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Citations for Takara Bio :
8901 - 8950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... The dimer was first digested by KpnI (TaKaRa, Kyoto, Japan) to release a monomer ...
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bioRxiv - Plant Biology 2022Quote: ... and subsequently with EcoRI and EcoRV (TaKaRa, Kyoto, Japan) to release a 0.3mer fragment ...
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bioRxiv - Neuroscience 2022Quote: Primary antibodies used in this study were rabbit anti-dsRed (Clontech, 632496, 1:200), mouse anti-GFAP (ZIRC ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were centrifuged at 16,000 RCF for 5 min at 4°C and the supernatant was reserved for DNA clean-up using NucleoSpin® Gel and PCR Clean-Up (740609, Takara) based on manufacture instructions or ethanol precipitation.
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bioRxiv - Neuroscience 2022Quote: ... or anti-DsRed (rabbit, ClonTech; 1:300) to stain against Tomato ...
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bioRxiv - Neuroscience 2022Quote: ... were amplified by PCR and inserted into a CAG-promoter plasmid backbone by In-Fusion HD (Takara). A plasmid encoding hWDR62-FLAG was generated by amplifying hWDR62-FLAG by PCR ...
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bioRxiv - Neuroscience 2022Quote: ... were amplified and purified by Nucleospin plasmid TF grade (Takara bio, Shiga, Japan). All plasmid sequences were verified using Sanger sequencing (3100 Genetic Analyzer ...
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bioRxiv - Neuroscience 2022Quote: ... DNA fragments of OR genes were amplified by PrimeStar MAX polymerase (Takara bio, Shiga, Japan). The fragments were mixed and amplified by PCR reaction to obtain full sequences ...
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bioRxiv - Neuroscience 2022Quote: The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara #634894) was used per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... anti-DsRed (1:500, Clontech), anti-Synapsin I (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... using Xfect™ Transfection Reagent (Takara Bio) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Samples were processed using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories, Inc.). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... libraries were prepared using the SMARTer® Stranded Total RNA-seq Kit v2 – Pico Input Mammalian (Takara, #634413). Samples were fragmented for 4 min @ 94°C and final amplification was performed using 16 cycles ...
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bioRxiv - Neuroscience 2022Quote: ... The SMARTer Ultralow RNA kit (Clontech, 634833) was used to reverse transcribe poly(A ...
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bioRxiv - Microbiology 2022Quote: ... culture supernatants were collected from each well to measure lactate dehydrogenase (LDH) release by using an LDH cytotoxicity assay (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... In-Fusion cloning (TaKaRa Bio) was performed using primers 1 and 2 with at least 15 bp complementary sequence for ligation into vector pGCP123 (50) ...
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bioRxiv - Immunology 2022Quote: ... Lentiviral transduction of murine alveolar macrophages was performed for 7 days in the presence of 5 μg/cm2 RetroNectin (Takara). Stable gene expression was confirmed by GFP signals using a BZ-X710 (KEYENCE ...
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bioRxiv - Immunology 2022Quote: ... The NTD domain was purified from clarified supernatants using 2 ml of cobalt resin (Takara Bio TALON), washing with 50 column volumes of 20 mM HEPES-HCl pH 8.0 and 150 mM NaCl and eluted with 600 mM imidazole ...
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bioRxiv - Immunology 2022Quote: ... Culture supernatants containing lentivirus were collected 24 and 48 h after transfection and concentrated by a Lenti-X concentrator (Takara) followed by passing through a 0.45-μm PES filter ...
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bioRxiv - Immunology 2022Quote: ... 2.1 (Takara, Shiga, Japan). In this experiment ...
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bioRxiv - Genomics 2022Quote: RNA was extracted using the RNAiso Plus (Takara, 9109) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... 56 plasmids in 293FT HEK cells using the CalPhos™ Mammalian Transfection Kit (Takara bio, 631312). Following 2 μM tamoxifen treatment ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 μL RNase inhibitor (TaKaRa Bio Inc., Japan) and 1 μL sample ...
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bioRxiv - Microbiology 2022Quote: ... All PCR reactions were performed on a Thermal Cycler Dice Real Time System III (Takara Bio, Inc.). Previously sequenced E ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-dsRed: (1:5000, Takara Bio, Clontech, Australia, Cat#: 632496), goat anti-mCherry (1:5000 ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-dsRed: (1:5000, Takara Bio, Clontech, Australia, Cat#: 632496), goat anti-mCherry (1:5000 ...
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bioRxiv - Neuroscience 2022Quote: ... Gαi2 and Trpc2 cDNAs were amplified by PCR from female rat VNOs (PrimeSTAR® GXL DNA Polymerase Takara, Japan). Vom1r68 was synthesized by BGI (China) ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-DsRed (1:500; TaKaRa #632496), rat anti-Cadherin DN (1:30 ...
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bioRxiv - Neuroscience 2022Quote: ... Pgcl2 and Pgcl3 cDNAs in male rat livers were amplified by PCR (PrimeSTAR DNA Polymerase Takara, Japan). The oligonucleotide primer sequences employed for this purpose are shown in Supplementary Table S1 ...
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bioRxiv - Neuroscience 2022Quote: ... incubated with Lenti-X concentrator (Clontech) for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Immunofluorescent staining was performed using primary antibodies (FOS, #2250, Cell Signaling Technology, 1:1000; GFP, GFP1020, Aves Laboratories, 1:1000; dsRed, 632496, Takara, 1:1000), antibodies were reacted with species-specific Alexa Fluor-488 ...
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bioRxiv - Physiology 2022Quote: ... samples were rRNA depleted utilizing the RiboGone Mammalian-Low Input Ribosomal RNA Removal Kit (Takara Bio USA Inc., Mountain View, CA, USA), and Agencourt AMPure XP SPRI beads (Beckman Coulter) ...
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bioRxiv - Neuroscience 2022Quote: ... and qRT–PCR was performed using SYBR Premix Ex Taq (TaKaRa) on an ABI system (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... SYBR Green Mix (TaKaRa) was used for quantitative PCR ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were used: DS Red (1:1000, Rabbit, Takara-Clontech), ChAT (1:300 ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were used: DS Red (1:1000, Rabbit, Takara-Clontech), ChAT (1:300 ...
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bioRxiv - Microbiology 2022Quote: ... monoclonal antibody (Clontech) and peroxidase-labeled anti-mouse IgG (H + L ...
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bioRxiv - Microbiology 2022Quote: ... The open reading frame encoding each protein was amplified from RIB40 genomic DNA with PrimeSTAR® HS DNA Polymerase (TaKaRa Bio, Otsu, Japan) for high-fidelity PCR ...
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bioRxiv - Microbiology 2022Quote: ... The amplified fragments were then fused with SmaI-linearized pUt-C-EGFP10 using the In-Fusion HD Cloning Kit (Clontech Laboratories, Mountain View, CA, USA). Gene sequences fused with the egfp gene were positioned in tandem between the linker sequences ...
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bioRxiv - Microbiology 2022Quote: ... single-cells were isolated using a BD FACS Aria III for sorting individual cells into 96-well plates prefilled with lysis buffer (0.26 μl of 10×Lysis buffer (Takara), 0.03 μl of RNase Inhibitor (100 U/μl ...
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bioRxiv - Microbiology 2022Quote: ... 0.03 μl of RNase Inhibitor (100 U/μl, Takara), 0.26 μl of DPBS (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... DNAse treated-RNA was reverse transcribed into cDNA using random hexamers and oligo (dT) primers with the Prime-Script RT Reagent Kit (Perfect Real Time from Takara Bio Inc., Otsu Shiga, Japan). Viral presence was assessed through RT-qPCR (StepOnePlus Real-Time ...
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bioRxiv - Neuroscience 2022Quote: ... and circularized with DNA Ligation Kit ‘Mighty Mix’ (Takara). The plasmid templates ...
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bioRxiv - Neuroscience 2022Quote: ... The full-length wild-type TRPV4 was subcloned into the PLVX-IRES-mCherry vector to generate TRPV4WT (Clontech, Catalog No. 631237). To generate the TRPV4FeRIC construct ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The pSLI-HA-FKBP-T2A-yDHOH-Ribozyme plasmid was generated in our laboratory from the pSLI plasmid32 by In-fusion® (Takara) insertion of five PCR fragments coding for ...
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bioRxiv - Plant Biology 2022Quote: ... using an In-Fusion HD cloning kit (Clontech Laboratories, USA). Targeting vectors were introduced into F1 sporelings of M ...
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bioRxiv - Plant Biology 2022Quote: ... using PrimeSTAR® Mutagenesis Basal Kit (TaKaRa, Japan) so as not to be targeted by CRISPR/Cas9 ...
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bioRxiv - Plant Biology 2022Quote: ... AscI and NotI (Takara Bio, Japan). After confirming their sequences ...
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bioRxiv - Plant Biology 2022Quote: Reverse transcription was conducted using PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Japan). Real-time PCR was carried out using a KAPA SYBR FAST qPCR Master Mix 2X (Kapa Biosystems ...
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bioRxiv - Plant Biology 2022Quote: ... 5µl of cDNA and 1x SYBR® Premix Ex Taq™ (Takara, Shiga, Japan) and followed amplification protocol consisting in a denaturing step at 95°C for 30 s ...