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Citations for Takara Bio :
1651 - 1700 of 2435 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... spent medium was collected and filtered through a 0.45 μm cellulose acetate filter before concentrating the supernatant using Lenti-X™ concentrator (TaKaRa).
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bioRxiv - Microbiology 2022Quote: ... The plasmids were extracted from all the colonies which grew on QDO/X/A agar plate using Easy Yeast Plasmid Isolation Kit (TaKaRa). PCR was carried out using the extracted plasmids as template ...
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X-ray mediated scintillation increases synaptic activity via Cerium-doped LSO and Channelrhodopsin-2bioRxiv - Neuroscience 2020Quote: ... 36-48 h post transfection lentivirus containing media was harvested and concentrated using Lenti-X concentrator (Takara, Mountain View, CA) as per manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: ... The lentivirus-containing media was harvested 48 or 72 h after transfection and concentrated 40 – 50 times using Lenti-X concentrator (Takara Clontech). Sorted Treg cells were stimulated with plate-bound anti-CD3 (1 μg/ml ...
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bioRxiv - Genomics 2019Quote: ... The transfected cells were cultured for 2 days and lentivirus were harvested and concentrated using the Lenti-X concentrator (Takara) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated up to 48 hrs and then lentivirus-containing media was collected and concentrated with Lenti-X concentrator (Clontech). To transduce W4 cells in T25 flasks ...
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bioRxiv - Immunology 2019Quote: ... All cell lines were obtained directly from the ATCC (with exceptions of Lenti-X 293T cells, which were obtained from Clontech Laboratories ...
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bioRxiv - Cell Biology 2019Quote: ... and lentiviral Tre3G-Cas9 and Tet3G particles were produced in HEK293T cells using the Lenti-X HT packaging system (Clontech). Transduced cells were selected with 10 μg/mL puromycin and 400 μg/mL G418 ...
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bioRxiv - Immunology 2020Quote: ... The lentivirus or Vpx-VLPs containing media was harvested 72 h after transfection and concentrated 80 times using Lenti-X concentrator (Takara Clontech) or Lenti Concentrator (Origene) ...
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bioRxiv - Developmental Biology 2021Quote: ... Lentiviral production was performed as previously described (McCaffrey and Macara, 2009) and viruses were concentrated using Lenti-X Concentrators (Takara).
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bioRxiv - Microbiology 2021Quote: ... the pLVX-ORF3-E plasmid was transfected into HEK-293T cells using the Lenti-X Packaging Single Shots kit (Takara). Lentiviral supernatants were harvested at 72 h post-transfection and filtered through a 0.22 μM membrane (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... The infectious titer of lentivirus was determined by a Lenti-X™ qRT-PCR Titration Kit (Clontech, Mountain View, CA).
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Neutralizing activity of broadly neutralizing anti-HIV-1 antibodies against primary African isolatesbioRxiv - Immunology 2020Quote: ... and the presence of p24 in the culture supernatant was quantified by the Lenti-X p24 Rapid Titer kit (Clontech) after 7 ...
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bioRxiv - Immunology 2020Quote: ... Filtered viral supernatants were concentrated 100 times using Lenti-X™ Concentrator (Takara Bio, Clontech, Saint Germain en Laye, France) and resuspended in phenol red-free medium ...
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bioRxiv - Immunology 2021Quote: ... 293T Lenti-X cells were seeded onto 10-cm Petri dishes and transiently transfected (CalPhos™ Mammalian Transfection Kit, Clontech Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... The biosensor expression cassette was transduced into the stable tet-OFF MCF10A cell line under the regulation of a tet-inducible minimal CMV promoter (pRetro-X-Puro; Clontech) and selected using puromycin for a stably integrated population ...
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bioRxiv - Cancer Biology 2022Quote: Viral particles were generated by transfecting the lentiviral construct into HEK-293T cells using Lenti-X Packaging Single Shots (Clontech). 48 hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were clarified by centrifugation at 3,000 x g and incubated with Talon® immobilized metal affinity resin (Takara #635502) for 2 hours at 4ºC ...
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bioRxiv - Microbiology 2022Quote: ... The resultant lysate was cleared by centrifugation at 30 000 x g for 20 min and applied to Talon CellThru Co2+ chelating resin (TaKaRa-Clontech). The protein bound resin was washed with 3 column volumes (CV ...
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bioRxiv - Immunology 2022Quote: ... following manufacturer’s instructions with a 1.5:1.5:1 μg ratio of the SFG:Peq-Pam:RD114 DNA plasmids and concentrated using Retro-X™ Concentrator (Takara, Kusatsu, Japan) following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Culture supernatants containing lentivirus were collected 24 and 48 h after transfection and concentrated by a Lenti-X concentrator (Takara) followed by passing through a 0.45-μm PES filter ...
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bioRxiv - Molecular Biology 2022Quote: ... The titer of harvested lentivirus was established using the Lenti-X RT-qPCR Titration Kit (Takara Bio, Cat. no. 631235). A representative calibration plot and LV tittering of pAIO is added as Supplementary data S4.
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bioRxiv - Immunology 2022Quote: ... T cells were infected with either replication-competent HIV-1 (pAD8gNef [80] or pNLgNef [79] or single-round HIV-1 (pHR-CD8a-d2eGFP-IRES-Nef) using Lenti-X Accelerator (TaKaRa) following the manufacturer’s protocol.
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bioRxiv - Physiology 2023Quote: ... Lentivirus was collected beginning 48-hrs post-transfection and concentrated with Lenti-X Concentrator (Takara Bio USA, Mountain View, CA) for 3 consecutive days ...
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bioRxiv - Cancer Biology 2023Quote: ... Additional lentiviral vectors were packaged in HEK 293T cells using Lenti-X Packaging Single Shots (VSV-G) (Takara Bio USA) per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T packaging cells were transfected with the required lentiviral cDNA constructs using Lenti-X packaging system following the manufactures protocol (631276, Takara). SKM cells stably expressing HA-GLUT4-GFP (SKM-GLUT4 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... the supernatants of infected cells were collected and infectious virus particles were measured using the Adeno X Rapid Titration Kit (Takara Bio Europe ...
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bioRxiv - Biochemistry 2023Quote: ... the insoluble material was removed by 50k x g centrifugation for 30 min and the supernatant was added to Talon resin (Clontech) with 20 mM imidazole to bind overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Lenti-X HEK293T cells were transfected with either pLVX-NME6Myc-Flag or pLVX-NME6H137N Myc-Flag using Lenti-X Packaging Single Shots (Takara). The viral supernatant was collected after 48 h ...
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bioRxiv - Cell Biology 2022Quote: ... The viral supernatant was passed through a 0.45 um filter and concentrated to 1:66 with Lenti-X concentrator (631231, Takara Bio) in house or to 1:255 by ultracentrifugation in the core.
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bioRxiv - Genomics 2023Quote: ... The transfected cells were cultured for 2 days and lentivirus were harvested and concentrated using the Lenti-X concentrator (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1%Triton X) and then resuspended in lambda DNA solution (diluted to approximately 20 ng/µL in TE solution, 3010, Takara). For the Sera-Mag™ Select (29343052 ...
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bioRxiv - Immunology 2023Quote: ... Lentiviral particles were produced by co-transfecting the barcoding plasmid with the packaging and envelope plasmids pMD2G and pCMV-dR8.91 (provided by Andrew Ramos) into Lenti-X 293T cells (Takara, 632180) with Lipofectamine 2000 (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: Lentivirus was produced using a standard protocol64 from a 15 cm plate of 25M lenti-x 293T cells (Clontech #632180) via polyethylenimine (PEI ...
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bioRxiv - Cancer Biology 2023Quote: ... The generated lentiviral plasmids were cotransfected with viral packaging plasmids PAX2 and pMD2.G into HEK 293T Lenti-X cells (Clontech) using TransIT-LT1 (Mirus Bio LLC ...
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bioRxiv - Cancer Biology 2023Quote: ... The number of virus copies/ml was determined using the Lenti-X™ qRT-PCR Titration Kit (Takara Bioscience, 631235) according to the manual ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were grown for 72 hours post-transfection and the virus was concentrated ∼40-fold from the supernatant using the Lenti-X concentrator kit (Takara) per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Growth media was changed the following day and lentiviruses-containing supernatants were harvested at 72 hr after transfection and concentrated 100-fold using Lenti-X concentrator (Clontech). For infection ...
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bioRxiv - Cell Biology 2024Quote: ... in a final volume of 600 μl for 15 min before the transfection mix was added to subconfluent (80-90%) Lenti-X 293T cells (Clontech) grown on a 10-cm tissue culture dish ...
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bioRxiv - Immunology 2024Quote: An adenovirus plasmid encoding the first 30 amino acids of KRAS with the G12V mutation (KRAS G12V-30) was created following the manufacturer’s instructions (Adeno-X CMV, Takara Bio). Adenovirus particles were produced by transfecting the adenoviral plasmid into HEK293 cells ...
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bioRxiv - Genetics 2024Quote: ... 0.2*106 mESCs were seeded in a well of a 12-well plate in 2iL and transduced the next day with 1ml of 5x concentrated (lenti-X, Clontech) and filtered viral supernatant with 8 ng/μl polybrene (Sigma Aldrich) ...
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bioRxiv - Immunology 2023Quote: Lentivirus was generated by co-transfecting the barcoding plasmid with the packaging and envelope plasmids pMD2G and pCMV-dR8.91 (provided by Andrew Ramos) into Lenti-X 293T cells (Takara, 632180) using Lipofectamine 2000 (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: Media was collected 48 h after transfection and clarified by syringe filtration through a 0.45 µm polyvinylidene difluoride membrane (Millex-HV) before concentrating the virus with a Lenti-X concentrator (Clontech 631231). The concentrated virus was resuspended in PBS and aliquoted into single-use tubes stored at −80 °C ...
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bioRxiv - Microbiology 2023Quote: ... the pLVX-G9R+A1L-puromycin or pLVX-G9R+A1L-blasticidin plasmids were transfected into 293T cells using the Lenti-X Packaging Single Shots kit (631275, TaKaRa). Lentiviral supernatants were harvested 48 hours post-transfection and filtered through a 0.45 μm membrane (SLHV033RB ...
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bioRxiv - Cancer Biology 2024Quote: ... with psPAX2 and pMD2.G viral particles harvested during the 16-72 h window post-transfection were concentrated with Lenti-X (Clontech) and stored at -80°C ...
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bioRxiv - Bioengineering 2024Quote: ... filtered with a 0.45μm filter to remove cellular debris and concentrated (100x) using Lenti-X concentrator (Takara, San Jose, CA). The resulting particles were resuspended in DMEM with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... iSLK and iSLK-BAC16 cell line derivatives were generated using 2nd generation lentivirus as follows: 1×106 Lenti-X 293T cells (Clonetech/Takara via the UC Berkeley Tissue Culture Facility ...
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bioRxiv - Developmental Biology 2021Quote: ... the RNA-protein mix was catalyzed by proteinase K (Takara, 9034) at 55 °C for 1 h ...
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bioRxiv - Genomics 2019Quote: ... and proteins extracted with affinity purification using Cobalt-Talon beads (Clontech). Site-directed mutagenesis for Exd and Hth was performed via amplification of the original plasmid with primers harboring single amino acid replacements (arginine to alanine ...
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bioRxiv - Cell Biology 2022Quote: ... E.coli competent cells transformed with pTf16 chaperone protein tag (TaKaRa Bio) per the manufacturer’s protocol.