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Citations for Takara Bio :
1601 - 1650 of 2435 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... the solution was replaced with a 0.5% BSA and 0.2% Triton X/PBS solution (PBST) containing a 1:1,000 dilution of Rabbit anti-DsRed (Takara, #632496) or Chicken Polyclonal anti-GFP antibody (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... mouse MEFs (RIKEN BioResource Center, Tsukuba, Japan), mouse RAW264.7 macrophages (RIKEN BioResource Center, Tsukuba, Japan) and Lenti-X 293T (Clontech) were cultured at 37°C in 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Biophysics 2024Quote: Lentivirus titration was determined by p24 ELISA using Lenti-X™ p24 Rapid Titer Kit (Takara Bio, no. 632200) and a plate reader (PerkinElmer Victor3 V) ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse-transcription-quantitative PCR (RT-qPCR) was conducted using the Mir-X miRNA qRT-PCR TB Green Kit (TAKARA), the reverse-transcription samples ...
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bioRxiv - Genomics 2024Quote: ... Supernatant containing viral particles was harvested after 2 and 3 days and concentrated 100x using Lenti-X concentrator (Takara) following manufacturers instructions.
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bioRxiv - Microbiology 2024Quote: ... Lentiviral supernatant was harvested 48 h later and then filtered to be concentrated through a Lenti-X Concentrator (Takara) following manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... to perform 5′ and 3′ rapid amplification of cDNA ends (RACE)-PCR utilizing the Clontech SMARTer 5′/3′ RACE Kit (Takara BIO USA Inc, CA, USA) as recently described58 ...
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bioRxiv - Molecular Biology 2020Quote: RACE assay was performed with SMARTer RACE 5’/3’ kit (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: The assay was performed using the 5’-Full RACE kit (TaKaRa) according to the manufacturer’s instructions with modifications ...
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bioRxiv - Neuroscience 2021Quote: ... coli BL21 and purified on 5 mL Talon column (Clontech®) loaded with Cobalt ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysate was incubated with 5 mL TALON beads (Takara Bio), washed with 150 mL lysis buffer and eluted in 22 mL of elution buffer (25 mM Hepes pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... and deoxyribonuclease I (DNase I, 5 units/mL, Takara, Shiga, Japan) for 15 min ...
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bioRxiv - Cell Biology 2019Quote: ... containing 5 μl of 2× SYBR Premix Ex Taq II (TaKaRa), 1 μl of cDNA ...
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bioRxiv - Cell Biology 2019Quote: ... containing 5 μl of 2× SYBR Premix Ex Taq II (TaKaRa), 1 μl of cDNA ...
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bioRxiv - Immunology 2019Quote: ... The diafiltrated medium was loaded onto 5 ml Talon resin (Clontech), washed with 10 CV of 250 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... 5’ RACE was performed with SMARTer RACE cDNA Amplification Kit (Clontech). IgG /IgK/IgL NGS libraries were made by using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Neuroscience 2021Quote: ... The SMARTer® RACE 5’/3’ Kit was purchased from Clontech Laboratories ...
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bioRxiv - Microbiology 2020Quote: ... and 5 µL of the ligation mix (Takara Ligation Kit 6023), and then placing the tubes into a heat block at 90 °C for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl 2×SYBR®Premix Ex Taq™ II (TaKaRa), 0.75 μM primers and nuclease-free water to 20 μl ...
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bioRxiv - Plant Biology 2022Quote: ... 2 μL of 5× PrimeSTAR GXL Buffer (Takara Bio, Kusatsu, Japan), 1.0 μL of PrimeSTAR GXL DNA Polymerase (1.25 U/μL) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with D/D-Solubilizer (5 μM, Clonetech/Takara) and Cycloheximide (35.54 μM ...
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bioRxiv - Evolutionary Biology 2023Quote: ... SMARTer® RACE 5’/3’ Kit was used (Takara Bio, Japan). Prior to the reaction ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were loaded on 5 mL of TALON beads (Takara Bio) pre-equilibrated with the lysis buffer ...
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bioRxiv - Immunology 2020Quote: ... Activated NK cells were transduced with retroviral supernatants on day +4 in human fibronectin-coated plates (Clontech Laboratories ...
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bioRxiv - Molecular Biology 2019Quote: ... the cDNA was amplified by a polymerase chain reaction from a human brain cDNA library (Clontech, CA) using an appropriate primer pair ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plasmid GFP-GT198 contained full-length human GT198 in a pEGFP-C3 vector (Clontech, 6082-1). HeLa cells transfected with GFP-GT198 (green ...
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bioRxiv - Cancer Biology 2021Quote: shRNA directed against human MAGI1 and AMOTL2 were constructed and cloned in the pSIREN-RetroQ vector (TaKaRa) according to the manufacturer’s conditions between BamHI and EcoRI cloning sites as previously described for other genes 75 ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was generated from a human lung poly-A+ RNA (cat. 636105, Takara Bio, Kusatsu, Shiga, Japan) using a cDNA library construction kit (Takara Bio ...
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bioRxiv - Immunology 2020Quote: The pIRES-DsRed2 and pDsRed2-C1 vectors used to express various human aquaporin constructs were from Clontech. The human pCMV6-AC-AQP9-GFP expression vector was from Origene ...
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bioRxiv - Cell Biology 2021Quote: ... the coding sequences of human Pcnt B and Pcnt S were subcloned into peGFP-N1 (Takara Clontech) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... the coding sequences of human Pcnt B and Pcnt S were subcloned into peGFP-N1 (Takara Clontech) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Immunology 2022Quote: ... cloned into pTT3-based IgG expression vectors with human constant regions (84) using In-Fusion cloning (Clontech), expressed in 293 cells ...
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bioRxiv - Neuroscience 2024Quote: ... Human KCNQ2 cDNA (GenBank accession number NM_172108) in pIRES2-EGFP (BD Biosciences-Clontech, Mountain View, CA, USA) was used as template for in vitro mutagenesis ...
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bioRxiv - Cancer Biology 2021Quote: ... Supernatant was collected at 48- and 72-hours post transfection and virus was concentrated using Lenti-X™ concentrator (Takara) and resuspended in appropriate volumes of MEM ...
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bioRxiv - Developmental Biology 2021Quote: ... Lentiviral supernatant was harvested at 48 h and 54 h after transfection and concentrated using the lenti-X concentrator (Clontech) at 4°C for 12-36 h ...
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bioRxiv - Cell Biology 2020Quote: ... to a final concentration of 108-109 IFU/ml using the Lenti-x qRT-PCR Titration Kit (Takara, cat.631235). Lentivirus with MOI of 10 was used for transduction of MOVAS cells ...
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bioRxiv - Molecular Biology 2021Quote: The Tet-on vector was obtained from the Lenti-X Tet-One Inducible Expression System (Puro) (Clontech, Cat. No. 634847). We transfected 1 × 106 HDR-immortalized cells using the Neon nucleofection system and 10 μg of plasmid DNA (plasmid encoding Cre recombinase ...
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bioRxiv - Cancer Biology 2019Quote: ... NEK9 lentiviral particles for each construct were harvested from transfected 293T packaging cells using the Lenti-X(tm) HTX Packaging System (Clontech), transduced into U2OS parental cells and selected in fresh media supplemented with 2 mg/ml of G418 and 1 µg/ml puromycin over several days ...
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bioRxiv - Cancer Biology 2019Quote: ... Lentiviral particles were concentrated from supernatant by mixing 3 parts supernatant with 1 part Lenti-X concentrator solution (ClonTech 631231), incubating overnight at 4 °C ...
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Screening and identification of MicroRNAs expressed in perirenal adipose tissue during rabbit growthbioRxiv - Developmental Biology 2019Quote: ... Six DE miRNAs were reverse transcribed into cDNA using Mir-X™ miRNA First-Strand Synthesis Kits (Takara, Dalian, China) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... Viral titering was performed using 15 μL of unconcentrated virus or 1.5 μl of concentrated virus with the Lenti-X qRT-PCR Titration Kit (Clontech, 632165). Results were read on an ABI QuantStudio 6 RT-PCR machine.
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bioRxiv - Genomics 2020Quote: ... All lentiviral particles other than those used in the RAW cell transductions and Figure 2B (top panel) were further purified and concentrated with the Lenti-X Concentrator reagent (Takara) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... Lentivirus was concentrated from supernatant using ultracentrifugation and the genome copy number was calculated using the Clontech Lenti-X qRT-PCR Titration kit (Takara).
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bioRxiv - Neuroscience 2021Quote: ... Lentiviral collection and spindown was performed at 24 hours and 48 hours after initial change of medium with Lenti-X-concentrator (Clontech) according to manufacturer protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated from the media 1:10 in PBS using Lenti-X concentrator (Takara Bio, Cat. No. 631231), aliquoted and stored at −80°C for future use.
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bioRxiv - Cancer Biology 2020Quote: ... and VSV-G were co-transfected with each lentiviral vector (pCW-RELA-HA, pCW-C11orf95fus1-HA, or pCW-C11orf95-RELAfus1-HA) into Lenti-X 293T cells (Clontech), seeded the day before in 6-well plates at a concentration of 1.2×106 cells per well ...
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bioRxiv - Cancer Biology 2021Quote: ... The wild-type BTK construct was cloned in the base vector (Lenti-X Expression System Version EF1-a; Takara Bio). STable over-expression of BTK in ibrutinib-resistant DLBCL cells was performed by using lentiviruses expressing human wild-type BTK as described previously.[15]
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bioRxiv - Cell Biology 2021Quote: ... Lentiviral vectors expressing KDELR2 or a control empty vector were titered using the Lenti-X p24 rapid titer kit (Takara). 8 × 105 SH-SY5Y in 2 mL growth media were plated in a 6-well plate ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was isolated using the TRIzol method and the expression of miRNAs analyzed by qRT-PCR with the Mir-X miRNA qRT-PCR TB Green kit (Takara Bio Inc ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral supernatants were collected 48 h post-transfection and concentrated by adding one volume of Lenti-X Concentrator (Takara Bio) to three volumes of lentivirus-containing supernatant and incubating at 4°C overnight ...