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Citations for Takara Bio :
8851 - 8900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... and introduced into the yeast strain AH109 (Clontech), which was then transformed with a pACT2 carrying cDNA expression library prepared from dark-grown Arabidopsis cell suspension (89) ...
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bioRxiv - Plant Biology 2022Quote: ... -3636 to +21 bp relative to the inferred initiation codon) and CYP735A4 promoter (proCYP735A4; -3623 to +21 bp) were amplified with PrimeSTAR GXL DNA polymerase (Takara) and the specific primer sets proCYP735A3-F/R and proCYP735A4-F/R ...
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bioRxiv - Plant Biology 2022Quote: Nuclear two-hybrid interaction assays were performed using the MATCHMAKER GAL4 two-hybrid system (Clontech). The CWLP coding region without signal peptide (SP ...
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesized with the PrimeScript RT Reagent Kit (TaKaRa, Shiga, Japan), and qRT-PCR was performed on the Thermal Cycler Dice® (TaKaRa ...
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bioRxiv - Plant Biology 2022Quote: ... 3XHA or 3XFLAG fragments were inserted into the vector using HindIII sites and the In-Fusion kit (Clontech) to generate basic and control effector vectors p35S::HA or p35S::FLAG ...
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bioRxiv - Plant Biology 2022Quote: ... first-strand cDNA was synthesized with the PrimeScript RT Reagent Kit (TaKaRa, Shiga, Japan), and genomic DNA isolated from the WT ...
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bioRxiv - Neuroscience 2022Quote: ... prepared freshly with 940 nL of Lysis Buffer 1 (1 μL of 10X reaction buffer is diluted in 2.75 μL of water) (SMART-Seq v4 Ultra Low input RNA kit; Takara Bio Inc.) per well ...
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bioRxiv - Neuroscience 2022Quote: ... with RNAse-free water with 0.4 U/μL Recombinant RNase Inhibitor (Takara Clontech, Ref. 2313A) and a fresh dilution of 1 in 300,000 was prepared before the first strand synthesis.
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bioRxiv - Plant Biology 2022Quote: ... backbone with the FLAG and SV40 NLS (for version1) or without the FLAG and SV40NLS (for version 2) were amplified and assembled by TAKARA in-fusion HD cloning kit (cat639650 ...
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bioRxiv - Plant Biology 2022Quote: ... aril and kernel tissues were pooled equally and cDNA was synthesized using the SMARTer PCR cDNA Synthesis Kit (Clontech). Size fractionation and selection (1-2 ...
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT Master Mix Kit (Takara). SYBR Premix Ex Taq™ kit (Takara ...
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bioRxiv - Plant Biology 2022Quote: ... various guide RNA cassettes were cloned into the SpeI site of the obtained vectors by TAKARA in-fusion reaction or by restriction digestion and ligation of PCR amplified or IDT synthesized DNA fragments ...
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bioRxiv - Plant Biology 2022Quote: ... SYBR Premix Ex Taq™ kit (Takara) was used to perform quantitative real-time PCR ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were amplified and synthesized into cDNA according to the manufacturer’s protocol using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio Inc., Shiga, Japan) and amplification yield was checked using a Bioanalyzer (Agilent High Sensitivity DNA Kit ...
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bioRxiv - Neuroscience 2022Quote: ... with RNAse-free water with 0.4 U/μL Recombinant RNase Inhibitor (Takara Clontech, Ref. 2313A) and a fresh dilution of 1 in 300,000 was prepared before the first strand synthesis.
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bioRxiv - Neuroscience 2022Quote: ... qPCR expression analysis was conducted on cDNA derived from immunoprecipitated RNA of OT:RiboTag mice following reverse (SMARTer® PCR cDNA synthesis kit; Takara Bio Inc., Shiga, Japan).
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bioRxiv - Neuroscience 2022Quote: ... Single-indexed libraries were generated using ThruPLEX® DNA-seq Kit (Takara Bio Inc., Shiga, Japan), pooled and checked again using a Bioanalyzer (Agilent High Sensitivity DNA Kit ...
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bioRxiv - Plant Biology 2022Quote: ... NLS and FLAG tag coding sequence were amplified from HBT_pcoCASphi_version1 and version2 plasmids and cloned into the digested vector together with PCR amplified pUB10 and Rbcs E9 terminator by TAKARA in-fusion HD cloning kit (cat639650) ...
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bioRxiv - Plant Biology 2022Quote: ... 500 ng of RNA was treated with DNaseI (Takara) and then used for RT reactions using the PrimeScript RT Reagent Kit (Takara) ...
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bioRxiv - Plant Biology 2022Quote: ... and then used for RT reactions using the PrimeScript RT Reagent Kit (Takara), with an equimolar mix of random hexamers and polyA primers ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative RT-PCR were carried out using TB Green Premix Ex Taq II (Takara). 1 μL of diluted cDNA was used for each reaction in a final volume of 15 μL ...
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bioRxiv - Plant Biology 2022Quote: ... the full-length cDNA for PME17 was amplified by PCR using TakaRa Ex Taq DNA polymerase (TAKARA, Kyoto), and specific primers flanked by Gateway adapters ...
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bioRxiv - Plant Biology 2022Quote: ... Transformants were selected on Synthetic Defined (SD) media lacking Leucine and Tryptophane (Clontech). Three individual clones were grown ON in liquid cultures (lacking Leucine and Tryptophane ...
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bioRxiv - Plant Biology 2022Quote: ... The amplified fragments were cloned into the Xba I/Sac I site of pBI121 (Clontech) located downstream of the cauliflower mosaic virus (CaMV ...
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bioRxiv - Plant Biology 2022Quote: ... The CWLP coding region without signal peptide (SP) was cloned in frame downstream of the GAL4 DNA-binding domain in the bait vector pGBKT7 (Clontech) and introduced into the yeast strain AH109 (Clontech) ...
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bioRxiv - Plant Biology 2022Quote: ... without DNA contamination upon DNase I treatment (Takara). After quality control (OD26/280=1.8-2.2 ...
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bioRxiv - Plant Biology 2022Quote: ... 10 µl of RNA extraction mixture was then added to 1 µl of 10 mM dNTPs (Takara Catalog # 4030) and 100 mM of oligo-dT(18 ...
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bioRxiv - Plant Biology 2022Quote: ... 10 μl SYBR Premix Ex Taq II (Takara, Japan), and nuclease-free water ...
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bioRxiv - Plant Biology 2022Quote: ... The expression analysis of each target gene was performed quantitatively by real-time RT-PCR method after cDNA synthesis using PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa) and TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Plant Biology 2022Quote: ... and TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) (TaKaRa) with Thermal cycler (CFX96 ...
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bioRxiv - Plant Biology 2022Quote: ... dNTP (Takara Bio), and the primers designed for the above-mentioned FWA regions (see Supplementary Table 5 ...
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bioRxiv - Plant Biology 2022Quote: ... using the In-Fusion HD Cloning Kit (Takara Bio) after HindIII/BamHI double digestion of the vector ...
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bioRxiv - Plant Biology 2022Quote: ... and RabA4B were sequentially cloned into pCAMBIA1300 vector using In-Fusion cloning technology (Takara Bio) to construct UBQ10pro:mCherry:RabA4B:HSPter cassette ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR reaction was performed with a Biorad CFX384TM Real Time System PCR machine and forward and reverse gene-specific primers (0.5 μM, Table S2) using the SYBR® Premix Ex Taq™ (Tli RNaseH Plus) from Takara Bio ...
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bioRxiv - Plant Biology 2022Quote: ... Vector pCSpe was digested with HindIII (TAKARA) and PmacI (TAKARA ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was prepared by the phenol extraction procedure (Hanzawa et al., 1997) and reverse transcribed using PrimeScript reverse transcriptase (Takara, Kyoto, Japan). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Plant Biology 2022Quote: ... and a Gateway® cassette amplified from vector pGWB14 (kindly provided by Pinghua Li, Shandong Agricultural University, Shandong, China) was ligated using the In-Fusion kit (Clontech, USA) for generation the binary destination vector pCSpeGH (Sequence deposited in NCBI Gene Bank with the accession number MW186668) ...
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bioRxiv - Plant Biology 2022Quote: ... pOsSHR2::OsSHR2 or pOsPIN5c::OsPIN5c and inserted into the KpnI digested vector pCam23A (Sun et al., 2016) backbone by using the In-Fusion kit (Clontech), with primers are listed in Supplementary Table 4.
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bioRxiv - Plant Biology 2022Quote: ... pCambia1391Z was digested with SacII (TAKARA, Japan), then ligated with a SpeI DNA fragment amplified from vector pCHF3 (kindly provided by Zhihong Lang ...
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bioRxiv - Plant Biology 2022Quote: ... and PmacI (TAKARA) and a Gateway® cassette amplified from vector pGWB14 (kindly provided by Pinghua Li ...
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The Arabidopsis MCTP member QUIRKY regulates the formation of the STRUBBELIG receptor kinase complexbioRxiv - Plant Biology 2022Quote: The Matchmaker yeast two-hybrid system (Takara Bio Europe ...
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bioRxiv - Neuroscience 2022Quote: ... These plasmids were obtained by cloning cDNA extracted from mouse cerebellum in pAAV-MCS Expression Vector with In-Fusion Cloning system (Takara). 96h after transfection ...
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bioRxiv - Neuroscience 2022Quote: The jGCaMP7f fragment was generated using PCR with PrimeSTAR Max DNA Polymerase (TaKaRa, Shiga, Japan) on a PCR thermal cycler (TP350 ...
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bioRxiv - Neuroscience 2022Quote: ... FOXG1 (Takara; ABM227), and Alexa 488- ...
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bioRxiv - Neuroscience 2022Quote: ... on a PCR thermal cycler (TP350, TaKaRa)57,58 ...
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bioRxiv - Neuroscience 2022Quote: ... The titer of AAV was determined with a realtime PCR thermal cycler (Dice, TAKARA). The resultant AAV1-Syn-mCherry (2.9 × 1012 GC/ml) ...
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bioRxiv - Neuroscience 2022Quote: ... mCherry of pmCherry-C1 plasmid (Clontech) was subcloned into pAAV-hSyn-EGFP (Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... and In-Fusion cloning (In-Fusion Snap Assembly Master Mix, Takara Bio USA) were used to generate all plasmids and intermediates ...
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bioRxiv - Neuroscience 2022Quote: ... VTA slices were incubated with rabbit anti-dsRed polyclonal antibody (632496, Takara) as the primary antibody (1:1000 dilution ...
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bioRxiv - Neuroscience 2022Quote: The dn-VAMP2/3 constructs were assembled by InFusion cloning (Takara Bio) to anneal three DNA fragments in a pAAV vector backbone ...