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Citations for Takara Bio :
2251 - 2300 of 7163 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... One-step qPCR was performed using One Step PrimeScript™ III RT-qPCR Mix (Takara, Kusatsu, Japan) with primers as follows ...
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bioRxiv - Microbiology 2021Quote: ... Samples were incubated 30 min at RT and loaded onto a 10 μL aliquot of Talon (Takara) resin ...
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bioRxiv - Neuroscience 2022Quote: ... Reverse transcription with 1 μg of total RNA was conducted using PrimeScript™ RT Master Mix (Takara) following the manual ...
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bioRxiv - Plant Biology 2022Quote: ... then RNA was reverse-transcribed into cDNA using PrimeScript RT reagent with gDNA Eraser (Takara, Kusatsu, Japan). TB Green (Takara ...
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bioRxiv - Cancer Biology 2024Quote: ... The isolated RNA was reverse transcribed into cDNA using the PrimeScript™ RT Master Mix (Takara: RR036B) according to the the protocol provided by the manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was synthesized from 200 ng of total RNA by Prime script RT master Mix (Takara, RR036A). RT-qPCR was performed using TB Green Premix Ex TaqII (Tli RnaseH Plus ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA (400 ng) was then reverse-transcribed using PrimeScript RT Master Mix (#RR036A; TaKaRa, Kusatsu, Japan). The cDNA was then subjected to the subsequent PCR using TB Green Premix Ex™ Taq II (Tli RNaseH Plus ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was performed using a TB Green®Premix Ex Taq™II (TaKaRa, Lot: AL51019A), and β-actin gene was used as a reference gene ...
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bioRxiv - Microbiology 2023Quote: ... 500 ng of total RNA was then reverse transcribed in 10 µL reactions using PrimeScript RT (TAKARA) and random hexamer primers ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR quantitation of mRNAs was performed using TB Green™ Premix Ex Taq™ II (TaKaRa) with Applied Biosystems StepOnePlus Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Complementary DNA (cDNA) was generated from total RNA using PrimeScript RT Master Mix (Takara Bio Inc., Japan). RT-qPCR was performed on a Stratagene Mx3005P qPCR machine using a Takyon™ No ROX SYBR 2X MasterMix Blue dTTP (Eurogentec ...
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bioRxiv - Neuroscience 2024Quote: ... and anti-RFP antibodies (1/1000, RT, overnight, rabbit anti-DsRed, 632496, Takara Bio USA, Madison, WI), followed by Alexa-488 (1/100 ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized from the isolated RNA using the PrimeScript RT reagent (Takara Bio Inc., Shiga, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... 0.5 µl of 40 U/ µl RNase Inhibitor and 0.5 µl of the SMARTScribe RT (Takara, 639537), added the mix to the RNA mix and incubated the reaction at 42 °C for 60 min ...
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Aromatase in adipose tissue exerts an osteoprotective function in male mice via phosphate regulationbioRxiv - Physiology 2024Quote: ... RT-qPCR was performed using cDNA to evaluate gene expression with Thermal Cycler Dice (Takara Bio, Inc.) and SYBR Premix Ex Taq II (RR820S ...
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bioRxiv - Genomics 2020Quote: ... and 10 μL PCR Mixture (TaKaRa, Japan). PCR reaction was conducted on a T100 thermocycler (Bio-Rad ...
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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsbioRxiv - Molecular Biology 2020Quote: ... using PCR and In-Fusion cloning (Clontech). Human codon optimized Cas9 nuclease (hCas9 ...
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bioRxiv - Immunology 2021Quote: PCR and In-Fusion cloning (Takara Clontech) were used to generate ABCC1 constructs ...
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bioRxiv - Immunology 2021Quote: PCR and In-Fusion cloning (Takara Clontech) were used to generate ABCC1 constructs ...
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bioRxiv - Microbiology 2019Quote: ... PCR analyses were performed using ExTaq (Takara) according the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Using a PCR method and Pyrobest (Takara), the serine residues encoding S816 ...
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bioRxiv - Biochemistry 2020Quote: CloneAmp HiFi PCR Premix was from Takara. Restriction enzymes were from New England Biolabs ...
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bioRxiv - Cancer Biology 2022Quote: ... We then performed LaTaq PCR (Takara, RR002A) to amplify the β-catenin transcripts ...
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bioRxiv - Microbiology 2020Quote: ... with SYBR Green PCR Master Mix (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... with SYBR Green PCR Master Mix (Takara). The values were normalized to GAPDH expression.
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bioRxiv - Cancer Biology 2022Quote: ... using SYBR Green PCR Master Mix (Takara), and transcript levels were normalized to actin as an internal control ...
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bioRxiv - Genomics 2022Quote: ... PCR Master Mixkit was purchased from TaKaRa Company ...
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bioRxiv - Plant Biology 2019Quote: ... PCR was conducted using PrimeSTAR polymerase (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... or CloneAmp HiFi PCR Premix (Clontech, USA). Amplicons were separated on a 1-2% agarose gel and appropriate bands were excised and isolated using a gel extraction kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... and CloneAmp HiFi PCR (Takara, Tokyo, Japan) according to manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2022Quote: ... on a PCR thermal cycler (TP350, TaKaRa)57,58 ...
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bioRxiv - Plant Biology 2023Quote: ... or CloneAmpTM HiFi PCR Premix (Takara Bio) using the corresponding primers listed in TableS1 ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR was performed using TaKaRa ExTaq (TaKaRa). PCR products were analyzed using a 1% agarose gel ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR was performed using PrimeSTAR MAX (TAKARA) with primers (Supplementary Table 1) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR fragments containing pLib vector backbone (Clontech) and EF1A promoter ...
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bioRxiv - Molecular Biology 2023Quote: ... SapphireAmp Fast PCR Master Mix (Takara Bio) was used for verifying E.coli and M ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR was performed with ExTaq (TaKaRa, Japan) on 2720 thermal cycler (Applied Biosystems) ...
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Docking Domain Engineering in a Modular Polyketide Synthase and its Impact on Structure and FunctionbioRxiv - Biochemistry 2023Quote: CloneAmp HiFi PCR Premix was from Takara. Primers were synthesized by Sigma Aldrich ...
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bioRxiv - Genetics 2023Quote: ... Bisulfite PCR was performed using EpiTaq (Takara) using custom made locus specific primers containing 4 bp unique molecular identifiers (UMI ...
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bioRxiv - Molecular Biology 2023Quote: ... and SapphireAmp Fast PCR Master Mix (Takara). The PCR conditions were as follows ...
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bioRxiv - Developmental Biology 2023Quote: ... Nested PCR was performed with rTaq (Takara), followed by the PCR product fragments sequencing with Sanger sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... Extracted gDNA was PCR amplified (Takara, RR001A) using a 30-cycle protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Extracted gDNA was PCR amplified (Takara, RR001A) using a 30-cycle protocol ...
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bioRxiv - Microbiology 2024Quote: ... PCR was performed with Ex Taq (TaKaRa) under the temperature profile of an initial denaturation at 95°C for 2 min followed by 35 cycles of 95°C for 30 s ...
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bioRxiv - Physiology 2019Quote: ... to perform 5′ and 3′ rapid amplification of cDNA ends (RACE)-PCR utilizing the Clontech SMARTer 5′/3′ RACE Kit (Takara BIO USA Inc, CA, USA) as recently described58 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... library was prepared according to the Isoform Sequencing protocol (Iso-Seq) using the Clontech SMARTer® PCR cDNA Synthesis Kit (Clontech Laboratories (now Takara Laboratories), 634926 ...
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bioRxiv - Genetics 2020Quote: ... nucleotides 610–792 of the map3 ORF were subjected to random mutagenesis by using the Diversify PCR Random Mutagenesis Kit (Clontech Laboratories, Mountain View, CA, USA) in accordance with the manufacturer’s instructions for error-prone PCR ...
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bioRxiv - Systems Biology 2022Quote: ... The PCR products were subsequently cloned into pGEMHE Xenopus laevis oocyte expression vector using In-Fusion® HD cloning kit (TaKaRa Bio Inc, Kusatsu, JP) and the final sequence validated (Eurofin ...
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bioRxiv - Immunology 2023Quote: ... the library was prepared by synthesizing cDNA via reverse transcription and amplifying cDNA by long-distance PCR using the Takara SMARTer v4 kit (Takara Bio USA, San Jose, CA). 2×150bp sequencing was performed on an Illumina NovaSeq6000 instrument ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplicons were sized in gels (as above) and bands of interest isolated using the NucleoSpin Gel and PCR Clean-up kit (Takara Bio Inc., San Jose, CA). Sanger Direct Sequencing was performed at Azenta Life Sciences (South Plainfield ...