue samples were homogenized in lysis Buffer RLT (Qiagen). Total RNA was isolated using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Cell Biology 2019Quote: ... All proteins were purified under native conditions by using Ni-NTA sepharose (QIAGEN) according to the manual.
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bioRxiv - Plant Biology 2023Quote: ... (2020) and purified in its native state using Ni-NTA agarose beads (Qiagen) as previously described (Seung et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were purified under native conditions by affinity chromatography using Ni–NTA agarose (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: Native and ectopic thymuses were excised and RNA was isolated using the RNeasy kit (QIAGEN). RNA was quantified with the Nanodrop 2000c Spectrophotometer (Thermo Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... coli lysates under native conditions by immobilised metal affinity chromatography using Ni-NTA agarose (Qiagen) essentially as described 12 using 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... coli lysates under native conditions as described in the fifth edition of the QIAexpressionist (Qiagen). The lysates were loaded onto a 1 ml HisTrap HP column (GE healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... extracts were treated with RNAse A (Qiagen, 19101) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... was used to extract DNA in QIAcube (Qiagen) from 40 mg of faecal pellet from each mouse ...
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bioRxiv - Plant Biology 2019Quote: Sitting-drop vapour-diffusion experiments identified suitable crystallisation conditions for native protein from the PACT screen (QIAGEN), with optimisation identifying the best conditions for crystal formation as 6 mg/mL protein combined 1:1 with 8% PEG 1500 ...
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bioRxiv - Genomics 2022Quote: ... The extracts were purified using modified PB buffer (Qiagen), washed twice with PE buffer (Qiagen ...
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bioRxiv - Biophysics 2021Quote: ... RNA extracts were treated with RNase-free DNase (Qiagen). Reverse transcription was performed with MMLV-RT (Invitrogen− ...
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bioRxiv - Molecular Biology 2020Quote: Purified recombinant poly-histidine protein was obtained by FPLC under native conditions using a Ni-NTA column (Qiagen), and a Biologic DUO-FLOW BIO-RAD chromatography system (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... coli cells grown at 37°C by addition of 0.2 mM IPTG and native protein purified using Nickel affinity chromatography according to the manufacturer’s protocol (Qiagen). For N-terminal His-tag removal ...
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bioRxiv - Genomics 2019Quote: ... beads were washed with native lysis buffer three times followed by RNA extraction using RNeasy mini kit (Qiagen). 12.5μl RNA was used for cDNA synthesis with Advantage RT-PCR kit (Clontech) ...
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bioRxiv - Microbiology 2020Quote: ... His-SFP1 was purified using Ni-NTA affinity purification under native conditions using the standard manufacturer’s protocol (Qiagen). Recombinant His-SFP1 was used to immunize female New Zealand white rabbits (Covalab ...
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bioRxiv - Plant Biology 2019Quote: ... 500 ng of each native library were treated by sodium bisulfite using the EpiTect Bisulfite kit (Qiagen, Valencia, CA) and purified according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed and recombinant proteins were purified under native conditions using the Ni-NTA Fast Start Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... was PCR amplified from plasmid (pSH515) containing the native His4 gene promoter and purified by PCR cleanup kit (Qiagen). DNA was eluted from the spin column with 10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... the RNA extract was further treated with DNase I (Qiagen). Ribosomal depletion (Ribo-Zero Gold technology) ...
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bioRxiv - Microbiology 2020Quote: RNA extracts were treated with DNAse I at UTK (Qiagen) and further DNase treatment was performed at MRDNA (Shallowater ...
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bioRxiv - Genetics 2023Quote: ... were used to extract DNA on the QIAcube system (Qiagen) generally following manufacturer’s instructions with some adjustments (Supplementary Tables S8 – S10) ...
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bioRxiv - Cancer Biology 2023Quote: ... Extracts were then treated with RNase free DNase (Qiagen, #79254). qRT-PCR products were measured using Power SYBR Green RNA-to-Ct 1-step kit (Applied Biosystem ...
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bioRxiv - Biophysics 2023Quote: ... Cleared extract was incubated with Ni-NTA Agarose slurry (Qiagen) for 30 min at 4°C with agitation ...
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bioRxiv - Biochemistry 2023Quote: ... Cleared extract was loaded onto 1 ml NiNTA resin (Qiagen) preequilibrated with Nickel buffer (25 mM HEPES-NaOH pH 7.4 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the RNA extract was treated using DNase (Qiagen cat#79254) and was quantified using a spectrophotometer ...
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bioRxiv - Genomics 2023Quote: ... A second DNA library was produced using 2.5 µL native DNA from the same sample after whole genome amplification procedure following the REPLI-G® (Qiagen) manufacturer’s recommendation.
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bioRxiv - Molecular Biology 2021Quote: ... the cleared cell extract was incubated with Ni-NTA Agarose (Qiagen) or Glutathione Sepharose 4B (Cytiva ...
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bioRxiv - Cancer Biology 2019Quote: ... Denatured extracts were bound to magnetic Ni-NTA beads (Qiagen #36111), while other lysed extracts were bound to magnetic anti-HA beads ...
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bioRxiv - Genetics 2021Quote: Sae2C extract was incubated with 2mL NiNTA resin (Qiagen, Germantown, MD) in batch ...
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bioRxiv - Genomics 2019Quote: ... was used to extract DNA with the automated QIAcube platform (Qiagen), following the DNeasy PowerWater IRT protocol ...
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bioRxiv - Genetics 2019Quote: ... expand them and extract genomic DNA (Puregene Core Kit B, Qiagen) to improve the quality of the sequencing reaction ...
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bioRxiv - Cancer Biology 2020Quote: mRNA prepared from cell extracts using the RNeasy Mini Kit (QIAGEN) was reverse-transcribed into cDNA using the qScript cDNA synthesis kit (Quanta Biosciences) ...
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bioRxiv - Systems Biology 2022Quote: The crude extracts were cleaned using the RNeasy Mini kit (Qiagen) with on column DNase I treatment ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The extract was purified using a MinElute PCR purification kit (QIAGEN) according to standard instructions and finally eluted in 110 μl of EB buffer.
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bioRxiv - Microbiology 2023Quote: ... the RNA extracts were treated with the DNase Max Kit (QIAGEN), following the manufacturer’s protocol ...
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... Extracts were incubated with 400 µl of Ni-NTA beads (Qiagen) for one hour at RT and then transferred to disposable columns and washed with 5 column volumes of extraction buffer containing 5 mM imidazole ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... DNA extracts were prepared using a DNA purification kit (Qiagen, 51106) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... RNA extracts were purified using the RNeasy Mini Kit (Qiagen #74104) with DNase I treatment as described in the manufacturer’s protocol.
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bioRxiv - Microbiology 2019Quote: ... Short CAP was expressed and His tag purified using Ni-NTA affinity purification under native conditions using the standard manufacturer’s protocol (Qiagen, Hilden, Germany). The shortCAP recombinant protein was used to immunize female New Zealand white rabbits (Covalab ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was purified from soluble fraction under native conditions using Ni2+– NTA affinity chromatography (Ni2+ beads obtained from Qiagen, #30210), followed by a second step of purification involving gel filtration chromatography using Superdex 200 10/300 GL (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... was used to extract RNA and the OneStep RT-PCR Kit (Qiagen) was used to synthesize cDNA ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was isolated from tissue extracts using RNeasy Mini Kit (QIAGEN, UK). The RNA concentration was measured using Qubit RNA HS Assay Kit (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... The cells extracts were then mechanically lysed in a TissueLyser LT (Qiagen), 5 × 1 minute at maximal speed with 1 minute on ice in between ...
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bioRxiv - Genetics 2021Quote: RNA extracts were obtained from cells using RNasey Mini kit (QIAGEN, 74104) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... The cell-free extract was bound to pre-equilibrated Ni2+-NTA (Qiagen) matrix for 3 h ...