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Citations for Takara Bio :
8701 - 8750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and SPATA5 was amplified from cDNA using ExTaq (Takara) with primers designed in-house (Table S3 ...
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bioRxiv - Microbiology 2022Quote: ... the gene fragments of transcriptional regulators were cloned into the pColdII plasmid (Takara Bio, Kusatsu, Japan) and transformed into E ...
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bioRxiv - Microbiology 2022Quote: ... After degradation of DNA using recombinant DNase I (Takara Bio), an equal quantity of total RNA (500 ng ...
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bioRxiv - Microbiology 2022Quote: ... 1.5 U ExTaq polymerase (Takara, Madison, Wisc.), and made to a volume of 50 µl with sterile milliQ water ...
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bioRxiv - Microbiology 2022Quote: ... using specific primers flanked by 15bp overlapping regions from pL6-var2csa-promoter-deletion plasmid and pUF1_Cas9 (list of primers, see Supplementary Table S8) that allowed the cloning by infusion cloning (Clontech, Takara Bio USA ...
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bioRxiv - Immunology 2022Quote: ... TACI and BCMA-Emerald were generated using the In-Fusion HD Cloning Kit (Takara, #638933) and expressed in the pSFG retroviral backbone.
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bioRxiv - Immunology 2022Quote: ... Universal primer A mix (CLONTECH) and 5’-GGGRCCARKG GATAGACHGATGG-3’ (designed according to the C segment of mouse IgG antibody heavy chain sequence ...
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bioRxiv - Microbiology 2022Quote: ... US10 was cloned in frame with eGFP in the pCMS-eGFP vector (Clontech) using primers shown in Table 3 ...
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bioRxiv - Microbiology 2022Quote: ... using Ex Taq polymerase (Takara Bio, Tokyo, Japan) with the following cycling conditions ...
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bioRxiv - Microbiology 2022Quote: ... and DNA was eliminated by DNase I (Takara) treatment ...
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bioRxiv - Microbiology 2022Quote: ... and converted to cDNA with the PrimeScript RT Master Mix (Takara), following the instructions of the kit’s manufacturers for both procedures ...
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bioRxiv - Microbiology 2022Quote: ... The titer of the produced lentivirus was determined by using Lenti-X Gostix Plus Titer Kit (Takara, cat# 631281). To generate AMOT knockdown cell line ...
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bioRxiv - Microbiology 2022Quote: ... pEGFP-C1 (Clontech) vector ...
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bioRxiv - Microbiology 2022Quote: ... and purified with NucleoZOL (Takara Bio, Göteborg, Sweden). Ribosomal RNA depletion was performed with the NEBNext Bacteria kit (NEB ...
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bioRxiv - Microbiology 2022Quote: ... and Thermal Cycler Dice Real Time System II (Takara). Three biologically independent experiments were performed and used for the analysis.
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bioRxiv - Microbiology 2022Quote: ... using the PrimeScript RT reagent Kit with gDNA Eraser (Takara). Real-time qPCR experiments were performed as described above ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of DNA fragments of interest was performed by real-time qPCR using TB Green Fast qPCR Mix (Takara) and Thermal Cycler Dice Real Time System II (Takara) ...
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bioRxiv - Microbiology 2022Quote: ... The amplified PCR products were cloned into the pMD18-T vector (TaKaRa, Dalian, China) and transformed into competent cells of Escherichia coli DH5alfa ...
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bioRxiv - Microbiology 2022Quote: ... Viral load was measured by RT-qPCR using One-Step SYBR® Primescript(tm) RT-PCR kit II (Takara). CT values from serum samples were used to calculate serum viral load according to regression equation built by a set of standard viral RNA extracted from dilutions of known titre virus preparation ...
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bioRxiv - Immunology 2022Quote: ... B/B homodimerizer (Takara Bio, Cat#635058), b-estradiol (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... RetroNectin® Recombinant Human Fibronectin Fragment (Takara Bio, Cat#T100A), Recombinant Murine Flt3-Ligand (Peprotech ...
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bioRxiv - Immunology 2022Quote: ... SMARTseq v4 Ultra Low Input RNA kit for Sequencing (Cat. # 634890, Takara Bio, USA) was used to generate full-length cDNA prior to library preparation with the Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Immunology 2022Quote: ... The extracted RNAs were reversed transcribed into cDNA using a PrimeScrip RT reagent kit (Takara, Japan) and quantified using Coronavirus 2019-nCoV nucleic acid detection kit (fluorescent PCR method ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected and purified using a Talon NTA-cobalt affinity column (Takara Bio, Kusatsu, Shiga, Japan) with elution in 200 mM Imidazole ...
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bioRxiv - Microbiology 2022Quote: ... of the repair plasmids by In-Fusion (Clontech Laboratories, CA, USA) ligation independent cloning (LIC) ...
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bioRxiv - Microbiology 2022Quote: ... using the NucleoSpin® Microbial DNA Kit (TaKaRa Bio, Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The Apollo 324 NGS Library Prep System was used with the PrepX RNA-seq library preparation kit (Takara Bio, San Jose, CA). Sequencing was performed on Illumina NovaSeq 6000 platform (SP 100nt Lane ...
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bioRxiv - Microbiology 2022Quote: ... and RNA was converted to cDNA using Primescript RT reagent kit (Takara Bio) in 10 uL reactions following manufacturer protocols ...
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bioRxiv - Microbiology 2022Quote: ... sacB was amplified using pAJF067 as a template and primers listed in Supplementary Table 3 and were cloned into pDB60 digested with EcoR1 using recombination based cloning (In-Fusion, Takara). For dinB2 and dinB3 overexpression plasmids constructs ...
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bioRxiv - Microbiology 2022Quote: ... and a Thermal Cycler Dice Real Time System TP800 (TaKaRa, Japan). GAPDH was used as an internal control ...
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bioRxiv - Microbiology 2022Quote: ... qRT-PCR was performed using the One Step TB Green PrimeScript PLUS RT-PCR Kit (RR096A, TaKaRa, Japan) and a Thermal Cycler Dice Real Time System TP800 (TaKaRa ...
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bioRxiv - Microbiology 2022Quote: ... SMARTer® PCR cDNA synthesis kit (TaKaRa,cat 634926) and PrimeSTAR® GXL DNA polymerase (TaKaRa ...
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bioRxiv - Microbiology 2022Quote: ... then the His-tagged ORF8 protein produced in the culture supernatants was purified with a Talon resin (Clontech). The absence of endotoxin contamination was confirmed using the ToxinSensor chromogenic LAL Endotoxin Assay Kit (GeneScript).
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bioRxiv - Microbiology 2022Quote: ... the PCR fragments were mixed in equimolar amounts (0.1 pmoL each) and subjected to CPER reaction within 50 μL reaction volumes using PrimeSTAR GXL DNA polymerase (Takara Bio., Shiga, Japan) The cycling conditions were an initial 2 min of denaturation at 98°C ...
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bioRxiv - Microbiology 2022Quote: ... Complementary DNA (cDNA) was obtained by reverse transcription using a PrimeScript RT reagent kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and Takara (Takara, Dalian, China), respectively ...
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bioRxiv - Microbiology 2022Quote: ... and further treated with DNase I (TaKaRa) to eliminate residual DNA contained in the extracted samples ...
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bioRxiv - Microbiology 2022Quote: ... with the help of high fidelity PrimeSTAR GXL DNA Polymerase (Takara). The PCR amplicon was cloned in the pGEMT-easy plasmid (Stratagene ...
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bioRxiv - Microbiology 2022Quote: NK cell activity was quantified using the LDH Cytotoxicity Detection Kit (MK401, TaKaRa, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... cfrA and its flanking region were amplified by PCR with GXL polymerase (Takara, Tokyo, Japan) from C ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from HT-29 cells using the CellAmp Direct RNA Prep Kit for RT-PCR (3732; TaKaRa, Japan) after the introduction of genomic DNA or Poly (I ...
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bioRxiv - Microbiology 2022Quote: ... Lentiviral plasmids were packaged into pseudoparticles via triple-plasmid co-transfection into HEK293T cells (TaKaRa). pTsin or pWPI plasmids ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized by by SMARTer RACE reagent (CLONTECH). The primer used for reverse transcription was Oligo-dT ...
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bioRxiv - Immunology 2022Quote: ... Universal primer A mix (CLONTECH) and 5’-5’ s-CTTCAGAGGA AGGGTGGAAACAGG-3’ (designed according to the C segment of mouse IgG antibody light chain sequence ...
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bioRxiv - Immunology 2022Quote: ... were purchased as synthetic genes from Eurofins with appropriate restriction sites (HindIII and BamHI) and inserted into pEGFP-N1 (Clontech). Constructs to express peptides in the cytosol were created by inserting annealed oligonucleotides comprising a Kozak sequence ...
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bioRxiv - Microbiology 2022Quote: ... using specific primers flanked by 15bp overlapping regions from pL6-var2csa-promoter-deletion plasmid and pUF1_Cas9 (list of primers, see Supplementary Table S8) that allowed the cloning by infusion cloning (Clontech, Takara Bio USA, Mountain View, CA, USA). Plasmid integration was validated by PCR across the site of integration and whole genome sequencing.
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bioRxiv - Molecular Biology 2022Quote: ... The assembled plasmids were amplified by transformation of Stellar Competent Cells (Takara) and purified from overnight cultures in LB medium containing 100 μg/ml Ampicillin grown at 37°C by using the NucleoSpin Plasmid isolation kit (Macherey-Nagel) ...
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bioRxiv - Microbiology 2022Quote: ... The commercial expression vectors pDsRed2-Mito (Clontech Laboratories, Inc., CA, USA), pCMV6-RFP-MAP1LC3B (Origene ...
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bioRxiv - Microbiology 2022Quote: The threshold cycle (Ct) values were determined by the SmartChip Real-Time PCR software (Takara Bio). Standard curves were generated by plotting the Ct values vs ...
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bioRxiv - Microbiology 2022Quote: ... The barcoded HygR amplicon was then cloned using Infusion cloning kit (Takara Bio) as per the manufacturer’s instructions ...