Labshake search
Citations for Takara Bio :
1101 - 1150 of 2518 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and then gel purified using NucleoSpin® Gel & PCR Clean-up Midi (Takara # 740986.20) for deep sequencing using the CRISPR-seq platform117 ...
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bioRxiv - Zoology 2019Quote: ... High-fidelity Takara Ex Taq PCR reagents were employed (Takara Bio Inc., Otsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... PCR on the resulting cDNA was performed for 35 cycles using PrimeStar MAX (Takara). Then ...
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bioRxiv - Microbiology 2019Quote: ... PCR for SNVs at IDEXX BioAnalytics used LA Taq™ (TaKaRa Bio, Otsu, Japan) and 20 µM primer pairs ...
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bioRxiv - Microbiology 2019Quote: ... The purified PCR products were sequenced with a commercial sequencer (Takara Bio, Shiga, Japan) by fluorescent dye terminator sequencing ...
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bioRxiv - Molecular Biology 2019Quote: ... All constructions were obtained by assembling PCR fragments using InFusion® cloning kit (Takara). Using appropriate sets of oligonucleotides ...
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bioRxiv - Molecular Biology 2019Quote: ... all other PCR products were amplified with PrimeSTAR HS DNA polymerase (Clontech Laboratories, Inc.). DNA isolations and manipulations were carried out using standard protocols ...
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bioRxiv - Genetics 2020Quote: ... double strand DNA was synthesized by PCR using PrimeSTAR GXL DNA Polymerase (Takara Bio) using forward 5’-ATGTGGTCGCGCCTGGCCTTTTGTTG and reverse 5’-AAGCCCGGCTCTCCTTTTCCTGGAACAATCAT primers ...
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bioRxiv - Cell Biology 2020Quote: ... Relative quantification PCR were performed using TB Green Premix Ex Taq kit (Takara, RR820B) and the StepOnePlus Real-Time PCR System (ABI ...
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bioRxiv - Cell Biology 2021Quote: ... The PITCh-mNeonGreen-guideMYH9 was generated by PCR and In- Fusion cloning method (Takara) to insert mNeonGreen at the 3’ end of MYH9 coding sequence with 40-bp microhomologies in accordance with the previous reports (Sakuma et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Tagmented DNA was isolated by Nucleospin PCR clean-up (Takara Bio USA, Inc # 740609.250). Libraries were amplified for 8 cycles and purified using AMPure XP (Agencourt # A63880) ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNA was amplified using a fluorescent quantitative polymerase chain reaction (PCR) kit (TaKaRa) and an instrument (Applied Biosystems 7500 ...
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bioRxiv - Biochemistry 2021Quote: ... real-time polymerase chain reaction (PCR) amplification was performed with SYBR Premix ExTag (Takara) on a QuantStudio™ 3 System (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... PCR reactions for amplifying deletion cassettes were done using PrimeSTAR MAX DNA Polymerase (Takara). NAD+ and NADP+(Na)2 were purchased from Carl Roth GmbH ...
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bioRxiv - Microbiology 2020Quote: ... PCR amplification was achieved using TaKaRa Ex Taq DNA Polymerase (ClonTech, Mountain View, CA). This amplified PCR product was then cloned into the pGEM-T Easy Vector using T4 ligase (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... and Q263A mutants were constructed by inverse PCR using PrimeSTAR MAX (Takara, Tokyo, Japan). For crystallization ...
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bioRxiv - Immunology 2021Quote: ... Real-time PCR were performed with the SYBR Premix Ex Taq II (TAKARA#RR820A) in a LightCycler480 real-time PCR system ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time PCR (qPCR) was performed with TB Green Premix Ex Taq (Takara) using LightCycler 96 System (Roche Life Science ...
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bioRxiv - Immunology 2022Quote: ... by PCR and subcloned in pEGBacMam using In-Fusion seamless DNA cloning (Takara Bio). PCR was used to generate the pEGBacMam-Strep-FLAG-Strep-EROS construct ...
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bioRxiv - Genetics 2022Quote: ... Cas9 was amplified from the expression plasmid using CloneAmp HiFi PCR Premix (Takara Bio) for 35 cycles (98□ for 10 s ...
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bioRxiv - Bioengineering 2022Quote: ... 1 µl purified cDNA was amplified with an Advantage HF 2 PCR kit (Takara) in a 25 µl reaction containing 0.5 µl forward primer (10 µM ...
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bioRxiv - Microbiology 2022Quote: A DNA matrix (Supplementary Table 5) was prepared with CloneAmp HiFi PCR Premix (Takara) using primer pair 56/60 (Supplementary Table 4 ...
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bioRxiv - Genomics 2022Quote: ... PCR was performed to generate double-stranded cDNA using PrimeSTAR GXL DNA polymerase (Takara). Sequencing libraries of both species were then constructed using SMRTbell Express Template Prep Kit 2.0 and Sequel Binding Kit 3.0 (Pacific Biosciences ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR was performed with SYBR Premix Ex Taq™ II (Takara Bio Inc.) or THUNDERBIRD SYBR qPCR Mix (Toyobo ...
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bioRxiv - Microbiology 2022Quote: ... The amplified PCR products were cloned into the pMD18-T vector (TaKaRa, Dalian, China) and transformed into competent cells of Escherichia coli DH5alfa ...
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bioRxiv - Plant Biology 2022Quote: RT-PCR detection of SAUR30 and UBQ5 expression was performed using ExTaq polymerase (TaKaRa) and the gene-specific primers listed in Supplemental Table S1.
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bioRxiv - Plant Biology 2022Quote: ... Quantitative RT-PCR were carried out using TB Green Premix Ex Taq II (Takara). 1 μL of diluted cDNA was used for each reaction in a final volume of 15 μL ...
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bioRxiv - Neuroscience 2022Quote: ... The titer of AAV was determined with a realtime PCR thermal cycler (Dice, TAKARA). The resultant AAV1-Syn-mCherry (2.9 × 1012 GC/ml) ...
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bioRxiv - Molecular Biology 2023Quote: ... 14-3-3γ CDS was amplified by PCR and subcloned into pCMV-mCherry (Clontech). Deletion and point mutations of SMAUG1 were created using Q5 site-directed mutagenesis kit (NEB) ...
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bioRxiv - Neuroscience 2023Quote: ... All cloning reactions used PCR amplification and ligation-independent cloning (In-Fusion, Takara Inc.). Reactions involving non-AAV plasmids were transformed into Stellar competent cells (Takara Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... Synthesized cDNA was subjected to quantitative PCR using TB Green Fast qPCR Mix (Takara) on the LightCycler system (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... Real-time PCR was performed using TB Green Premix Ex Taq II (Takara, Japan) and ViiA7 Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... The edit locus was PCR amplified using Titanium® Taq DNA Polymerase (Takara, 639208) followed by Sanger Sequencing.
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bioRxiv - Physiology 2022Quote: ... PCR product were inserted into the vector backbone using In-fusion HD cloning (Takara) and transformed in Stbl3 competent cells and grown at 37°C overnight.
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bioRxiv - Molecular Biology 2022Quote: ... The sgRNA library was prepared using a one-step PCR with ExTaq polymerase (Takara) and a mixture of P5 forward primers with staggers from 1 to 8 bp and barcoded P7 reverse primers ...
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bioRxiv - Bioengineering 2024Quote: cDNA and the plasmid library were amplifed using CloneAmp PCR Master Mix (Takara 639298) and primers for amplicon sequencing (Supplemtary Note 23) ...
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bioRxiv - Cell Biology 2024Quote: ... qRT-PCR was performed with a SYBR Premix Ex Taq II Kit (Takara, Japan) by a Real-Time System (CFX96 ...
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bioRxiv - Cell Biology 2024Quote: ... qRT-PCR was performed with a SYBR Premix Ex Taq II Kit (Takara, Japan) by a Real-Time System (CFX96 ...
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bioRxiv - Immunology 2024Quote: ... and quantitative PCR (qPCR) was performed with SYBR Premix Ex Taq II (TaKaRa-Bio) using a LightCycler (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... qRT-PCR was carried out with TB Green Premix Ex Taq II (Takara: RR82LR) using gene-specific primers ...
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bioRxiv - Cell Biology 2024Quote: ... 100 ng of isolated gDNA was amplified using the PrimeSTAR MAX PCR mix (Takara) using the primers gRNA_screening_FW and gRNA_screening_RV (Table1) ...
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bioRxiv - Microbiology 2024Quote: ... Quantified PCR was performed using SYBR Premix Ex Taq Kit (Takara Bio Inc., Japan), and carried out on ABI Prism 7000 sequence detection system (Applied Biosystems USA) ...
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bioRxiv - Developmental Biology 2024Quote: ... The CDs of cdon was amplified using PCR (Prim STAR Max Premix Takara NO.R045A) and cloned into the vector PCS2+ to generate the expression constructs (5x In-Fusion HD Enzyme Premix ...
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bioRxiv - Cell Biology 2024Quote: All PCRs were performed in 50 µL reactions using ExTaq Polymerase (Takara Bio #RR001B) with the following program:
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bioRxiv - Microbiology 2024Quote: ... PCR amplification involved specific primers (Supplementary Table 1) and Ex Taq Polymerase (Takara, Japan) (Choi et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... The converted DNA was amplified by PCR using Ex Taq Hot Start Version (Takara). And the PCR products were subcloned into pEASY-T5 Zero Cloning Kit (TransGen ...
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bioRxiv - Cell Biology 2023Quote: ... using the One-Step TB Green PrimeScript RT-PCR Kit II (Takara, Kyoto, Japan). Preparation of PCR reactions was automated by the Echo 525 Acoustic Liquid Handler (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... mutagenesis was performed via overlapping PCR using PrimeSTAR GXL DNA polymerase (TaKaRa, Cat# R050A) with the primers listed in Supplementary Table 2 ...
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bioRxiv - Plant Biology 2023Quote: All qRT-PCR was performed using SYBR Green I (Takara SYBR Premix Ex Taq) on the Bio-Rad CFX96 Real-time PCR detection system.
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bioRxiv - Neuroscience 2023Quote: ... using the One-Step TB Green® PrimeScript™ RT-PCR Kit II (Takara) for RT-PCR ...