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Citations for Takara Bio :
1551 - 1600 of 2560 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: The cDNA prepared from 10-day-old OsbZIP48OE seedlings was used to perform 5’ and 3’ RACE using SMARTer® RACE 5’/3’ Kit (Clontech, TaKaRa Bio, USA) as per manufacturer’s instructions followed by Sanger based sequencing of the RACE products ...
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bioRxiv - Microbiology 2023Quote: ... which was amplified by PCR from human cDNA using primers forward (5’-CAGTGTGGTGGAATTCACCATGTCAAGCTCTTCCTGGCT-3’) and reverse (5’-CACAAGATTTGGGCTCGGAAACAGGGGGCTGGTTAG-3’) and cloned into plasmid pVAX-IGHG1ΔCH1 by In-Fusion cloning (Takara Bio, San Jose, CA). This plasmid contains an Fc domain comprising Kabat numbers 226-478 of human IgG1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Duke University Medical Center) and HeLa Tet-Off (HTO; human, female, cervix; Clontech, RRID: CVCL_V352) cells were cultured in high-glucose ...
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bioRxiv - Neuroscience 2021Quote: ... The WT hPNPO cDNA was amplified from the human brain cDNA library (TaKaRa, Cat #637242) [24] ...
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bioRxiv - Developmental Biology 2021Quote: ... cryosectioned brains were stained to detect expression of human cytoplasmic marker STEM121 (TaKaRa, cat. Y40410) and human-specific GFAP marker STEM123 (TaKaRa ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus transduction was performed using RetroNectin® Recombinant Human Fibronectin Fragment (Takara, cat.no. T100A/B) according to the manufacturer’s instructions (with centrifugation) ...
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bioRxiv - Cell Biology 2020Quote: ... CHO-K1 cells were transfected with ACP-human IR plasmid using Xfect transfection reagent (Takara). 1 day after transfection ...
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bioRxiv - Neuroscience 2022Quote: ... Shortly, hiPSC lines (C1, C2, C3) and the commercial human iPSC-line Chipsc4 (Takara, Sweden) were cultured and differentiated into cortical NSCs as described elsewhere (23) ...
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bioRxiv - Cell Biology 2022Quote: Full length wild type human RORβ (NM_006914.3) was subcloned into the pLPCX retroviral vector (Clontech) using XhoI and NotI restriction enzymes (NEB ...
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bioRxiv - Cell Biology 2019Quote: PtK2 GFP-α-tubulin cells (stable line expressing human α-tubulin in pEGFP-C1; Takara Bio Inc. ...
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bioRxiv - Immunology 2021Quote: ... and cDNA encoding human full-length MuSK was cloned into pIRES2-EGFP plasmid vector (Clontech). AChR and MuSK vectors were kindly provided by Drs ...
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bioRxiv - Molecular Biology 2022Quote: ... The bait stain was combined with a Mate & Plate™ Human Heart library (Takara Bio) for 24 hours after which cells were plated on selective synthetic defined (SD ...
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bioRxiv - Neuroscience 2022Quote: ... and either 4 wells of 10 pg of Human Universal Reference Total RNA (Takara 636538) or 2 wells of 10 pg of Human Universal Reference and 2 wells of 10 pg Control RNA provided in the Clontech kit ...
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bioRxiv - Cell Biology 2023Quote: ... Aurora A and TPX2 were amplified from human testis cDNA (Marathon cDNA, Takara Bio Inc.) using Pfu polymerase (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... and SOX4 were amplified from a reverse transcript of Human Fetal Brain Total RNA (Takara) by high-fidelity PCR using Phusion DNA Polymerases (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... The ORF of human KIF23 was cloned within pCAX vector using In-Fusion system (TaKaRa). The disease associated c.755T>A mutation was introduced to human KIF23 cDNA by the PCR-based mutagenesis as described (Xia et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... mRNA was utilized with the SMARTer Human TCR a/b Profiling Kit v2 (Takara, USA). The resulting libraries were sequenced for paired-end reads of 2×250 bp on an Illumina system at a sequencing depth of 7.5X.
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bioRxiv - Plant Biology 2023Quote: ... These plasmids were transformed into the yeast strain AH109 for testing protein-protein interaction using the Matchmaker Gold system (Takara Bio, Kusatsu, Japan). Yeast transformation and growth were performed as described in [Pecher et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... The collected media containing virus was filtered through 0.45μm pore filter and mixed with Lenti-X concentrator (Clontech) as indicated by manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2020Quote: ... we used jetPrime (Polyplus) according to the manufacturer’s instructions to transfect Lenti-X 293 T HEK cells (Takara) with lentiviral production plasmids pMD2.G and psPAX2 along with our individual transfer plasmids ...
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bioRxiv - Immunology 2021Quote: ... GRV particles were produced by transient transfection of Phoenix GP cells and concentrated using Retro-X concentrator (Takara). Sorted HSPC were pre-expanded for two days ...
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bioRxiv - Bioengineering 2021Quote: ... The virus titre was calculated using the ELISA-based Lenti-X™ p24 Rapid Titer Kit (Takara Bio) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... cell content was ejected onto 1.1 µl drop of lysis buffer (0.1% Triton X-100, 2 U/µl TaKaRa RNAse inhibitor ...
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bioRxiv - Cancer Biology 2020Quote: ... pooled and filtered through a 0.45μm syringe filter and then concentrated using the Lenti-X Concentrator solution (Clontech). Freshly concentrated supernatants were added directly to drained sub-confluent recipient cells and incubated overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Viral supernatant was collected at 48 and 72 hours post transfection and concentrated using Lenti-X Concentrator (Takara). Viral titer was established by serial dilution in relevant cell lines and readout of %BFP+ by flow cytometry ...
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bioRxiv - Microbiology 2020Quote: ... Pseudovirus-containing supernatants were collected 48 h later and concentrated using Lenti-X™ concentrator kit (Takara, CA). Pseudovirus neutralization assay was then performed by incubation of SARS-CoV-2 pseudovirus with 5 folds serially diluted antibody with starting concentration of 1 μg/ml for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... media was collected and filtered to remove cells and debris and then complexed with Lenti-X concentrator (Clontech) according to the manufacturer’s protocol to concentrate lentiviral particles ...
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bioRxiv - Cell Biology 2021Quote: ... cells were co-transfected with viral vector and Lenti-X HT packaging mix (Clontech, 15μl per 3μg vector) using a jetPEI system (Polyplus transfection ...
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bioRxiv - Biophysics 2020Quote: U2OS (a kind gift from Mark Groudine lab, Fred Hutchinson Cancer Research Center) and Lenti-X 293T (Takara) cells were cultured in growth medium consisting of Dulbecco’s modified Eagle’s medium (GIBCO) ...
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bioRxiv - Cancer Biology 2021Quote: ... Viral titer was assessed upon collection and concentration of lentiviral supernatant with Lenti-X Concentrator (Takara Biotech; #631231). Appropriate final concentrations were chosen to maintain MOI < 0.3 to reduce probability of coinfection with two or more sgRNA sequences ...
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bioRxiv - Systems Biology 2022Quote: ... and deposited in 10 uL ice-cold lysis buffer containing 0.1% Triton-X 100 and RNase inhibitor (Takara) and stored at −80°C until further processing ...
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bioRxiv - Cell Biology 2022Quote: ... inducible cell lines were generated using the Lenti-X Tet-On-Advanced Inducible Expression System (Clontech Laboratories, Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... filtered through Whatman 0.45 µm CA filter (GE #10462100) and concentrated using Lenti-X™ Concentrator (Takara #631232) according to producer datasheet (overnight incubation) ...
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X-ray mediated scintillation increases synaptic activity via Cerium-doped LSO and Channelrhodopsin-2bioRxiv - Neuroscience 2020Quote: ... showing a viral titer of >106 IFU/ml as measured by Lenti-X GoStix (Takara, Mountain View, CA). To develop stably expressing OptoXR-EYFP cells ...
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bioRxiv - Cell Biology 2022Quote: ... achieving 10x concentrated preparations of which the lentiviral titer was determined using Lenti-X GoStix Plus (Takara, 631280). Lentiviral preparations were aliquoted and stored at -80°C until use.
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... together with packaging vectors pMD2.G (4.5 μg) and psPAX2 (13.5 μg) were delivered into lenti-X 293T cells (Clontech) using 108-144 μg PEI MAX 40K (Polysciences ...
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bioRxiv - Cancer Biology 2021Quote: ... Lentivirus was collected in the supernatant and filtered prior to concentrating with Lenti-X™ Concentrator (Clontech 631231) manufacturer protocol.
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bioRxiv - Immunology 2021Quote: ... Viral supernatant was collected and concentrated using Lenti-X Concentrator 24- and 48-hours after the transfection (Clontech). Jurkat 76.7 cells (a gift from Wouter Scheper ...
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bioRxiv - Neuroscience 2022Quote: All viruses were made using the 2nd generation lentiviral packaging systems in Lenti-X HEK293 FTT cells (Takara). Lenti-X cells were passaged maximum 3 times before being used for virus production in HEK media (High glucose DMEM with 4 mM GlutaMAX ...
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bioRxiv - Cell Biology 2022Quote: ... Viral containing supernatant was collected at 96 hr post transfection and concentrated using Lenti-X concentrator (Takara; 631232). Collected lentivirus was tittered using Lenti-X™ GoStix™ Plus (Takara;631280) ...
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bioRxiv - Cancer Biology 2022Quote: ... with lentivirus for 8h at an optimized dilution determined with the Lenti-X GoStix Plus protocol (Takara, 631280). CHAC1 and SLC25A39 knockout cells were generated using the pLenti-CRISPR-V2 plasmid (Addgene ...
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bioRxiv - Cell Biology 2022Quote: ... and either used on the same day or concentrated using Lenti-X concentrator solution (Takara Bio, Cat #631231) and snap frozen at −80C.
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bioRxiv - Genetics 2022Quote: ... 0.1-0.2*106 mESCs were seeded per well in a 24-12-well plate in conventional ESC medium and transduced the next day with 0.25-0.5 ml of 10:1 concentrated (lenti-X, Clontech) supernatant with 8 ng/μl polybrene (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... the cell body was immediately sucked into the glass electrode with negative pressure and expelled onto a 1.1 µl drop of the ice-cold lysis buffer (0.1% Triton X-100, 2 U/µl TaKaRa RNAse inhibitor ...
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bioRxiv - Neuroscience 2022Quote: ... Media containing viral particles was sterile-filtered and concentrated using the Lenti-X™ Concentrator (Takara Bio, 631231), according to manufacturer instructions.
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bioRxiv - Physiology 2024Quote: ... Three volumes of concentrated virus medium were subsequently mixed with one volume of Lenti-X concentrator (Takara Bio) and rotated at 4°C overnight before centrifugation (1500 g for 45 min at 4°C) ...
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bioRxiv - Cell Biology 2024Quote: ... 7 μg of lentiviral vectors was mixed with a Lenti-X packaging (VSV-G) single shots tube (Clontech) in a final volume of 600 μl for 15 min before the transfection mix was added to subconfluent (80-90% ...
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bioRxiv - Cell Biology 2023Quote: ... Virus containing media was collected from the LentiX-293T cells and concentrated using Lenti-X Concentrator (Takara Bio). pLVX-Tet3G virus and polybrene was added to L6-myoblast cells and cells were positively selected using neomycin to create Tet3G expressing cells ...
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bioRxiv - Cell Biology 2023Quote: ... the media was collected and viral particles were concentrated using Lenti-X™ Concentrator (Takara Bio Inc., 631231). For transduction ...
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bioRxiv - Immunology 2024Quote: ... A stable IFN-λ-expressing cell line was obtained by transfection of one million Lenti-X (HEK293T, Takara) cells with 500 ng Super PiggyBac Transposase vector and 1250 ng of PiggyBac transposase mammalian expression vector using Lipofectamine 3000 following to the manufactureŕs instructions ...