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Citations for Takara Bio :
8801 - 8850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... PCR was conducted with cDNA as the template using Tks Gflex DNA Polymerase (Takara Bio). Quantitative reverse transcription PCR (qRT-PCR ...
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bioRxiv - Plant Biology 2022Quote: ... the PCR products amplified by the primers spanning the introns were purified and cloned into pMD19-T vector (TaKaRa, D102A). About 10~20 clones of each PCR products were sequenced and aligned with the corresponding genes by SnapGene software.
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bioRxiv - Plant Biology 2022Quote: ... DNase I (Takara Bio, Dalian, China) was used to process the total RNA ...
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bioRxiv - Plant Biology 2022Quote: ... plasmids were transformed into yeast strain AH109 by the lithium chloride–polyethylene glycol method according to the manufacturer’s manual (Clontech). The transformants were selected on SD-Leu-Trp plates ...
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bioRxiv - Plant Biology 2022Quote: ... we used the pGADT7-SV40 T-Ag and pGBKT7-p53 supplied with the Matchmaker system (Clontech).
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of total RNA was used to synthesize first-strand cDNA with PrimeScript RT enzyme together with gDNA eraser (Takara, Japan). qRT□PCR was performed on a Roche LightCycler 480 (Roche Diagnostics ...
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bioRxiv - Systems Biology 2022Quote: ... The PCR products were subsequently cloned into pGEMHE Xenopus laevis oocyte expression vector using In-Fusion® HD cloning kit (TaKaRa Bio Inc, Kusatsu, JP) and the final sequence validated (Eurofin ...
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bioRxiv - Plant Biology 2022Quote: ... PrimeScript RT Master Mix (Takara Bio) was used for cDNA synthesis and quantitative RT-PCR was performed with PowerUp SYBR Green Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesized by reverse transcription using PrimeScript RT Master Mix (Takara Bio Group ...
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bioRxiv - Plant Biology 2022Quote: ... ligated into the BamHI and KpnI restriction sites of the pOPINF vector91 using the In-Fusion kit (Clontech Takara) and transformed into chemically competent E ...
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bioRxiv - Plant Biology 2022Quote: ... ligated into the BamHI and KpnI restriction sites of the pOPINF vector91 using the In-Fusion kit (Clontech Takara) and transformed into chemically competent E ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were then treated with 200nM rapalog (A/C heterodimerizer, previously AP21967) (Takara Bio., 635055) and immediately subjected to live cell microscopy with images being taken every 30 minutes for 12 hours ...
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bioRxiv - Plant Biology 2022Quote: ... and forty five metabolic genes were cloned into pTRV2 vector (Burch-Smith et al., 2006) using In-Fusion cloning (Takara Korea). Both pTRV1 (contaning a gene encoding viral RNA-depenent RNA polymerase ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 ul recombinant RNase inhibitor (Takara, 2313B). Isolated nuclei were sorted on a MA900 Multi-Application Cell Sorter (Sony Biotechnology) ...
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bioRxiv - Neuroscience 2022Quote: ... and RNase inhibitor (Takara, 2313B). One male and one female sample from the same time point and region were pooled at this stage by FACS collecting into the same sample tube (thus yielding 50,000 nuclei per tube) ...
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bioRxiv - Plant Biology 2022Quote: Y2H assays were performed according to the manufacturer’s protocol (Clontech). For Y2H screening ...
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bioRxiv - Plant Biology 2022Quote: ... and then treated with RNase-free DNase I (Takara 2270B) to remove the potential DNA contamination ...
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bioRxiv - Plant Biology 2022Quote: Y2H protein interaction assays were performed according to the manufacturer’s instructions as described in the Yeast Protocols Handbook (Clontech; TaKaRa Bio USA ...
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bioRxiv - Plant Biology 2022Quote: ... bHLH39-1 and bHLH39-2 fused to the BD were co-transformed into yeast Y2HGold (Clontech). Transformants were grown on SD-Leu-Trp plates ...
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bioRxiv - Plant Biology 2022Quote: We use the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio USA) to detect protein–protein interactions between Pik-HMAs and AvrPik effectors ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA encoding the Piks-HMAs in pGBKT7 was co-transformed with either the individual AvrPik variants in pGADT7 into chemically competent Saccharomyces cerevisiae Y2HGold cells (Takara Bio USA). The single colonies grown on selection plates were inoculated in SD-Leu-Trp plate and grown at 30 °C for overnight ...
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bioRxiv - Plant Biology 2022Quote: ... cDNAs were synthesized using 3 µg each of the total RNA and PrimeScript 1st strand cDNA synthesis kit (Takara, Kusatsu, Shiga, Japan). Exon spanning primes were made ...
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bioRxiv - Plant Biology 2022Quote: ... then RNA was reverse-transcribed into cDNA using PrimeScript RT reagent with gDNA Eraser (Takara, Kusatsu, Japan). TB Green (Takara ...
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bioRxiv - Systems Biology 2022Quote: ... with 1 μg of total RNA as input for the SMARTer Apollo NGS library prep system (Takara, Mountain View, CA). The library for RNA-seq was prepared by using NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs ...
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bioRxiv - Systems Biology 2022Quote: ... media supplemented with 10% FBS (Takara, 632180), and 1% Penicillin Streptomycin (Gibco ...
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bioRxiv - Systems Biology 2022Quote: ... HEK293T-LentiX (Takara Bio, 632180, female) cells ...
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bioRxiv - Plant Biology 2022Quote: ... for MBP fusion using infusion cloning (Takara Korea). The constructrs were transfromed into E.coli BL21 (DE3 ...
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bioRxiv - Plant Biology 2022Quote: ... TB Green (Takara, Kusatsu, Japan) was then used in Step One Real-Time PCR System (Applied biosystem ...
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bioRxiv - Plant Biology 2022Quote: ... expression vector (Clontech) by the gene-specific primers (Supplemental Table S8 ...
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bioRxiv - Plant Biology 2022Quote: The coding sequence of ZmEREB57 was obtained and digested by EcoRI and BamHI and inserted into the pGADT7-AD vector (Clontech) containing the GAL4 active domain ...
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bioRxiv - Plant Biology 2022Quote: ... Reverse transcription was carried out with PrimeScript RT Master Mix (Takara). All steps were done as indicated in the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2022Quote: ... The location of T-DNA in mt2 was confirmed by using the GenomeWalker kit (Clontech Laboratories, Mountain View, CA) following the manufacturer’s recommended protocols ...
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bioRxiv - Plant Biology 2022Quote: ... Each of the up-regulated genes (MpGSTF7, MpGSTF10, MpGSTF15, MpCYP813A5, MpCYP822A1) was PCR amplified from genomic Tak-2 DNA using CloneAmp HiFi Premix (Takara Bio). The primers used to amplify gene sequences are listed below ...
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bioRxiv - Plant Biology 2022Quote: ... eGFP was immuno detected with anti-GFP antibody (1:4000 dilution) (JL-8, #632380, Takara) and anti- mouse-HRP (1:15000 dilution ...
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNA removal and first-strand cDNA synthesis were carried out using a TAKARA first-strand cDNA synthesis kit (TaKaRa, Japan). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Systems Biology 2022Quote: ... cDNA was synthesized from 1 μg of total RNA using the PrimeScript RT reagent kit (Perfect Real Time) (Cat No RR037A, TaKaRa) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... media supplemented with 10% FBS (Takara, 632180) and 1% Penicillin Streptomycin Glutamine (Gibco ...
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bioRxiv - Plant Biology 2022Quote: ... nls-edCitrineT9 was amplified from nlsABACUS1-2μ plasmid 14 and introduced into the pENTR221-f1-ABA-CUS2-truncation vectors using In-Fusion cloning (Takara) to generate pENTR-nlsABACUS2-100n and pENTR-nlsABACUS2-400n ...
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bioRxiv - Plant Biology 2022Quote: ... Bound proteins were detected using Hyper HRP Substrate (Takara). PtIns(3,5)P2 Grip protein (P-3516-3-EC ...
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bioRxiv - Plant Biology 2022Quote: ... using SYBR Premix Ex Taq™ (TaKaRa, Kusatsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: Yeast-two-hybrid library screening was performed according to the Matchmaker Gold Yeast Two-Hybrid System User Manual (Clontech).
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bioRxiv - Plant Biology 2022Quote: ... were chemically synthesised and cloned by PCR into the pJET1.2 based gRNA vectors using Clontech CloneAmp HiFi PCR Premix (Takara, Japan). The transformation vector pICU2:Cas9-dsRED containing the Cas9 gene from Streptococcus pyogenes expressed under the promoter of the Arabidopsis Incurvata 2 gene (ICU2 ...
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNA removal and reverse transcription were performed with PrimeScript TM RT regent kit (Takara). qPCR reaction was performed using iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... Detection of EGT2 toxicity on yeast growth was performed according to Matchmaker® Gold Yeast Two-Hybrid System User Manual (Takara). Yeast cells expressing pGBKT7-EGT2 displayed a similar growth rate to yeast cells transformed with an empty pGBKT7 vector ...
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bioRxiv - Plant Biology 2022Quote: ... which was conducted with the “Make Your Own “Mate & Plate™” Library System” according to the manufacturer’s instructions with minor modifications (Clontech). Oligo-dT primers provided in the kit were used for first-strand cDNA synthesis with 2 µg total RNA as input ...
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bioRxiv - Plant Biology 2022Quote: ... prey plasmids from library were rescued from yeast clones with “Easy Yeast Plasmid Isolation Kit” (Clontech) and sequenced ...
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bioRxiv - Plant Biology 2022Quote: ... His (SD/-Trp/-Leu/-Ade/-His) and with sprayed 100 µl of 4 mg/ml X-α-Gal (Takara Bio, CA, USA) in dimethylformamide on plates (SD/-Trp/-Leu/-His/X-α-Gal) ...
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bioRxiv - Neuroscience 2022Quote: ... and were routinely tested for mycoplasma contamination (assayed with PCR Mycoplasma Detection Set, TaKaRa, Cat. no. 6601) and pluripotency status with antibodies against OCT4 (rabbit ...
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bioRxiv - Plant Biology 2022Quote: ... using the In-Fusion Snap Assembly Kit (Takara Bio). The bait and different combinations of prey vectors were cotransformed into yeast strain AH109 using a modified yeast transformation protocol (James et al. ...
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bioRxiv - Plant Biology 2022Quote: ... The genomic fragments were assembled and subcloned into a modified version of pBluescript II KS (StrataGen, pBlueMLAPUCAP) using the InFusion® HD Cloning Plus kit (Clontech), yielding the construct pBS-TPS02r and pBS-TPS02g ...