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Citations for Takara Bio :
1251 - 1300 of 2560 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... Wild-type sequences were generated by PCR using human genomic DNA (Clontech) as a template ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... pooled total RNA of human kidney and liver were purchased from Clontech. Total RNA (2 µg ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 10 ng cDNA of various tissues (Takara Human MTC panel I & II) were used for each reaction and amplified by KOD Xtreme Hot Start DNA polymerase kit (Takara) ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA from human tissues was purchased from Clontech (catalog number 636643). Most of these samples represent pooled RNA from multiple individuals (between 2 and 63 individuals) ...
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bioRxiv - Neuroscience 2022Quote: ... were purchased from Addgene and Genscript or amplified from human adult and fetal brain RNA (Takara) (see Table S10)(Alford et al. ...
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bioRxiv - Cell Biology 2022Quote: ... ERManI-YFP was constructed with human ERManI cloned into peYFP-N1 (Clontech) using Hind III and Xma I yielding ERManI fused on its C-terminus to YFP through a 6 amino acid flexible linker (SGGGGS) ...
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bioRxiv - Cell Biology 2023Quote: Human CD34+ HSPCs were cultured overnight on RetroNectin-coated (Takara Bio USA) plates in StemSpan SFEM II medium supplemented with SCF 100 ng/mL ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from human liver and fetal brain was purchased from Clontech Laboratories ...
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bioRxiv - Developmental Biology 2024Quote: Human naïve cells were cultured in PXGL medium30: N2B27 medium (Takara, Y40002), supplemented with 1uM PD0325901 (Cambridge Bioscience ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were determined via the Bradford method using the TaKaRa Bradford Protein Assay Kit (Takara Bio, T9310A).
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bioRxiv - Plant Biology 2021Quote: Protein-protein interactions via yeast-two-hybrid (Y2H) assays were performed as described in the Matchmaker protocol (Clontech). HvRACB (amino acids 1-193 ...
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bioRxiv - Biochemistry 2023Quote: ... Uncleaved protein and cleaved His6-Ztag domains were separated from cleaved protein by incubation with TALON resin (Clontech). The supernatant was buffer exchanged to MES A buffer (20 mM MES ...
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bioRxiv - Plant Biology 2024Quote: ... This suspension was used to determine total protein concentrations using Takara BCA Protein Assay Kits (TaKaRa, Kusatsu, Japan).
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bioRxiv - Genomics 2019Quote: ... 4μl 5× First-Strand buffer (Takara, #639538), and 1μl B-tag-sw oligo ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... 5’-ctcgagtgcggccgcaagcttgctcatgacgctgccacggtg-3’ using PrimeSTAR HS (Takara). The pET28a was digested at NdeI and HindIII sites ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... 5’-cagtggtggtggtggtggtgctcgagtcaacacctggcgttgaccg-3’ using PrimeSTAR HS (Takara). The pET28a-MBP was digested at BamH1 and XhoI sites ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 μL dNTPs (25 mM; Takara Bio), 1 μL primers (10 pmol/μL each primer) ...
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bioRxiv - Immunology 2022Quote: 5 × 106 GP2-293 packaging cells (Clontech) were plated in a 10 cm dish containing D10 medium (DMEM supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2020Quote: ... 4µl 5× First-Strand buffer (Takara, #639538), and 1µl B-tag-sw oligo ...
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bioRxiv - Immunology 2022Quote: ... the SMARTer RACE 5’/3’ Kit (Takara) was used following manufacturer’s protocol and using the following primer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the 5’-Full RACE Core Set (TaKaRa) was used to extend Rtl6 mRNA from the mouse brain at 8 weeks of age ...
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bioRxiv - Microbiology 2021Quote: ... a SMARTer RACE 5’/3’kit (Takara) was used ...
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bioRxiv - Plant Biology 2022Quote: ... 5 × PrimeScript™ RT Master Mix (TAKARA) was used to synthesize cDNA ...
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bioRxiv - Developmental Biology 2023Quote: SMARTer RACE 5’/3’ Kit from Takara Bio was used following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Tissue protein lysates were purchased from Takara. Details for each lysate are shown in Table S1 ...
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bioRxiv - Neuroscience 2020Quote: ... cell-free protein expression system from Takara Bio Inc ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified using Talon resin (Clontech), eluted in 200 mM imidazole ...
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bioRxiv - Zoology 2020Quote: ... The protein concentration was measured by TaKaRa BCA Protein Assay Kit (Takara Bio ...
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bioRxiv - Molecular Biology 2019Quote: ... and protein was incubated with Talon (Clontech), 0.5 ml resin per litre cell culture for 1 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... the Capturem Protein A technology from Takara was employed according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... subtilis Secretory Protein Expression System (TAKARA Bio). For signal peptide library transformation into B ...
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bioRxiv - Cancer Biology 2021Quote: ... and concentrated using a Lenti-X™ Concentrator (631231, TaKaRa Bio Inc., Kusatsu, Shiga, Japan). Mia PaCa-2 cells and PCAFs were then transduced with the respective viral suspension at different multiplicity of infection (MOI ...
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bioRxiv - Cancer Biology 2021Quote: ... which ultimately was produced with Takara’s Lenti-X™ 293T cells (Clontech, cat. no. 632180) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: The Lenti-X Tet-On Advanced lentiviral inducible expression system (Clontech Laboratories, Mountain View, CA) was used to generate a doxycycline-inducible protein expression system in our stable OP9 FABP4-KO cell line ...
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bioRxiv - Genomics 2020Quote: ... Viral particles were collected 48 hours post-transfection and concentrated using Lenti-X Concentrator (Takara). Lentivirus was titrated in primary keratinocytes to maximize viral transduction while minimizing lentiviral toxicity ...
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bioRxiv - Molecular Biology 2022Quote: ... The viral titer was determined using Lenti-X™GoStix™ (Takara Bio Cat. #: 631280). HEK293T cells were transduced with lentivirus encoding the ABE reporter at varying dilutions (1:10 ...
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bioRxiv - Immunology 2019Quote: ... The viral supernatant was harvested on day three and concentrated with Lenti-X concentrator (Clontech). HL-60 Cells were spin infected at 2000 g for two hours at 32 ºC and then selected and maintained in medium supplemented with 1 µg/ml puromycin ...
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bioRxiv - Genetics 2019Quote: ... The lentiviral titers were determined with a Lenti-X™ qRT-PCR Titration Kit (Clontech).
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bioRxiv - Developmental Biology 2021Quote: ... Seventy percent confluent Lenti-X™ 293T cells (Takara Bio USA, Inc., Mountain View, CA) were transfected with Hdac3 lentiviral plasmids (Hdac3 KO or empty vector control [EV]) ...
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bioRxiv - Bioengineering 2021Quote: ... We detected p24 on lentiviral particles using Lenti-X GoStix Plus Kits provided by Takara Inc ...
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bioRxiv - Bioengineering 2021Quote: ... VSV-G-pseudotyped lentivirus was obtained by co-transfection of Lenti-X 293T cells (Takara) with pRRL-based transfer plasmids as well as pMD2.G (Adgene # 12259 ...
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bioRxiv - Cell Biology 2021Quote: ... Lentiviral particles were collected 72 h after transfection and concentrated with Lenti-X Concentrator (Clontech) and stored at − 80°C ...
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bioRxiv - Biophysics 2020Quote: ... The viral particles were then purified using the Adeno-X Maxi Purification Kit (Takara Bio) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 2 μL of lysis buffer (0.2% Triton X-100, 4U of RNase inhibitor, Takara) per well ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 ml of virus containing media were concentrated with Lenti-X concentrator (Takara Bio, 631231) to a titer sufficient to decrease pStat1 levels by 25% (Mean pStat1+ CD11b+ cells ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviruses were generated in HEK293FT cells and concentrated using the Lenti-X-concentrator (Takara Bio).
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bioRxiv - Neuroscience 2022Quote: ... and VSV-G (viral envelope) (Stewart et al., 2003) into Lenti-X HEK293T cells (Clontech) at a mass ratio of 5:4:3 using polyethyleneimine (PEI) ...
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bioRxiv - Immunology 2022Quote: ... lentiviruses in the culture supernatants were concentrated by using lenti-X concentrator (Takara bio, 631231). iBMDMs were seeded into 6-well plates overnight at a density of 1×105 per well ...
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bioRxiv - Molecular Biology 2021Quote: Lentivirus-containing supernatants from 48h and 72h post-transfection were concentrated with Lenti-X (Clontech).
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bioRxiv - Cancer Biology 2019Quote: ... Virus was collected after 48 hours and concentrated with Lenti-X Concentrator (Clontech, Cat#631231) (16) ...