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Citations for Takara Bio :
8951 - 9000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Real-time PCR was performed on an ABI Prism 7500 Fast Real-Time PCR System using the SYBR Premix Ex Taq kit (Takara Bio Inc., Shiga, Japan) according to the instructions ...
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bioRxiv - Plant Biology 2022Quote: ... All yeast media were purchased from Clontech.
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bioRxiv - Plant Biology 2022Quote: All yeast constructs were transformed into the Saccharomyces cerevisiae Y2H Gold strain (Clontech) using Yeastmaker™ Yeast Transformation System 2 (Clontech) ...
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bioRxiv - Plant Biology 2022Quote: ... using Yeastmaker™ Yeast Transformation System 2 (Clontech). Transformants were grown on minimal synthetic defined (SD ...
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bioRxiv - Plant Biology 2022Quote: ... For Y2H assays, cDNAs of mCherry and ZmSIPs (ZmSIP1, ZmSIP2, ZmSIP3) were cloned into the pGADT7 vector (Clontech) for expression via the GAL4 AD under constitutive ADH1 promoter ...
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bioRxiv - Plant Biology 2022Quote: The pGBKT7 and pGADT7 plasmids were co-transformed into yeast strain AH109 (Clontech) using the LiCl-PEG (polyethylene glycol ...
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bioRxiv - Plant Biology 2022Quote: ... which was then cloned into the Aor51HI site in pUGW2 35S 97 using the In-Fusion cloning kit (TaKaRa Bio). The 2.5-kb HindIII-SacI fragment in the resulting plasmid ...
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bioRxiv - Physiology 2022Quote: ... rRNA depleted RNA passing quality control was used in the SMARTer Stranded RNA-Seq Kit (Takara Bio USA). Following RNA incubation with stranded N6 primer ...
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bioRxiv - Neuroscience 2022Quote: ... mCherry (Clontech 632496; to amplify mCherry signal) and 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Plant Biology 2022Quote: ... Plasmid vectors were linearized to generate templates for viral RNA in vitro transcription (T7 RNA Polymerase System, Takara Bio, Shiga, Japan). RNA1 to RNA3 were equally mixed to infect 3-week-old N ...
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bioRxiv - Plant Biology 2022Quote: ... Target DNA was amplified by PCR using TaKaRa Ex Taq DNA Polymerase (TaKaRa Bio, Tokyo, Japan). DNA segments were purified using MonoFas DNA Purification Kit I (ANIMOS ...
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Plastid double-strand RNA transgenes trigger small RNA-based gene silencing of nuclear-encoded genesbioRxiv - Plant Biology 2022Quote: ... Reverse transcription (RT) was performed using Advantage RT-for-PCR kit (Takara, San Jose, CA) following the manufacturer’s instructions with 20 μM oligo (dT)18 ...
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bioRxiv - Plant Biology 2022Quote: ... (RT-qPCR) in the presence of SYBR Green (TaKaRa TB Green Primer Ex TaqII, #RR820Q) was carried out using a BioScript PCR kit (Bioline ...
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bioRxiv - Plant Biology 2022Quote: ... The anti-GFP antibody (TaKaRa, 632381), the anti-LhcB2 antibody (Agrisera ...
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bioRxiv - Plant Biology 2022Quote: ... using the In-Fusion cloning kit (Clontech).
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bioRxiv - Plant Biology 2022Quote: ... Proteins were purified by immobilized-metal affinity chromatography using TALON® metal affinity resin (Clontech), where filtered supernatant (0.45 μm filter ...
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bioRxiv - Plant Biology 2022Quote: Y2H was performed using the Matchmaker TM GAL4-based two-hybrid system (Clontech) as described in Schrumpfová et al ...
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bioRxiv - Plant Biology 2022Quote: ... the In-Fusion® Snap Assembly cloning kit (Takara Bio USA) was used ...
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bioRxiv - Microbiology 2022Quote: ... Then were reverse-transcribed into cDNA using PrimeScript RT Master Mix Kit (Takara). qPCR was performed by TB Green Premix Ex Taq (Takara ...
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bioRxiv - Microbiology 2022Quote: ... qPCR was performed by TB Green Premix Ex Taq (Takara, RR420Q,), with gene-specific primer pairs (Table S1) ...
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bioRxiv - Plant Biology 2022Quote: ... 2 μL of 5× PrimeSTAR GXL Buffer (Takara Bio, Kusatsu, Japan), 1.0 μL of PrimeSTAR GXL DNA Polymerase (1.25 U/μL) ...
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bioRxiv - Systems Biology 2022Quote: ... HEK293T Lenti-X cells (Takara, 632180), which were used to package lentivirus as described in the following section ...
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bioRxiv - Immunology 2022Quote: ... an amount of 1 ng of total RNA was used as input for the SMART-Seq® Stranded Kit (Cat. No. 634444; protocol version “022829”; low input option, Takara Bio USA, San Jose, USA). This kit can deal with degraded as well as high-integrity input RNA ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from LNs and used for RT-qPCR using a Prime Script™ II Strand cDNA Synthesis kit (Takara, Dalian, China), in accordance with the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... RNA quality was assessed using an Agilent Fragment Analyzer and ten nanograms of total RNA was used as input for cDNA synthesis using the Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Matched healthy donor RNA was used to generate targeted IgG and IgM AIRR-seq libraries using the SMARTer Human BCR IgG IgM H/K/L Profiling Kit (Takara Bio USA) according to the manufacturer’s instructions with no modifications ...
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bioRxiv - Genomics 2022Quote: ... coli cells (Takara Bio) and plasmid was prepared following standard protocols ...
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bioRxiv - Microbiology 2022Quote: ... using a Lenti-X Concentrator (Clontech Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... Ct values were obtained from PCR tests conducted as administrative tests at the Toyama Institute of Health using the SARS-CoV-2 direct detection RT-qPCR test kit from Takara Bio Inc ...
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bioRxiv - Biochemistry 2022Quote: ... in the bait vector pGBKT7 or were fused to the GAL4 activation domain (AD) in the prey vector pGADT7 (Clontech Laboratories). The yeast strain Y2H Gold (Clontech Laboratories ...
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bioRxiv - Immunology 2022Quote: ... and sequencing libraries were constructed using SMART-seq HT Plus kit (Takara Bio, Otsu, Japan). Sequencing was performed with 150 bp paired-end reads on the Illumina HiSeq X sequencer (Illumina ...
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bioRxiv - Immunology 2022Quote: Extracted RNA was thawed on ice and converted to first strand complementary DNA (cDNA) using the SMARTer RACE 5’/3’ Kit (Takara Bio USA), as described by the manufacturer and a custom oligonucleotide that contained the template switch oligo and a unique molecular identifier (5’ TSO-UMI ...
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bioRxiv - Immunology 2022Quote: ... with SYBR Premix Ex TaqTM II (Tli RNaseH Plus) (TaKaRa, Beijing, China) and Universal PCR Master Mix (Applied Biosystems™ ...
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bioRxiv - Microbiology 2022Quote: ... All cloning were performed using In-Fusion recombination-based cloning method (Takara) and plasmids were introduced into M ...
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bioRxiv - Genomics 2022Quote: ... Npm1/Flt3-ITD/Cas 9 DM murine AML cells were lentivirally-transduced using a Retronectin-transduction protocol (T100A; Takara Bio) with pHKO9 containing guide RNAs complementary to murine BAF complex members (Smarcb1 ...
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bioRxiv - Genomics 2022Quote: We performed PCR with CloneAmp HiFi PCR Mix (Takara Biosciences, Mountain View, CA) and added DMSO to a final concentration of 5-10% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... total RNA was cleaned by genomic removal and then reversely transcribed (Prime Script RT reagent Kit with gDNA Eraser, Takara, China). The rRNA removal ...
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bioRxiv - Microbiology 2022Quote: Vero cells were transduced with supernatants of Plat-GP cells cotransfected with pMDG (35) and pRetroX-Tet3G (TaKaRa), selected with 1 mg/mL G418 solution (Wako ...
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bioRxiv - Microbiology 2022Quote: ... the viral RNA was isolated and amplified by RT-PCR using the PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio) and specific primers ...
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bioRxiv - Genetics 2022Quote: RACE was conducted using SMARTer Kit (Takara, 634858) as the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... 1.5μL Titanium Taq DNA Polymerase (Clontech Takara, Cat# 639242), 8 μL dNTPs (Clontech Takara Cat# 4030) ...
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bioRxiv - Genetics 2022Quote: ... and Nucleospin Blood Midi kit (Takara Cat. #740954) (for CRISPR/Cas12a screening) ...
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bioRxiv - Genetics 2022Quote: ... 1.5μL Titanium Taq DNA Polymerase (Clontech Takara, Cat# 639242), 8 μL dNTPs (Clontech Takara Cat# 4030) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The ATP1A1 genes were inserted at the PPH promoter of vectors already containing the corresponding ATP1B1 genes using In-Fusion® HD Cloning Kit (Takara Bio; Cat#638910) and confirmed by sequencing ...
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bioRxiv - Genetics 2022Quote: ... gDNA was extracted using a Nucleospin Blood Maxi kit (Takara, Cat. #740950) (for CRISPR/Cas9 screening ...
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bioRxiv - Genomics 2022Quote: 0.25-0.5×106 DM Cas9 cells were lentivirally transduced using Retronectin-mediated (Takara Bio #T100A) transduction with pooled guide RNA libraries (Supplemental table 1) ...
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bioRxiv - Microbiology 2022Quote: ... and reverse transcription was performed with PrimeScript RT Reagent Kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... HeLa wild-type or HeLa-HSF1i cell monolayers were mock-transfected or co-transfected with HCoV genomic RNA (1 μg/ml) and pCMV-GFP vector (Clontech) using TransIT-mRNA Transfection Kit (Mirus Bio ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP8-11 (ref.65) (400 ng) using TransIT-LT1 (Takara, Cat# MIR2300). On day 3 (24 hours posttransfection) ...
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bioRxiv - Microbiology 2022Quote: ... the 3′ terminus of each strand of dsRNA was ligated with PC3-T7loop oligo (Table S1) using T4 RNA ligase (TaKaRa, China) at 16 °C for 16 h ...