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Citations for Takara Bio :
2301 - 2350 of 7163 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... tricornutum were amplified by PCR was amplified by PCR using PrimeSTAR Max DNA polymerase (TaKaRa Bio Inc., Shiga, Japan) and the primer (pFs_nptII_1_F ...
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bioRxiv - Systems Biology 2022Quote: ... The DNA regions of interest were amplified from host plasmids by PCR using CloneAmp HiFi PCR Premix (Takara, 639298) with custom primers for Gibson assembly (with overhangs) ...
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bioRxiv - Biochemistry 2022Quote: ... N-terminal Flag-tagged P38α containing 3C protease site was amplified by PCR using CloneAmp HiFi PCR Premix (Takara) and ligated in to pcDNA3 using the aforementioned restrictions sites ...
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bioRxiv - Cell Biology 2020Quote: ... was used to PCR amplify the PLIN2 cDNA and gel purified via NucleoSpin Gel and PCR Clean Up (Takara, Mountain View ...
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bioRxiv - Microbiology 2024Quote: ... The guides were introduced into the pEcgRNA vector through reverse PCR using the CloneAmp HiFi PCR Premix (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of total RNA was reverse transcribed using Takara PrimeScript™ RT Master Mix (Clontech Laboratories, USA). qRT-PCR was performed on Step One Plus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was then performed using gene-specific primers (Table S1) and TB Green Premix Ex Taq (Takara). Levels of gene expression were normalized to that of 16S ribosomal RNA gene (OL4498 and OL4449 ...
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bioRxiv - Immunology 2021Quote: ... and quantitative reverse transcription-polymerase china reaction (RT-qPCR) was performed using SYBR Premix Ex Taq (TaKaRa, China). All procedures were performed following the manufacturer’s protocols.
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bioRxiv - Plant Biology 2019Quote: ... 500 ng RNA was DNAse I-treated (ThermoFischer Scientific) and submitted to reverse transcription (Takara PrimeScript™ RT). Before qPCR ...
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bioRxiv - Cell Biology 2021Quote: The mRNA and miRNA cDNA libraries are reverse-transcribed by PrimeScript™ RT Master Mix (TaKaRa, Dalian, China) and miRNA 1st Strand cDNA Synthesis Kit (by stem-loop ...
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bioRxiv - Cell Biology 2021Quote: ... First-strand cDNA was synthesized by reverse transcription of 1 μg RNA using PrimeScrip RT Master Mix(Takara). Quantitative real time-PCR was performed using TB Green Premix Ex Taq (Takara ...
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bioRxiv - Cell Biology 2021Quote: ... 2 µg of total RNA was subsequently reverse transcribed to cDNA using PrimeScript RT Master Mix (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... and reverse transcription was performed to generate cDNA using the PrimeScript RT Master Mix (RR036; Takara, Kusatsu, Japan). The RNase-Free DNase Set (79254 ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was then performed using gene-specific primers (Table 2) and TB Green Premix Ex Taq (Takara). Levels of gene expression were normalized to that of 16S rRNA and expression was assessed for each biofilm and each timepoint relative to its planktonic counterpart using the 2−ΔΔCt method (43).
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bioRxiv - Immunology 2020Quote: ... The reverse transcription of RNA into cDNA was performed using a Prime Script TM RT Master Mix (Takara). The following was the reaction system ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: cDNA was reverse-transcribed from 1 μg of total RNA using PrimeScript RT reagent (TaKaRa Biotechnology, Shiga, Japan), and 10 ng of cDNA was analyzed using SYBR Premix Ex Taq II (TaKaRa Biotechnology ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... The 2-μg RNA sample was reverse transcribed using the PrimeScript™ RT Master Mix (Takara, Shiga, Japan). Quantitative real-time PCR (qPCR ...
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bioRxiv - Microbiology 2021Quote: ... 500 ng of total RNA was used in 10 μL PrimeScript™ RT Master Mix (TaKaRa, Tokyo, Japan) as described in the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 500 ng of total RNA was reverse transcribed into cDNA using PrimeScript™ RT Master Mix (Takara, RR036A). qPCR analyses were performed using SYBR Premix Ex Taq™ II (Takara ...
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bioRxiv - Neuroscience 2022Quote: ... 4 uL Maxima 5x RT buffer, 0.124 uL NxGen RNAse inhibitor, 0.25 uL SUPERase In, 1 uL 10 mM Takara dNTPs ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative assessment of relative RNA expression was performed via RT-qPCR using SYBR Premix Ex TaqTM (TaKaRa, Japan). The reference gene ...
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bioRxiv - Microbiology 2023Quote: ... each RT reaction solution was amplified in 25 µl of SYBR Premix Ex Taq II (Takara Bio, Inc.) containing 0.2 µM of each primer ...
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bioRxiv - Immunology 2023Quote: ... RT-qPCR was performed using TB Green® Premix Ex Taq™ (Tli RNase H Plus) (Takara, RR420B) according to manufacturer’s instructions on an Applied biosystems StepOnePlus machine ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 400 ng of RNA was used to prepare cDNA using Primescript RT Master Mix (Clontech) at 42 °C following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... put on ice to form RNA/primer complex and mixed with 4.5 µL of RT solution {0.5 µL of SMARTScribe RTase (cat. #639536, Takara), 2 µL of 5x first strand buffer (supplied with RTase) ...
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bioRxiv - Bioengineering 2024Quote: ... The isolated RNA was reverse transcribed to cDNA following the manufacturer’s protocol (PrimeScript™ RT Master Mix, TaKaRa). The mRNA levels of RUNX2 (Hs00231692_m1) ...
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bioRxiv - Immunology 2020Quote: ... Filtered viral supernatants were concentrated 100 times using Lenti-X™ Concentrator (Takara Bio ...
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bioRxiv - Immunology 2022Quote: ... coated with 30 μg/mL Retronectin reused up to three times (T100B, Takara), at 2000 x g for 2 hours at 32°C and replaced with activated human CD8+ T cells in fresh RPMI supplemented with 30 U/mL IL-2 ...
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bioRxiv - Cell Biology 2019Quote: ... DNA was isolated from the expanded single colonies when confluent enough and used in PCRs using oligos to amplify exon 2 of CLN6 with CloneAmp HiFi PCR Premix (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The coding region of ADAR1 p150 was amplified by PCR and the resultant PCR product was inserted into a pEGFP-C1 vector (Clontech) using XhoI/BamHI restriction enzyme–recognition sites to generate the expression construct ...
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bioRxiv - Cell Biology 2021Quote: ... Semi-quantitative PCRs (sqPCRs) were performed on 3 μl of cDNA template using Emerald Amp Max PCR Master Mix (Takara). The sequences of the primers used for PCR amplifications are included in Supplemental Table 4.
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bioRxiv - Cell Biology 2021Quote: ... cDNA was further amplified by adding 40 μl of a PCR mix containing 0.03 U/μl of Terra PCR direct polymerase (Takara Bio), 1.25x Terra PCR Direct Buffer ...
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bioRxiv - Cell Biology 2021Quote: Site-directed mutagenesis of pLenti-CMV-Neo-PINK1 (C125G)-EYFP or pLVX-puro-OMA1 (E328Q)-EYFP was performed by PCR amplification (CloneAmp HiFi PCR Premix, Takara or Q5 High-Fidelity DNA Polymerase system ...
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bioRxiv - Biochemistry 2022Quote: ... The elute was diluted such that no more than 1.5ug of DNA was used per PCR reaction and amplified for 20 cycles of PCR using Primestar GXL (Takara Bio), run on a 1% agarose gel ...
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bioRxiv - Biophysics 2022Quote: ... eGFP-PolyQ31 was adapted from eGFP-PolyQ74 through the variability of Q-length PCR products amplified using CloneAmp HiFi PCR Premix (Takara). FM5-NPM1-mCh was generated as described in 27.
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bioRxiv - Molecular Biology 2020Quote: ... The sgRNA target locus on exon 3 or 5 was PCR amplified with Terra™ PCR Direct Polymerase Mix (Takara) for 35 cycles using primer sets mEX3F and mEX3R or mEX5F and mEX5R (Table 1) ...
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bioRxiv - Neuroscience 2022Quote: ... washed with 200 µL water, and resuspended in PCR mix (22 µL water, 25 µL Terra PCR direct buffer, 1 µL Terra Polymerase (Takara), 1 µL 100 M Truseq PCR handle primer ...
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bioRxiv - Cell Biology 2022Quote: All plasmids construction was performed by PCR amplification (CloneAmp HiFi PCR Premix) using appropriate primers followed by Gibson assembly (In-Fusion HD Cloning system, Clontech) into the EcoRI site of pLVX-puro vector (Clontech ...
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bioRxiv - Molecular Biology 2021Quote: ... A 150-250 bp region encompassing each targeted locus was PCR amplified from ∼40 ng genomic DNA with ends containing partial Illumina adapter sequences using CloneAmp HiFi PCR (Takara). Reaction conditions were as follows ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was amplified by PCR by adding 1 µl of the Terra PCR Direct Polymerase (1.25 U/µl, Takara 639270), 25 µl of the Terra PCR Direct buffer and 1 µl of the ISPCR primer (10 µM stock concentration ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplification with Gateway cloning compatible primers was performed directly on Aag2 gDNA using CloneAmp Hifi PCR pre-mix (Takara), without prior amplification in a TOPO TA cloning vector ...
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bioRxiv - Plant Biology 2022Quote: ... were chemically synthesised and cloned by PCR into the pJET1.2 based gRNA vectors using Clontech CloneAmp HiFi PCR Premix (Takara, Japan). The transformation vector pICU2:Cas9-dsRED containing the Cas9 gene from Streptococcus pyogenes expressed under the promoter of the Arabidopsis Incurvata 2 gene (ICU2 ...
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bioRxiv - Molecular Biology 2022Quote: Two hundred nanogram of DNAs were used as a template for polymerase chain reaction (PCR) by using EmeraldAmp MAX PCR mater mix (Takara). First 30 cycles of PCR were performed using forward and reverse primers below ...
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bioRxiv - Developmental Biology 2024Quote: ... Equimolar amounts of PCR products were mixed and a PCR was made with a primeSTAR GXL DNA polymerase (Takara Bio) without primers for the first 10 cycles using the following thermocycler conditions (95°C 3min ...
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bioRxiv - Microbiology 2024Quote: ... The deletion of each Stx2a phage in the CmR transformants was confirmed by colony PCR using EmeraldAmp MAX PCR Master Mix (TaKaRa) and specific primers ...
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bioRxiv - Biophysics 2024Quote: ... Kir2.1 pathological mutants (C154Y, R312H) were generated by polymerase chain reaction (PCR) with synthetic primers using the CloneAmp HiFi PCR Premix (Takara). Primer sets used for site-directed mutagenesis were the forward sequence ‘5-GTGACTGATGAGTACCCAATTGCAGTGTTTATGGTG –3’ ...
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bioRxiv - Molecular Biology 2023Quote: ... linearized by PCR and using the divergent primers reported in Supplementary Table 3 and CloneAmp™ HiFi PCR Premix (Takara) polymerase ...
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bioRxiv - Cancer Biology 2023Quote: ... The construct containing Y181G-coding point mutation was generated by inverse PCR of Zdhhc20WT plasmid using CloneAmp HiFi PCR Premix (Takara) and joining the ends of the PCR product using InFusion cloning.
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bioRxiv - Evolutionary Biology 2023Quote: ... The PCR products of the exons were assembled into one full-length sequence using overlapping PCR (In-fusion cloning, Clontech) for each TAS1R and were then subcloned into the pEAK10 expression vector (Edge Biosystems ...
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bioRxiv - Developmental Biology 2024Quote: ... CRISPR-targeted regions in marcks.L/S and marcksl1.L/S were PCR-amplified using the EmeraldAmp GT PCR Master Mix (Takara Bio). For every gene ...