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Citations for Takara Bio :
1401 - 1450 of 2435 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Human CXCR4 was cloned into the pAcGFPm-N1 plasmid (Clontech Laboratories, Palo Alto, CA), as described (20).
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bioRxiv - Molecular Biology 2024Quote: The human embryonic kidney (HEK) 293T cell line was purchased from Takara (Cat# 632180) and cultured in a humidified 95% air / 5% CO2 incubator at 37°C in a standard Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Microbiology 2020Quote: ... Viral titer was determined by the ELISA p24 antigen assay (Lenti-X p24 Rapid Titer Kit, TaKaRa). Monocytes were infected immediately after purification by adding HIV-1BaL virus to the culture at MOI between 3 and 5 based on the p24 titer ...
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bioRxiv - Cell Biology 2022Quote: ... and complementary DNA of miR was synthesized using the Mir-X miRNA First-Strand Synthesis Kit (TaKaRa). RT-qPCR was performed with TB Green Premix Ex Taq II (Tli RNaseH Plus ...
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bioRxiv - Genomics 2020Quote: ... The titer of each virus was determined using the Lenti-X Go-Stix Plus kit (Takara Bio), according to the manufacturer’s instruction.
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bioRxiv - Immunology 2019Quote: ... and the pool of viral particles was concentrated using Lenti-X concentrator (Clontech Laboratories; cat. no. 631231). The titer of the GXMR-CAR viral particles was determined using HEK-293FT cells ...
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bioRxiv - Immunology 2019Quote: ... Viral titers were measured by a kit (Lenti-X p24 Rapid titer kit, #632200. Takara, Kusatsu, Japan). To suppress Mul1 expression ...
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bioRxiv - Cancer Biology 2019Quote: ... at a ratio of 2.5:1.0:0.6:0.5 to Lenti-X 293T cells (Takara, 632180, Kusatsu, Japan). A transfection reagent ...
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bioRxiv - Neuroscience 2020Quote: ... The lentiviral genome copy number was calculated using the Clontech Lenti-X qRT-PCR Titration kit (Takara).
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bioRxiv - Neuroscience 2021Quote: ... filtered through Whatman 0.45μm CA filter (GE #10462100) and concentrated using Lenti-X™ Concentrator (Takara #631232) according to the producer instructions (overnight incubation) ...
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bioRxiv - Neuroscience 2020Quote: ... Physical viral titer was determined using either Lenti-X qRT-PCR Titration Kit (Takara, Mountain View, CA) or qPCR Lentivirus Titration Kit (Applied Biological Materials Inc. ...
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bioRxiv - Neuroscience 2020Quote: ... The genome copy number was calculated using the Clontech Lenti-X qRT-PCR Titration kit (Takara Bio).
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bioRxiv - Neuroscience 2020Quote: ... The approximate titer of lentivirus was evaluated using a lentiviral titration kit (Lenti-X GoStix Plus; ClonTech). The lentiviral medium was transferred to 50 mL centrifuge tubes ...
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bioRxiv - Cell Biology 2021Quote: ... and checked for the presence of infectious virons by applying a sample to Lenti-X GoStix (Clontech). When cells reached 50% confluence ...
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bioRxiv - Cell Biology 2021Quote: A549 cells (purchased from JCRB cell bank, Japan (JCRB0076)) and Lenti-X 293T cells (Takara Bio, Japan) were cultured in high-glucose Dulbecco’s modified Eagle’s media (DMEM ...
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bioRxiv - Microbiology 2022Quote: ... the supernatant was collected and concentrated by Lenti-X™ Concentrator following the manufactures protocol (Clontech, CA). The pellet was resuspended in PBS ...
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bioRxiv - Microbiology 2022Quote: ... LASVpp-BlaM virus was concentrated 10 times with Lenti-X concentrator (Clontech Laboratories, Mountain View, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Virus containing supernatant was collected and virus particles were concentrated by Lenti-X concentrator (Clontech Laboratories, CA) according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2019Quote: Virus titer was determined by quantitative Polymerase Chain Reaction (qPCR) using Adeno-X qPCR Titration Kit (Clontech) on an Applied Biosystems 7900HT.
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bioRxiv - Microbiology 2020Quote: ... The purified virus was titrated with Lenti-X p24 Rapid Titer Assay (Takara Bio, Cat. No. 632200). The virus was stored at -80 °C for future use.
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bioRxiv - Neuroscience 2020Quote: ... Viral titer was determined using a qPCR Lentivirus Titration Kit (Lenti-X, qRT-PCR Titration Kit, Takara). For smaller scale virus preparation ...
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bioRxiv - Cancer Biology 2019Quote: ... The retrovirus was collected at 48h and 72h post-transfection and concentrated using Retro-X concentrator (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: A portion of the viral supernatant collected for infectivity assays was concentrated using the Retro-X (Clontech) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... Adenovirus was generated in HEK293A cells and titered using the Adeno-X Rapid Titer Kit (Takara Bio). For differentiation ...
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bioRxiv - Cancer Biology 2021Quote: ... or the Retro-X™ Tet-One™ Inducible Expression System (Puro) (TakaRa Bio USA, Cat #634307). The CSF3R construct was cloned into a Gateway-converted pMSCV-IRES-GFP vector (a gift from Tannishtha Reya ...
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bioRxiv - Cell Biology 2020Quote: ... and virus was collected 72 and 96 hours after transfection and concentrated using Lenti-X concentrator (Clontech). Cells were then transduced with virus after growing to 75% confluency and supplemented with 10μg/mL polybrene ...
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bioRxiv - Microbiology 2022Quote: ... Viral titer was determined by the ELISA p24 antigen assay (Lenti-X p24 Rapid Titer Kit, TaKaRa). Infections of fully differentiated MDM were performed after adherent growth in the presence of M-CSF for seven days ...
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bioRxiv - Microbiology 2023Quote: ... LASVpp-BlaM viruses were concentrated ten times with Lenti- X concentrator (Clontech Laboratories, Mountain View, CA, USA), snap-frozen and stored at - 80 °C.
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bioRxiv - Genetics 2023Quote: ... Viral harvesting and concentration was performed 48 hours post transfection using the Lenti X Concentrator (Clontech, 631231), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Large-scale production of high-titer lentivirus was generated using Lenti-X™ 293T Cells (TaKaRa, #632180) according to an established ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative PCR was performed using the Mir-X miRNA qRT-PCR TB Green Kit (Takara Bio, USA) using the protocol provided by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus titer was determined using the Lenti-X™ p24 Rapid Titer Kit (Cat: 632200, Clontech).
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bioRxiv - Molecular Biology 2023Quote: ... total RNA was used for cDNA synthesis with the Mir-X miRNA First-Strand Synthesis Kit (Clontech) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... viral particles were concentrated 20-fold to 400Lμl using Lenti-X Concentrator (Clontech, #631231, Mountain View, CA), and 100Lμl of concentrated virus was used to infect cells ...
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bioRxiv - Genetics 2023Quote: ... Plasmids that contain each sgRNA were transfected to HEK293T cells using Lenti-X packaging single shots (Takara) for viral packaging ...
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bioRxiv - Neuroscience 2024Quote: ... The preserved HEK medium was then spun down with 12.5 ml of lenti-X concentrator (Takara Bio), in an effort to capture the two distinct lentiviruses ...
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bioRxiv - Cancer Biology 2023Quote: ... LentiCas9-Blast and the custom sgRNA lentiviral library were transfected into Lenti-X 293T cells (Takara, 632180) respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviral particles were collected 72 hours after transfection and concentrated by using Lenti-X Concentrator (631231, Takara). After transduction ...
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bioRxiv - Biochemistry 2023Quote: ... The virus particles were further concentrated at 10X in volume using Lenti-X-concentrator (Clontech, PT4421-2) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The lentiviral titer was then determined using the Lenti-X p24 rapid titer kit (Takara Cat #632200).
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bioRxiv - Cell Biology 2023Quote: The lenti-CRISPR v2 constructs containing specific sgRNAs were transfected into Lenti-X 293T cells (Takara; 632180) with psPAX2 and VSV-G plasmids using the FuGENE HD transfection reagent (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... The viral titer was measured using a Lenti-X qRT‒PCR Titration Kit (#631235, TaKaRa, Shiga, Japan).
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bioRxiv - Molecular Biology 2020Quote: ... was amplified using specific primers (forward: 5’-AAGGAGATATACATATGCTCTCCGAAATGGTGGAAGAAG-3’; reverse:5’-GTGCGGCCGCAAGCTTTTATTTCTTTTTGTTGGTGGTCTG-3’) and cloned into pET28a using an InFusion HD Cloning Kit (Takara Bio USA) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: The transcription initiation and termination sites of OGRU were detected by 5’ and 3’ RACE using a SMARTer® RACE 5’/3’ Kit (Clontech, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... To determine the 5’ and 3’ UTR of the sds3 gene 5’ and 3’ Rapid amplification of cDNA ends (RACE) was performed using the SMARTer® RACE 5’/3’ Kit (Takara Bio) on RNA isolated using Trizol (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Primers used for amplifying the insert (5’-TACTTCCAATCCAATGTAGATATAAACAACAATAAGATTAGC-3’ and 5’- TTATCCACTTCCAATGAGATAACCTTGTACATCATCTGTATGC-3’) contained 15-bp overhang with homology to the plasmid for InFusion cloning (Takara Bio, 638947). The resulting plasmid ...
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bioRxiv - Microbiology 2021Quote: ... Primers used to amplify this region (5’-ATTGCGACACGTACTCTGCAGATCTCATACCATCATAGTTATAATATTAGC-3’ and 5’-AGAGGATCCCCATGGCTGCAGACACAGGTGTCGTCATTGTGA-3’) contained 15-bp overhang with homology to the plasmid for InFusion (Takara Bio, 638947) cloning and retained the Pst1 site ...
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bioRxiv - Microbiology 2023Quote: The 5’ and 3’ termini of the sRNA OueS were determined by the RACE assay (SMARTer RACE 5’/3’ kit, Takara Bio USA), adapted for non-poly-A-tailed RNA ...
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bioRxiv - Plant Biology 2022Quote: ... The qRT-PCR was performed with the reversed cDNAs as substrates and the MeSWEET10a specific primers (F: 5’-TCCTCACCTTGACTGCGCTG-3’; R: 5’-AGCACCATCTGGACAATCCCA-3’) by using the SYBR Premix Taq Kit (TaKaRa, Dalian, China) in the ABI7500 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... The 5 mL HisTALON cartridge (Clontech Laboratories, Cat# 635683) column was equilibrated with ten column volumes of Equilibration Buffer (HisTALON Buffer Set ...