Labshake search
Citations for Takara Bio :
1951 - 2000 of 6767 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2020Quote: ... coli MG1655 genome by colony PCR and cloned into pR6K-PAmCherry-lox71-cm-lox66 using the In-Fusion® HD cloning kit (Clontech). Mutations were introduced using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) ...
-
bioRxiv - Genomics 2020Quote: ... PCRs were performed on board using 25 ng of DNA from each coral sample with the Advantage 2 kit (Takara Clontech) and a final primer concentration of 0.5 μM in a final reaction volume of 50 μl ...
-
bioRxiv - Developmental Biology 2021Quote: ... cDNA was amplified using 8 µl of the PCR MasterMix (SMART-Seq v5 Ultra Low Input RNA Kit for Sequencing, Takara Bio) with 25 cycles of amplification ...
-
bioRxiv - Biochemistry 2020Quote: ... The PfNEDD8 PCR product was cloned into the StuI-HindIII digested pSMO plasmid using in-Fusion® HD Cloning Plus kit (Takara) and transformed into DH5α E ...
-
bioRxiv - Neuroscience 2021Quote: ... The cloning reaction for pSPE3::H2B-GCaMp6s was performed using linearised PCR products and the In-Fusion HD Cloning Kit (Takara Bio). H2B-GCaMP6s mRNA was produced and precipitated using the mMESSAGE mMACHINE T3 kit (Life Technologies ...
-
bioRxiv - Neuroscience 2020Quote: ... single cells were sorted into individual wells of 8-well PCR strips containing lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara 634894) with RNase inhibitor (0.17 U/μl) ...
-
bioRxiv - Neuroscience 2020Quote: ... single cells were sorted into individual wells of 8-well PCR strips containing lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara 634894) with RNase inhibitor (0.17 U/μl) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Truncations of ENDOD1 and mutations of TP53 were obtained by fusing different PCR fragments using In-Fusion cloning kit (Clontech, 639650). Primers used in this study were listed in Supplementary Table S3.
-
bioRxiv - Molecular Biology 2020Quote: ... and hERG mutants were generated by PCR-mediated site-directed mutagenesis using an In-Fusion HD Cloning Kit (Takara, Otsu, Japan) as described previously (Kume et al. ...
-
bioRxiv - Molecular Biology 2020Quote: ... Amplicons were isolated from the agarose gel using the PCR cleanup and gel extraction kit (ClonTech, Takara USA, Mountain View, CA) and the nucleotide sequence of amplicons determined by Sanger direct sequencing (GENEWIZ ...
-
bioRxiv - Molecular Biology 2020Quote: ... Amplicons were isolated from the agarose gel using the PCR cleanup and gel extraction kit (ClonTech, Takara USA, Mountain View, CA) and the nucleotide sequence of amplicons determined by Sanger direct sequencing (GENEWIZ ...
-
bioRxiv - Microbiology 2020Quote: ... PCR amplification on the cleaned libraries was performed as follows: A reaction mix using LA Takara Hot Start kit (Takara Bio) was prepared according to the manufacturer’s instructions ...
-
Biosynthesis of Circular RNA ciRS-7/CDR1as Is Mediated by Mammalian-Wide Interspersed Repeats (MIRs)bioRxiv - Molecular Biology 2019Quote: ... The expression plasmids for circSPNS1 was constructed by subcloning the PCR-amplified fragments into FLAG-pcDNA3 plasmid using an In-Fusion HD cloning kit (Takara Bio) according to the manufacturer’s protocol ...
-
bioRxiv - Animal Behavior and Cognition 2019Quote: ... were designed to amplify the internal region of 11β-h and 11β-hsd2 gene respectively by using Takara PCR Amplification Kit (Takara, Japan). To obtain the full-length cDNA sequences ...
-
bioRxiv - Microbiology 2020Quote: ... The PCR products were purified and fused into a ClaI-digested plasmid with an In-Fusion HD cloning kit (TAKARA, Japan). Mutants for overexpressing HFB2 (TgOPB38530 ...
-
bioRxiv - Developmental Biology 2021Quote: ... 350 ng RNA samples in 6 µL nuclease-free water) were generated using the Clontech SMARTer smRNA-Seq kit using 8 PCR cycles (Takara Bio). 30 µL of the PCR reaction was purified with AMPure XP beads (Beckman Coulter A63880 ...
-
bioRxiv - Genetics 2020Quote: ... of which 16 reactions (200 ng in each) were amplified for 29 cycles and purified using Macherey Nagel Nucleospin Gel and PCR Purification kit (Takara Bio). Sequencing libraries were made of the 4C PCR products using Thruplex DNA-seq kit (Takara Bio) ...
-
bioRxiv - Genomics 2021Quote: ... reverse transcription and cDNA preamplification were performed in the C1 Fluidigm system using the SMARTer PCR cDNA Synthesis Kit (Clontech 634926) according to manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2022Quote: ... Donor vectors containing the GFP or mKate2 coding sequence flanked by 1kb homology arms adopted from both 3′ and 5′ sides of the insertion site were generated by cloning of PCR-amplified tags and arms into linearized pGEM-3z vector by In-Fusion HD Cloning Kit (Takara Bio). Obtained gRNA expression plasmids and donor plasmids were injected into the y w embryos with a final concentration of 120 ng/ul for each ...
-
bioRxiv - Biochemistry 2023Quote: ... Total RNA was further purified using mRNA Dynabeads and the purified mRNA was used as template for synthesis of cDNA using SMARTer PCR cDNA kit (Takara-Bio), with 18 cycles of amplification ...
-
bioRxiv - Cell Biology 2023Quote: RACE-ready (Rapid Amplification of cDNA 5’ Ends) cDNA was generated using SMARTer PCR cDNA Synthesis Kit (#634925, Clontech Laboratories, Inc.) according to the manufacturer’s protocol.
-
bioRxiv - Genomics 2023Quote: ... purified via gel extraction from a 1% agarose gel followed by cleanup using the NucleoSpin PCR clean up and gel extraction kit (Takara Bio). Linearized CROP-seq-optiI and amplified oligonucleotides were assembled using the NEBuilder HiFi DNA assembly cloning kit (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... linearized chimeric H3 HA sequence was gel purified using the NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
-
bioRxiv - Cell Biology 2023Quote: ... templates were constructed by cloning the 1.5 kb upstream and downstream homology arms into the pPD95.77 plasmid using the In-Fusion Advantage PCR cloning kit (Clontech, Cat. #639621). Fluorescence tags were inserted into the constructs with a flexible linker before the stop codons ...
-
bioRxiv - Evolutionary Biology 2023Quote: Complete cDNA sequences of taste receptors were obtained using a SMARTer 5’/3’ kit for RACE PCR to obtain 5’ sequence information missing from reference transcriptome (Takara, #634858). Briefly ...
-
bioRxiv - Biochemistry 2023Quote: ... coli BW25113 WT strain by PCR and inserted in pZA23 plasmid using the In-Fusion® HD Cloning Plus CE Kit (Takara).
-
bioRxiv - Molecular Biology 2024Quote: ... PCR products were analysed by gel electrophoresis and positive products were purified using the NucleoSpin Gel and PCR clean up kit (Clontech, Takara Bio) before being sent for sequencing by Eurofins Genomics (Germany) ...
-
bioRxiv - Biophysics 2024Quote: ... PCR products were overlapped and assembled to create Vpr-StayGold with In-Fusion Snap Assembly cloning kits (Takara Bio, no. 638947); successful insertion was verified by sequencing.
-
bioRxiv - Neuroscience 2023Quote: ... expressing UAS-eGFP was subjected to 14 cycles of PCR amplification (SMARTer Seq V4 Ultra Low Input RNA Kit; Takara Bio). 1 ng of amplified RNA was used to prepare cDNA libraries (Nextera XT DNA library preparation kit ...
-
bioRxiv - Plant Biology 2020Quote: CloneAmp HiFi PCR Premix (Takara) was used for all PCR amplifications ...
-
bioRxiv - Microbiology 2021Quote: ... 1× ExTaq PCR buffer (Takara, Clontech Laboratories ...
-
bioRxiv - Microbiology 2021Quote: ... using CloneAmp HiFi PCR (Takara) and was subcloned in the Thymidine kinase Cypridina plasmid (Thermofisher ...
-
bioRxiv - Molecular Biology 2019Quote: ... 10 μL PCR mixture (TaKaRa), 1 μL upstream primer (10 μmol/L) ...
-
bioRxiv - Biophysics 2023Quote: ... CloneAmp HiFi PCR Premix (Clontech) was used for all the PCR amplifications ...
-
bioRxiv - Plant Biology 2023Quote: ... PCR was performed by TaKaRa EX Taq (Takara ...
-
bioRxiv - Molecular Biology 2023Quote: ... Virus was concentrated 100 times by Lenti-X Concentrator (Takara) after filtering through a 0.45 syringe filter ...
-
bioRxiv - Microbiology 2020Quote: ... All PCR reactions were performed with CloneAmp HiFi PCR premix (Takara Bio, Mountain View, California) using 25 ng of DNA ...
-
bioRxiv - Cell Biology 2019Quote: ... The final PCR product was subcloned into pCS2+ by Infusion PCR (Takara Bio, Kusatsu, Japan). For subcloning into N’3xGFP-pCS2+ ...
-
bioRxiv - Developmental Biology 2023Quote: Linear DNA fragments were amplified via PCR using CloneAmp HiFi PCR premix (Takara Bio, 639298). PCR products were cut from agarose gels and purified using the Nucleospin gel purification kit (Takara Bio ...
-
bioRxiv - Developmental Biology 2023Quote: ... Linear DNA fragments were amplified via PCR using CloneAmp HiFi PCR premix (Takara Bio, 639298). PCR products were cut from agarose gels and purified using the Nucleospin gel purification kit (Takara Bio ...
-
bioRxiv - Molecular Biology 2020Quote: ... and cDNA was synthesized using PrimeScript™ RT Master Mix (Takara Bio Inc., Tokyo, Japan) as suggested by the manufacturers ...
-
bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized from 500 ng of total RNA using PrimeScript RT Master Mix (Takara). Quantitative real-time PCR (qPCR ...
-
bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed with TB Green Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa) on the StepOnePlus Real-time PCR System (Applied Biosystems ...
-
bioRxiv - Cell Biology 2019Quote: ... Total RNA or purified mRNA was reverse transcribed with PrimeScript™ RT Master Mix (Takara) using Oligo dT primers to obtain complementary DNA ...
-
bioRxiv - Molecular Biology 2019Quote: The isolated RNA was reverse transcribed into cDNA using the PrimeScript RT Master Mix (Takara). cDNA was amplified using the primer sets listed in Table S5 with SYBR Premix Ex Taq II (Takara ...
-
bioRxiv - Neuroscience 2021Quote: ... Total RNA (1 µg) was reverse-transcribed with PrimerScript™ RT Master Mix (Takara, #RR047A) after DNase I treatment (Takara ...
-
bioRxiv - Microbiology 2021Quote: ... RNA was reverse transcribed into cDNA with PrimeScriptTM RT Master Mix (Takara Bio, Shiga, Japan). Quantitative RT-PCR was performed with SYBR® Premix Ex Taq™ (Takara Bio ...
-
bioRxiv - Genetics 2021Quote: ... The purified RNA (total 30 μg) was reverse transcribed using PrimeScript RT Master Mix (Takara). On-target or off-target sites of genomic DNA or cDNA were amplified with KOD Multi & Epi PCR kits (Toyobo ...
-
bioRxiv - Microbiology 2022Quote: ... was performed using one-step Prime script III RT-qPCR mix (RR600A, Takara, Kyoto, Japan). The viral RNA of nucleocapsid protein was detected by a 2019-nCoV-N1 probe (10006770 ...
-
bioRxiv - Immunology 2022Quote: ... Reverse transcription to cDNA was performed with PrimeScrip RT Master Mix (RR036A, Takara Bio, Japan). The cDNA was used as a template for PCR amplification of the following gene coding sequences (primers shown in Supp ...