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Citations for Macherey-Nagel GmbH :
1 - 32 of 32 citations for Streptococcus Pneumoniae Antigen Native Extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... purified using Nucleospin Extract II kit (Macherey-Nagel, Düren, Germany) and rigorously quantified through UV (260 nm ...
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bioRxiv - Biochemistry 2020Quote: ... Cleared extract was batch incubated with Ni-NTA beads (Macherey-Nagel) for 1 hour on a rotating wheel at room temperature for His-tag affinity purification ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid DNA was isolated using the Nucleospin Extract Kit (Macherey-Nagel), Gene Jet Mini Prep Kit (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: The protein was purified under native conditions using the Protino Ni-NTA Agarose for His-tag protein purification Kit (Macherey-Nagel) with the gravity flow method ...
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bioRxiv - Cell Biology 2022Quote: Total RNA extracts were obtained using the Nucleospin RNA kit (Macherey-Nagel) and cDNAs were synthetized using SuperScript III reverse transcriptase (Thermofisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... total RNA extracts were obtained using the Nucleospin RNA kit (Macherey-Nagel) and cDNAs were synthesized using SuperScript III reverse transcriptase (Thermofisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... DNA purification used the Nucleospin® extract II kit (Macherey-Nagel®), according to manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the Nucleospin® extract II kit (Macherey-Nagel) according to manufacturer’s instructions and were Sanger sequenced (Eurofins UK).
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the Nucleospin® extract II kit (Macherey-Nagel) according to manufacturer’s instructions and were Sanger sequenced (Eurofins UK).
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bioRxiv - Molecular Biology 2019Quote: ... total RNA was extracted using the Nucleospin RNA II extract kit (Macherey-Nagel, Germany) following the manufacturer instructions and full genome sequencing was attempted ...
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bioRxiv - Synthetic Biology 2020Quote: ... When required DNA was purified using the NucleoSpin Extract II (Macherey-Nagel, Düren, Germany). The oligonucleotides used in this work are indicated in Supplementary Table S2 in the Supplementary Material ...
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bioRxiv - Cell Biology 2019Quote: ... The soluble extract was incubated with 0.2 mL of Ni2+-NTA-agarose slurry (Macherey-Nagel) per 50 mL of starting culture ...
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bioRxiv - Synthetic Biology 2019Quote: The amplified DNA fragments were then gel purified with NucleoSpin Extract II kit (Macherey-Nagel) and quantified ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products of Acc1 were column-purified with the NucleoSpin Extract ΙΙ Kit (Macherey-Nagel).
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bioRxiv - Microbiology 2022Quote: ... a bulk (∼ 300,000 Euplotes cells) extract with the NucleoSpin™ Tissue Kit (Macherey-Nagel™) was processed through a SQK-LSK109 ligation-sequencing library ...
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bioRxiv - Molecular Biology 2022Quote: ... total RNA was isolated from extracts obtained from different fractions using NucleoSpin TriPrep Kit (Macherey-Nagel) following the requirements of the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... GST-tagged proteins in soluble extracts were purified by GSH-agarose beads (Macherey-Nagel, Düren, Germany) from which were eluted by the addition of 50 mM reduced L-glutathione (dissolved in 25 mM Tris-HCl pH 8).
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then purified from bacterial extracts by affinity chromatography using glutathione-conjugated agarose (Macherey-Nagel), followed by cleavage of the GST-tag with PreScission protease ...
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bioRxiv - Cancer Biology 2023Quote: ... The pooled extracts were further purified on a Chromabond C18 ec polypropylene column (Macherey-Nagel, Dueren, Germany). The extracted glycolipids were digested with LudgerZyme Ceramide Glycanase (CGase ...
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bioRxiv - Plant Biology 2021Quote: ... The organic extract was passed over a Chromabond NH2 500 mg solid phase extraction column (Macherey-Nagel, Düren, Germany). The eluate was diluted with distilled water and passed over a Chromabond C18ec 100 mg solid phase extraction column (Macherey-Nagel) ...
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bioRxiv - Molecular Biology 2019Quote: ... Then total RNAs were extracted from 100 µL of homogenates using the Nucleospin RNA II extract kit (Macherey-Nagel, Germany) and 200 µL were conserved at −80°C for attempts to isolate the virus ...
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bioRxiv - Cell Biology 2023Quote: ... The pooled extracts were further desalted and purified on a Chromabond® C18 ec poplyprolyene column (Macherey-Nagel, Düren, Germany). The extracted glycolipids were evaporated to dryness under nitrogen gas to remove organic solvents for deglycosylation ...
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bioRxiv - Molecular Biology 2020Quote: ... An amount of 0.25 g (3.6 g TSS L−1) activated sludge was processed to extract intracellular DNA using a NucleoSpin® Soil kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: The recombinant His-tagged AtDAT1 protein variants from this crude extract were purified with Protino Ni-NTA agarose from Macherey-Nagel, (Weilmünster Germany ...
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bioRxiv - Microbiology 2021Quote: ... The products were isolated by cutting the band from an agarose gel and clean-up with the NucleoSpin Extract II kit (Macherey-Nagel). Labelled DNA was generated using the same PCR program with the DIG Easy Hyb (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... DNA fragments were purified by agarose gel electrophoresis followed by extraction from an excised gel slice using a NucleoSpin Extract II kit (Macherey-Nagel). Hybridization probes were labeled with α[32P]dCTP by random priming (Multiprime DNA labeling kit ...
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bioRxiv - Plant Biology 2022Quote: ... DNA was extracted from the distant rhizosphere using a modified Griffiths’ protocole that co-extracts DNA and RNA (as described above) and from the root and close rhizosphere using NucleoSpin Plant II kit (Macherey-Nagel). DNA was sent for 16S rRNA and ITS sequencing on an Illumina MiSeq in pair-ended mode (2×250 ...
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bioRxiv - Cell Biology 2020Quote: ... Purification of respective proteins from bacterial extracts was performed by affinity chromatography using Protino glutathione-conjugated agarose 4B (Macherey-Nagel, Düren, Germany). Except for GST-WAVE2-WCA ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... The extract was filtered and concentrated in vacuo using rotary evaporation, and the concentrated crude extract was applied to a C18 column (Polygoprep C18, 25–40 μm, 100 Å (Macherey-Nagel), 40 i.d ...
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bioRxiv - Cell Biology 2022Quote: ... MuSCs from the gaskin muscles were collected by FACS and then subjected to extract RNA with NucleoSpin® RNA XS kit from Macherey-Nagel. 1 ng high-quality RNA per sample were reverse transcribed and amplified into cDNA following Smart-seq2 library preparation protocol (Picelli et al. ...
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bioRxiv - Plant Biology 2021Quote: ... 5 µL of extracts were injected into a Nucleoshell RP 18 column (2.7 µm x 150 mm x 2 mm, Macherey-Nagel GmbH, Düren, Germany). A solvent system composed of A ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid preparation and DNA gel extraction were performed using the Nucleospin Plasmid kit and the Nucleospin Extract II kit (Macherey-Nagel, Lab Supplies Scientific SA, Hellas). DNA sequences were determined by Eurofins-Genomics (Vienna ...