Labshake search
Citations for New England Biolabs :
1 - 50 of 147 citations for Streptococcus Pneumoniae Antigen Native Extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... Streptococcus pyogenes (New England Biolabs, Ipswich, MA), as described previously 13 An oligonucleotide containing 40 bases of homology to either side of the target accessory ORF was TAR-assembled with each appropriate Cas9-digested AP DNA fragment in yeast by spheroplast transformation ...
-
bioRxiv - Biochemistry 2021Quote: ... in its native reaction buffer (NEB) by incubating at 72°C for 10 minutes ...
-
bioRxiv - Genetics 2020Quote: ... 1µl of 6000nM Streptococcus pyogenes Cas9 Nuclease (New England Biolabs, cat no. M0386; 200nM final), and pre-incubate this for 10 min ...
-
bioRxiv - Microbiology 2022Quote: ... pneumoniae D39 chromosomal DNA by PCR using Q5 High-Fidelity DNA polymerase (New England Biolabs). The kanamycin cassette was amplified from pSt-K and the streptomycin cassette from pGMC5-SM-RFP-PFurA-GFP-streptomycin ...
-
bioRxiv - Microbiology 2023Quote: ... pneumoniae SGH10 genomic DNAas template and the Q5 high-fidelity DNA polymerase (New England Biolabs). Then ...
-
bioRxiv - Microbiology 2023Quote: ... the native barcodes were ligated with the Native Barcoding Expansion 96 (EXP-NBD196) and NEB Blunt/TA Ligase Master Mix (NEB, M0367) selecting a unique barcode per sample ...
-
bioRxiv - Plant Biology 2023Quote: ... with the native ACO2 promoter using Gibson Assembly® (NEB) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... Glycans were incubated with different exoglycosidases in different sequences: (i) Streptococcus pneumonia β-N-acetylglucosaminidase (GUH, New England Biolabs); (ii ...
-
bioRxiv - Microbiology 2022Quote: ... 0.75 µl End-prepped DNA, 1 µl Native Barcode (Native Barcoding Expansion 96, EXP-NBD196) and 5 µl Blunt/TA Ligase Master Mix (NEB Blunt/TA Ligase Master Mix, M0367) were combined in a new reaction vessel and incubated for 20 minutes at room temperature ...
-
bioRxiv - Plant Biology 2020Quote: ... The powder was dissolved in a cold native extraction buffer (NEB: Tris HCl 100 mM pH 7.4 ...
-
bioRxiv - Bioengineering 2019Quote: ... pneumoniae chromosomes are incubated overnight at 37°C in presence of 1 µL of Cas9 Nuclease from Streptococcus pyogenes (M0386T, NEB) and 21 µg of gRNA transcripts produced by in vitro transcription of oligonucleotides (HiScribeTM T7 High Yield RNA Synthesis kit from NEB) ...
-
bioRxiv - Cell Biology 2021Quote: ... Single guide RNAs (sgRNAs) were synthesized using the EnGen sgRNA Synthesis Kit (for the Streptococcus pyogenes-originated Cas9, New England Biolabs). The SpCas9-sgRNA ribonucleoprotein (RNP ...
-
bioRxiv - Molecular Biology 2021Quote: ... repaired and end-prepped DNA was barcoded with Native Barcode by NEB Blunt/TA Ligase Master Mix (NEB) ...
-
bioRxiv - Physiology 2021Quote: ... Enzymatic antigen retrieval with proteinase K (Biolabs, 2μg/mL) was used prior to incubation with antibodies ...
-
bioRxiv - Biophysics 2023Quote: ... Chimeric NICDs were created from native NICDs using HiFi DNA Assembly (New England Biolabs). Boundaries for chimeric constructs are given in Table S3.
-
bioRxiv - Genomics 2019Quote: ... Native barcode ligation was performed with NEB blunt/ TA ligase (New England Biolabs, MA, USA) and cleaned with 0.5x AMPure XP beads ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... the native barcodes were attached using a NEBNext® Ultra™ II Ligation Module (NEB). Up to 23 samples were pooled for sequencing in the same flow cell (FLOMIN106 flow cell R9.4.1) ...
-
bioRxiv - Microbiology 2023Quote: ... Native barcode adapters NBD04 were ligated in Blunt/TA Ligase Master Mix (M0367S, NEB, USA), and resulting product was AMPure XP beads purified before pooling to produce a 54 μl equimass pool ...
-
bioRxiv - Microbiology 2023Quote: ... 2.5uL from a single native barcode and 25 µL Blunt/TA Ligase Mix (NEB, M0367S) were added ...
-
bioRxiv - Neuroscience 2023Quote: ... extracts were treated with DNaseI (NEB, M0303S) for 15 minutes at room temperature ...
-
bioRxiv - Genomics 2019Quote: ... Native barcodes were added by ligation using the Blunt/TA ligase master mix (New England Biolabs) and the ligation reaction was purified using 1 volume ratio of AMPure beads ...
-
bioRxiv - Biochemistry 2021Quote: ... The reactions were quenched with native loading dye containing EDTA (New England Biolabs, Cat. no. #B7025S). Samples were loaded on a 0.7% agarose gel and run at 120 V in 45□mM (0.5× ...
-
bioRxiv - Biochemistry 2021Quote: Native glycoproteins (10 μg) were digested with 1 U of IMPa (O-Glycoprotease, New England Biolabs) in 50 μL 20 mM Tris-HCl ...
-
bioRxiv - Microbiology 2023Quote: ... and the fleQ gene and its native promoter were amplified using Phusion polymerase (New England BioLabs) with primers containing 20bp of homology to the mTn7/pMQ56 SmaI cut-site at their 5’ end ...
-
bioRxiv - Microbiology 2024Quote: ... Barcodes (Native Barcoding Kit 96 (SQK-NBD109.96)) were ligated using Blunt/TA Ligase Master Mil (NEB). Barcoded libraries were purified using KAPA Pure Beads (Roche ...
-
bioRxiv - Biochemistry 2020Quote: ... was incubated for 30 minutes shaking at 1.500 rpm at room temperature with protein extract (500 µg of whole cell protein extract or 150 µg of CPE) and murine RNase Inhibitor (NEB) in the corresponding protein extraction buffer (without glycerol) ...
-
bioRxiv - Cancer Biology 2024Quote: ... genomic DNA was extracted in native conditions and digested by restriction enzyme cocktail fragmentation (BsrGI-HF NEB cat ...
-
bioRxiv - Biophysics 2024Quote: ... 10 µl of each sample was mixed with 4 µL native loading dye (1% Orange G (NEB) dissolved in 50% glycerol and 50% EMSA buffer ...
-
bioRxiv - Synthetic Biology 2024Quote: ... native adapter ligation and cleaning steps were performed using NEBNext Quick Ligation Module (New England Biolabs, USA) kit and Agencourt AMPure XP beads (Beckman Coulter ...
-
bioRxiv - Molecular Biology 2024Quote: ... Ligation of the Native barcode was then performed by adding Blunt/TA Ligase Master Mix (NEB M0367) and a unique native barcode (Oxford Nanopore Technologies EXP-NBD196 ...
-
bioRxiv - Pathology 2020Quote: ... Heat mediated antigen retrieval pretreatment using 10mM Sodium Citrate buffer (Vectors Biolabs) was used ...
-
bioRxiv - Molecular Biology 2022Quote: Cell extracts were prepared in RIPA lysis buffer (NEB) supplemented with protease inhibitors (Sigma ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cell extracts were batch bound to amylose resin (NEB) for 2 hours to capture the 2XMBP tagged BRCA2 proteins ...
-
bioRxiv - Genetics 2023Quote: ... RNA extracts were treated with DNAse (New England Biolabs) to ensure no genomic DNA contamination ...
-
bioRxiv - Biochemistry 2023Quote: ... ECE cell extract was supplemented with micrococcal nuclease (NEB) at a final concentration of 15 u/ml and 0.75 mM of calcium chloride (Sigma) ...
-
bioRxiv - Microbiology 2023Quote: ... RNA extracts were treated with DNAse (New England Biolabs) to ensure no genomic DNA contamination ...
-
bioRxiv - Genomics 2020Quote: ... instead of SuperScript IV (Thermo) and half volume native barcoding reactions using Blunt/TA Ligase Master Mix (NEB) instead of NEBNext Ultra II Ligation Module (NEB).
-
bioRxiv - Molecular Biology 2022Quote: ... Approximately 3-5μg of native genomic DNA were dephosphorylated with 0.05U/μl of shrimp alkaline phosphatase (rSAP) (New England BioLabs) to reduce the amount of off-target molecules in the final SMRTbell® library ...
-
bioRxiv - Microbiology 2024Quote: ... 1.25 µL native ligation barcode (ONT SQK-NBD114-96) and 3 µL Blunt/TA Ligase Master Mix (NEB). The reaction was mixed by pipetting ...
-
bioRxiv - Plant Biology 2023Quote: ... The genomic DNA was barcoded, adding Native Barcoding Expansion (ONT, Oxford, UK) and Blunt/TA ligase mix (NEB) was added for 10 minutes at room temperature and purified with 1X AMPure XP beads (Beckman Coulter) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Heat mediated antigen retrieval pretreatment using 10 mM Sodium Citrate buffer (Vectors Biolabs) were used ...
-
bioRxiv - Biochemistry 2021Quote: 10 ug of protein extract were digested with PNGaseF (NEB) according to manufacturer instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cell extracts (average OD 7) were treated with MNase (NEB), CaCl2 (5mM final concentration) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... For each extract non USER-treated and USER-treated (NEB) libraries were built 54 ...
-
bioRxiv - Molecular Biology 2021Quote: ... 22.5 µl of cDNA was combined with 2.5 µl native barcode and 25 µl Blunt/TA Ligase Master Mix (New England Biolabs). Samples were incubated at room temperature for 10 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... downstream of and in-frame with the 3X FLAG tag using the native restriction sites AgeI and NheI (NEB). Expression of both FLAG-tagged AaDNMT2 in mosquito cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich ...
-
bioRxiv - Microbiology 2021Quote: ... downstream of and in-frame with the 3X FLAG tag using the native restriction sites AgeI and NheI (NEB). Expression of both FLAG-tagged AaDNMT2 in mosquito cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich ...
-
bioRxiv - Microbiology 2021Quote: ... 22.5 µL of this was taken forward for native barcoding with the addition of 2.5 µL barcode and 25 µL Blunt/TA Ligase Master Mix (NEB) (final volume 50 µL) ...
-
bioRxiv - Microbiology 2021Quote: ... End-prepared DNA was ligated with native barcode adapters NBD04 using Blunt/TA Ligase Master Mix (M0367S, NEB, USA). Following the barcode ligation reaction ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Native PCC 11901 genetic parts were amplified from genomic DNA using Q5 High-Fidelity DNA Polymerase (New England Biolabs). Where necessary ...