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Citations for Takara Bio :
2101 - 2150 of 6767 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Human c-Jun sequence was amplificated using the cDNA of MCF7-BM02-1 and then transferred to pMXd3-PEF1-IRES-Puro vector using an In-Fusion Advantage PCR Cloning Kit (Clontech Laboratories Inc., CA, USA). The sequence encoding the transactivation domain of c-Jun in pMXs-Jun-IH was kindly provided by Dr ...
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bioRxiv - Neuroscience 2022Quote: ... qPCR expression analysis was conducted on cDNA derived from immunoprecipitated RNA of OT:RiboTag mice following reverse (SMARTer® PCR cDNA synthesis kit; Takara Bio Inc., Shiga, Japan).
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bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was then cloned into pVV16-hsp60 between NdeI and HinDIII using In-Fusion® HD cloning kit (Takara Bio USA, Inc) and Stellar™ competent cells ...
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bioRxiv - Immunology 2024Quote: ... reverse transcription and PCR steps were directly performed after sorting according to the SMART-Seq® v4 Ultra® Low Input RNA Kit protocol (Takara Bio, USA). Amplified complementary DNA were transferred to the Benaroya Research Institute (Seattle ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reactions at every examined depth were performed in triplicate (n=3) using Takara SpeedSTAR HS DNA polymerase kit (Takara Bio USA, Madison, WI) with the following modifications ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR fragments of spike protein DNA were cloned into linearized pMRNAXP vector using In-Fusion® HD Cloning Kit (Clontech® Laboratories, Inc.). The cloning mixtures were transformed to One Shot™ TOP10 Chemically Competent E ...
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bioRxiv - Cancer Biology 2022Quote: ... the viral supernatant was concentrated 10 times with Lenti-X concentrator (Takara). Media were changed at 24 hours after infection and antibiotics (2 μg/ml puromycin ...
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bioRxiv - Cell Biology 2022Quote: ... concentrated down 10 times using the Lenti-X Concentrator (631232; Takara Bio). Supernatants were kept at −80°C prior to being directly applied to target cells which were subsequently spun at 700 xg for 1 h at 37°C ...
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bioRxiv - Genetics 2021Quote: ... RT-qPCR reactions were performed in triplicate with TaqMan Master Mix: Premix Ex Taq (TaKaRa, Otsu, Japan) in a QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cancer Biology 2019Quote: ... then 500 ng of total RNA was reversely transcribed into cDNA using PrimeScriptTM RT Master Mix (TaKaRa) according to the manufacturer’s instructions in a total volume of 10 μL ...
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bioRxiv - Neuroscience 2020Quote: ... We then added 0.6 μL RT-mix [16.7 U μL−1 SMARTScribe Reverse Transcriptase (Takara Bio, 639538), 1.67 U μl−1 Recombinant RNase Inhibitor (Takara Bio ...
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bioRxiv - Microbiology 2020Quote: ... cDNAs from 1 μg DNase-treated purified RNA were obtained using PrimeScriptTM RT Master Mix (#RR036A, TAKARA) following the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2021Quote: ... synthesized cDNA with the ReverTra Ace qPCR RT Master Mix (TOYOBO) and used TaKaRa Ex Taq (TaKaRa) for amplification of NSP and MA genes ...
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bioRxiv - Microbiology 2020Quote: ... The synthesized cDNA was subjected to RT-qPCR using TB Green Premix Ex Taq II (Takara Bio). The PCR conditions were set as follows ...
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bioRxiv - Developmental Biology 2022Quote: ... Complementary DNA (cDNA) was prepared using the 5X Prime Script RT Master Mix (Takara RR-036A-1) with 1 μg of total RNA ...
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bioRxiv - Neuroscience 2022Quote: ... These RNA samples were then reverse transcribed into cDNA by PrimeScript™ RT Reagent (TaKaRa, RR036A, Japan). Real-time polymerase chain reaction (RT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... 2 μL of the RT reaction was combined with 2.5 μL of 10X Advantage 2 buffer (Takara), 2.5 μL of 2.5 mM dNTPs (Takara) ...
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bioRxiv - Plant Biology 2021Quote: ... then RNA was reverse-transcribed into cDNA using PrimeScript RT reagent with gDNA Eraser (Takara, Kusatsu, Japan). TB Green (Takara ...
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bioRxiv - Cell Biology 2021Quote: ... One-step qPCR was performed using One Step PrimeScript™ III RT-qPCR Mix (Takara, Kusatsu, Japan) with primers as follows ...
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bioRxiv - Microbiology 2021Quote: ... Samples were incubated 30 min at RT and loaded onto a 10 μL aliquot of Talon (Takara) resin ...
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bioRxiv - Neuroscience 2022Quote: ... Reverse transcription with 1 μg of total RNA was conducted using PrimeScript™ RT Master Mix (Takara) following the manual ...
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bioRxiv - Plant Biology 2022Quote: ... then RNA was reverse-transcribed into cDNA using PrimeScript RT reagent with gDNA Eraser (Takara, Kusatsu, Japan). TB Green (Takara ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was synthesized from 200 ng of total RNA by Prime script RT master Mix (Takara, RR036A). RT-qPCR was performed using TB Green Premix Ex TaqII (Tli RnaseH Plus ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA (400 ng) was then reverse-transcribed using PrimeScript RT Master Mix (#RR036A; TaKaRa, Kusatsu, Japan). The cDNA was then subjected to the subsequent PCR using TB Green Premix Ex™ Taq II (Tli RNaseH Plus ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was performed using a TB Green®Premix Ex Taq™II (TaKaRa, Lot: AL51019A), and β-actin gene was used as a reference gene ...
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bioRxiv - Microbiology 2023Quote: ... 500 ng of total RNA was then reverse transcribed in 10 µL reactions using PrimeScript RT (TAKARA) and random hexamer primers ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR quantitation of mRNAs was performed using TB Green™ Premix Ex Taq™ II (TaKaRa) with Applied Biosystems StepOnePlus Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The isolated RNA was reverse transcribed into cDNA using the PrimeScript™ RT Master Mix (Takara: RR036B) according to the the protocol provided by the manufacturer ...
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bioRxiv - Molecular Biology 2019Quote: ... and both long (NM_001300829) and short (NM_001280) isoforms of CIRBP were cloned by PCR (HiFi PCR premix, Clontech) from cDNA from Huh7 cells prepared with the Superscript III RT kit (Thermo Fisher ...
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bioRxiv - Plant Biology 2021Quote: ... The recombinant yeast strain was confirmed by PCR conducted with Matchmaker™ Insert Check PCR Mix (TAKARA, Japan). The full length of SlBES1.8 coding sequence was cloned into pGADT7 plasmid and subsequently transformed into the recombinant yeast strain ...
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bioRxiv - Biochemistry 2020Quote: ... The quantification of gene transcripts was performed by quantitative PCR (Q-PCR) using SYBR Premix Ex Taq (TAKARA). All values were normalized to the level of β-actin mRNA ...
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bioRxiv - Neuroscience 2020Quote: ... each cDNA was subjected to PCR genotyping using Emerald AMP HS PCR Master Mix (Takara Bio USA #RR330B) and primers shown in Supplementary Table S5 ...
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bioRxiv - Microbiology 2021Quote: ... and target genes were amplified by conventional PCR (50 µL reactions) with EmeraldAmp GT PCR Master Mix (Takara Bio ...
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bioRxiv - Plant Biology 2020Quote: ... Partial genomic sequences of CpPT1 were PCR amplified in these preparations using SapphireAmp Fast PCR Master Mix (Takara) and the primer pair citron_Fw and citron_Rv (Supplementary Table 8) ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was performed with the PrimeSTAR Max DNA polymerase 2x hot-start PCR master mix (TaKaRa Bio, R045A). PCR products were purified using the AMPure XP PCR purification system (Beckman Coulter ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products covering the whole sesRNA region was generated with CloneAmp HiFi PCR Premix (Takara, Cat. No. 639298), and purified with NucleoSpin Gel and PCR Clean-up kit (Takara ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.6 μl PCR mix was dispensed to each well containing the following: 1× SeqAmp PCR buffer (Takara Bio), 0.025 U μl−1 of SeqAmp polymerase (Takara Bio ...
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bioRxiv - Genomics 2020Quote: ... and 10 μL PCR Mixture (TaKaRa, Japan). PCR reaction was conducted on a T100 thermocycler (Bio-Rad ...
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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsbioRxiv - Molecular Biology 2020Quote: ... using PCR and In-Fusion cloning (Clontech). Human codon optimized Cas9 nuclease (hCas9 ...
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bioRxiv - Immunology 2021Quote: PCR and In-Fusion cloning (Takara Clontech) were used to generate ABCC1 constructs ...
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bioRxiv - Immunology 2021Quote: PCR and In-Fusion cloning (Takara Clontech) were used to generate ABCC1 constructs ...
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bioRxiv - Microbiology 2019Quote: ... PCR analyses were performed using ExTaq (Takara) according the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Using a PCR method and Pyrobest (Takara), the serine residues encoding S816 ...
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bioRxiv - Biochemistry 2020Quote: CloneAmp HiFi PCR Premix was from Takara. Restriction enzymes were from New England Biolabs ...
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bioRxiv - Cancer Biology 2022Quote: ... We then performed LaTaq PCR (Takara, RR002A) to amplify the β-catenin transcripts ...
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bioRxiv - Microbiology 2020Quote: ... with SYBR Green PCR Master Mix (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... with SYBR Green PCR Master Mix (Takara). The values were normalized to GAPDH expression.
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bioRxiv - Cancer Biology 2022Quote: ... using SYBR Green PCR Master Mix (Takara), and transcript levels were normalized to actin as an internal control ...
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bioRxiv - Genomics 2022Quote: ... PCR Master Mixkit was purchased from TaKaRa Company ...
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bioRxiv - Plant Biology 2019Quote: ... PCR was conducted using PrimeSTAR polymerase (Clontech) according to the manufacturer’s instructions ...