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Citations for Takara Bio :
1051 - 1100 of 2391 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: A DNA matrix (Supplementary Table 5) was prepared with CloneAmp HiFi PCR Premix (Takara) using primer pair 56/60 (Supplementary Table 4 ...
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bioRxiv - Genomics 2022Quote: ... PCR was performed to generate double-stranded cDNA using PrimeSTAR GXL DNA polymerase (Takara). Sequencing libraries of both species were then constructed using SMRTbell Express Template Prep Kit 2.0 and Sequel Binding Kit 3.0 (Pacific Biosciences ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR was performed with SYBR Premix Ex Taq™ II (Takara Bio Inc.) or THUNDERBIRD SYBR qPCR Mix (Toyobo ...
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bioRxiv - Microbiology 2022Quote: ... The amplified PCR products were cloned into the pMD18-T vector (TaKaRa, Dalian, China) and transformed into competent cells of Escherichia coli DH5alfa ...
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bioRxiv - Plant Biology 2022Quote: RT-PCR detection of SAUR30 and UBQ5 expression was performed using ExTaq polymerase (TaKaRa) and the gene-specific primers listed in Supplemental Table S1.
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bioRxiv - Plant Biology 2022Quote: ... Quantitative RT-PCR were carried out using TB Green Premix Ex Taq II (Takara). 1 μL of diluted cDNA was used for each reaction in a final volume of 15 μL ...
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bioRxiv - Neuroscience 2022Quote: ... The titer of AAV was determined with a realtime PCR thermal cycler (Dice, TAKARA). The resultant AAV1-Syn-mCherry (2.9 × 1012 GC/ml) ...
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bioRxiv - Molecular Biology 2023Quote: ... 14-3-3γ CDS was amplified by PCR and subcloned into pCMV-mCherry (Clontech). Deletion and point mutations of SMAUG1 were created using Q5 site-directed mutagenesis kit (NEB) ...
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bioRxiv - Neuroscience 2023Quote: ... All cloning reactions used PCR amplification and ligation-independent cloning (In-Fusion, Takara Inc.). Reactions involving non-AAV plasmids were transformed into Stellar competent cells (Takara Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... Synthesized cDNA was subjected to quantitative PCR using TB Green Fast qPCR Mix (Takara) on the LightCycler system (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... Real-time PCR was performed using TB Green Premix Ex Taq II (Takara, Japan) and ViiA7 Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... The edit locus was PCR amplified using Titanium® Taq DNA Polymerase (Takara, 639208) followed by Sanger Sequencing.
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bioRxiv - Physiology 2022Quote: ... PCR product were inserted into the vector backbone using In-fusion HD cloning (Takara) and transformed in Stbl3 competent cells and grown at 37°C overnight.
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bioRxiv - Molecular Biology 2022Quote: ... The sgRNA library was prepared using a one-step PCR with ExTaq polymerase (Takara) and a mixture of P5 forward primers with staggers from 1 to 8 bp and barcoded P7 reverse primers ...
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bioRxiv - Cell Biology 2023Quote: ... using the One-Step TB Green PrimeScript RT-PCR Kit II (Takara, Kyoto, Japan). Preparation of PCR reactions was automated by the Echo 525 Acoustic Liquid Handler (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... mutagenesis was performed via overlapping PCR using PrimeSTAR GXL DNA polymerase (TaKaRa, Cat# R050A) with the primers listed in Supplementary Table 2 ...
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bioRxiv - Plant Biology 2023Quote: All qRT-PCR was performed using SYBR Green I (Takara SYBR Premix Ex Taq) on the Bio-Rad CFX96 Real-time PCR detection system.
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bioRxiv - Neuroscience 2023Quote: ... using the One-Step TB Green® PrimeScript™ RT-PCR Kit II (Takara) for RT-PCR ...
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bioRxiv - Biochemistry 2023Quote: PCR reactions were performed with PrimeSTAR MAX DNA Polymerase (BD Clontech GmbH, Heidelberg, Germany) or Phusion High-Fidelity polymerase (Thermo Fisher Scientific GmbH ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... The target area was initially amplified by PCR using PrimeSTAR® GXL polymerase (Takara). Amplicons were amplified again by PCR with TruSeq DNA-RNA CD index-containing primers to label each fragment with adapter and index sequences in order to build NGS libraries ...
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bioRxiv - Microbiology 2023Quote: ... we performed mutagenesis by overlapping PCR using PrimeSTAR GXL DNA Polymerase (TaKaRa, Cat# R050A) together with the primers IFNAR-F (5′-CGTGGGCCCGCGGCCGCGCCACCATG-3’ ...
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bioRxiv - Physiology 2023Quote: ... DNA was amplified using Terra PCR Direct Red Dye Premix (Takara Cat. No. 639286) and ran on a 1% agarose gel to confirm DNA recombination ...
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bioRxiv - Plant Biology 2023Quote: ... Real-time PCR was performed on a thermal cycler Dice Real Time System (Takara) using KOD SYBR qPCR Kit (Toyobo ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: qRT-PCR was performed using SYBR® Premix Ex Taq™ II (Takara Biotechnology) and CFX96 Connect Real-Time system (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2023Quote: ... Real-time PCR was performed on a thermal cycler Dice Real Time System (Takara) using KAPA SYBR FAST qPCR Kit (KAPA Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... The qRT-PCR was performed using TB Green Premix Ex TaqTM (RR820A, Takara, Japan) with above cDNA as template ...
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bioRxiv - Immunology 2023Quote: ... PCR products were purified by gel electrophoresis and cloned into a T vector (Takara), and individual bacterial colonies were picked for sequencing ...
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bioRxiv - Developmental Biology 2023Quote: ... The PCR amplified cDNA fragments were cloned into PEGF-N1 vector (Clontech,6085-1,) with XhoI/BamhI enzyme sites ...
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bioRxiv - Plant Biology 2023Quote: The PCR amplified promoter sequence of AtPHT1;5 gene was cloned into pAbAi vector (Takara) between the SacI and KpnI RE sites following manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... and used as templates for real-time PCR analysis using SYBR PreMix ExTaqII (Takara). The primers used are listed in Supplementary Table S3 ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplifications were carried out with PrimeStar polymerase as recommended by the manufacturer (Takara). E ...
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bioRxiv - Cell Biology 2023Quote: ... Fragments of interest were amplified using the EmeraldAmp GT PCR Master Mix (Takara, #RR310B) and intron- or exon - specific forward primers combined with a common exon junction-specific reverse primer ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was prepared from total RNA using the PrimeScript RT-PCR kit (Takara, #RR037Q). Relative gene expression of the cDNA was assayed using a 7500 Fast real-time PCR instrument (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... and CEP44 mutants were amplified via PCR and cloned into pEGFP-N1 (Clontech Laboratories) and p3×FLAG-CMV-14 (Sigma-Aldrich) ...
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bioRxiv - Pathology 2023Quote: ... and reverse transcribed using PrimeScript RT Master Mix for RT-PCR (Takara Bio, Japan). Real-time PCR was performed using SYBR Select Master Mix (Applied Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... Purified PCR products were cloned into pYAO::hSpCas9 plasmid via In-Fusion (Takara, 639650).
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bioRxiv - Plant Biology 2023Quote: ... PCR was performed using primer GSP-HO1 and the Universal Primer Mix (UPM, Clontech) with KOD One PCR Master Mix (TOYOBO ...
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bioRxiv - Cancer Biology 2023Quote: ... and purified using the NucleoSpin® Gel and PCR Clean-Up kit (TaKaRa, 740609), according to the manufacturer’s protocols.
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bioRxiv - Plant Biology 2024Quote: ... The quantitative RT-PCR was performed on a Thermal Cycler Dice® TP800 (TaKaRa) with the SYBER® Premix Ex Taq TM II kit (TaKaRa ...
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bioRxiv - Plant Biology 2024Quote: The promoter sequences of STM-orthologs were isolated by TAIL-PCR (TAKARA, no.6108) from the DNA of the representative Brassicaceae species (Extended Data Table 1) ...
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bioRxiv - Cell Biology 2023Quote: ... PCR was performed removing the region of interest followed by In-Fusion (Takara 638909).
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bioRxiv - Developmental Biology 2023Quote: ... The converted DNA was amplified by PCR using Ex Taq Hot Start Version (Takara). And the PCR products were subcloned into pEASY-T5 Zero Cloning Kit (TransGen ...
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bioRxiv - Cell Biology 2024Quote: ... qRT-PCR was performed with a SYBR Premix Ex Taq II Kit (Takara, Japan) by a Real-Time System (CFX96 ...
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bioRxiv - Immunology 2024Quote: ... and quantitative PCR (qPCR) was performed with SYBR Premix Ex Taq II (TaKaRa-Bio) using a LightCycler (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... qRT-PCR was carried out with TB Green Premix Ex Taq II (Takara: RR82LR) using gene-specific primers ...
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bioRxiv - Bioengineering 2024Quote: cDNA and the plasmid library were amplifed using CloneAmp PCR Master Mix (Takara 639298) and primers for amplicon sequencing (Supplemtary Note 23) ...
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bioRxiv - Cell Biology 2024Quote: ... qRT-PCR was performed with a SYBR Premix Ex Taq II Kit (Takara, Japan) by a Real-Time System (CFX96 ...
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bioRxiv - Cell Biology 2024Quote: ... 100 ng of isolated gDNA was amplified using the PrimeSTAR MAX PCR mix (Takara) using the primers gRNA_screening_FW and gRNA_screening_RV (Table1) ...
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bioRxiv - Microbiology 2024Quote: ... Quantified PCR was performed using SYBR Premix Ex Taq Kit (Takara Bio Inc., Japan), and carried out on ABI Prism 7000 sequence detection system (Applied Biosystems USA) ...
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bioRxiv - Developmental Biology 2024Quote: ... The CDs of cdon was amplified using PCR (Prim STAR Max Premix Takara NO.R045A) and cloned into the vector PCS2+ to generate the expression constructs (5x In-Fusion HD Enzyme Premix ...